##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933043.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123452
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42948519384896	33.0	31.0	34.0	31.0	34.0
2	32.7467377333433	34.0	31.0	34.0	31.0	34.0
3	32.78417769517523	34.0	31.0	34.0	31.0	34.0
4	36.261431730060565	37.0	37.0	37.0	35.0	37.0
5	36.23329345624023	37.0	37.0	37.0	35.0	37.0
6	36.406558535656174	37.0	37.0	37.0	35.0	37.0
7	36.49587521318223	37.0	37.0	37.0	35.0	37.0
8	36.32901094127742	37.0	37.0	37.0	35.0	37.0
9	37.965697688908826	39.0	38.0	39.0	35.0	39.0
10	37.83656177566999	39.0	38.0	39.0	35.0	39.0
11	37.3152666958624	39.0	35.0	39.0	35.0	39.0
12	37.106658762457144	39.0	35.0	39.0	34.0	39.0
13	36.756799578442156	39.0	35.0	39.0	33.0	39.0
14	38.291415209550564	40.0	36.0	41.0	33.0	41.0
15	38.48079935769397	40.0	36.0	41.0	35.0	41.0
16	38.53213399415373	40.0	36.0	41.0	35.0	41.0
17	38.56251446434739	40.0	36.0	41.0	35.0	41.0
18	38.45193831156115	40.0	36.0	41.0	35.0	41.0
19	38.28443938859871	40.0	36.0	41.0	34.0	41.0
20	38.16808016719895	40.0	35.0	41.0	34.0	41.0
21	38.166319522329395	40.0	35.0	41.0	34.0	41.0
22	38.141551218921684	40.0	35.0	41.0	34.0	41.0
23	38.07201998839292	40.0	35.0	41.0	34.0	41.0
24	37.99657573265257	40.0	35.0	41.0	34.0	41.0
25	37.91464432837362	40.0	35.0	41.0	34.0	41.0
26	37.81504772789581	40.0	35.0	41.0	34.0	41.0
27	37.74718902098176	40.0	35.0	41.0	34.0	41.0
28	37.71016207189982	40.0	35.0	41.0	34.0	41.0
29	37.64155477937642	40.0	35.0	41.0	34.0	41.0
30	37.52206324791803	40.0	35.0	41.0	34.0	41.0
31	37.46943260593243	39.0	35.0	41.0	34.0	41.0
32	37.395327971288495	40.0	35.0	41.0	34.0	41.0
33	37.20567500881212	39.0	35.0	41.0	33.0	41.0
34	37.09182323766392	39.0	35.0	41.0	33.0	41.0
35	36.92623716901122	39.0	35.0	41.0	33.0	41.0
36	36.84729476648758	39.0	35.0	41.0	33.0	41.0
37	36.71117769161477	39.0	35.0	41.0	32.0	41.0
38	36.691825729982234	39.0	35.0	41.0	32.0	41.0
39	36.585839893471196	39.0	35.0	41.0	32.0	41.0
40	36.498374652410604	39.0	35.0	41.0	32.0	41.0
41	36.501109971765594	38.0	35.0	41.0	32.0	41.0
42	36.49274735369201	38.0	35.0	41.0	32.0	41.0
43	36.400893852162795	38.0	35.0	41.0	32.0	41.0
44	36.31742433143561	38.0	35.0	41.0	32.0	41.0
45	36.293020084525196	38.0	35.0	41.0	32.0	41.0
46	36.219213637965844	38.0	35.0	41.0	32.0	41.0
47	36.08589953108811	37.0	35.0	41.0	31.0	41.0
48	36.05404058206314	37.0	35.0	41.0	31.0	41.0
49	35.96367535061578	37.0	35.0	40.0	31.0	41.0
50	35.85709224782189	37.0	35.0	40.0	31.0	41.0
51	35.74385376500287	37.0	35.0	40.0	31.0	41.0
52	35.49964128418482	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	5.0
14	12.0
15	17.0
16	39.0
17	103.0
18	213.0
19	410.0
20	699.0
21	1185.0
22	1780.0
23	2847.0
24	4601.0
25	7566.0
26	9975.0
27	10815.0
28	11042.0
29	12288.0
30	14723.0
31	18193.0
32	23324.0
33	32326.0
34	82727.0
35	144616.0
36	92490.0
37	89922.0
38	122812.0
39	431006.0
40	7711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.44971747791628	23.526861850795584	24.966531725431974	19.056888945856166
2	30.105692098994886	23.60563691194639	26.079885923030087	20.208785066028632
3	27.048151589921066	24.226491207457016	27.87391005579233	20.851447146829592
