FastQCFastQC Report
Sat 14 Jan 2017
SRR2933045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137704
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC193661.702200220795567No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156721.3775111979917447No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG95500.8394098992356537No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA22920.20145837581655685No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT22170.19486615147700984TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT21860.19214136541666374TruSeq Adapter, Index 23 (95% over 24bp)
GGGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT19700.17315575931876834TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT18730.16462981583962086TruSeq Adapter, Index 23 (95% over 24bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG17340.15241222673032703No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCG15870.1394914670248149No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14540.12780125586268484No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG14430.12683439629288462No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC14240.12516436612686604TruSeq Adapter, Index 22 (95% over 21bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCGTTCTACT13090.11505628880622727No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG11850.10415714456484287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTC206.311426E-446.00000445
AACCGTT206.311426E-446.00000437
TCGTTAA206.311426E-446.00000412
CGTATTA206.311426E-446.00000431
TCCGATC206.311426E-446.00000413
TCCGACC206.311426E-446.00000436
TTACTCG206.311426E-446.00000437
ATTCGAT206.311426E-446.00000414
TAACCGC206.311426E-446.00000426
GTTCGTA206.311426E-446.00000430
GTATCGG206.311426E-446.0000041
CCGGTAA206.311426E-446.00000417
CCAACCG206.311426E-446.00000413
GACGATA206.311426E-446.00000429
ACGAGCC206.311426E-446.00000439
TATCGTT206.311426E-446.00000427
TGTCGAT206.311426E-446.00000412
CCGTTGC206.311426E-446.00000422
TCGAACA206.311426E-446.00000428
GATCGTC551.8189894E-1246.09
Produced by FastQC (version 0.11.5)