##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933037.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1834236
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48969816315894	33.0	31.0	34.0	31.0	34.0
2	32.87966597537067	34.0	31.0	34.0	31.0	34.0
3	32.89621346435246	34.0	31.0	34.0	31.0	34.0
4	36.35722611485109	37.0	37.0	37.0	35.0	37.0
5	36.25051629125151	37.0	37.0	37.0	35.0	37.0
6	36.33563619948578	37.0	37.0	37.0	35.0	37.0
7	36.53125170370661	37.0	37.0	37.0	35.0	37.0
8	36.488610516858245	37.0	37.0	37.0	35.0	37.0
9	38.319738027167716	39.0	39.0	39.0	37.0	39.0
10	38.01897847387141	39.0	39.0	39.0	35.0	39.0
11	37.68763288911568	39.0	37.0	39.0	35.0	39.0
12	37.32608617429818	39.0	37.0	39.0	35.0	39.0
13	37.23459194999989	39.0	37.0	39.0	35.0	39.0
14	38.043837325186075	40.0	37.0	41.0	33.0	41.0
15	38.34031117042736	41.0	37.0	41.0	33.0	41.0
16	38.57679600662074	41.0	37.0	41.0	34.0	41.0
17	38.628883633294734	41.0	37.0	41.0	35.0	41.0
18	38.54201585837373	40.0	37.0	41.0	35.0	41.0
19	38.46845880246599	40.0	37.0	41.0	35.0	41.0
20	38.27152067672862	40.0	35.0	41.0	34.0	41.0
21	38.229658560839496	40.0	35.0	41.0	34.0	41.0
22	38.184188403237094	40.0	35.0	41.0	34.0	41.0
23	38.15836675324222	40.0	35.0	41.0	34.0	41.0
24	38.08942578817557	40.0	35.0	41.0	34.0	41.0
25	37.95303603244076	40.0	35.0	41.0	34.0	41.0
26	37.85249389936737	40.0	35.0	41.0	34.0	41.0
27	37.73398352229484	40.0	35.0	41.0	34.0	41.0
28	37.69065267501019	40.0	35.0	41.0	34.0	41.0
29	37.64259288335852	40.0	35.0	41.0	34.0	41.0
30	37.58916137290949	40.0	35.0	41.0	34.0	41.0
31	37.462329274967885	40.0	35.0	41.0	34.0	41.0
32	37.35314921307836	40.0	35.0	41.0	33.0	41.0
33	37.23252024276047	40.0	35.0	41.0	33.0	41.0
34	37.03344716819428	40.0	35.0	41.0	33.0	41.0
35	36.94017945346182	40.0	35.0	41.0	33.0	41.0
36	36.83644798161196	39.0	35.0	41.0	33.0	41.0
37	36.71726757080332	39.0	35.0	41.0	32.0	41.0
38	36.6737006579306	39.0	35.0	41.0	32.0	41.0
39	36.63264160118982	39.0	35.0	41.0	32.0	41.0
40	36.56987813999943	39.0	35.0	41.0	32.0	41.0
41	36.44333662625747	39.0	35.0	41.0	31.0	41.0
42	36.476082685107045	39.0	35.0	41.0	32.0	41.0
43	36.43877887033075	39.0	35.0	41.0	32.0	41.0
44	36.406013729967135	39.0	35.0	41.0	32.0	41.0
45	36.348823161250785	39.0	35.0	41.0	32.0	41.0
46	36.30616834474953	38.0	35.0	41.0	32.0	41.0
47	36.22891710772223	38.0	35.0	41.0	31.0	41.0
48	36.11718666518376	38.0	35.0	41.0	31.0	41.0
49	36.092946600110345	38.0	35.0	41.0	31.0	41.0
50	35.99152998850748	38.0	35.0	41.0	31.0	41.0
51	35.923108585809025	38.0	35.0	41.0	31.0	41.0
52	35.68501599576063	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	5.0
13	8.0
14	13.0
15	41.0
16	101.0
17	218.0
18	419.0
19	771.0
20	1303.0
21	2204.0
22	3471.0
23	5332.0
24	8463.0
25	13310.0
26	17313.0
27	18923.0
28	19846.0
29	20722.0
30	23682.0
31	28100.0
32	36105.0
33	51165.0
34	138843.0
35	192115.0
36	135149.0
37	137021.0
38	204304.0
39	769837.0
40	5448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.77231283215464	23.07413004651528	25.371707893640732	14.781849227689348
2	38.19257718199839	23.3459053251599	25.525559415473253	12.93595807736845
3	31.65677699052903	23.452434692155208	31.38930868219793	13.501479635117835