4	22.781836696182836	26.721568878777198	27.500952421643294	22.995642003396675
5	21.102548217458335	37.38753413585984	23.32320383959439	18.18671380708744
6	84.11120368293439	2.6265474626419283	10.645047585477617	2.61720126894607
7	86.202614797962	1.7123116964498708	8.119617037488029	3.965456468100106
8	77.72072149054877	3.997767594877218	12.82689425093373	5.45461666364028
9	40.35250282166038	28.10596269355522	17.057604597259164	14.483929887525235
10	34.19807877862161	19.311105414383526	28.335434001630688	18.155381805364183
11	32.165504178193636	19.06463293491845	30.968924351018114	17.8009385358698
12	22.631852540206435	27.28590095527001	33.858767441777665	16.223479062745895
13	15.107454524091818	34.084055215532125	34.17351164090678	16.63497861946928
14	12.724175131647813	29.473978416523362	40.51744088755016	17.28440556427867
15	17.499546042020487	21.640799962971272	42.214531639981054	18.645122355027183
16	17.536663782698326	25.51208240316453	29.029722676180203	27.92153113795694
17	20.139979278153405	30.110765747001206	30.65774060663028	19.09151436821511
18	22.13036248989721	35.36751013839488	26.032798909076664	16.469328462631246
19	18.990397453562768	30.93670223560953	25.605010271911926	24.467890038915773
20	18.20549520584769	31.72320668795819	30.35973054478518	19.711567561408945
21	14.535378458536725	27.95339720789139	30.446605640472402	27.064618693099483
22	12.047599719436167	34.761431730060565	28.108900068716775	25.082068481786497
23	13.035625910141244	36.6787366082396	32.05958064964057	18.22605683197858
24	22.82135774381104	28.575675685298524	27.94672135525149	20.65624521563894
25	13.754481722405584	32.148770040909625	25.961945859725205	28.134802376959584
26	12.74420268956751	31.12531732552882	33.471835022769106	22.658644962134563
27	16.573204729708078	29.246910415398254	33.20880642875708	20.971078426136586
28	14.421354895447248	35.12700142062144	27.280382250421027	23.17126143351029
29	18.952745644673737	31.678968037797787	30.88881411933932	18.479472198189153
30	24.58209162474231	28.346293388591594	26.08611671882733	20.985498267838768
31	23.183634013736235	31.69303183402584	25.64853683112407	19.47479732111385
32	21.41613526879653	29.45973659755824	24.370956658584433	24.753171475060796
33	14.42642854345357	27.437220281774387	31.151486667877222	26.98486450689482
34	16.60711806111877	29.165910070034144	29.294976554405526	24.93199531444156
35	25.948861188551003	27.18104556313932	27.67123116964499	19.198862078664686
36	19.724563221214613	31.537350950463395	27.621295791898543	21.116790036423453
37	20.46638396656021	30.93937257666549	28.60229008448959	19.99195337228471
38	18.40461363725375	27.259642601553068	25.970579962472804	28.36516379872037
39	25.32676073388093	25.177132623378657	29.589693195614945	19.906413447125466
40	18.171759897174066	23.71378572471276	32.93207008399113	25.182384294122045
41	18.245639333055617	31.231329865450412	26.074456229549636	24.448574571944327
42	18.818516500927498	25.242644990618203	29.329690988133006	26.609147520321297
43	20.978644392461806	27.995143539732897	31.578563214093702	19.4476488537116
44	18.795996624688904	31.734778165867343	25.67052263915147	23.798702570292278
45	21.44203757703934	28.677682713636187	27.27290529546434	22.607374413860136
