##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933047.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595592
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.494773267606014	33.0	31.0	34.0	31.0	34.0
2	32.88004540020685	34.0	31.0	34.0	31.0	34.0
3	32.866913927655176	34.0	31.0	34.0	31.0	34.0
4	36.33691352469476	37.0	37.0	37.0	35.0	37.0
5	36.21079363053903	37.0	37.0	37.0	35.0	37.0
6	36.298783395344465	37.0	37.0	37.0	35.0	37.0
7	36.52763301051726	37.0	37.0	37.0	35.0	37.0
8	36.49094514365539	37.0	37.0	37.0	35.0	37.0
9	38.30907231796263	39.0	39.0	39.0	37.0	39.0
10	37.99826559121009	39.0	39.0	39.0	35.0	39.0
11	37.66428192453895	39.0	37.0	39.0	35.0	39.0
12	37.26877123937192	39.0	35.0	39.0	35.0	39.0
13	37.16594581525608	39.0	35.0	39.0	34.0	39.0
14	37.84499120203092	40.0	37.0	41.0	32.0	41.0
15	38.18358540746014	40.0	37.0	41.0	33.0	41.0
16	38.45451584306035	41.0	37.0	41.0	34.0	41.0
17	38.50870898198767	41.0	36.0	41.0	35.0	41.0
18	38.382768741017344	40.0	36.0	41.0	35.0	41.0
19	38.26648779701541	40.0	36.0	41.0	35.0	41.0
20	38.030114575078244	40.0	35.0	41.0	34.0	41.0
21	37.99654461443404	40.0	35.0	41.0	34.0	41.0
22	37.96358413141883	40.0	35.0	41.0	34.0	41.0
23	37.9391596932128	40.0	35.0	41.0	34.0	41.0
24	37.88200479522895	40.0	35.0	41.0	34.0	41.0
25	37.72339789654663	40.0	35.0	41.0	34.0	41.0
26	37.630267028435576	40.0	35.0	41.0	34.0	41.0
27	37.51798210855753	39.0	35.0	41.0	34.0	41.0
28	37.46911140512297	39.0	35.0	41.0	34.0	41.0
29	37.39552411718089	39.0	35.0	41.0	34.0	41.0
30	37.31017038509584	39.0	35.0	41.0	33.0	41.0
31	37.11069154723368	39.0	35.0	41.0	33.0	41.0
32	36.9121814933713	39.0	35.0	41.0	33.0	41.0
33	36.72514405834867	39.0	35.0	41.0	32.0	41.0
34	36.45234993082513	39.0	35.0	41.0	31.0	41.0
35	36.32289218122473	39.0	35.0	41.0	31.0	41.0
36	36.230385901758254	39.0	35.0	41.0	31.0	41.0
37	36.07885767438112	39.0	35.0	41.0	30.0	41.0
38	36.029051766981425	39.0	35.0	41.0	30.0	41.0
39	35.99518966003573	39.0	35.0	41.0	30.0	41.0
40	35.90334826525541	39.0	35.0	41.0	30.0	41.0
41	35.73157967199022	39.0	35.0	41.0	28.0	41.0
42	35.78045541242999	39.0	35.0	41.0	29.0	41.0
43	35.74191728565864	38.0	35.0	41.0	29.0	41.0
44	35.69943854182058	38.0	35.0	41.0	29.0	41.0
45	35.64435553197491	38.0	35.0	41.0	29.0	41.0
46	35.60875062123064	38.0	35.0	41.0	29.0	41.0
47	35.51317176859327	38.0	35.0	41.0	29.0	41.0
48	35.3772213192924	37.0	35.0	41.0	28.0	41.0
49	35.348559080712974	37.0	35.0	41.0	28.0	41.0
50	35.24679478569222	37.0	35.0	40.0	27.0	41.0
51	35.16531618960631	37.0	35.0	40.0	27.0	41.0
52	34.90454539349085	36.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	0.0
12	1.0
13	3.0
14	9.0
15	10.0
16	49.0
17	76.0
18	179.0
19	301.0
20	554.0
21	880.0
22	1415.0
23	2267.0
24	3783.0
25	6258.0
26	8312.0
27	8810.0
28	8795.0
29	8694.0
30	9030.0
31	10587.0
32	12754.0
33	17642.0
34	47473.0
35	62534.0
36	38572.0
37	41478.0
38	66308.0
39	237502.0
40	1314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.18732958132413	21.898044298781716	26.426815672473776	17.487810447420383
2	40.64712084782872	22.67961960536743	23.87775524184341	12.795504304960442
