##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933038.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	911880
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44772009474931	33.0	31.0	34.0	31.0	34.0
2	32.815246523665394	34.0	31.0	34.0	31.0	34.0
3	32.81814164144405	34.0	31.0	34.0	31.0	34.0
4	36.28852699916656	37.0	37.0	37.0	35.0	37.0
5	36.13659253410537	37.0	35.0	37.0	35.0	37.0
6	36.24244637452296	37.0	37.0	37.0	35.0	37.0
7	36.52564262841602	37.0	37.0	37.0	35.0	37.0
8	36.49701824801509	37.0	37.0	37.0	35.0	37.0
9	38.305506206957055	39.0	39.0	39.0	37.0	39.0
10	37.968444312848185	39.0	38.0	39.0	35.0	39.0
11	37.676540772908716	39.0	37.0	39.0	35.0	39.0
12	37.41900579023556	39.0	37.0	39.0	35.0	39.0
13	37.37713185945519	39.0	37.0	39.0	35.0	39.0
14	38.131262885467386	41.0	38.0	41.0	33.0	41.0
15	38.441633767601	41.0	38.0	41.0	33.0	41.0
16	38.685480545685834	41.0	38.0	41.0	34.0	41.0
17	38.735733868491465	41.0	38.0	41.0	35.0	41.0
18	38.671660744834846	40.0	38.0	41.0	35.0	41.0
19	38.610668070360134	40.0	37.0	41.0	35.0	41.0
20	38.436490547001796	40.0	36.0	41.0	34.0	41.0
21	38.39819274465938	40.0	36.0	41.0	35.0	41.0
22	38.32965302452077	40.0	35.0	41.0	34.0	41.0
23	38.32873514058868	40.0	35.0	41.0	35.0	41.0
24	38.28675044962056	40.0	35.0	41.0	34.0	41.0
25	38.126333508795014	40.0	35.0	41.0	34.0	41.0
26	38.043029784620785	40.0	35.0	41.0	34.0	41.0
27	37.93292319164802	40.0	35.0	41.0	34.0	41.0
28	37.91881826556126	40.0	35.0	41.0	34.0	41.0
29	37.867285169101194	40.0	35.0	41.0	34.0	41.0
30	37.8192744659385	40.0	35.0	41.0	34.0	41.0
31	37.7010067114094	40.0	35.0	41.0	34.0	41.0
32	37.59171491862964	40.0	35.0	41.0	34.0	41.0
33	37.49152958722639	40.0	35.0	41.0	33.0	41.0
34	37.31919002500329	40.0	35.0	41.0	33.0	41.0
35	37.22507457121551	40.0	35.0	41.0	33.0	41.0
36	37.14549940781682	40.0	35.0	41.0	33.0	41.0
37	37.04717835680133	40.0	35.0	41.0	32.0	41.0
38	36.99720686932491	40.0	35.0	41.0	32.0	41.0
39	36.956726762293286	40.0	35.0	41.0	32.0	41.0
40	36.89838904241786	40.0	35.0	41.0	32.0	41.0
41	36.75414857218055	40.0	35.0	41.0	32.0	41.0
42	36.789553450015354	40.0	35.0	41.0	32.0	41.0
43	36.75672456902224	40.0	35.0	41.0	32.0	41.0
44	36.724561345791116	39.0	35.0	41.0	32.0	41.0
45	36.64607843137255	39.0	35.0	41.0	32.0	41.0
46	36.592011010220645	39.0	35.0	41.0	32.0	41.0
47	36.47426306092907	39.0	35.0	41.0	31.0	41.0
48	36.35086634206255	39.0	35.0	41.0	31.0	41.0
49	36.29398166425407	39.0	35.0	41.0	31.0	41.0
50	36.129500592183184	38.0	35.0	41.0	31.0	41.0
51	35.99578891959468	38.0	35.0	40.0	31.0	41.0
52	35.75351800675527	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	3.0
15	16.0
16	45.0
17	93.0
18	196.0
19	320.0
20	593.0
21	905.0
22	1468.0
23	2431.0
24	3999.0
25	6616.0
26	8605.0
27	9166.0
28	9560.0
29	10451.0
30	11965.0
31	14486.0
32	18085.0
33	24900.0
34	61668.0
35	81022.0
36	61580.0
37	64186.0
38	102233.0
39	414621.0
40	2662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.481905513883405	22.442645962188006	25.65523972452516	14.420208799403431
2	38.6719743825942	22.29141992367417	25.912620081589683	13.123985612141947
3	32.06342939860508	23.336513576347766	31.609312628854674	12.990744396192481
