FastQCFastQC Report
Sat 14 Jan 2017
SRR2933044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050008
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC67270.6406617854340158No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58770.5597100212569809No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34800.33142604627774264No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30410.28961684101454466No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21410.20390320835650777No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15120.143998902865502No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT14840.14133225651614084Illumina Single End Adapter 1 (95% over 21bp)
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTA12860.12247525733137271No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12520.11923718676429132No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT12380.11790386358961076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA206.311203E-446.00000436
CCAATCG206.311203E-446.00000423
TACGGGT700.046.0000043
CGTAGAT206.311203E-446.00000422
CGTAATC206.311203E-446.00000410
ACGTACT206.311203E-446.00000429
ACGTACA351.0194526E-746.00000428
CACGTAC206.311203E-446.00000428
CGAGTGA253.4169527E-546.015
ATCCGAT253.4169527E-546.039
TACGCGG1300.046.01
ATGCGTA253.4169527E-546.019
TTACGCT253.4169527E-546.035
GCGTACC253.4169527E-546.021
TCACGAC1950.043.64102624
CGGTCTA1750.043.37143330
CTAACGG1500.042.933331
ATAACGG750.042.933331
AATGCGG1400.042.7142871
TACACGG700.042.7142871
Produced by FastQC (version 0.11.5)