3	31.63004204220339	22.27145428414082	32.55466829641768	13.543835377238109
4	28.677349595024783	25.227840535131435	31.818929737135488	14.275880132708297
5	24.795665489126783	29.69012344020739	31.414122419374337	14.100088651291486
6	27.27941275235396	35.370857902725355	28.727551746833402	8.622177598087282
7	83.76724334779514	1.7374309930287848	12.716423323348868	1.7789023358272107
8	82.55416459589786	1.9832368467004258	12.922436835954814	2.5401617214468963
9	77.18270225254872	3.913920939166409	14.682366452202178	4.2210103560826875
10	47.085252991981086	22.02682373168209	19.71970745073809	11.168215825598732
11	30.72254160566294	23.48033553170627	31.307002108826175	14.490120753804616
12	30.067730929898318	18.375666563687894	34.34935324853255	17.207249257881234
13	29.098611129766688	18.945351851603114	37.405136402100766	14.550900616529436
14	18.26619565071391	20.55299601069188	42.86038093191312	18.320427406681084
15	14.907520584561244	26.987602251205523	41.21563083453102	16.88924632970221
16	28.672480489999863	24.348043627181024	32.39449153111526	14.58498435170385
17	26.802744160431974	22.4915714113017	30.089054251903992	20.616630176362342
18	22.871361603245173	23.07720721567785	36.36818493196685	17.68324624911013
19	22.26742467998227	24.625246813254712	31.85519617456245	21.252132332200567
20	17.18743703743502	35.49728673319991	30.47807895337748	16.837197275987588
21	22.81192494190654	24.976493975741786	37.64221144676221	14.569369635589464
22	19.043069752447987	21.65526064822899	41.207403726040646	18.09426587328238
23	19.96232320111754	22.754838882993727	39.01143735980335	18.271400556085375
24	20.88711735550511	27.76229365068705	33.00867036494782	18.341918628860025
25	23.49393544574138	25.155139760104234	33.4816451530578	17.86927964109659
26	17.74016440784967	28.17280957433948	29.777767330655884	24.309258687154966
27	21.305356687128103	24.445761528025898	35.13059275477172	19.11828903007428
28	16.443471369662454	27.896781689478704	31.055487649263252	24.60425929159559
29	20.329688780238822	28.31082351676987	30.244529812354763	21.114957890636543
30	25.190902497011376	23.250983895015377	30.397654770379724	21.16045883759352
31	21.971248774328735	24.37709035715725	34.07181426211232	19.5798466064017
32	24.95768915633521	23.644206100820696	28.857338580773416	22.540766162070682
33	26.041820575158837	23.427111176778734	30.624487904471522	19.906580343590914
34	19.0168773254174	23.484533036038094	36.2224475815659	21.276142056978603
35	20.133077677336164	29.055124984888987	28.976715603970504	21.835081733804348
36	25.20584561243267	25.746652070544933	27.912564305766363	21.134938011256025
37	19.469871992907898	29.443813886015928	29.525245470053324	21.561068651022847
38	22.856754288170425	25.941584171714865	30.412429985627742	20.789231554486964
39	20.64181520235329	27.009597173904282	28.20219210466225	24.146395519080173
40	25.299030208599177	25.203830810353395	29.61305726067509	19.884081720372336
41	19.348480167631532	25.91186584104555	27.95823315289661	26.781420838426307
42	18.07327835162326	27.20671197732676	33.95730634394015	20.762703327109833