4	28.741939728911696	26.095209896038952	30.800324604114575	14.362525770934772
5	24.110299600824668	30.816883800500065	30.378339255165155	14.694477343510112
6	25.407400096503924	37.06167478176953	28.407356231083035	9.123568890643506
7	86.89827608895907	1.2558670000438654	9.811378690178532	2.034478220818529
8	85.18390577707594	1.501952011229548	10.071610299600826	3.242531912093696
9	80.39303417116287	3.653112251612054	11.772820985217352	4.18103259200772
10	42.867482563495194	32.22825371759442	16.120103522393297	8.784160196517085
11	24.733956222309953	23.904570776856605	35.60271088301092	15.758762117822519
12	26.520375488002806	18.812892047199192	36.17647058823529	18.490261876562705
13	27.28462078343642	20.161315085318243	38.046014826512256	14.508049304733078
14	16.59779795587139	23.223340790454884	41.29117866385928	18.88768258981445
15	13.434223801377373	30.303000394788786	40.2738298898978	15.988945913936044
16	27.516559196385487	27.389678466464883	30.972386717550553	14.12137561959907
17	24.017195244988375	24.536232837654076	29.174672105978853	22.271899811378688
18	20.572663069702156	24.67342194148353	36.87886564021582	17.8750493485985
19	19.699192876255648	27.47971224283897	30.90329867965083	21.917796201254554
20	15.688029126639469	38.69774531736632	28.684147036890817	16.930078519103393
21	21.04838355924025	27.89928499363951	36.2998420844848	14.752489362635435
22	17.14161951133921	24.21601526516647	38.873426328025616	19.7689388954687
23	17.939092863096022	25.69274465938501	37.33506601745844	19.033096460060534
24	19.96490766328903	29.727157082072203	31.549217002237135	18.758718252401632
25	21.88072992060359	27.56360486028863	31.944334780892227	18.611330438215553
26	16.855945957801467	31.58441900250033	27.77064964688336	23.788985392814844
27	20.858665613896566	26.41893670219766	33.033622845111196	19.68877483879458
28	16.213646532438478	30.085098916524107	29.280497433872878	24.42075711716454
29	21.45402903890863	29.11567311488354	28.389700399175332	21.040597447032503
30	23.975303768039655	24.872899942974954	28.821007150063604	22.330789138921787
31	21.887309733736895	26.41794973022766	31.612492871869108	20.08224766416634
32	24.90481203667149	25.665986752642894	28.199982453831645	21.22921875685397
33	22.92428828354608	25.651620827301834	30.0810413650919	21.343049524060184
34	18.10753607930868	25.482190639119185	35.54261525639338	20.867658025178752
35	19.57077685660394	30.577597929552137	29.641290520682546	20.21033469316138
36	22.347896653068386	27.916721498442776	26.730490854059745	23.004890994429093
37	18.034061499320085	30.80306619292012	29.62166074483485	21.541211562924946
38	20.47078562968812	27.94238276966268	31.75659077948853	19.83024082116068
39	18.160722902136246	29.879260429003818	28.022108172127915	23.937908496732028
40	23.57601877440014	26.99916655700311	30.82565688467781	18.599157783918937
41	17.421919550818092	28.80664122472255	27.444510242575777	26.32692898188358
42	17.304140895731894	30.746041145764792	31.413892178795454	20.535925779707856
43	18.383230249594245	28.907970346975482	31.835548537088215	20.87325086634206
