##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933042.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306375
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4989898000816	33.0	31.0	34.0	31.0	34.0
2	32.82593227254182	34.0	31.0	34.0	31.0	34.0
3	32.83527376580987	34.0	31.0	34.0	31.0	34.0
4	36.31819175846593	37.0	37.0	37.0	35.0	37.0
5	36.29994288045695	37.0	37.0	37.0	35.0	37.0
6	36.45223011015912	37.0	37.0	37.0	35.0	37.0
7	36.51277356181151	37.0	37.0	37.0	35.0	37.0
8	36.34873929008568	37.0	37.0	37.0	35.0	37.0
9	38.01337576499388	39.0	39.0	39.0	35.0	39.0
10	37.87903386372909	39.0	38.0	39.0	35.0	39.0
11	37.3543533251734	39.0	35.0	39.0	35.0	39.0
12	37.16685434516524	39.0	35.0	39.0	34.0	39.0
13	37.143170950632395	39.0	35.0	39.0	34.0	39.0
14	38.54115381476948	40.0	36.0	41.0	35.0	41.0
15	38.62363117095063	41.0	36.0	41.0	35.0	41.0
16	38.63228070175439	41.0	36.0	41.0	35.0	41.0
17	38.628853529171764	40.0	36.0	41.0	35.0	41.0
18	38.42621950224398	40.0	36.0	41.0	35.0	41.0
19	38.20227498980008	40.0	36.0	41.0	35.0	41.0
20	38.00214443084455	40.0	35.0	41.0	35.0	41.0
21	37.950488780089756	40.0	35.0	41.0	34.0	41.0
22	37.90167278661771	40.0	35.0	41.0	34.0	41.0
23	37.85636230110159	40.0	35.0	41.0	34.0	41.0
24	37.7629898000816	40.0	35.0	41.0	34.0	41.0
25	37.7007327621379	40.0	35.0	41.0	34.0	41.0
26	37.62171848225214	39.0	35.0	41.0	34.0	41.0
27	37.54498245614035	39.0	35.0	41.0	34.0	41.0
28	37.5225458996328	39.0	35.0	41.0	34.0	41.0
29	37.44854834761322	39.0	35.0	41.0	34.0	41.0
30	37.27499306405549	39.0	35.0	41.0	34.0	41.0
31	37.10494655242758	39.0	35.0	41.0	33.0	41.0
32	36.88388086495308	39.0	35.0	41.0	33.0	41.0
33	36.56446185230518	39.0	35.0	41.0	32.0	41.0
34	36.35776091391269	39.0	35.0	41.0	31.0	41.0
35	36.16313341493268	39.0	35.0	41.0	31.0	41.0
36	36.05764830681355	39.0	35.0	41.0	30.0	41.0
37	35.94977396980824	38.0	35.0	41.0	30.0	41.0
38	35.91812321501428	38.0	35.0	41.0	30.0	41.0
39	35.79285516115871	38.0	35.0	41.0	29.0	41.0
40	35.758178702570376	38.0	35.0	41.0	30.0	41.0
41	35.71331864545083	38.0	35.0	41.0	30.0	41.0
42	35.631866177070584	38.0	35.0	41.0	29.0	41.0
43	35.48516687066503	38.0	35.0	41.0	29.0	41.0
44	35.38247246022032	38.0	35.0	41.0	28.0	41.0
45	35.30921256629947	37.0	35.0	41.0	27.0	41.0
46	35.241909424724604	37.0	35.0	41.0	27.0	41.0
47	35.16284618523052	37.0	35.0	40.0	27.0	41.0
48	35.12580334557324	37.0	35.0	40.0	27.0	41.0
49	35.02927458180334	37.0	35.0	40.0	27.0	41.0
50	34.910877192982454	36.0	35.0	40.0	26.0	41.0
51	34.792192574459406	36.0	35.0	40.0	26.0	41.0
52	34.56379926560587	36.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	2.0
15	11.0
16	19.0
17	34.0
18	77.0
19	153.0
20	271.0
21	376.0
22	625.0
23	1153.0
24	1941.0
25	3277.0
26	4395.0
27	4972.0
28	4837.0
29	4557.0
30	4577.0
31	5156.0
32	6312.0
33	8183.0
34	18733.0
35	44780.0
36	20428.0
37	22443.0
38	34483.0
39	113497.0
40	1078.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.935373317013465	21.031742146062832	23.195430436556506	23.837454100367196
2	31.528682170542638	23.6031007751938	24.533659730722153	20.33455732354141
3	25.59869441044472	23.745410036719704	30.828559771521828	19.82733578131375
