FastQCFastQC Report
Sat 14 Jan 2017
SRR2933043.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933043.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123452
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233222.0759231369030453No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC106950.9519765864496214No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC87090.7752000085450914No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC78930.7025667318229885No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39930.3554223945482317No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30280.2695264239148624No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT28710.2555516390553402Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT26250.23365484239647089No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC23610.21015584110402583No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT20010.17811174843250982No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC19680.17517437327095417No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG18810.16743038420867112No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT17830.1587072700925362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17680.1573720995645564No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT17180.15292153113795695No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC16480.14669073534071772No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC13610.12114447257203691No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA12230.10886090371462244No Hit
GGACAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12090.10761474455517459No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA11830.10530044897334287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGG551.8189894E-1246.0000041
CCTACGG551.8189894E-1246.0000041
ACTACGG700.046.0000041
GCGATCG351.01954356E-746.0000048
TGGGCGC1400.046.0000045
GCTACGG2200.046.0000041
GTTATCT2150.046.00000424
ATTTAGG1600.046.01
TCGCCCA301.8613646E-646.045
CGGGTAC1000.046.05
GCGACTA206.311393E-446.028
CCCTCGC206.311393E-446.042
CGACGCG206.311393E-446.046
GCAACCG206.311393E-446.08
GGCGTGC206.311393E-446.019
CGGCACT301.8613646E-646.039
TCCCTCG206.311393E-446.041
ATTCGGC800.046.016
CCGTCGG253.4171055E-546.034
GTGGACG206.311393E-446.012
Produced by FastQC (version 0.11.5)