##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933040.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3127703
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54483881621753	33.0	31.0	34.0	31.0	34.0
2	32.92016601320522	34.0	31.0	34.0	31.0	34.0
3	32.99408223862688	34.0	33.0	34.0	31.0	34.0
4	36.424042180475574	37.0	37.0	37.0	35.0	37.0
5	36.407967444479226	37.0	37.0	37.0	35.0	37.0
6	36.526459513579134	37.0	37.0	37.0	35.0	37.0
7	36.56349212185428	37.0	37.0	37.0	35.0	37.0
8	36.425467187901155	37.0	37.0	37.0	35.0	37.0
9	38.089235135177475	39.0	39.0	39.0	35.0	39.0
10	38.01181857740329	39.0	39.0	39.0	35.0	39.0
11	37.75083599689612	39.0	37.0	39.0	35.0	39.0
12	37.67524985588465	39.0	38.0	39.0	35.0	39.0
13	37.59459449954168	39.0	38.0	39.0	35.0	39.0
14	39.08000567828851	41.0	39.0	41.0	35.0	41.0
15	39.13582012102812	41.0	39.0	41.0	35.0	41.0
16	39.12713898985933	41.0	39.0	41.0	35.0	41.0
17	39.098226078371255	41.0	38.0	41.0	35.0	41.0
18	39.00520349918135	41.0	38.0	41.0	35.0	41.0
19	38.87057274939468	41.0	38.0	41.0	35.0	41.0
20	38.73215487531905	41.0	37.0	41.0	35.0	41.0
21	38.69901074366716	40.0	37.0	41.0	35.0	41.0
22	38.66229114465152	40.0	37.0	41.0	35.0	41.0
23	38.61670305652423	40.0	37.0	41.0	35.0	41.0
24	38.56421917298414	40.0	37.0	41.0	35.0	41.0
25	38.5152081255797	40.0	36.0	41.0	35.0	41.0
26	38.41241383852623	40.0	36.0	41.0	35.0	41.0
27	38.333935159444486	40.0	36.0	41.0	35.0	41.0
28	38.32412412559633	40.0	36.0	41.0	35.0	41.0
29	38.284939778489196	40.0	36.0	41.0	35.0	41.0
30	38.20677091143245	40.0	36.0	41.0	34.0	41.0
31	38.09956380129443	40.0	35.0	41.0	34.0	41.0
32	37.94483939171974	40.0	35.0	41.0	34.0	41.0
33	37.71577736121365	40.0	35.0	41.0	33.0	41.0
34	37.53180784748424	40.0	35.0	41.0	33.0	41.0
35	37.39859922761208	40.0	35.0	41.0	33.0	41.0
36	37.312582108979015	40.0	35.0	41.0	33.0	41.0
37	37.19966249992407	40.0	35.0	41.0	33.0	41.0
38	37.16048614590324	40.0	35.0	41.0	33.0	41.0
39	37.09674799685264	40.0	35.0	41.0	33.0	41.0
40	37.05360131700484	40.0	35.0	41.0	33.0	41.0
41	37.00564247948095	40.0	35.0	41.0	33.0	41.0
42	36.96095441287104	40.0	35.0	41.0	33.0	41.0
43	36.8809349864741	40.0	35.0	41.0	33.0	41.0
44	36.77509661243411	39.0	35.0	41.0	32.0	41.0
45	36.70014224496379	39.0	35.0	41.0	32.0	41.0
46	36.62115648448718	39.0	35.0	41.0	32.0	41.0
47	36.51900803880675	39.0	35.0	41.0	32.0	41.0
48	36.432503981356284	39.0	35.0	41.0	31.0	41.0
49	36.344260948050376	39.0	35.0	41.0	31.0	41.0
50	36.2486741867754	38.0	35.0	41.0	31.0	41.0
51	36.14262159802257	38.0	35.0	41.0	31.0	41.0
52	35.887856999209966	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	8.0
13	9.0
14	27.0
15	38.0
16	102.0
17	219.0
18	458.0
19	843.0
20	1535.0
21	2584.0
22	4192.0
23	6515.0
24	11525.0
25	20214.0
26	29652.0
27	33259.0
28	33188.0
29	33104.0
30	35481.0
31	40931.0
32	51241.0
33	68523.0
34	157350.0
35	264673.0
36	191394.0
37	254671.0
38	416344.0
39	1454774.0
40	14841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.057229858461625	20.991539158289648	23.891718619063255	21.059512364185473
2	30.51165663747485	23.08844541825103	25.484836635703584	20.91506130857054
