##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1489446
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.504168664053616	33.0	31.0	34.0	31.0	34.0
2	32.89640980606212	34.0	31.0	34.0	31.0	34.0
3	32.92829548704686	34.0	31.0	34.0	31.0	34.0
4	36.379623699012924	37.0	37.0	37.0	35.0	37.0
5	36.29239059354955	37.0	37.0	37.0	35.0	37.0
6	36.37994395231516	37.0	37.0	37.0	35.0	37.0
7	36.555519971855304	37.0	37.0	37.0	35.0	37.0
8	36.51567092731123	37.0	37.0	37.0	35.0	37.0
9	38.34333101032196	39.0	39.0	39.0	37.0	39.0
10	38.03506538672768	39.0	39.0	39.0	35.0	39.0
11	37.727261679846066	39.0	38.0	39.0	35.0	39.0
12	37.442621619044935	39.0	37.0	39.0	35.0	39.0
13	37.38462354459309	39.0	37.0	39.0	35.0	39.0
14	38.343373979318486	41.0	38.0	41.0	33.0	41.0
15	38.56575733527768	41.0	38.0	41.0	33.0	41.0
16	38.76900270301844	41.0	38.0	41.0	35.0	41.0
17	38.797285030810116	41.0	38.0	41.0	35.0	41.0
18	38.69234198487223	40.0	38.0	41.0	35.0	41.0
19	38.618336616433226	40.0	37.0	41.0	35.0	41.0
20	38.45409836946086	40.0	36.0	41.0	35.0	41.0
21	38.41527453831827	40.0	35.0	41.0	35.0	41.0
22	38.37113396524614	40.0	35.0	41.0	35.0	41.0
23	38.33484731907031	40.0	35.0	41.0	35.0	41.0
24	38.29222543146915	40.0	35.0	41.0	35.0	41.0
25	38.182827709094525	40.0	35.0	41.0	34.0	41.0
26	38.10118258735127	40.0	35.0	41.0	34.0	41.0
27	38.008121140343455	40.0	35.0	41.0	34.0	41.0
28	37.97160286442073	40.0	35.0	41.0	34.0	41.0
29	37.9140975906478	40.0	35.0	41.0	34.0	41.0
30	37.86426295414537	40.0	35.0	41.0	34.0	41.0
31	37.737374836012854	40.0	35.0	41.0	34.0	41.0
32	37.60631738243615	40.0	35.0	41.0	34.0	41.0
33	37.4641094742609	40.0	35.0	41.0	33.0	41.0
34	37.2564094300834	40.0	35.0	41.0	33.0	41.0
35	37.14047437772165	40.0	35.0	41.0	33.0	41.0
36	37.058689606739684	40.0	35.0	41.0	33.0	41.0
37	36.94216507345684	40.0	35.0	41.0	32.0	41.0
38	36.91988296319571	40.0	35.0	41.0	33.0	41.0
39	36.878516575961804	40.0	35.0	41.0	32.0	41.0
40	36.826552959959606	40.0	35.0	41.0	32.0	41.0
41	36.71428772845743	40.0	35.0	41.0	32.0	41.0
42	36.725260935945315	40.0	35.0	41.0	32.0	41.0
43	36.68182062323844	40.0	35.0	41.0	32.0	41.0
44	36.64478067684226	39.0	35.0	41.0	32.0	41.0
45	36.59102713357853	39.0	35.0	41.0	32.0	41.0
46	36.538225622144076	39.0	35.0	41.0	32.0	41.0
47	36.45930030360282	39.0	35.0	41.0	31.0	41.0
48	36.35033495675573	39.0	35.0	41.0	31.0	41.0
49	36.30265212703247	39.0	35.0	41.0	31.0	41.0
50	36.19936338746084	39.0	35.0	41.0	31.0	41.0
51	36.12149886602133	38.0	35.0	41.0	31.0	41.0
52	35.888513581559856	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	5.0
14	10.0
15	21.0
16	69.0
17	177.0
18	316.0
19	588.0
20	1032.0
21	1592.0
22	2483.0
23	4254.0
24	6697.0
25	10892.0
26	14637.0
27	15819.0
28	15969.0
29	16575.0
30	18256.0
31	21618.0
32	27233.0
33	37809.0
34	96917.0
35	141544.0
36	93422.0
37	102214.0
38	168659.0
39	686689.0
40	3944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.23803749850615	22.840975772199865	25.885127758911704	15.035858970382277
2	36.88364667131269	23.540161912550037	26.42868556496845	13.147505851168823
