FastQCFastQC Report
Sat 14 Jan 2017
SRR2933041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238082
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC193620.8651157553655318No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190890.8529178108755623No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC139190.6219164445270549No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC136780.6111482957282173No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT63340.2830101846134324Illumina Single End Adapter 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT61320.2739845993131619Illumina Single End Adapter 1 (95% over 23bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC51940.23207371311685632No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC50440.22537154581467525No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT40860.1825670373114122Illumina Single End Adapter 1 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC31760.14190722234484707Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26480.11831559344116971No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC23630.1055814755670257Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG23080.10312401422289265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG1400.046.0000041
TATCCGT351.0201438E-746.00000443
CGTATCG206.312743E-446.038
GGTCGTA206.312743E-446.08
GACCGTA405.6152203E-946.015
TACGCTT206.312743E-446.09
TGCGATT253.418202E-546.042
GTCGTAC206.312743E-446.015
GTATCGC206.312743E-446.039
ACGTCGT253.418202E-546.039
CGTAAGT206.312743E-446.016
CCGCGTA206.312743E-446.013
GTAAGCG253.418202E-546.017
TCACGTA253.418202E-546.014
ACGTAGG2850.045.192981
ATTGCGG4700.045.021281
AATGCGG2300.045.01
CGACGGT6350.044.91338327
CACGACG6600.043.56060825
AGCTACG1650.043.2121249
Produced by FastQC (version 0.11.5)