3	27.907349259184777	24.065008730048856	27.52489606589884	20.502745944867527
4	23.659343614147506	26.925830233880905	25.694830998979125	23.719995152992468
5	22.21793437548258	37.46893487009476	23.102033664961155	17.2110970894615
6	86.73732768104901	2.6368552257039752	8.458251950392988	2.1675651428540372
7	87.91026513706704	2.1754303397733095	7.415377994649748	2.4989265285099
8	81.3393726961927	4.51647742768415	10.337170760778756	3.8069791153443915
9	38.36710199146147	30.612849110033785	16.569380148946365	14.450668749558382
10	29.361003906061413	23.18909436094156	27.621900161236535	19.82800157176049
11	27.310937131818463	20.65550981023454	32.08645450031541	19.94709855763159
12	23.262343003795436	22.953937761993387	33.35217570210471	20.43154353210647
13	19.148141623421406	28.0613280736694	32.85027382715047	19.940256475758726
14	17.9903590590283	26.600927261955498	35.74859889190246	19.660114787113738
15	21.91448484718658	22.817351903297723	35.5043621469174	19.763801102598297
16	21.897123863742816	23.421181614750505	30.39585280315938	24.2858417183473
17	21.860387639107678	25.997225439883522	31.404164653741102	20.7382222672677
18	24.37123985237729	28.41628505008308	27.94072838757388	19.271746709965747
19	24.323281334576844	25.212624088668267	27.9314564074658	22.53263816928909
20	24.66871055212084	25.56032334272148	29.5330470955842	20.23791900957348
21	21.427705891512076	23.60252875672658	30.02522937759755	24.944535974163788
22	20.03236880228078	28.10263634366818	28.951246329974424	22.913748524076617
23	19.603555708454415	28.19676292793785	30.614639561365003	21.58504180224273
24	24.360081503902382	25.259879214874303	28.69700224094168	21.68303704028164
25	20.069680529129524	26.8714772470404	27.946387492674337	25.112454731155744
26	18.813391169174313	26.179307945799202	31.437032224607	23.570268660419483
27	20.46965456758522	25.31071524374277	32.18882994964675	22.030800239025254
28	20.92708291036585	27.826746976934828	28.52045095074564	22.725719161953677
29	21.955505366078558	26.00956676513083	30.4087056859299	21.62622218286071
30	25.264195481476342	25.512045101469035	26.904632569013103	22.319126848041517
31	25.573272142527596	27.426932800205133	25.681210779923795	21.31858427734347
32	23.822306657633412	27.265536401634044	24.980217111407317	23.931939829325227
33	21.661967264794644	26.330281359834995	27.64798959491998	24.35976178045038
34	21.42460457402765	27.09755369995169	27.872595319952055	23.605246406068606
35	26.20485384961424	27.40304945834051	25.632357036457748	20.759739655587502
36	22.783301355659408	28.264192603965277	26.7635386096442	22.188967430731115
37	22.77012874943689	28.812518324150343	27.203925692433074	21.213427233979697
38	20.945722787617623	26.03674325855108	27.299523004581953	25.718010949249337
39	22.556649400534514	24.70688553228999	30.82632845893616	21.910136608239338
40	19.703789010657342	24.572473793067946	31.948238052014528	23.77549914426018
41	20.847439798471914	28.376863148451115	27.228544398237304	23.54715265483967
42	20.83944671217184	25.08857138929112	28.730317424640383	25.341664473896657