3	29.741326640912124	23.74473461944911	32.778966139087956	13.73497260055081
4	26.763239486359357	27.573876461449427	31.549045752581833	14.113838299609386
5	24.808552978758545	32.02506166722392	28.6844907435382	14.481894610479332
6	26.764112294101295	39.59781019251453	25.001443489727055	8.636634023657118
7	87.60948701732053	1.4918298481448808	9.06874099497397	1.8299421395606152
8	86.41985006505774	1.84968102233985	9.250352144354345	2.48011676824806
9	81.26263053511171	4.003971946616393	11.251028906049632	3.482368612222263
10	43.91183030469047	27.71668123584205	16.3856897128194	11.985798746648083
11	27.148147700554436	24.191075070865274	33.43739887179529	15.223378356785005
12	28.57478552428218	19.87074388732455	33.355086387824734	18.199384200568534
13	27.909571746810556	20.960142227378505	34.716196491849985	16.414089533960947
14	18.153192529302842	23.206010825501565	37.56571235210944	21.075084293086157
15	14.778380686510287	29.726018935899656	38.06059434178883	17.43500603580123
16	26.44761877906282	27.72030674492395	31.041138785830434	14.790935690182794
17	24.89395385935442	26.521337463728123	29.874530530143424	18.710178146774034
18	20.775174125144517	25.779316604965874	34.719083471304096	18.726425798585513
19	20.76476757129832	28.0641929952479	30.559483190394282	20.6115562430595
20	17.599228169399897	35.820096868231545	29.649816106122678	16.930858856245877
21	22.12084224604316	28.133681919317656	34.573458856514435	15.172016978124752
22	20.01811411759809	24.655073094291435	36.11074184629721	19.216070941813264
23	19.06097971997642	25.6685371607967	36.25119675369231	19.01928636553457
24	19.662612810400645	28.678246811230483	32.79017836161901	18.86896201674985
25	21.81086121954069	27.975770857083777	31.661637951291954	18.55172997208358
26	18.475862837591965	30.67496236855851	28.644341587409013	22.204833206440515
27	19.926335026580354	27.92944490770394	32.879943280924586	19.26427678479112
28	17.09239542756166	30.392239799227365	29.5864368362465	22.928927936964485
29	19.580636021715456	29.145534648453182	29.058253874259286	22.215575455572072
30	23.0507181864935	27.23569703097662	28.7669375056229	20.946647276906987
31	21.080925391051437	27.685931547702967	30.979236575209846	20.253906486035746
32	23.717677579449003	27.17822599812279	27.820679635246933	21.283416787181274
33	23.74896438004466	27.21797231990955	28.939552021355592	20.093511278690197
34	19.945469657845937	26.828700067004778	32.37499043268437	20.850839842464914
35	20.186498872735232	30.49402260974886	28.62903388239654	20.690444635119366
36	23.504309656073467	29.49882036676724	26.733899718418797	20.2629702587405
37	20.80176119174512	30.471329608458447	28.838306323290674	19.88860287650576
38	22.34548953100683	28.39894833381002	30.14489951297328	19.11066262220987
39	20.444715686235014	29.670226379472638	28.29971680745727	21.58534112683508
40	24.04498048267611	27.242813771026274	30.23238170433839	18.479824041959226
41	20.244507018045635	27.533995861548522	28.57022006840127	23.65127705200457
42	19.394056582111737	28.774054245672552	31.059534887468228	20.772354284747482