43	19.072955983290573	26.421107066582493	32.94151029563863	21.564426654488308
44	23.73184999126919	25.188551894585554	29.076280406721377	22.003317707423875
45	19.429408051149107	24.39119397171218	28.388897097341804	27.79050087979691
46	22.03135703636046	24.076884847345163	29.671822321320633	24.21993579497374
47	18.13153971174898	25.024513425297854	33.48668215825599	23.357264704697176
48	18.378688766806807	30.3518180230762	29.945499603755593	21.3239936063614
49	23.17106341253744	25.65380327472498	30.403363376271002	20.771769936466576
50	20.300977850609144	25.596549315638896	29.930052787814475	24.172420045937486
51	19.947212185522975	26.485244932772773	28.77758599846875	24.789956883235504
52	19.820447554701875	27.345061720103697	29.432732474579915	23.401758250614517
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	559.0
1	1711.5
2	2864.0
3	10545.5
4	18227.0
5	10802.0
6	3377.0
7	3506.0
8	3635.0
9	4280.0
10	4925.0
11	5611.5
12	6298.0
13	6384.0
14	6189.5
15	5909.0
16	5436.5
17	4964.0
18	4859.5
19	4755.0
20	4483.5
21	4212.0
22	4174.5
23	4137.0
24	4293.5
25	4450.0
26	5079.0
27	5708.0
28	6652.5
29	7597.0
30	8289.0
31	8981.0
32	9913.0
33	10845.0
34	11857.0
35	12869.0
36	13434.0
37	13999.0
38	15604.0
39	17379.5
40	17550.0
41	19277.5
42	21005.0
43	23136.0
44	25267.0
45	27569.5
46	29872.0
47	33091.5
48	36311.0
49	42388.0
50	48465.0
51	49499.5
52	50534.0
53	50406.0
54	50278.0
55	45359.5
56	40441.0
57	37055.0
58	33669.0
59	34390.5
60	35112.0
61	26455.0
62	17798.0
63	14921.0
64	10475.0
65	8906.0
66	7327.5
67	5749.0
68	4763.0
69	3777.0
70	3306.5
71	2836.0
72	2247.5
73	1659.0
74	1293.0
75	927.0
76	799.0
77	671.0
78	482.5
79	294.0
80	277.5
81	261.0
82	174.5
83	88.0
84	49.5
85	11.0
86	27.0
87	43.0
88	29.0
89	10.0
90	5.0
91	7.5
92	10.0
93	6.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	595592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.93181487405774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87878834642477	24.000307660700972
2	9.469464511440567	6.236933044944407
3	3.725607881578711	3.680730871484416
4	1.9831017621622766	2.6122856043178313
5	1.3347151900431258	2.197729677404651
6	1.0838429341233589	2.1415748915463606
7	0.8735242555156166	2.0136717349447575
8	0.7865545482293729	2.0722135016510266
9	0.6955994113161166	2.0616615935969307
>10	7.0838787654626865	43.1085318981578
>50	0.046042261646453114	0.9816910295059815
>100	0.02967167972768145	2.087378042829628
>500	0.0061389682195203	1.3548582905920796
>1k	0.0020463227398401	1.2209224113306143
>5k	0.0	0.0
>10k+	0.00102316136992005	4.229509746992559
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14535	2.4404290185227473	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	10570	1.774704831495386	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3338	0.5604507783852033	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	1576	0.2646106730782146	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	1284	0.21558382248250482	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCGTCGCATTCG	1049	0.1761272817633548	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	915	0.15362865854477561	