##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933041.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2238082 Sequences flagged as poor quality 0 Sequence length 52 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51393380582123 33.0 31.0 34.0 31.0 34.0 2 32.84890991482886 34.0 31.0 34.0 31.0 34.0 3 32.92542096312825 34.0 31.0 34.0 31.0 34.0 4 36.35012792203324 37.0 37.0 37.0 35.0 37.0 5 36.32729140397894 37.0 37.0 37.0 35.0 37.0 6 36.47365914206897 37.0 37.0 37.0 35.0 37.0 7 36.54155924581852 37.0 37.0 37.0 35.0 37.0 8 36.37945571252528 37.0 37.0 37.0 35.0 37.0 9 38.03272400206963 39.0 39.0 39.0 35.0 39.0 10 37.92716352662682 39.0 38.0 39.0 35.0 39.0 11 37.46134770754602 39.0 37.0 39.0 35.0 39.0 12 37.293081754823994 39.0 37.0 39.0 35.0 39.0 13 37.07056131097967 39.0 37.0 39.0 33.0 39.0 14 38.579506470272314 41.0 37.0 41.0 34.0 41.0 15 38.69985416083951 41.0 37.0 41.0 35.0 41.0 16 38.716166342430704 41.0 37.0 41.0 35.0 41.0 17 38.73426621544698 41.0 37.0 41.0 35.0 41.0 18 38.66854118839256 40.0 37.0 41.0 35.0 41.0 19 38.55406951130477 40.0 37.0 41.0 35.0 41.0 20 38.47409835743284 40.0 36.0 41.0 35.0 41.0 21 38.44884235698245 40.0 35.0 41.0 35.0 41.0 22 38.420397465329685 40.0 35.0 41.0 35.0 41.0 23 38.38464944537331 40.0 35.0 41.0 35.0 41.0 24 38.32355874360278 40.0 35.0 41.0 35.0 41.0 25 38.258478018231685 40.0 35.0 41.0 35.0 41.0 26 38.16436975946368 40.0 35.0 41.0 34.0 41.0 27 38.0896709772028 40.0 35.0 41.0 34.0 41.0 28 38.07777731110835 40.0 35.0 41.0 34.0 41.0 29 38.04112181769926 40.0 35.0 41.0 34.0 41.0 30 37.879393158963794 40.0 35.0 41.0 34.0 41.0 31 37.86762683404808 40.0 35.0 41.0 34.0 41.0 32 37.764733374380384 40.0 35.0 41.0 34.0 41.0 33 37.62341996405851 40.0 35.0 41.0 34.0 41.0 34 37.50131585884699 40.0 35.0 41.0 33.0 41.0 35 37.424230658215386 40.0 35.0 41.0 33.0 41.0 36 37.33328850328093 40.0 35.0 41.0 33.0 41.0 37 37.23567322376928 40.0 35.0 41.0 33.0 41.0 38 37.24497985328509 40.0 35.0 41.0 33.0 41.0 39 37.16974936575157 39.0 35.0 41.0 33.0 41.0 40 37.11585857890819 39.0 35.0 41.0 33.0 41.0 41 37.1055586881982 39.0 35.0 41.0 33.0 41.0 42 37.05747287186082 39.0 35.0 41.0 33.0 41.0 43 36.971343766671644 39.0 35.0 41.0 33.0 41.0 44 36.882733072336045 39.0 35.0 41.0 33.0 41.0 45 36.836951014306 39.0 35.0 41.0 33.0 41.0 46 36.74333112012875 39.0 35.0 41.0 33.0 41.0 47 36.6198222406507 38.0 35.0 41.0 32.0 41.0 48 36.575269360103874 38.0 35.0 41.0 32.0 41.0 49 36.49504575793023 38.0 35.0 41.0 32.0 41.0 50 36.38359005612842 38.0 35.0 41.0 32.0 41.0 51 36.281825241434404 38.0 35.0 41.0 32.0 41.0 52 36.04075543255341 37.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 4.0 13 9.0 14 10.0 15 38.0 16 75.0 17 165.0 18 334.0 19 612.0 20 1061.0 21 1749.0 22 2745.0 23 4187.0 24 6950.0 25 10389.0 26 14642.0 27 17542.0 28 19430.0 29 22114.0 30 26315.0 31 31919.0 32 41147.0 33 56674.0 34 146454.0 35 263697.0 36 141282.0 37 171381.0 38 280084.0 39 963850.0 40 13221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.521263295982905 22.116213793775206 24.066991289863374 18.295531620378522 2 28.202183834193743 24.389097450406197 26.365075095550566 21.043643619849497 3 28.77486168960744 24.173734474429445 26.145735500307854 