##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137704
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42245522561229	33.0	31.0	34.0	31.0	34.0
2	32.813890959335644	34.0	31.0	34.0	31.0	34.0
3	32.86595986302237	34.0	31.0	34.0	31.0	34.0
4	36.32086992750311	37.0	37.0	37.0	35.0	37.0
5	36.17831351564203	37.0	37.0	37.0	35.0	37.0
6	36.26569125185461	37.0	37.0	37.0	35.0	37.0
7	36.4825015997131	37.0	37.0	37.0	35.0	37.0
8	36.428949006068365	37.0	37.0	37.0	35.0	37.0
9	38.25887225499778	39.0	39.0	39.0	37.0	39.0
10	37.96849356247319	39.0	38.0	39.0	35.0	39.0
11	37.649454515409985	39.0	37.0	39.0	35.0	39.0
12	37.21686132772672	39.0	35.0	39.0	35.0	39.0
13	37.07229824277668	39.0	35.0	39.0	34.0	39.0
14	37.82161265144537	40.0	36.0	41.0	32.0	41.0
15	38.128649455394374	40.0	36.0	41.0	33.0	41.0
16	38.37924099765844	40.0	36.0	41.0	34.0	41.0
17	38.45211232447104	40.0	36.0	41.0	35.0	41.0
18	38.413833475139405	40.0	36.0	41.0	35.0	41.0
19	38.34766687996175	40.0	36.0	41.0	35.0	41.0
20	38.1544443897534	40.0	35.0	41.0	34.0	41.0
21	38.11066586739609	40.0	35.0	41.0	34.0	41.0
22	38.05668697657739	40.0	35.0	41.0	34.0	41.0
23	38.03348234690218	40.0	35.0	41.0	34.0	41.0
24	37.97082896781588	40.0	35.0	41.0	34.0	41.0
25	37.81097719617757	40.0	35.0	41.0	34.0	41.0
26	37.71677694725518	40.0	35.0	41.0	34.0	41.0
27	37.591595880826645	40.0	35.0	41.0	34.0	41.0
28	37.577531589939035	39.0	35.0	41.0	34.0	41.0
29	37.52766624710821	40.0	35.0	41.0	34.0	41.0
30	37.48618885052703	40.0	35.0	41.0	34.0	41.0
31	37.36313135929908	40.0	35.0	41.0	33.0	41.0
32	37.24026196620562	40.0	35.0	41.0	33.0	41.0
33	37.13971032887289	39.0	35.0	41.0	33.0	41.0
34	36.97822983834108	39.0	35.0	41.0	33.0	41.0
35	36.904904087530674	39.0	35.0	41.0	33.0	41.0
36	36.821116916175036	39.0	35.0	41.0	32.0	41.0
37	36.703414948000535	39.0	35.0	41.0	32.0	41.0
38	36.68782917173535	39.0	35.0	41.0	32.0	41.0
39	36.67670852875616	39.0	35.0	41.0	32.0	41.0
40	36.60551514277879	39.0	35.0	41.0	32.0	41.0
41	36.43638327719688	39.0	35.0	41.0	31.0	41.0
42	36.45768495144607	39.0	35.0	41.0	31.0	41.0
43	36.399820164120015	39.0	35.0	41.0	31.0	41.0
44	36.36107282737865	38.0	35.0	41.0	31.0	41.0
45	36.30486400680669	38.0	35.0	41.0	31.0	41.0
46	36.24965984122408	38.0	35.0	41.0	31.0	41.0
47	36.15080460295472	38.0	35.0	41.0	31.0	41.0
48	36.02548905514967	38.0	35.0	41.0	31.0	41.0
49	36.00812074142308	38.0	35.0	41.0	31.0	41.0
50	35.90270228460127	37.0	35.0	41.0	31.0	41.0
51	35.822705202759245	37.0	35.0	40.0	31.0	41.0
52	35.585204060107024	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	8.0
14	8.0
15	23.0
16	54.0
17	153.0
18	294.0
19	473.0
20	874.0
21	1428.0
22	2158.0
23	3209.0
24	4952.0
25	7545.0
26	9984.0
27	11552.0
28	12792.0
29	13741.0
30	15830.0
31	18951.0
32	24243.0
33	34256.0
34	89819.0
35	130112.0
36	76712.0
37	85587.0
38	134749.0
39	455101.0
40	3093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.205543796980585	22.29138686336692	26.02979333816177	13.473276001490722
2	37.72976099231434	22.959047344476243	26.02328901014675	13.287902653062659
3	33.854412043905974	23.378928086743127	29.541778880974313	13.224880988376588
4	30.293292455682675	27.24777270713648	28.226058799125255	14.232876038055592