4	27.665142326287345	27.36060136209299	31.031339478671228	13.942916832948432
5	24.91086207009349	31.332609326171767	29.307733574087525	14.448795029647222
6	27.169840740231898	38.2656866401052	25.436748597236125	9.127724022426777
7	86.46733571906778	1.5938516090623016	9.651266249272176	2.2875464225977464
8	84.6681670188569	2.0754145050037183	10.044236401422719	3.212182074716667
9	79.30784261131065	4.263355424274739	12.183328644732738	4.245473319681873
10	46.56423709926095	24.48458104627758	17.187592000157014	11.763589854304463
11	30.951960380234606	23.547079001829644	30.572292769305587	14.928667848630164
12	30.35389121138174	19.68318144448152	31.724271031644786	18.23865631249196
13	28.401579731288667	21.590951218927117	34.24341251616477	15.764056533619447
14	17.73866612584204	23.54162714067328	38.63995690848942	20.079749824995258
15	14.88985059719687	29.534312923745908	38.813762242154226	16.762074236902993
16	27.207022433318283	27.09705839379447	31.53285618644493	14.163062986442313
17	25.234375511111985	26.042995557823527	28.623688554798836	20.098940376265652
18	22.221786073329714	26.23015795132142	33.58324664874095	17.964809326607917
19	21.45274653861335	28.47190874020573	28.82606164092298	21.24928308025794
20	17.274658222824108	37.48607049474549	27.77467021691865	17.464601065511744
21	22.023338327238154	29.64215073741874	33.302584836411455	15.031926098931653
22	19.740044356342366	24.87771475426281	35.784053960340984	19.59818692905384
23	19.925789265939606	26.436565414701267	34.32366391238641	19.313981406972715
24	19.836814891867785	30.58968420639438	31.035428374538498	18.538072527199333
25	22.44067829875763	27.494444553481667	31.043551647661477	19.021325500099223
26	18.420912030949125	31.022943612490433	27.284929529242692	23.27121482731775
27	21.016379571658174	27.48981047149876	32.33253518086004	19.161274775983024
28	16.974097117273896	31.14441107905417	27.82564511873063	24.055846684941308
29	20.574724299381323	30.395925060897287	27.24436768223936	21.784982957482026
30	23.8349917894971	26.852815013989478	27.475635632492217	21.836557564021206
31	21.366389057896583	27.553924358697575	31.157768138887253	19.921918444518592
32	25.387463772382617	27.725167317618887	25.75361076764386	21.133758142354637
33	24.76082685107042	27.552888505077867	28.513833552498152	19.172451091353565
34	19.502070616867186	28.057894403991636	32.598422449455796	19.841612529685385
35	21.294588046467304	32.68030940402434	26.804838635813493	19.220263913694858
36	24.36545788001108	30.298391264809982	25.86799081470432	19.468160040474615
37	20.32933602873349	33.760595692157395	27.267647129377025	18.642421149732098
38	23.092284744165966	30.25068747969182	29.06087330092747	17.59615447521475
39	20.458054470635183	30.081025560505847	28.03750444326684	21.423415525592127
40	24.68139323402223	28.17363741634119	28.89786265235226	18.24710669728432
41	19.063250312391645	28.715007229167895	27.70875721553824	24.51298524290222
42	18.52984021685323	29.38345992554938	31.739263649824778	20.34743620777261
43	18.783842428128114	29.65861535811095	31.580559971563094	19.976982242197842
44	22.682686415488522	28.373938795225918	27.761476712920256	21.18189807636531