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	901	0.1512780561189539	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	768	0.12894733307364772	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCGTCGCATT	765	0.1284436325538288	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	645	0.10829561176107134	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	632	0.10611290950852262	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	629	0.10560920898870368	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	604	0.10141170465687921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09620679928541687	0.0
2	0.0	0.0	0.0	0.3707235825867372	0.0
3	0.0	0.0	0.0	0.6665636878937259	0.0
4	0.0	0.0	0.0	1.023183655925533	0.0
5	0.0	0.0	0.0	2.174139343711803	0.0
6	0.0	0.0	0.0	3.043190640572741	0.0
7	0.0	0.0	0.0	3.62933014546871	0.0
8	0.0	0.0	0.0	4.697343147658128	0.0
9	0.0	0.0	0.0	5.326968797431799	0.0
10	0.0	0.0	0.0	6.151526548375398	0.0
11	0.0	0.0	0.0	7.498421738371234	0.0
12	0.0	0.0	0.0	8.327176993646658	0.0
13	0.0	0.0	0.0	8.715362194253785	0.0
14	0.0	0.0	0.0	9.132929925183683	0.0
15	0.0	0.0	0.0	9.44337734556542	0.0
16	0.0	0.0	0.0	9.84465875968784	0.0
17	0.0	0.0	0.0	10.32267055299601	0.0
18	0.0	0.0	0.0	10.929461779204557	0.0
19	0.0	0.0	0.0	11.276847237706349	0.0
20	0.0	0.0	0.0	11.783905760990745	0.0
21	0.0	0.0	0.0	12.206846297465379	0.0
22	1.6790017327297883E-4	0.0	0.0	12.671761877258257	0.0
23	1.6790017327297883E-4	0.0	0.0	13.094198713213071	0.0
24	1.6790017327297883E-4	0.0	0.0	13.43906566911577	0.0
25	1.6790017327297883E-4	0.0	0.0	13.805927547717229	0.0
26	1.6790017327297883E-4	0.0	0.0	14.13971309218391	0.0
27	1.6790017327297883E-4	0.0	0.0	14.681191150989267	0.0
28	1.6790017327297883E-4	0.0	0.0	15.060813442759473	0.0
29	1.6790017327297883E-4	0.0	0.0	15.553096750795847	0.0
30	3.3580034654595765E-4	0.0	0.0	16.487461215059973	0.0
31	3.3580034654595765E-4	0.0	0.0	16.950026192427032	0.0
32	3.3580034654595765E-4	0.0	0.0	17.300265953874465	0.0
33	3.3580034654595765E-4	0.0	0.0	17.720016387056912	0.0
34	3.3580034654595765E-4	0.0	0.0	18.16411234536394	0.0
35	3.3580034654595765E-4	0.0	0.0	18.593433088422948	0.0
36	3.3580034654595765E-4	0.0	0.0	18.918991524399253	0.0
37	3.3580034654595765E-4	0.0	0.0	19.334208652903328	0.0
38	3.3580034654595765E-4	0.0	0.0	19.796605730097113	0.0
39	3.3580034654595765E-4	0.0	0.0	21.263213743636584	0.0
40	5.037005198189364E-4	0.0	0.0	21.6856505795914	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	6.308992E-4	46.000004	1
ACCGGTA	20	6.308992E-4	46.000004	41
CGAAATG	20	6.308992E-4	46.000004	46
ACGGGCA	40	5.6043064E-9	46.000004	5
TATAGCG	35	1.0184522E-7	46.000004	1
TATCGCG	20	6.308992E-4	46.000004	1
TAAGACG	35	1.0184522E-7	46.000004	1
CAATAAG	20	6.308992E-4	46.000004	1
TAAACGG	25	3.41516E-5	46.0	2
ACGGGTC	30	1.8598821E-6	46.0	5
ACAGGCG	50	1.6370905E-11	46.0	33
ACGTCCC	25	3.41516E-5	46.0	43
CGTAAGG	50	1.6370905E-11	46.0	2
TCGAACC	25	3.41516E-5	46.0	14
TACGTAG	25	3.41516E-5	46.0	1
CGTTTTT	4370	0.0	44.05263	1
TACGGGA	95	0.0	43.57895	4
TAGGGAC	155	0.0	43.03226	5
TAGCGAG	75	0.0	42.933334	1
GGCACTA	60	1.8189894E-12	42.166668	8
>>END_MODULE