46	23.623706219758386	27.419507019436523	27.91930585374364	21.03748090706145
47	17.64240928851433	31.441218672448844	29.169114479301296	21.74725755973553
48	20.04064259087171	27.44710054368144	27.80643943844508	24.70581742700178
49	17.236339425271396	27.26453822682233	31.788630043829198	23.71049230407708
50	16.751227466772058	28.99037965128906	31.288119118573825	22.970273763365057
51	16.478229599484447	26.58698368955683	28.494408305828824	28.440378405129902
52	17.876865233227587	24.661044708630186	30.670024175487693	26.79206588265453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1943.0
1	1886.5
2	1830.0
3	13577.0
4	25324.0
5	14314.0
6	3304.0
7	4373.0
8	5442.0
9	7542.5
10	9643.0
11	11933.5
12	14224.0
13	14497.0
14	13964.5
15	13159.0
16	11571.5
17	9984.0
18	8910.0
19	7836.0
20	7100.0
21	6364.0
22	5997.0
23	5630.0
24	5607.0
25	5584.0
26	6070.0
27	6556.0
28	7029.5
29	7503.0
30	8224.0
31	8945.0
32	10877.5
33	12810.0
34	15972.5
35	19135.0
36	21069.0
37	23003.0
38	25725.0
39	29128.5
40	29810.0
41	37536.5
42	45263.0
43	58592.5
44	71922.0
45	85950.0
46	99978.0
47	114281.0
48	128584.0
49	140556.0
50	152528.0
51	133972.5
52	115417.0
53	92990.5
54	70564.0
55	59911.5
56	49259.0
57	45661.0
58	42063.0
59	37604.5
60	33146.0
61	27071.0
62	20996.0
63	17286.0
64	10744.5
65	7913.0
66	5735.0
67	3557.0
68	3004.5
69	2452.0
70	2163.0
71	1874.0
72	1766.0
73	1658.0
74	1237.5
75	817.0
76	573.0
77	329.0
78	250.0
79	171.0
80	125.0
81	79.0
82	57.5
83	36.0
84	20.0
85	4.0
86	9.0
87	14.0
88	8.0
89	1.0
90	0.0
91	2.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1123452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.51628691903826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22615745779204	18.886532272914106
2	11.970772616024286	6.348408826581324
3	4.878037392643064	3.880423173153623
4	2.574242579837985	2.730374193847571
5	1.526493134016676	2.023846496076405
6	1.006978217065457	1.602079399495755
7	0.7147624275475887	1.326699192546011
8	0.5602393612704014	1.1884374117427756
9	0.43467238114072904	1.0373307816698214
>10	3.9082030503781393	22.162925442618267
>50	0.8265965418377341	15.825312521384975
>100	0.356326398147667	13.683004722454806
>500	0.009101998817390157	1.6731002364252074
>1k	0.006067999211593438	3.1067264367664738
>5k	6.742221346214931E-4	1.4840417868852538
>10k+	6.742221346214931E-4	3.0407571054376388
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23322	2.0759231369030453	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10695	0.9519765864496214	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	8709	0.7752000085450914	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7893	0.7025667318229885	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3993	0.3554223945482317	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3028	0.2695264239148624	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	2871	0.2555516390553402	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2625	0.23365484239647089	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2361	0.21015584110402583	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2001	0.17811174843250982	