4	23.146797225622194	27.70983272133823	28.464137086903307	20.679232966136272
5	20.32705018359853	35.27768257853937	26.452223582211342	17.943043655650754
6	79.2936760505916	4.703059975520196	13.560832313341493	2.4424316605467156
7	81.60848633210934	3.7434516523867813	11.39485924112607	3.253202774377805
8	74.5419828641371	5.617625458996328	15.348510811913505	4.49188086495308
9	42.22374541003672	24.038514891880865	19.201305589555282	14.536434108527132
10	35.0671562627499	21.65907792737658	28.048306813545494	15.225458996328028
11	30.19143206854345	21.198204814361485	31.03516931864545	17.57519379844961
12	19.98922888616891	29.973072215422274	32.954385964912284	17.083312933496533
13	15.231660546715625	34.09024887800898	34.80277437780498	15.87531619747042
14	14.475397796817626	25.81019991840065	42.91701346389229	16.797388820889434
15	16.04798041615667	20.84798041615667	42.82268461852305	20.28135454916361
16	16.091064871481027	23.098490412076703	31.78718890248878	29.023255813953487
17	18.758057935536517	24.167768257853936	35.72158302733578	21.35259077927377
18	22.89906160750714	27.61485108119135	30.614443084455324	18.871644226846186
19	24.40636474908201	23.950387596899226	30.04618523051816	21.597062423500613
20	19.401060791513668	29.25891472868217	31.529008567931456	19.811015911872705
21	18.503141574867403	25.21452468380253	31.327294981640147	24.955038759689923
22	16.06854345165239	29.636882904936762	29.525254997960015	24.769318645450834
23	16.57739698082415	26.621950224398205	37.80563035495716	18.99502243982048
24	19.506487148102817	24.96940024479804	35.40693594451245	20.1171766625867
25	15.186617707058344	31.820481436148512	30.16825785393717	22.824643002855975
26	14.622929416564666	30.28184414524684	31.984006527947777	23.111219910240717
27	17.84643002855977	27.161485108119138	36.04439004487964	18.94769481844145
28	15.979110567115463	26.796572827417382	34.00114239086087	23.22317421460628
29	18.61281109751122	27.481680946552427	31.349490004079968	22.556017951856386
30	24.93643410852713	29.01281109751122	26.798531211750305	19.252223582211343
31	25.437127702978373	27.249612403100777	27.297266421868628	20.015993472052223
32	23.716034271725825	27.792084863321094	25.946960424316607	22.544920440636478
33	18.12060383516932	29.69596083231334	28.138718890248875	24.044716442268463
34	19.135046919624642	25.667890656874743	28.789881680946554	26.40718074255406
35	25.635577315381475	26.164667482660136	28.21183190534476	19.987923296613626
36	19.59657282741738	30.646103631170952	26.365075479396165	23.392248062015504
37	21.34606283149735	29.93064055487556	27.27017543859649	21.453121175030603
38	19.271807425540597	27.689269685842515	27.500938392492856	25.537984496124032
39	22.619012647898817	25.15479396164831	28.789881680946554	23.436311709506324
40	16.252957976336187	24.653773969808242	31.80448796409629	27.28878008975928
41	19.896532027743778	27.769889840881273	26.02627498980008	26.307303141574867
42	20.402121583027334	24.849612403100778	29.459322725418197	25.28894328845369
43	21.457690738474092	27.022113423092613	29.653855569155446	21.866340269277845
44	19.15658914728682	30.599428804569563	25.84283965728274	24.401142390860873