5	25.959827863838047	30.113280783050776	28.668880482093762	15.258010871017417
6	27.242411031340314	37.98299030327748	25.543023492929617	9.231575172452589
7	86.1616026664229	1.881772411804828	9.268843214052161	2.6877817077201103
8	83.96876516211597	2.2688678250230288	9.71931187725454	4.0430551356064495
9	78.26121732893617	3.976341825290234	12.644765246496453	5.11767559927714
10	48.871235400420495	20.42736950911661	17.780108007003577	12.921287083459317
11	33.08690133813364	23.601921062068868	27.135177515417013	16.17600008438047
12	32.831650411706384	18.12923220802599	30.625804251369427	18.413313128898203
13	28.613857382939674	21.00335412374396	33.9781700688404	16.404618424475963
14	18.78854253830522	22.765675430516197	37.88648013894651	20.559301892232074
15	15.933757813983252	28.13411924366971	38.041529255412655	17.890593686934388
16	26.86929113372195	25.332423899362222	31.453611835767475	16.344673131148348
17	25.800471827470062	24.645514123181425	26.727250673285845	22.82676337606267
18	22.86658041107353	23.628553648400636	33.678355705877806	19.82651023464803
19	22.981988285177867	26.446597709070197	27.438068249738066	23.133345756013867
20	18.480993298784217	35.486822583026864	26.636102184751042	19.396081933437873
21	23.700716530837546	28.115485222869918	32.150278103970805	16.033520142321727
22	20.951143706974747	23.220626806269472	34.36113435480582	21.46709513194996
23	21.770425347893653	24.76259202745178	32.96164907568225	20.505333548972317
24	22.196195143903864	28.19344926272563	29.889760429777866	19.720595163592638
25	23.476756695942	25.116286837349612	30.17410503962366	21.232851427084725
26	19.37340468170983	29.138422647718563	26.255862684845972	25.23230998572564
27	22.939885945729294	25.90893589193674	30.48147848649561	20.669699675838356
28	17.935508708767834	28.916220739313562	27.86963920316708	25.278631348751517
29	21.699141428702017	27.039985795953957	27.2169210972274	24.043951678116628
30	26.4247115242629	23.820870806466356	26.28003417409098	23.474383495179765
31	23.65448306413619	24.54091749699395	30.28590916442238	21.518690274447483
32	25.949368201219297	23.90270228460127	25.778234057364656	24.369695456814778
33	25.318360487437857	23.543030524635583	28.506887555989955	22.6317214319366
34	18.579173493281203	22.65158600127977	35.192106206886855	23.57713429855217
35	21.145921962127233	26.93231279840802	29.42461308037943	22.497152159085314
36	25.243912300563238	24.991825641818963	25.391226540470985	24.373035517146814
37	19.319875820072706	28.107222968364354	29.754487986330364	22.81841322523257
38	21.589095230393845	25.63417198146442	31.56233958920774	21.214393198933994
39	19.75830268681485	26.81839916182065	26.673370226350613	26.74992792501389
40	25.876414251861647	23.757233867508596	29.483239928839133	20.883111951790625
41	18.809989241489877	25.84644160519784	26.673282330026087	28.670286823286194
42	18.69589981225345	26.207783395329542	30.71457953914199	24.38173725327502
43	19.965034842103044	26.2810889299853	30.944164738807284	22.809711489104373
44	23.48009675627404	24.502682595824574	27.975730066871524	24.041490581029866
45	19.739229184392425	25.062933768361546	26.395354151870787	28.80248289537525