44	22.99864017195245	26.74112821862526	27.833048208097555	22.427183401324736
45	18.697306663157434	25.191911216388117	27.600561477387377	28.51022064306707
46	21.9492696407422	25.362438040092993	29.191340965916567	23.496951353248235
47	17.008378295389743	26.80900995745054	33.26753520200027	22.91507654515945
48	17.083826819318332	32.06968022108172	29.32973636882046	21.516756590779487
49	22.621068561652848	27.3389042417862	29.822016054744044	20.218011141816906
50	19.521757248760803	26.30225468263368	29.967429924990128	24.208558143615388
51	19.544786594727377	26.58507698381366	27.798942843356585	26.07119357810238
52	18.622406456989953	27.977146115716977	30.054722112558668	23.345725314734395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	367.0
1	936.5
2	1506.0
3	11984.5
4	22463.0
5	12670.0
6	2877.0
7	3305.5
8	3734.0
9	5067.5
10	6401.0
11	7879.5
12	9358.0
13	9703.0
14	9644.0
15	9240.0
16	8433.0
17	7626.0
18	6863.0
19	6100.0
20	5859.5
21	5619.0
22	5915.5
23	6212.0
24	6756.0
25	7300.0
26	8050.0
27	8800.0
28	10437.5
29	12075.0
30	13818.5
31	15562.0
32	16586.5
33	17611.0
34	20959.0
35	24307.0
36	25532.5
37	26758.0
38	27594.5
39	31147.5
40	33864.0
41	37435.0
42	41006.0
43	45617.0
44	50228.0
45	57477.5
46	64727.0
47	73052.5
48	81378.0
49	88423.5
50	95469.0
51	89147.0
52	82825.0
53	73111.5
54	63398.0
55	55082.0
56	46766.0
57	42161.0
58	37556.0
59	40995.5
60	44435.0
61	29990.0
62	15545.0
63	12556.0
64	7697.5
65	5828.0
66	4341.5
67	2855.0
68	2150.5
69	1446.0
70	1187.5
71	929.0
72	775.5
73	622.0
74	531.5
75	441.0
76	424.0
77	407.0
78	257.5
79	108.0
80	73.5
81	39.0
82	35.5
83	32.0
84	21.0
85	10.0
86	5.5
87	1.0
88	0.5
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	911880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.969153668205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54783953604111	21.14259044015484
2	11.794486440217934	7.069415531289029
3	4.62602341452413	4.15914019747764
4	2.4194120230048672	2.900309228165424
5	1.4195249975812425	2.1270981394185293
6	0.9671170862290788	1.7390208343407556
7	0.7272267795745914	1.5256059776093343
8	0.5652785484658904	1.355273574745131
9	0.4900303418573633	1.3217215155485715
>10	6.123798418187076	40.9082874123394
>50	0.2690454857239297	4.842892359512129
>100	0.03775433317558404	2.151102496032552
>500	0.006597844632626229	1.3882970761341615
>1k	0.004765110012452276	2.445832758357897
>5k	3.6654692403479045E-4	0.7594000385595393
>10k+	7.330938480695809E-4	4.164012420315044
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19643	2.154121156292495	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	18263	2.002785454226433	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	6913	0.7581041365091898	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT	2989	0.32778435759091107	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT	2264	0.248278282230118	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	2183	0.23939553450015352	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGCGTCTAATCG	2160	0.23687327279905251	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1990	0.2182304689213493	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	1800	