FastQCFastQC Report
Sat 14 Jan 2017
SRR2933042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306375
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62602.0432476540187676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17160.5600979192166462No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10900.3557731538147695No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG9800.3198694410444717No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT9580.3126886984904121TruSeq Adapter, Index 8 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT7550.24643002855977153TruSeq Adapter, Index 13 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6790.22162382700938393No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6070.19812321501427987No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT5970.19485924112607098TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC4950.16156670746634028No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC3600.1175030599755202TruSeq Adapter, Index 8 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT3390.11064871481028152No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC3360.10966952264381885No Hit
AAAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT3290.10738474092207263TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC3120.1018359853121175TruSeq Adapter, Index 13 (95% over 23bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC3080.10053039575683394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1450.046.0000045
TCACGAC501.6370905E-1146.024
GGTACAT206.3041726E-446.07
CATATCC253.4112494E-546.043
GATCGAA405.5879354E-946.08
CGACGGT501.6370905E-1146.027
CATGCGG253.4112494E-546.01
TTGTCGC301.8569026E-646.037
GCAAATC253.4112494E-546.09
CACGCGT206.3041726E-446.038
CCTACGG206.3041726E-446.01
CACGACG501.6370905E-1146.025
GTAATTG253.4112494E-546.040
GCACCGG501.6370905E-1146.01
CTAACGG301.8569026E-646.01
CTAAATG253.4112494E-546.010
AACATAC501.6370905E-1146.015
CGAGGTC206.3041726E-446.030
CCGAGGT206.3041726E-446.029
ACTACGG253.4112494E-546.01
Produced by FastQC (version 0.11.5)