20.90566833565526 4 24.153493929176857 27.636833681697098 24.075972194048298 24.13370019507775 5 22.058485792745753 38.782180456301425 21.33603683868598 17.82329691226684 6 87.08974916915466 2.757852482616812 7.821518603875997 2.3308797443525306 7 88.87534058180174 2.021418339453157 5.721014690257104 3.382226388488 8 80.90489982047127 4.396532388000082 9.851292311899208 4.847275479629433 9 40.82370529766112 29.24446021191359 14.92679892872558 15.005035561699707 10 35.205993346088306 21.74795204107803 24.796365816802066 18.249688796031602 11 31.556171757781886 20.226470701252232 28.9501903862325 19.267167154733382 12 24.05112949391488 26.510109995969767 31.726004677219155 17.712755832896203 13 18.37953211723252 32.029925623815394 32.15306677771414 17.437475481237954 14 16.88445731657732 28.081366098293092 36.45670712690598 18.577469458223604 15 20.987524138972567 21.266468342089343 38.56002595079179 19.185981568146296 16 20.748837620784226 24.441642442055294 26.93717209646474 27.87234784069574 17 23.00434032354489 27.86560099227821 28.657529080703924 20.472529603472974 18 25.293800673969947 31.249882712072214 25.398845976152796 18.057470637805046 19 23.053042739274073 27.391623720667962 24.355318527203202 25.20001501285476 20 22.988791295403832 27.935035445528804 29.175115120893697 19.90105813817367 21 19.307290796315772 25.032997003684404 29.66969038667931 25.990021813320514 22 17.073994607883 31.004985518850514 27.011700196864997 24.90931967640149 23 17.602304115756258 31.909018525684047 30.20188715158783 20.286790206971865 24 25.299073045580993 25.08402283741168 27.552743822612396 22.064160294394934 25 17.632463868616075 29.258087952094698 25.445984552844802 27.66346362644443 26 17.323940767139007 27.29301249909521 32.24939926240415 23.13364747136164 27 20.847895653510463 26.01830495933572 32.58231825286116 20.55148113429267 28 18.84783488719359 30.67917082573382 27.668512592478738 22.804481694593854 29 24.897881310872435 25.08093984045267 29.51317243961571 20.508006409059185 30 28.24655218173418 25.019101176811215 27.37906832725521 19.35527831419939 31 29.434176227680663 27.800143158293572 23.78858326013077 18.977097353894987 32 25.94489388681916 25.50214871483708 25.40376089884106 23.1491964995027 33 23.68210816225679 25.481193271738928 27.547784218808786 23.2889143471955 34 21.31231116643626 28.24422876373609 27.24042282633076 23.203037243496887 35 31.839896840240883 25.231783285867092 25.593432233492784 17.334887640399234 36 23.556107416975784 30.743824399642193 24.752846410453238 20.947221772928785 37 24.800521160529417 30.980634310985927 24.85570233798404 19.363142190500614 38 22.433807161667893 27.02479176366192 24.28646492845213 26.254936146218057 39 25.826667655608688 26.109365072414686 27.853537091134285 20.210430180842344 40 20.28790723485556 24.73725270119683 30.60357037856522 24.371269685382394 41 22.27688708456616 29.714550226488573 25.342949900852606 22.665612788092663 42 21.82842272981955 23.80565144619366 28.76677440772947 25.599151416257314 43 23.774776795488282 26.266910685131283 29.88147887342823 20.076833645952206 44 22.773741087234516 27.532056466206335 25.41533330771616 24.278869138842992 45 25.203902269890023 