##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933044.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050008
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58352793502526	33.0	31.0	34.0	31.0	34.0
2	32.963324088959325	34.0	31.0	34.0	31.0	34.0
3	33.06424617717198	34.0	33.0	34.0	31.0	34.0
4	36.460097446876595	37.0	37.0	37.0	35.0	37.0
5	36.44439185225255	37.0	37.0	37.0	35.0	37.0
6	36.55201484179168	37.0	37.0	37.0	35.0	37.0
7	36.57284420690128	37.0	37.0	37.0	35.0	37.0
8	36.45005657099755	37.0	37.0	37.0	35.0	37.0
9	38.1478855399197	39.0	39.0	39.0	37.0	39.0
10	37.99981238238185	39.0	38.0	39.0	35.0	39.0
11	37.498114300081525	39.0	37.0	39.0	35.0	39.0
12	37.3415640642738	39.0	37.0	39.0	35.0	39.0
13	37.35151636939909	39.0	37.0	39.0	35.0	39.0
14	38.73854008731362	41.0	37.0	41.0	35.0	41.0
15	38.79666250161903	41.0	37.0	41.0	35.0	41.0
16	38.769811277628364	41.0	37.0	41.0	35.0	41.0
17	38.77467219297377	41.0	37.0	41.0	35.0	41.0
18	38.705287007337084	40.0	37.0	41.0	35.0	41.0
19	38.59422975824946	40.0	36.0	41.0	35.0	41.0
20	38.46401646463646	40.0	35.0	41.0	35.0	41.0
21	38.40436930004343	40.0	35.0	41.0	35.0	41.0
22	38.36546007268516	40.0	35.0	41.0	35.0	41.0
23	38.31437284287358	40.0	35.0	41.0	35.0	41.0
24	38.26016658920694	40.0	35.0	41.0	35.0	41.0
25	38.19558898598868	40.0	35.0	41.0	35.0	41.0
26	38.10307826226086	40.0	35.0	41.0	35.0	41.0
27	38.040245407654034	40.0	35.0	41.0	35.0	41.0
28	38.0586281247381	40.0	35.0	41.0	35.0	41.0
29	38.019747468590715	40.0	35.0	41.0	35.0	41.0
30	37.9329252729503	40.0	35.0	41.0	34.0	41.0
31	37.845369749563815	40.0	35.0	41.0	34.0	41.0
32	37.73528582639371	40.0	35.0	41.0	34.0	41.0
33	37.59341357399182	40.0	35.0	41.0	34.0	41.0
34	37.469406899756954	40.0	35.0	41.0	34.0	41.0
35	37.32313277613123	40.0	35.0	41.0	33.0	41.0
36	37.23709533641649	40.0	35.0	41.0	33.0	41.0
37	37.13035138779895	39.0	35.0	41.0	33.0	41.0
38	37.120720984982974	39.0	35.0	41.0	33.0	41.0
39	37.01698653724543	39.0	35.0	41.0	33.0	41.0
40	36.996949547051074	39.0	35.0	41.0	33.0	41.0
41	36.941720444034715	39.0	35.0	41.0	33.0	41.0
42	36.859708687933804	39.0	35.0	41.0	33.0	41.0
43	36.75777708360317	39.0	35.0	41.0	33.0	41.0
44	36.64790649214101	38.0	35.0	41.0	33.0	41.0
45	36.58694505184722	38.0	35.0	41.0	33.0	41.0
46	36.48962674570099	38.0	35.0	41.0	33.0	41.0
47	36.41131019954134	38.0	35.0	41.0	33.0	41.0
48	36.33034034026407	37.0	35.0	41.0	32.0	41.0
49	36.23787056860519	37.0	35.0	40.0	32.0	41.0
50	36.12419524422671	37.0	35.0	40.0	32.0	41.0
51	36.0101513512278	37.0	35.0	40.0	31.0	41.0
52	35.76330085104114	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	6.0
14	9.0
15	12.0
16	33.0
17	63.0
18	151.0
19	293.0
20	462.0
21	818.0
22	1339.0
23	2090.0
24	3376.0
25	5034.0
26	7241.0
27	8700.0
28	9051.0
29	9656.0
30	10955.0
31	13478.0
32	17214.0
33	24212.0
34	63177.0
35	149277.0
36	61048.0
37	85403.0
38	140322.0
39	431854.0
40	4728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.4704992723865	21.93002339029798	23.48563058567173	20.113846751643795
2	27.391505588528847	24.088102185888108	26.775891231304904	21.744500994278138