43	19.635488631343467	28.722558588898156	30.642399925878482	20.999552853879898
44	22.02409486480208	27.995845435148368	28.416337349591725	21.56372235045782
45	19.364985370399467	27.89251842631421	27.58300737321125	25.159488830075077
46	21.205871176262853	27.891041367058623	28.49999261470372	22.403094841974802
47	18.429738305383346	27.282291536584747	31.58261528111795	22.705354876913965
48	19.40553736087109	29.802154626619558	29.44363206185387	21.34867595065548
49	21.979380252791977	27.872779543534982	29.689495288852363	20.458344914820678
50	19.960441667573043	27.98470035167438	29.61268820756174	22.442169773190837
51	19.451527614965563	28.259567651328076	28.793457433166424	23.495447300539933
52	19.581844524742756	29.31962622344147	29.12069319733646	21.977836054479315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1064.0
1	2294.0
2	3524.0
3	20332.0
4	37140.0
5	21384.0
6	5628.0
7	6026.5
8	6425.0
9	8287.0
10	10149.0
11	12370.0
12	14591.0
13	15375.0
14	15357.0
15	14555.0
16	13266.0
17	11977.0
18	11097.5
19	10218.0
20	9940.5
21	9663.0
22	10379.0
23	11095.0
24	11819.5
25	12544.0
26	14342.0
27	16140.0
28	18591.0
29	21042.0
30	23524.0
31	26006.0
32	29213.5
33	32421.0
34	37489.5
35	42558.0
36	45882.5
37	49207.0
38	52041.0
39	58051.5
40	61228.0
41	65381.5
42	69535.0
43	73538.5
44	77542.0
45	81486.5
46	85431.0
47	95605.5
48	105780.0
49	116995.5
50	128211.0
51	124415.0
52	120619.0
53	115561.0
54	110503.0
55	97382.0
56	84261.0
57	75888.0
58	67515.0
59	67457.0
60	67399.0
61	52494.0
62	37589.0
63	30799.0
64	19997.0
65	15985.0
66	13523.0
67	11061.0
68	8632.0
69	6203.0
70	4951.5
71	3700.0
72	3279.0
73	2858.0
74	2115.5
75	1373.0
76	1166.5
77	960.0
78	627.0
79	294.0
80	257.5
81	221.0
82	170.0
83	119.0
84	70.5
85	22.0
86	29.5
87	37.0
88	19.5
89	2.5
90	3.0
91	3.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1489446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.38050362138812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63095880297325	16.031369328934463
2	10.487716848503839	4.694407698160668
3	4.339298073483091	2.913470287436124
4	2.308939000896657	2.067008706845276
5	1.4321993127067647	1.6026670952291662
6	0.961945582084168	1.2917295950047818
7	0.7351148322840089	1.151656811624787
8	0.5751357010331755	1.0297461311810063
9	0.46190376656823706	0.9303875028371917
>10	5.120540549076889	26.851940159081796
>50	1.5140844222156695	23.945770725102243
>100	0.4231220389667909	12.02872487344812
>500	0.004821903577291605	0.7882635377153032
>1k	0.0033150587093879785	1.1913996004280925
>5k	3.013689735807253E-4	0.4568920342674308
>10k+	6.027379471614506E-4	3.0245659127036317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31033	2.0835263581224157	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	13810	0.9271903781674529	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	6774	0.4547999726072647	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2209	0.14831017707254912	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	2014	0.1352180609434649	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	1903	0.1277656256084477	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	1797	0.12064888555879166	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	1635	0.10977235831309091	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1525	0.10238706203514596	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09661310312693444	0.0