25.365469182987933 26.881320702279897 22.549307844842144 46 26.17151650386357 25.245545069394243 27.716857559285135 20.866080867457047 47 21.58388298552064 29.21559621139887 28.302761024841804 20.89775977823869 48 22.222554848303144 26.534818652757135 27.37656618479573 23.866060314143986 49 21.081667249010536 25.42310782178669 30.676221872120863 22.819003057081915 50 20.585081333034267 26.717653776760635 30.129548425839626 22.56771646436547 51 20.892934217781118 24.34428229171228 27.655421025681807 27.107362464824792 52 21.22991918973478 22.98293806929326 30.078433229881657 25.708709511090298 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3084.0 1 3280.0 2 3476.0 3 13452.0 4 23428.0 5 13999.0 6 4570.0 7 5648.5 8 6727.0 9 8810.0 10 10893.0 11 12585.5 12 14278.0 13 14494.5 14 13517.0 15 12323.0 16 11157.0 17 9991.0 18 8723.0 19 7455.0 20 7438.5 21 7422.0 22 7414.5 23 7407.0 24 7542.5 25 7678.0 26 8498.0 27 9318.0 28 11474.5 29 13631.0 30 15122.5 31 16614.0 32 20881.5 33 25149.0 34 28927.0 35 32705.0 36 37441.5 37 42178.0 38 46350.5 39 53896.5 40 57270.0 41 75726.5 42 94183.0 43 117108.0 44 140033.0 45 158220.0 46 176407.0 47 202264.5 48 228122.0 49 255978.0 50 283834.0 51 257955.0 52 232076.0 53 196247.5 54 160419.0 55 145120.5 56 129822.0 57 122149.0 58 114476.0 59 100063.5 60 85651.0 61 79063.0 62 72475.0 63 61963.5 64 40707.5 65 29963.0 66 24227.0 67 18491.0 68 16560.5 69 14630.0 70 12065.5 71 9501.0 72 8339.0 73 7177.0 74 5904.5 75 4632.0 76 3245.5 77 1859.0 78 1502.5 79 1146.0 80 819.5 81 493.0 82 354.0 83 215.0 84 148.0 85 81.0 86 67.0 87 53.0 88 41.0 89 21.5 90 14.0 91 11.0 92 8.0 93 7.0 94 6.0 95 3.5 96 1.0 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 2238082.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.45497975690504 #Duplication Level Percentage of deduplicated Percentage of total 1 70.24407569549422 13.665970706983455 2 11.99815237729539 4.668476232410878 3 4.944828516447823 2.8860461606657757 4 2.7028481207366912 2.1033542189968464 5 1.5290063885227965 1.4873394168444747 6 1.046129931790693 1.221146198764823 7 0.6919368283664178 0.9423131892248015 8 0.5175935214864289 0.8055817186258936 9 0.42113989787880945 0.7373941369251567 >10 3.6617523988489546 15.8603376540938 >50 1.1423486898399169 16.134028794496498 >100 1.0814973664880099 32.137660302094915 >500 0.01176794576538111 1.6584606858204176 >1k 0.005076368761536949 1.7077063379518869 >5k 9.229761384612634E-4 1.0192099234210363 >10k+ 9.229761384612634E-4 2.964974322679378 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 19362 0.8651157553655318 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19089 0.8529178108755623 No Hit GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 13919 0.6219164445270549 No Hit GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 13678 0.6111482957282173 No Hit CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT 6334 0.2830101846134324 Illumina Single End Adapter 1 (95% over 24bp) CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT 6132 0.2739845993131619 Illumina Single End Adapter 1 (95% over 23bp) AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 5194 0.23207371311685632 No Hit TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 