46	22.688941939203872	24.654391651958683	28.169014084507044	24.487652324330405
47	19.166145148474474	23.418481432780407	32.1377089295634	25.277664489181724
48	18.681133229732865	28.768203328809605	29.1781517864049	23.372511655052634
49	23.643320230921226	24.895579166461577	29.246886712185244	22.214213890431957
50	21.07876917018838	25.09519171946306	29.187029315182155	24.639009795166405
51	20.01583891767982	25.615977442287274	27.43859562768523	26.929588012347676
52	20.183457208553367	26.236173908151855	29.39384936679488	24.1865195164999
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1053.0
1	2363.5
2	3674.0
3	11332.0
4	18990.0
5	11178.5
6	3367.0
7	3926.5
8	4486.0
9	5911.5
10	7337.0
11	8845.0
12	10353.0
13	10891.5
14	10435.0
15	9440.0
16	8305.5
17	7171.0
18	6456.5
19	5742.0
20	5767.5
21	5793.0
22	5971.5
23	6150.0
24	6312.5
25	6475.0
26	6524.5
27	6574.0
28	7857.0
29	9140.0
30	10055.0
31	10970.0
32	13024.0
33	15078.0
34	17637.0
35	20196.0
36	19460.5
37	18725.0
38	23934.5
39	30536.5
40	31929.0
41	35464.0
42	38999.0
43	46920.5
44	54842.0
45	64250.5
46	73659.0
47	85889.5
48	98120.0
49	108369.5
50	118619.0
51	107904.5
52	97190.0
53	87760.5
54	78331.0
55	76232.5
56	74134.0
57	67936.0
58	61738.0
59	65135.5
60	68533.0
61	52280.0
62	36027.0
63	32286.5
64	23839.0
65	19132.0
66	17322.5
67	15513.0
68	13156.0
69	10799.0
70	9165.5
71	7532.0
72	6354.5
73	5177.0
74	4049.0
75	2921.0
76	2471.5
77	2022.0
78	1658.0
79	1294.0
80	866.0
81	438.0
82	431.0
83	424.0
84	353.5
85	283.0
86	235.0
87	187.0
88	100.5
89	11.5
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1137704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.305882911348323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.78356998476575	15.081064541426487
2	11.675475975769261	4.975126481480005
3	4.771135715930367	3.0495977675329335
4	2.4501142467423875	2.0880738904207883
5	1.503333394642082	1.6014922641482001
6	0.9836306820679765	1.257427208409
7	0.7666253768018999	1.1433541360506698
8	0.5365980096287992	0.9146155490891006
9	0.44637975781537587	0.8559463368609379
>10	3.9495754248457513	18.623783782901583
>50	1.3315110995200472	20.421111332957885
>100	0.7867456062179405	22.86327936881559
>500	0.008272824460756474	1.1931909144938433
>1k	0.005790977122529532	2.0023997908423525
>5k	4.136412230378237E-4	0.8416406849410002
>10k+	8.272824460756474E-4	3.0878959496296092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	19366	1.702200220795567	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15672	1.3775111979917447	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	9550	0.8394098992356537	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	2292	0.20145837581655685	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT	2217	0.19486615147700984	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	2186	0.19214136541666374	TruSeq Adapter, Index 23 (95% over 24bp)
GGGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1970	0.17315575931876834	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	1873	0.16462981583962086	TruSeq Adapter, Index 23 (95% over 24bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1734	0.15241222673032703	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCG	1587	0.1394914670248149	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1454	0.12780125586268484	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1443	0.12683439629288462	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1424	0.12516436612686604	TruSeq Adapter, Index 22 (95% over 21bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCGTTCTACT	1309	0.11505628880622727	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	1185	0.10415714456484287	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20005203462412016	0.0
2	0.0	0.0	0.0	0.686821879856272	0.0
3	0.0	0.0	0.0	1.1022199095722613	0.0
4	0.0	0.0	0.0	1.592241918811923	0.0
5	0.0	0.0	0.0	3.0450802669235584	0.0
6	0.0	0.0	0.0	4.4539704527715465	0.0
7	0.0	0.0	0.0	5.610598187226203	0.0
8	0.0	0.0	0.0	7.606899509890094	0.0
9	0.0	0.0	0.0	8.648031473915887	0.0
10	0.0	0.0	0.0	9.702435782945301	0.0
11	0.0	0.0	0.0	11.330012024217195	0.0
12	0.0	0.0	0.0	12.239035812478466	0.0
13	0.0	0.0	0.0	12.655137012790673	0.0
14	0.0	0.0	0.0	12.923660284221555	0.0
15	0.0	0.0	0.0	13.198160505720292	0.0
16	0.0	0.0	0.0	13.685018247276972	0.0
17	0.0	0.0	0.0	14.219515796727444	0.0
18	0.0	0.0	0.0	14.828461533052534	0.0
19	0.0	0.0	0.0	15.183914269440908	0.0
20	0.0	0.0	0.0	15.675342619873007	0.0
21	0.0	0.0	0.0	16.132579300063988	0.0
22	0.0	0.0	0.0	16.608450001054756	0.0
23	0.0	0.0	0.0	17.03668089415173	0.0
24	8.789632452729356E-5	0.0	0.0	17.343526963076513	0.0
25	8.789632452729356E-5	0.0	0.0	17.716295275396764	0.0
26	1.7579264905458712E-4	0.0	0.0	18.031930976774277	0.0
27	1.7579264905458712E-4	0.0	0.0	18.47281894060318	0.0
28	1.7579264905458712E-4	0.0	0.0	18.814911435663408	0.0
29	1.7579264905458712E-4	0.0	0.0	19.238044341937798	0.0
30	1.7579264905458712E-4	0.0	0.0	20.419986217856316	0.0
31	1.7579264905458712E-4	0.0	0.0	20.92969700379009	0.0
32	1.7579264905458712E-4	0.0	0.0	21.260714561959876	0.0
33	1.7579264905458712E-4	0.0	0.0	21.65484168114026	0.0
34	1.7579264905458712E-4	0.0	0.0	22.077535105792016	0.0
35	1.7579264905458712E-4	0.0	0.0	22.511215571009682	0.0
36	1.7579264905458712E-4	0.0	0.0	22.808568836885517	0.0
37	1.7579264905458712E-4	0.0	0.0	23.162703128405983	0.0
38	1.7579264905458712E-4	0.0	0.0	23.6261804476384	0.0
39	1.7579264905458712E-4	0.0	0.0	25.171749418126332	0.0
40	1.7579264905458712E-4	0.0	0.0	25.570007664559498	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTC	20	6.311426E-4	46.000004	45
AACCGTT	20	6.311426E-4	46.000004	37
TCGTTAA	20	6.311426E-4	46.000004	12
CGTATTA	20	6.311426E-4	46.000004	31
TCCGATC	20	6.311426E-4	46.000004	13
TCCGACC	20	6.311426E-4	46.000004	36
TTACTCG	20	6.311426E-4	46.000004	37
ATTCGAT	20	6.311426E-4	46.000004	14
TAACCGC	20	6.311426E-4	46.000004	26
GTTCGTA	20	6.311426E-4	46.000004	30
GTATCGG	20	6.311426E-4	46.000004	1
CCGGTAA	20	6.311426E-4	46.000004	17
CCAACCG	20	6.311426E-4	46.000004	13
GACGATA	20	6.311426E-4	46.000004	29
ACGAGCC	20	6.311426E-4	46.000004	39
TATCGTT	20	6.311426E-4	46.000004	27
TGTCGAT	20	6.311426E-4	46.000004	12
CCGTTGC	20	6.311426E-4	46.000004	22
TCGAACA	20	6.311426E-4	46.000004	28
GATCGTC	55	1.8189894E-12	46.0	9
>>END_MODULE