45	19.419256845902055	28.104998484382598	27.111505825858835	25.36423884385652
46	21.522911991695725	27.18881321705604	28.556303550906208	22.731971240342027
47	18.458148242647074	27.429131256828455	31.568184246738152	22.54453625378632
48	18.89522395155258	31.588247095793566	28.92452225340687	20.59200669924699
49	22.985700858559095	28.10069151406907	28.442359652738254	20.47124797463358
50	20.219317470598114	28.51154377081248	29.06027359620027	22.208865162389134
51	19.727941224575247	28.317402995034445	28.41297412110546	23.541681659284848
52	19.159366624578297	29.827514016735034	28.923758992844977	22.08936036584169
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1774.0
1	3384.5
2	4995.0
3	25558.0
4	46121.0
5	26353.0
6	6585.0
7	7483.5
8	8382.0
9	11862.0
10	15342.0
11	19192.0
12	23042.0
13	24272.0
14	23600.5
15	21699.0
16	19082.0
17	16465.0
18	14438.0
19	12411.0
20	11779.5
21	11148.0
22	11161.0
23	11174.0
24	12709.0
25	14244.0
26	15138.5
27	16033.0
28	18738.5
29	21444.0
30	24469.0
31	27494.0
32	30863.5
33	34233.0
34	38603.5
35	42974.0
36	47350.5
37	51727.0
38	53190.5
39	58181.0
40	61708.0
41	72955.5
42	84203.0
43	96601.5
44	109000.0
45	123654.0
46	138308.0
47	145941.5
48	153575.0
49	158529.0
50	163483.0
51	143725.0
52	123967.0
53	116002.0
54	108037.0
55	99191.5
56	90346.0
57	84956.5
58	79567.0
59	84474.5
60	89382.0
61	69547.5
62	49713.0
63	42281.0
64	30821.0
65	26793.0
66	22704.0
67	18615.0
68	15563.5
69	12512.0
70	10893.5
71	9275.0
72	7104.5
73	4934.0
74	4357.0
75	3780.0
76	3111.5
77	2443.0
78	1871.0
79	1299.0
80	975.0
81	651.0
82	411.5
83	172.0
84	113.5
85	55.0
86	47.5
87	40.0
88	53.5
89	37.5
90	8.0
91	7.0
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1834236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.833547681954446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.33126715300911	13.949185406114829
2	10.983442089452963	4.356812447863015
3	4.92466151288991	2.930205268001638
4	2.762918967732152	2.19193940351568
5	1.7560485457651973	1.7414336282130405
6	1.1929694407236564	1.4196489771424299
7	0.8282923260660385	1.1499582740539416
8	0.6100432765481533	0.9679457930778815
9	0.482121407187889	0.8605960126156775
>10	3.9764361042983274	17.23535468545572
>50	1.1387906242447488	16.435722639725363
>100	1.0005976114903594	29.624730210086465
>500	0.00524017491641633	0.7032864199043743
>1k	0.006343369635661873	2.294091091574205
>5k	0.0	0.0
>10k+	8.273960394341573E-4	4.13908974265569
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39986	2.179981201982733	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	23664	1.2901284240413993	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	12018	0.6552046737715321	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	2994	0.16322872302146504	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	2914	0.15886723409637582	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	2812	0.15330633571688704	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT	2419	0.1318805213723861	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2388	0.13019044441391403	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	2275	0.12402984130722547	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCTT	2224	0.12124939211748108	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	2138	0.11656079152301013	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2093	0.11410745400264742	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	2052	0.11187219092853919	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA	2045	0.11149056064759387	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	1943	0.10592966226810507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12615606715820646	0.0
2	0.0	0.0	0.0	0.3769416803508382	0.0
3	0.0	0.0	0.0	0.6531329665321147	0.0
4	0.0	0.0	0.0	0.9939833260278393	0.0
5	0.0	0.0	0.0	2.045810898924675	0.0
6	0.0	0.0	0.0	3.2822930091874762	0.0
7	0.0	0.0	0.0	4.352984021685323	0.0
8	0.0	0.0	0.0	6.07326429096365	0.0
9	0.0	0.0	0.0	7.156712658567382	0.0
10	0.0	0.0	0.0	8.169995573088741	0.0
11	5.451861156361559E-5	0.0	0.0	9.458597476006359	0.0
12	5.451861156361559E-5	0.0	0.0	10.170610543027179	0.0
13	5.451861156361559E-5	0.0	0.0	10.496849914623855	0.0
14	5.451861156361559E-5	0.0	0.0	10.84963985005201	0.0
15	5.451861156361559E-5	0.0	0.0	11.083252100602104	0.0
16	5.451861156361559E-5	0.0	0.0	11.408564655802198	0.0
17	5.451861156361559E-5	0.0	0.0	11.782071663624528	0.0
18	5.451861156361559E-5	0.0	0.0	12.207807501324803	0.0
19	5.451861156361559E-5	0.0	0.0	12.477620109953136	0.0
20	1.0903722312723118E-4	0.0	0.0	12.837279390438308	0.0
21	1.0903722312723118E-4	0.0	0.0	13.171969146827344	0.0
22	1.0903722312723118E-4	0.0	0.0	13.52972027590779	0.0
23	1.0903722312723118E-4	0.0	0.0	13.863701290346498	0.0
24	1.6355583469084676E-4	0.0	0.0	14.11132482406844	0.0
25	1.6355583469084676E-4	0.0	0.0	14.390078484993207	0.0
26	1.6355583469084676E-4	0.0	0.0	14.622981993592973	0.0
27	2.1807444625446235E-4	0.0	0.0	14.959525382775173	0.0
28	2.1807444625446235E-4	0.0	0.0	15.212437221818785	0.0
29	2.725930578180779E-4	0.0	0.0	15.552360764917928	0.0
30	2.725930578180779E-4	0.0	0.0	16.49286133300186	0.0
31	2.725930578180779E-4	0.0	0.0	16.881524514838876	0.0
32	2.725930578180779E-4	0.0	0.0	17.236604231952704	0.0
33	2.725930578180779E-4	0.0	0.0	17.56044478464058	0.0
34	2.725930578180779E-4	0.0	0.0	17.907891896135503	0.0
35	2.725930578180779E-4	0.0	0.0	18.242254540855157	0.0
36	2.725930578180779E-4	0.0	0.0	18.48715214399892	0.0
37	2.725930578180779E-4	0.0	0.0	18.796163634341493	0.0
38	2.725930578180779E-4	0.0	0.0	19.171360719122294	0.0
39	2.725930578180779E-4	0.0	0.0	20.60574538936102	0.0
40	2.725930578180779E-4	0.0	0.0	21.01981424418668	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	25	3.4179582E-5	46.0	15
CGAACCG	20	6.3124445E-4	46.0	15
GCACGAC	25	3.4179582E-5	46.0	31
ACTTACG	20	6.3124445E-4	46.0	1
CGTTACA	25	3.4179582E-5	46.0	22
CCCGTAA	25	3.4179582E-5	46.0	31
CGAGAGT	30	1.862014E-6	46.0	22
GTCCCGT	45	3.110472E-10	46.0	27
ATTTGCG	50	1.6370905E-11	46.0	1
TATCGAG	25	3.4179582E-5	46.0	1
TCGAATT	30	1.862014E-6	46.0	38
TCGCGAT	20	6.3124445E-4	46.0	45
GTACGCC	20	6.3124445E-4	46.0	21
TCGCATA	35	1.0200165E-7	45.999996	41
ACCGGAT	35	1.0200165E-7	45.999996	29
GTACGTC	35	1.0200165E-7	45.999996	46
CGTTTTT	9145	0.0	43.76162	1
CGTGACG	95	0.0	43.578945	19
TCGACAG	70	0.0	42.714283	1
GCGAGAC	325	0.0	42.461536	21
>>END_MODULE