3	28.26864176272943	24.07514990361978	26.060468110719153	21.595740222931635
4	24.52771788405422	27.259792306344334	24.0220074513718	24.19048235822965
5	22.424400575995612	37.35123922865349	21.636025630280912	18.58833456506998
6	88.32427943406145	3.3880694242329583	6.284809258596125	2.002841883109462
7	90.31807376705702	2.387981805852908	5.060532872130498	2.233411554959581
8	84.60040304454823	4.096444979466823	7.539085416492066	3.7640665594928797
9	46.92469009759926	24.74524003626639	13.467421200600377	14.862648665533976
10	37.579999390480836	22.831826043230148	21.964785030209296	17.623389536079724
11	32.779083587934565	20.367368629572347	27.988262946568028	18.86528483592506
12	22.615827688931894	28.751780938811894	29.042445390892258	19.58994598136395
13	19.363757228516356	32.48584772687446	29.326824176577702	18.82357086803148
14	17.814245224798285	24.93257194230901	37.45314321414694	19.800039618745764
15	20.432891939870935	19.211758386602767	38.11894766516065	22.236402008365648
16	20.70222322115641	21.14945790889212	28.190451882271372	29.95786698768009
17	23.061633816123305	23.58401078848923	30.7548132966606	22.599542098726864
18	26.726843985950584	26.30627576170848	25.41933013843704	21.547550113903892
19	28.32083184128121	22.64830363197233	25.451139419890133	23.579725106856326
20	25.340949783239747	25.95208798409155	27.34541070163275	21.361551531035953
21	22.832587942187107	22.619732421086315	26.323704200348953	28.22397543637763
22	21.033363555325295	27.986834386023723	25.038666372065737	25.94113568658524
23	20.612128669495853	25.412568285194016	32.651846462122194	21.323456583187937
24	24.07553085309826	23.52039222558304	29.780344530708337	22.623732390610357
25	20.117465771689357	28.011977051603417	26.26037134955162	25.6101858271556
26	19.853848732581085	26.615035314016655	29.431394808420507	24.099721144981753
27	21.227362077241317	24.23505344721183	33.72840968830714	20.80917478723972
28	21.178981493474335	25.126665701594654	29.982819178520543	23.711533626410468
29	21.880785670204418	25.53713876465703	30.700432758607555	21.881642806530998
30	29.964247891444636	24.524384576117516	24.189339509794213	21.32202802264364
31	28.50483043938713	25.785041637778	25.204665107313467	20.505462815521405
32	26.884461832671754	24.60124113340089	24.892096060220496	23.622200973706867
33	23.46305932907178	24.378861875338092	26.726177324363242	25.431901471226887
34	24.086197438495706	23.256298999626672	27.853311593816425	24.804191968061197
35	29.8880579957486	23.925722470685937	25.221712596475456	20.964506937090004
36	24.032578799399623	26.577987977234457	26.937128098071632	22.452305125294284
37	24.802477695408033	28.17930911002583	24.60505062818569	22.413162566380446
38	23.008586601244943	26.29951390846546	25.057047184402403	25.634852305887197
39	24.46105172532019	23.25667994910515	28.66511493245766	23.617153393117004
40	21.040506358046795	22.428781494998134	30.04310443349003	26.48760771346504
41	23.035634014217035	25.960373635248494	24.456099382099946	26.547892968434528
42	22.84163549230101	23.71667644436995	27.185030971192603	26.256657092136443