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1968	0.17517437327095417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	1881	0.16743038420867112	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1783	0.1587072700925362	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1768	0.1573720995645564	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	1718	0.15292153113795695	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	1648	0.14669073534071772	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1361	0.12114447257203691	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1223	0.10886090371462244	No Hit
GGACAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1209	0.10761474455517459	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1183	0.10530044897334287	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2631176053805592	0.0
2	0.0	0.0	0.0	0.8289628751384127	0.0
3	0.0	0.0	0.0	1.6663818302873643	0.0
4	0.0	0.0	0.0	2.2908855919077986	0.0
5	0.0	0.0	0.0	5.288788484065185	0.0
6	0.0	0.0	0.0	6.339300655479718	0.0
7	0.0	0.0	0.0	8.623332372010553	0.0
8	0.0	0.0	0.0	11.534360168480719	0.0
9	0.0	0.0	0.0	13.876160263188815	0.0
10	0.0	0.0	0.0	15.527944229036933	0.0
11	0.0	0.0	0.0	16.93307769268291	0.0
12	0.0	0.0	0.0	17.901343359573886	0.0
13	0.0	0.0	0.0	18.359128827933905	0.0
14	0.0	0.0	0.0	18.621000274155016	0.0
15	0.0	0.0	0.0	19.25217988841535	0.0
16	0.0	0.0	0.0	20.157781551859802	0.0
17	0.0	0.0	0.0	21.170107846174112	0.0
18	0.0	0.0	0.0	21.69545294324991	0.0
19	0.0	0.0	0.0	22.248836621413286	0.0
20	0.0	0.0	0.0	22.862391984704285	0.0
21	0.0	0.0	0.0	23.51680356615147	0.0
22	0.0	0.0	0.0	24.14789416904327	0.0
23	0.0	0.0	0.0	24.592862000334684	0.0
24	0.0	0.0	0.0	24.92612056411845	0.0
25	0.0	0.0	0.0	25.29080014099401	0.0
26	0.0	0.0	0.0	25.765052712532444	0.0
27	0.0	0.0	0.0	26.162399461659245	0.0
28	0.0	0.0	0.0	26.64510811320822	0.0
29	8.901136853198891E-5	0.0	0.0	27.048596646763723	0.0
30	8.901136853198891E-5	0.0	0.0	27.461520385383622	0.0
31	8.901136853198891E-5	0.0	0.0	27.762378811021744	0.0
32	8.901136853198891E-5	0.0	0.0	28.154918946247815	0.0
33	8.901136853198891E-5	0.0	0.0	28.59205377710841	0.0
34	8.901136853198891E-5	0.0	0.0	29.151579239700496	0.0
35	8.901136853198891E-5	0.0	0.0	29.51919619173761	0.0
36	8.901136853198891E-5	0.0	0.0	29.925355066349073	0.0
37	8.901136853198891E-5	0.0	0.0	30.498321245589487	0.0
38	8.901136853198891E-5	0.0	0.0	31.89028102669273	0.0
39	1.7802273706397781E-4	0.0	0.0	32.383493019728476	0.0
40	1.7802273706397781E-4	0.0	0.0	32.809857474996704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGG	55	1.8189894E-12	46.000004	1
CCTACGG	55	1.8189894E-12	46.000004	1
ACTACGG	70	0.0	46.000004	1
GCGATCG	35	1.01954356E-7	46.000004	8
TGGGCGC	140	0.0	46.000004	5
GCTACGG	220	0.0	46.000004	1
GTTATCT	215	0.0	46.000004	24
ATTTAGG	160	0.0	46.0	1
TCGCCCA	30	1.8613646E-6	46.0	45
CGGGTAC	100	0.0	46.0	5
GCGACTA	20	6.311393E-4	46.0	28
CCCTCGC	20	6.311393E-4	46.0	42
CGACGCG	20	6.311393E-4	46.0	46
GCAACCG	20	6.311393E-4	46.0	8
GGCGTGC	20	6.311393E-4	46.0	19
CGGCACT	30	1.8613646E-6	46.0	39
TCCCTCG	20	6.311393E-4	46.0	41
ATTCGGC	80	0.0	46.0	16
CCGTCGG	25	3.4171055E-5	46.0	34
GTGGACG	20	6.311393E-4	46.0	12
>>END_MODULE