5044 0.22537154581467525 No Hit GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT 4086 0.1825670373114122 Illumina Single End Adapter 1 (95% over 23bp) ACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC 3176 0.14190722234484707 Illumina Single End Adapter 1 (95% over 22bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2648 0.11831559344116971 No Hit CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC 2363 0.1055814755670257 Illumina Single End Adapter 1 (95% over 22bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG 2308 0.10312401422289265 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.2908740609146582 0.0 2 0.0 0.0 0.0 0.9005031987210478 0.0 3 0.0 0.0 0.0 1.6159372176712024 0.0 4 0.0 0.0 0.0 2.0852229721699205 0.0 5 0.0 0.0 0.0 4.38795361385329 0.0 6 0.0 0.0 0.0 4.957995283461464 0.0 7 0.0 0.0 0.0 6.518036425832476 0.0 8 0.0 0.0 0.0 8.520107842340003 0.0 9 0.0 0.0 0.0 9.990831435130616 0.0 10 0.0 0.0 0.0 11.064027144671195 0.0 11 0.0 0.0 0.0 12.0820863578725 0.0 12 0.0 0.0 0.0 12.816911980883631 0.0 13 0.0 0.0 0.0 13.12208399870961 0.0 14 0.0 0.0 0.0 13.31287236124503 0.0 15 0.0 0.0 0.0 13.859322401949527 0.0 16 0.0 0.0 0.0 14.573237262977853 0.0 17 4.468111534787376E-5 0.0 0.0 15.435493426961122 0.0 18 8.936223069574751E-5 0.0 0.0 15.884047143938426 0.0 19 8.936223069574751E-5 0.0 0.0 16.37460110934273 0.0 20 8.936223069574751E-5 0.0 0.0 16.949781107215912 0.0 21 8.936223069574751E-5 0.0 0.0 17.51338869621399 0.0 22 1.3404334604362128E-4 0.0 0.0 18.074806910560024 0.0 23 1.3404334604362128E-4 0.0 0.0 18.489447660988294 0.0 24 1.3404334604362128E-4 0.0 0.0 18.810124025839983 0.0 25 1.7872446139149503E-4 0.0 0.0 19.13004081173076 0.0 26 1.7872446139149503E-4 0.0 0.0 19.530115518555622 0.0 27 1.7872446139149503E-4 0.0 0.0 19.929564689765613 0.0 28 1.7872446139149503E-4 0.0 0.0 20.36038000394981 0.0 29 2.234055767393688E-4 0.0 0.0 20.746335478324745 0.0 30 2.234055767393688E-4 0.0 0.0 21.134525008467072 0.0 31 2.234055767393688E-4 0.0 0.0 21.460384382699115 0.0 32 2.234055767393688E-4 0.0 0.0 21.862022928561153 0.0 33 2.234055767393688E-4 0.0 0.0 22.27648495452803 0.0 34 2.234055767393688E-4 0.0 0.0 22.790809273297405 0.0 35 2.6808669208724256E-4 0.0 0.0 23.12882191090407 0.0 36 3.5744892278299006E-4 0.0 0.0 23.51656462989292 0.0 37 4.0213003813086386E-4 0.0 0.0 24.0380826082333 0.0 38 4.0213003813086386E-4 0.0 0.0 25.16301904934672 0.0 39 4.0213003813086386E-4 0.0 0.0 25.643430401567056 0.0 40 4.0213003813086386E-4 0.0 0.0 26.040064662510133 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTACGG 140 0.0 46.000004 1 TATCCGT 35 1.0201438E-7 46.000004 43 CGTATCG 20 6.312743E-4 46.0 38 GGTCGTA 20 6.312743E-4 46.0 8 GACCGTA 40 5.6152203E-9 46.0 15 TACGCTT 20 6.312743E-4 46.0 9 TGCGATT 25 3.418202E-5 46.0 42 GTCGTAC 20 6.312743E-4 46.0 15 GTATCGC 20 6.312743E-4 46.0 39 ACGTCGT 25 3.418202E-5 46.0 39 CGTAAGT 20 6.312743E-4 46.0 16 CCGCGTA 20 6.312743E-4 46.0 13 GTAAGCG 25 3.418202E-5 46.0 17 TCACGTA 25 3.418202E-5 46.0 14 ACGTAGG 285 0.0 45.19298 1 ATTGCGG 470 0.0 45.02128 1 AATGCGG 230 0.0 45.0 1 CGACGGT 635 0.0 44.913383 27 CACGACG 660 0.0 43.560608 25 AGCTACG 165 0.0 43.212124 9 >>END_MODULE