2	0.0	0.0	0.0	0.27835853062145255	0.0
3	0.0	0.0	0.0	0.4878995277438726	0.0
4	0.0	0.0	0.0	0.7331585032287172	0.0
5	0.0	0.0	0.0	1.51412001509286	0.0
6	0.0	0.0	0.0	2.4163346640294443	0.0
7	0.0	0.0	0.0	3.1536557887966397	0.0
8	0.0	0.0	0.0	4.359137558528473	0.0
9	0.0	0.0	0.0	5.095787292724946	0.0
10	6.713905707222685E-5	0.0	0.0	5.903805844589196	0.0
11	6.713905707222685E-5	0.0	0.0	6.910690283501382	0.0
12	6.713905707222685E-5	0.0	0.0	7.491577405290289	0.0
13	6.713905707222685E-5	0.0	0.0	7.769264545341019	0.0
14	6.713905707222685E-5	0.0	0.0	8.094217581570597	0.0
15	6.713905707222685E-5	0.0	0.0	8.293486302960966	0.0
16	6.713905707222685E-5	0.0	0.0	8.569360688470747	0.0
17	6.713905707222685E-5	0.0	0.0	8.905660225345532	0.0
18	6.713905707222685E-5	0.0	0.0	9.264585624453655	0.0
19	6.713905707222685E-5	0.0	0.0	9.518438399243745	0.0
20	6.713905707222685E-5	0.0	0.0	9.832044934828117	0.0
21	6.713905707222685E-5	0.0	0.0	10.141623126988156	0.0
22	6.713905707222685E-5	0.0	0.0	10.492626117361757	0.0
23	6.713905707222685E-5	0.0	0.0	10.82469589364099	0.0
24	6.713905707222685E-5	0.0	0.0	11.071431928381426	0.0
25	6.713905707222685E-5	0.0	0.0	11.331125801136798	0.0
26	6.713905707222685E-5	0.0	0.0	11.559331456125298	0.0
27	6.713905707222685E-5	0.0	0.0	11.863404245605413	0.0
28	6.713905707222685E-5	0.0	0.0	12.112288730172159	0.0
29	1.342781141444537E-4	0.0	0.0	12.429453635781357	0.0
30	1.342781141444537E-4	0.0	0.0	13.178658373650338	0.0
31	1.342781141444537E-4	0.0	0.0	13.537785189929679	0.0
32	1.342781141444537E-4	0.0	0.0	13.885095532164309	0.0
33	2.0141717121668058E-4	0.0	0.0	14.195949366408719	0.0
34	2.0141717121668058E-4	0.0	0.0	14.51499416561594	0.0
35	2.0141717121668058E-4	0.0	0.0	14.83638883182069	0.0
36	2.0141717121668058E-4	0.0	0.0	15.106354980308115	0.0
37	2.0141717121668058E-4	0.0	0.0	15.41163627281553	0.0
38	2.0141717121668058E-4	0.0	0.0	15.775999935546505	0.0
39	2.0141717121668058E-4	0.0	0.0	16.97550632919891	0.0
40	2.0141717121668058E-4	0.0	0.0	17.367061310044136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	6.3120597E-4	46.0	36
GCGAACA	20	6.3120597E-4	46.0	9
ATTACGC	25	3.4176468E-5	46.0	33
TCGATCA	20	6.3120597E-4	46.0	41
TATTGCG	40	5.6134013E-9	46.0	1
AACGGTT	30	1.8617775E-6	46.0	41
CCGTACG	25	3.4176468E-5	46.0	1
CTACGTC	25	3.4176468E-5	46.0	13
CCGCGTG	20	6.3120597E-4	46.0	35
CGATCCG	20	6.3120597E-4	46.0	40
CCACGCG	20	6.3120597E-4	46.0	1
TATCGGC	20	6.3120597E-4	46.0	35
ATCGCTC	25	3.4176468E-5	46.0	31
TACCGTA	20	6.3120597E-4	46.0	43
TAGTACG	40	5.6134013E-9	46.0	1
ACGGTTC	20	6.3120597E-4	46.0	11
ACGGTAC	25	3.4176468E-5	46.0	32
GAATCGC	35	1.0198346E-7	45.999996	18
CGTTTTT	7810	0.0	44.704224	1
TTGCGAG	130	0.0	42.461536	1
>>END_MODULE