43	23.55743956236524	25.196760405635004	28.91977965882165	22.32602037317811
44	22.95296797738684	27.708741266733206	26.13865799117721	23.199632764702745
45	25.81037477809693	25.499805715765977	25.992468628810446	22.69735087732665
46	25.194950895612223	24.845144036997812	26.58332126993318	23.37658379745678
47	23.5087732664894	25.141522731255385	29.19958705076533	22.150116951489892
48	22.979063016662728	24.3541953966065	28.562544285376873	24.104197301353896
49	21.762405619766707	25.67028060738585	29.7858682981463	22.781445474701144
50	21.668406335951726	26.635511348484965	28.314546174886285	23.381536140677024
51	23.017824626098086	22.137545618700045	29.20739651507417	25.637233240127692
52	24.16181590997402	22.76658844504042	27.757978986826764	25.313616658158793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1437.0
1	1700.5
2	1964.0
3	5384.0
4	8804.0
5	5715.0
6	2626.0
7	2769.0
8	2912.0
9	3086.0
10	3260.0
11	3423.0
12	3586.0
13	3440.5
14	3294.5
15	3294.0
16	3072.5
17	2851.0
18	2613.0
19	2375.0
20	2374.5
21	2374.0
22	2712.5
23	3051.0
24	3332.5
25	3614.0
26	3822.5
27	4031.0
28	4642.0
29	5253.0
30	6852.0
31	8451.0
32	11206.5
33	13962.0
34	15634.5
35	17307.0
36	18635.5
37	19964.0
38	22110.0
39	26527.5
40	28799.0
41	36987.0
42	45175.0
43	54553.5
44	63932.0
45	67843.5
46	71755.0
47	74898.0
48	78041.0
49	86601.0
50	95161.0
51	95472.5
52	95784.0
53	86864.0
54	77944.0
55	73963.5
56	69983.0
57	65193.0
58	60403.0
59	57917.5
60	55432.0
61	50372.5
62	45313.0
63	41135.0
64	32065.0
65	27173.0
66	22684.5
67	18196.0
68	15412.5
69	12629.0
70	11491.0
71	10353.0
72	9435.5
73	8518.0
74	6533.5
75	4549.0
76	3646.5
77	2744.0
78	2002.5
79	1261.0
80	976.0
81	691.0
82	528.0
83	365.0
84	238.0
85	111.0
86	70.0
87	29.0
88	30.0
89	19.5
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1050008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.61554609176915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9832329997037	15.458202960152576
2	9.37047891634235	3.8635508000361356
3	3.1736404957500337	1.962789957565198
4	1.5513465032079492	1.279274213647535
5	0.975496019850626	1.0055191579783969
6	0.6808774352335574	0.8421996089341781
7	0.5026027049879849	0.7253000461369358
8	0.4194717214546886	0.6918110886274307
9	0.35865490077259043	0.6654479974124607
>10	5.377194508794556	27.80142355695257
>50	2.2284855604584224	32.638962376378856
>100	0.37017878643989466	9.86757328549493
>500	0.0037064208905120866	0.5199693062396856
>1k	0.0037064208905120866	1.4741378164039873
>5k	9.266052226280216E-4	1.203837828039125
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6727	0.6406617854340158	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5877	0.5597100212569809	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3480	0.33142604627774264	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3041	0.28961684101454466	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2141	0.20390320835650777	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1512	0.143998902865502	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT	1484	0.14133225651614084	Illumina Single End Adapter 1 (95% over 21bp)
