FastQCFastQC Report
Sat 14 Jan 2017
SRR2933047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595592
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145352.4404290185227473No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC105701.774704831495386No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG33380.5604507783852033No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT15760.2646106730782146No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG12840.21558382248250482No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCGTCGCATTCG10490.1761272817633548No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA9150.15362865854477561No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG9010.1512780561189539No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT7680.12894733307364772No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCGTCGCATT7650.1284436325538288No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.10829561176107134No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG6320.10611290950852262No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC6290.10560920898870368No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC6040.10141170465687921No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG206.308992E-446.0000041
ACCGGTA206.308992E-446.00000441
CGAAATG206.308992E-446.00000446
ACGGGCA405.6043064E-946.0000045
TATAGCG351.0184522E-746.0000041
TATCGCG206.308992E-446.0000041
TAAGACG351.0184522E-746.0000041
CAATAAG206.308992E-446.0000041
TAAACGG253.41516E-546.02
ACGGGTC301.8598821E-646.05
ACAGGCG501.6370905E-1146.033
ACGTCCC253.41516E-546.043
CGTAAGG501.6370905E-1146.02
TCGAACC253.41516E-546.014
TACGTAG253.41516E-546.01
CGTTTTT43700.044.052631
TACGGGA950.043.578954
TAGGGAC1550.043.032265
TAGCGAG750.042.9333341
GGCACTA601.8189894E-1242.1666688
Produced by FastQC (version 0.11.5)