FastQCFastQC Report
Sat 14 Jan 2017
SRR2933037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834236
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399862.179981201982733No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC236641.2901284240413993No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG120180.6552046737715321No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT29940.16322872302146504No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC29140.15886723409637582No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA28120.15330633571688704No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT24190.1318805213723861No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA23880.13019044441391403No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT22750.12402984130722547No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCTT22240.12124939211748108Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC21380.11656079152301013No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG20930.11410745400264742No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT20520.11187219092853919No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA20450.11149056064759387No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG19430.10592966226810507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA253.4179582E-546.015
CGAACCG206.3124445E-446.015
GCACGAC253.4179582E-546.031
ACTTACG206.3124445E-446.01
CGTTACA253.4179582E-546.022
CCCGTAA253.4179582E-546.031
CGAGAGT301.862014E-646.022
GTCCCGT453.110472E-1046.027
ATTTGCG501.6370905E-1146.01
TATCGAG253.4179582E-546.01
TCGAATT301.862014E-646.038
TCGCGAT206.3124445E-446.045
GTACGCC206.3124445E-446.021
TCGCATA351.0200165E-745.99999641
ACCGGAT351.0200165E-745.99999629
GTACGTC351.0200165E-745.99999646
CGTTTTT91450.043.761621
CGTGACG950.043.57894519
TCGACAG700.042.7142831
GCGAGAC3250.042.46153621
Produced by FastQC (version 0.11.5)