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTA	1286	0.12247525733137271	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1252	0.11923718676429132	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	1238	0.11790386358961076	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14447508971360218	0.0
2	0.0	0.0	0.0	0.40228264927505314	0.0
3	0.0	0.0	0.0	0.6762805616719111	0.0
4	0.0	0.0	0.0	0.8789456842233583	0.0
5	0.0	0.0	0.0	1.884652307410991	0.0
6	0.0	0.0	0.0	2.1082696512788472	0.0
7	0.0	0.0	0.0	2.799121530502625	0.0
8	9.523736962004099E-5	0.0	0.0	3.643115100075428	0.0
9	9.523736962004099E-5	0.0	0.0	4.458537458762219	0.0
10	9.523736962004099E-5	0.0	0.0	4.985581062239525	0.0
11	9.523736962004099E-5	0.0	0.0	5.492529580727004	0.0
12	9.523736962004099E-5	0.0	0.0	5.910431158619744	0.0
13	9.523736962004099E-5	0.0	0.0	6.068810904297872	0.0
14	9.523736962004099E-5	0.0	0.0	6.178429116730539	0.0
15	1.9047473924008198E-4	0.0	0.0	6.521378884732306	0.0
16	1.9047473924008198E-4	0.0	0.0	6.939756649473147	0.0
17	2.85712108860123E-4	0.0	0.0	7.48232394419852	0.0
18	2.85712108860123E-4	0.0	0.0	7.750988563896656	0.0
19	2.85712108860123E-4	0.0	0.0	8.028224546860596	0.0
20	2.85712108860123E-4	0.0	0.0	8.373650486472483	0.0
21	2.85712108860123E-4	0.0	0.0	8.696314694745183	0.0
22	4.7618684810020494E-4	0.0	0.0	9.045550129141873	0.0
23	4.7618684810020494E-4	0.0	0.0	9.322881349475432	0.0
24	4.7618684810020494E-4	0.0	0.0	9.551736748672392	0.0
25	5.71424217720246E-4	0.0	0.0	9.768401764557984	0.0
26	5.71424217720246E-4	0.0	0.0	9.994590517405582	0.0
27	6.666615873402869E-4	0.0	0.0	10.243921951070849	0.0
28	6.666615873402869E-4	0.0	0.0	10.506110429634822	0.0
29	6.666615873402869E-4	0.0	0.0	10.770108418221575	0.0
30	6.666615873402869E-4	0.0	0.0	11.026106467760245	0.0
31	7.618989569603279E-4	0.0	0.0	11.248104776344562	0.0
32	7.618989569603279E-4	0.0	0.0	11.520007466609778	0.0
33	7.618989569603279E-4	0.0	0.0	11.78029119778135	0.0
34	7.618989569603279E-4	0.0	0.0	12.114669602517314	0.0
35	7.618989569603279E-4	0.0	0.0	12.346667834911734	0.0
36	8.57136326580369E-4	0.0	0.0	12.599332576513703	0.0
37	9.523736962004099E-4	0.0	0.0	12.865806736710578	0.0
38	9.523736962004099E-4	0.0	0.0	13.184375738089615	0.0
39	9.523736962004099E-4	0.0	0.0	13.484468689762364	0.0
40	9.523736962004099E-4	0.0	0.0	13.75389520841746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	20	6.311203E-4	46.000004	36
CCAATCG	20	6.311203E-4	46.000004	23
TACGGGT	70	0.0	46.000004	3
CGTAGAT	20	6.311203E-4	46.000004	22
CGTAATC	20	6.311203E-4	46.000004	10
ACGTACT	20	6.311203E-4	46.000004	29
ACGTACA	35	1.0194526E-7	46.000004	28
CACGTAC	20	6.311203E-4	46.000004	28
CGAGTGA	25	3.4169527E-5	46.0	15
ATCCGAT	25	3.4169527E-5	46.0	39
TACGCGG	130	0.0	46.0	1
ATGCGTA	25	3.4169527E-5	46.0	19
TTACGCT	25	3.4169527E-5	46.0	35
GCGTACC	25	3.4169527E-5	46.0	21
TCACGAC	195	0.0	43.641026	24
CGGTCTA	175	0.0	43.371433	30
CTAACGG	150	0.0	42.93333	1
ATAACGG	75	0.0	42.93333	1
AATGCGG	140	0.0	42.714287	1
TACACGG	70	0.0	42.714287	1
>>END_MODULE