43	22.676034137512417	25.60054455298345	30.256709156847695	21.466712152656438
44	22.836215586965896	26.96016853262602	26.857217581081066	23.346398299327014
45	23.10446356319638	26.496569527221737	27.501907949699827	22.89705895988206
46	23.491616691226756	26.18755681086088	28.038052206363584	22.282774291548783
47	21.27123323410183	27.597888929991115	28.86514480435003	22.265733031557026
48	21.816873277290075	26.609016265291174	28.188705896947376	23.385404560471372
49	20.39455152870973	25.741734429387957	31.051893354324246	22.81182068757807
50	20.177043664312116	25.49318141780086	31.32701538477279	23.002759533114236
51	20.930887619444686	24.43112405493744	29.316722207959007	25.32126611765887
52	20.595561662983986	24.52064662149827	29.266493653649338	25.617298061868404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2759.0
1	3861.0
2	4963.0
3	29957.0
4	54951.0
5	33913.0
6	12875.0
7	13308.5
8	13742.0
9	15346.0
10	16950.0
11	18188.0
12	19426.0
13	19555.5
14	18747.5
15	17810.0
16	16175.5
17	14541.0
18	13804.5
19	13068.0
20	12138.0
21	11208.0
22	11528.5
23	11849.0
24	14405.5
25	16962.0
26	18705.0
27	20448.0
28	24623.0
29	28798.0
30	31663.0
31	34528.0
32	41253.5
33	47979.0
34	55035.0
35	62091.0
36	74416.0
37	86741.0
38	93347.5
39	101440.0
40	102926.0
41	114693.0
42	126460.0
43	145673.0
44	164886.0
45	180254.0
46	195622.0
47	217743.5
48	239865.0
49	269537.5
50	299210.0
51	281542.0
52	263874.0
53	236305.5
54	208737.0
55	194096.0
56	179455.0
57	169763.5
58	160072.0
59	153157.5
60	146243.0
61	137390.0
62	128537.0
63	115874.5
64	86950.0
65	70688.0
66	57453.5
67	44219.0
68	36929.0
69	29639.0
70	25203.0
71	20767.0
72	18041.5
73	15316.0
74	11776.0
75	8236.0
76	6241.5
77	4247.0
78	3123.0
79	1999.0
80	1514.0
81	1029.0
82	882.5
83	736.0
84	506.0
85	276.0
86	182.5
87	89.0
88	53.0
89	11.5
90	6.0
91	6.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3127703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.519058536383866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8764446157377	15.158750915302424
2	9.818784891405304	4.02944443885814
3	3.7378216283478234	2.3008974237189195
4	1.8621790899625765	1.528406470086886
5	1.1755322757827393	1.2060407789097287
6	0.8130603705906412	1.0009940002657995
7	0.6175967360586165	0.8870752505346459
8	0.49041206521715736	0.8050235098511804
9	0.40063737412926875	0.739863155845943
>10	4.832478538749731	23.638639535598085
>50	1.6757240911166618	24.62671373930724
>100	0.6897149948016487	18.830903064263637
>500	0.005358248449231814	0.7257743581295868
>1k	0.0033095063951137675	1.357318343794835
>5k	3.1519108524893024E-4	0.4029205356573985
>10k+	6.303821704978605E-4	2.76123447987557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39196	1.2531880424707844	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	22408	0.7164363112482227	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	12145	0.3883041324575895	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	11640	0.37215809813144024	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6635	0.21213651040396098	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5825	0.18623891079172158	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	3801	0.12152688410632338	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3333	0.10656382655258508	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	3149	0.10068091503573069	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08280837406876548	0.0
