FastQCFastQC Report
Sat 14 Jan 2017
SRR2933038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911880
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196432.154121156292495No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC182632.002785454226433No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG69130.7581041365091898No Hit
GGGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT29890.32778435759091107No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT22640.248278282230118Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG21830.23939553450015352No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGCGTCTAATCG21600.23687327279905251No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA19900.2182304689213493No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT18000.19739439399921044No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT17860.19585910426810543No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG14100.15462560863271482No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGCGTCTAAT13330.14618151511163752No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC12720.1394920384261087No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC10550.11569504759398165No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG10160.11141816905733211No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG10070.11043119708733606No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT9450.10363205684958546No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG9320.10220643067070229No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAG206.3107646E-446.01
CGACGGT206.3107646E-446.028
CCGATAG206.3107646E-446.011
ATCGTAG253.416596E-546.01
TGTTGCG551.8189894E-1246.01
GCCGACG206.3107646E-446.01
AGTTGCG253.416596E-546.01
CGAATCT206.3107646E-446.015
CGGGACG206.3107646E-446.06
CATACGG405.6097633E-946.02
TCGTACT206.3107646E-446.015
TATAGCG206.3107646E-446.01
CCGCGTC206.3107646E-446.018
CGACTTA206.3107646E-446.017
ATACGAG206.3107646E-446.01
CGCAAGA206.3107646E-446.045
GATTCCG206.3107646E-446.09
ATACCGG206.3107646E-446.02
ACAACGA1300.045.99999613
CGTGCGG453.092282E-1045.9999962
Produced by FastQC (version 0.11.5)