45	21.125091799265604	28.120440636474907	27.637698898408814	23.116768665850675
46	22.090575275397796	26.310240718074258	27.89033047735618	23.708853529171765
47	18.82562219502244	25.842186862505102	33.08625050999592	22.24594043247654
48	19.595267237862096	24.593064055487556	30.55667074663403	25.25499796001632
49	19.769889840881273	26.432966136270906	30.91342309261526	22.88372093023256
50	18.08535291717666	26.57951856385149	30.669604243166056	24.665524275805794
51	19.062913096695226	23.733333333333334	30.640881272949816	26.562872297021624
52	20.194206446348428	22.982292941656468	32.61036311709506	24.213137494900042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1853.0
1	1259.0
2	665.0
3	4462.0
4	8259.0
5	5092.5
6	1926.0
7	2125.5
8	2325.0
9	2559.0
10	2793.0
11	2921.5
12	3050.0
13	3189.0
14	3074.5
15	2821.0
16	2582.0
17	2343.0
18	2242.5
19	2142.0
20	2167.5
21	2193.0
22	2037.5
23	1882.0
24	1794.5
25	1707.0
26	1870.5
27	2034.0
28	2237.0
29	2440.0
30	2470.0
31	2500.0
32	2986.0
33	3472.0
34	4040.0
35	4608.0
36	5323.5
37	6039.0
38	6176.5
39	7319.5
40	8325.0
41	11280.0
42	14235.0
43	16861.0
44	19487.0
45	21778.0
46	24069.0
47	23954.0
48	23839.0
49	26685.0
50	29531.0
51	29774.5
52	30018.0
53	26728.0
54	23438.0
55	20426.5
56	17415.0
57	15408.0
58	13401.0
59	12108.0
60	10815.0
61	9770.0
62	8725.0
63	7372.0
64	5052.0
65	4085.0
66	3551.5
67	3018.0
68	2278.0
69	1538.0
70	1418.5
71	1299.0
72	1236.0
73	1173.0
74	884.0
75	595.0
76	441.0
77	287.0
78	246.0
79	205.0
80	158.0
81	111.0
82	67.0
83	23.0
84	20.0
85	17.0
86	13.5
87	10.0
88	6.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	306375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.84504435790254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43590613516108	24.854415006072937
2	9.781764715721463	6.621285214043176
3	3.6103442142126423	3.6657678023197096
4	1.9033691051462207	2.576784471725404
5	1.2450037073263034	2.106860285010592
6	0.9564397284705937	1.9422447021448501
7	0.790707312957336	1.8733066856811271
8	0.6858783883794186	1.8570867583062503
9	0.6239795761577429	1.9006754790135627
>10	6.807866713570236	43.01101862916209
>50	0.10982040451992657	2.4906451276546253
>100	0.03993469255270057	2.4903072297944093
>500	0.005990203882905085	1.5462206083499614
>1k	0.0019967346276350286	0.9481413957670437
>5k	9.983673138175143E-4	2.115240604954274
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6260	2.0432476540187676	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1716	0.5600979192166462	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1090	0.3557731538147695	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	980	0.3198694410444717	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT	958	0.3126886984904121	TruSeq Adapter, Index 8 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	755	0.24643002855977153	TruSeq Adapter, Index 13 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	679	0.22162382700938393	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	607	0.19812321501427987	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	597	0.19485924112607098	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC	495	0.16156670746634028	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	360	0.1175030599755202	TruSeq Adapter, Index 8 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	339	0.11064871481028152	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	336	0.10966952264381885	No Hit
AAAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	329	0.10738474092207263	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	312	0.1018359853121175	TruSeq Adapter, Index 13 (95% over 23bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	308	0.10053039575683394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3215014279885761	0.0
2	0.0	0.0	0.0	0.9361077111383109	0.0
3	0.0	0.0	0.0	1.626111791105671	0.0
4	0.0	0.0	0.0	2.116360669114647	0.0
5	0.0	0.0	0.0	4.4980824153406775	0.0
6	0.0	0.0	0.0	4.960587515299878	0.0
7	0.0	0.0	0.0	6.069359445124439	0.0
8	0.0	0.0	0.0	7.536842105263158	0.0
9	0.0	0.0	0.0	8.67923296613627	0.0
10	0.0	0.0	0.0	9.747205222358222	0.0
11	0.0	0.0	0.0	11.022113423092616	0.0
12	0.0	0.0	0.0	11.97062423500612	0.0
13	0.0	0.0	0.0	12.358384332925336	0.0
14	0.0	0.0	0.0	12.61656466748266	0.0
15	0.0	0.0	0.0	13.42015503875969	0.0
16	3.263973888208894E-4	0.0	0.0	14.369318645450836	0.0
17	3.263973888208894E-4	0.0	0.0	15.53064055487556	0.0
18	3.263973888208894E-4	0.0	0.0	16.067564259485923	0.0
19	3.263973888208894E-4	0.0	0.0	16.61591187270502	0.0
20	3.263973888208894E-4	0.0	0.0	17.268706650346797	0.0
21	0.0013055895552835576	0.0	0.0	17.881354549163607	0.0
22	0.0016319869441044472	0.0	0.0	18.54981640146879	0.0
23	0.0016319869441044472	0.0	0.0	19.05801713586291	0.0
24	0.0019583843329253367	0.0	0.0	19.47906976744186	0.0
25	0.0019583843329253367	0.0	0.0	19.887392900856792	0.0
26	0.0019583843329253367	0.0	0.0	20.2999592003264	0.0
27	0.0019583843329253367	0.0	0.0	20.746144430844552	0.0
28	0.0019583843329253367	0.0	0.0	21.208649530803754	0.0
29	0.0019583843329253367	0.0	0.0	21.69857201142391	0.0
30	0.0019583843329253367	0.0	0.0	22.16172990616075	0.0
31	0.0019583843329253367	0.0	0.0	22.56058751529988	0.0
32	0.0019583843329253367	0.0	0.0	22.971195430436556	0.0
33	0.0019583843329253367	0.0	0.0	23.4062831497348	0.0
34	0.0019583843329253367	0.0	0.0	23.948102815177478	0.0
35	0.0019583843329253367	0.0	0.0	24.362627498980007	0.0
36	0.0019583843329253367	0.0	0.0	24.80359037127703	0.0
37	0.0019583843329253367	0.0	0.0	25.18841289269686	0.0
38	0.0019583843329253367	0.0	0.0	25.65842513259894	0.0
39	0.0019583843329253367	0.0	0.0	26.129416564667483	0.0
40	0.0019583843329253367	0.0	0.0	26.574622603019176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGCGA	145	0.0	46.000004	5
TCACGAC	50	1.6370905E-11	46.0	24
GGTACAT	20	6.3041726E-4	46.0	7
CATATCC	25	3.4112494E-5	46.0	43
GATCGAA	40	5.5879354E-9	46.0	8
CGACGGT	50	1.6370905E-11	46.0	27
CATGCGG	25	3.4112494E-5	46.0	1
TTGTCGC	30	1.8569026E-6	46.0	37
GCAAATC	25	3.4112494E-5	46.0	9
CACGCGT	20	6.3041726E-4	46.0	38
CCTACGG	20	6.3041726E-4	46.0	1
CACGACG	50	1.6370905E-11	46.0	25
GTAATTG	25	3.4112494E-5	46.0	40
GCACCGG	50	1.6370905E-11	46.0	1
CTAACGG	30	1.8569026E-6	46.0	1
CTAAATG	25	3.4112494E-5	46.0	10
AACATAC	50	1.6370905E-11	46.0	15
CGAGGTC	20	6.3041726E-4	46.0	30
CCGAGGT	20	6.3041726E-4	46.0	29
ACTACGG	25	3.4112494E-5	46.0	1
>>END_MODULE