2	0.0	0.0	0.0	0.23781030359979832	0.0
3	0.0	0.0	0.0	0.4953795165333793	0.0
4	0.0	0.0	0.0	0.7039670966201075	0.0
5	0.0	0.0	0.0	1.8075245635535087	0.0
6	0.0	0.0	0.0	2.3262438920831037	0.0
7	0.0	0.0	0.0	3.3395754008612712	0.0
8	3.197234520029555E-5	0.0	0.0	4.4654175923992785	0.0
9	3.197234520029555E-5	0.0	0.0	5.557401070370172	0.0
10	3.197234520029555E-5	0.0	0.0	6.267826580720739	0.0
11	3.197234520029555E-5	0.0	0.0	6.838149274403611	0.0
12	3.197234520029555E-5	0.0	0.0	7.215742671219102	0.0
13	3.197234520029555E-5	0.0	0.0	7.422028242451409	0.0
14	3.197234520029555E-5	0.0	0.0	7.5455693843053515	0.0
15	3.197234520029555E-5	0.0	0.0	7.8344714955352215	0.0
16	3.197234520029555E-5	0.0	0.0	8.209858800531892	0.0
17	3.197234520029555E-5	0.0	0.0	8.686438578087497	0.0
18	3.197234520029555E-5	0.0	0.0	8.931314769976561	0.0
19	3.197234520029555E-5	0.0	0.0	9.218042761732812	0.0
20	3.197234520029555E-5	0.0	0.0	9.548732728139468	0.0
21	3.197234520029555E-5	0.0	0.0	9.90707877314438	0.0
22	3.197234520029555E-5	0.0	0.0	10.256216782731608	0.0
23	3.197234520029555E-5	0.0	0.0	10.5364543884122	0.0
24	3.197234520029555E-5	0.0	0.0	10.761060113444275	0.0
25	3.197234520029555E-5	0.0	0.0	10.981637322981115	0.0
26	3.197234520029555E-5	0.0	0.0	11.217369424142893	0.0
27	3.197234520029555E-5	0.0	0.0	11.495432910349864	0.0
28	3.197234520029555E-5	0.0	0.0	11.76006801157271	0.0
29	6.39446904005911E-5	0.0	0.0	12.036468935829266	0.0
30	9.591703560088666E-5	0.0	0.0	12.300880230635709	0.0
31	9.591703560088666E-5	0.0	0.0	12.54984888271041	0.0
32	9.591703560088666E-5	0.0	0.0	12.83424289326704	0.0
33	9.591703560088666E-5	0.0	0.0	13.125159262244528	0.0
34	9.591703560088666E-5	0.0	0.0	13.490507250848307	0.0
35	1.278893808011822E-4	0.0	0.0	13.759362701637592	0.0
36	1.278893808011822E-4	0.0	0.0	14.049735540746676	0.0
37	1.278893808011822E-4	0.0	0.0	14.390049183058622	0.0
38	1.278893808011822E-4	0.0	0.0	14.936392617841271	0.0
39	1.278893808011822E-4	0.0	0.0	15.305353481452682	0.0
40	1.278893808011822E-4	0.0	0.0	15.674602096170895	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	20	6.3131296E-4	46.0	31
CGTTTTT	14285	0.0	43.32727	1
TACGGGA	985	0.0	42.26396	3
CGCACTT	8450	0.0	42.21657	34
CCGCACT	8485	0.0	42.150856	33
CGACGGT	620	0.0	41.919357	27
CCCGCAC	8625	0.0	41.81333	32
CAACGCA	9200	0.0	41.475	16
TATGACC	9020	0.0	41.4102	27
ACCCGCA	8710	0.0	41.405277	31
AACGCAA	9225	0.0	41.33767	17
GCACTTA	8685	0.0	41.100746	35
ACGCAAG	9285	0.0	41.095318	18
TCAACGC	9325	0.0	41.04236	15
CTTAATC	9710	0.0	41.02575	10
AGCTTAT	9225	0.0	40.839024	23
CGCAAGC	9335	0.0	40.825924	19
CGGTCTA	620	0.0	40.806454	30
CTAGCGG	825	0.0	40.70303	1
GGACTTA	9955	0.0	40.68609	7
>>END_MODULE