0.19739439399921044	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	1786	0.19585910426810543	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1410	0.15462560863271482	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGCGTCTAAT	1333	0.14618151511163752	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1272	0.1394920384261087	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC	1055	0.11569504759398165	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1016	0.11141816905733211	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	1007	0.11043119708733606	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT	945	0.10363205684958546	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	932	0.10220643067070229	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25683203930341714	0.0
2	0.0	0.0	0.0	0.7788305478791069	0.0
3	0.0	0.0	0.0	1.2878887572926263	0.0
4	0.0	0.0	0.0	1.831600649208229	0.0
5	0.0	0.0	0.0	3.5710839145501603	0.0
6	0.0	0.0	0.0	4.845813045576172	0.0
7	0.0	0.0	0.0	5.699982453831645	0.0
8	0.0	0.0	0.0	7.353379830679476	0.0
9	0.0	0.0	0.0	8.176075799447295	0.0
10	0.0	0.0	0.0	9.373053471948063	0.0
11	0.0	0.0	0.0	11.267381673027153	0.0
12	0.0	0.0	0.0	12.4471421678291	0.0
13	0.0	0.0	0.0	13.000833442996885	0.0
14	0.0	0.0	0.0	13.468767820327235	0.0
15	0.0	0.0	0.0	13.91115058998991	0.0
16	0.0	0.0	0.0	14.540400052638505	0.0
17	0.0	0.0	0.0	15.348401105408607	0.0
18	0.0	0.0	0.0	16.291397990963723	0.0
19	0.0	0.0	0.0	16.844431284818178	0.0
20	0.0	0.0	0.0	17.525661271219896	0.0
21	0.0	0.0	0.0	18.149756546914066	0.0
22	0.0	0.0	0.0	18.811247093915867	0.0
23	0.0	0.0	0.0	19.456617098741063	0.0
24	0.0	0.0	0.0	19.919616616221433	0.0
25	0.0	0.0	0.0	20.465192788524806	0.0
26	0.0	0.0	0.0	20.91174277317191	0.0
27	0.0	0.0	0.0	21.596043339035838	0.0
28	0.0	0.0	0.0	22.101372987673816	0.0
29	0.0	0.0	0.0	22.713514936175812	0.0
30	0.0	0.0	0.0	23.978374347501866	0.0
31	1.0966355222178357E-4	0.0	0.0	24.541496688160723	0.0
32	1.0966355222178357E-4	0.0	0.0	24.96633328946791	0.0
33	1.0966355222178357E-4	0.0	0.0	25.487344826073606	0.0
34	1.0966355222178357E-4	0.0	0.0	26.034456288108085	0.0
35	1.0966355222178357E-4	0.0	0.0	26.560183357459316	0.0
36	1.0966355222178357E-4	0.0	0.0	26.976466201693206	0.0
37	1.0966355222178357E-4	0.0	0.0	27.475764354958987	0.0
38	1.0966355222178357E-4	0.0	0.0	28.03855770496118	0.0
39	1.0966355222178357E-4	0.0	0.0	29.54248366013072	0.0
40	1.0966355222178357E-4	0.0	0.0	30.026757906742116	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAG	20	6.3107646E-4	46.0	1
CGACGGT	20	6.3107646E-4	46.0	28
CCGATAG	20	6.3107646E-4	46.0	11
ATCGTAG	25	3.416596E-5	46.0	1
TGTTGCG	55	1.8189894E-12	46.0	1
GCCGACG	20	6.3107646E-4	46.0	1
AGTTGCG	25	3.416596E-5	46.0	1
CGAATCT	20	6.3107646E-4	46.0	15
CGGGACG	20	6.3107646E-4	46.0	6
CATACGG	40	5.6097633E-9	46.0	2
TCGTACT	20	6.3107646E-4	46.0	15
TATAGCG	20	6.3107646E-4	46.0	1
CCGCGTC	20	6.3107646E-4	46.0	18
CGACTTA	20	6.3107646E-4	46.0	17
ATACGAG	20	6.3107646E-4	46.0	1
CGCAAGA	20	6.3107646E-4	46.0	45
GATTCCG	20	6.3107646E-4	46.0	9
ATACCGG	20	6.3107646E-4	46.0	2
ACAACGA	130	0.0	45.999996	13
CGTGCGG	45	3.092282E-10	45.999996	2
>>END_MODULE