FastQCFastQC Report
Sat 14 Jan 2017
SRR2933036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476294
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC111412.339101479338392No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86061.8068671870735302No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG47350.9941338752955107No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT18720.3930345542878978Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGAATCACGTCG16820.35314322666252357No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG15780.33130797364652925No Hit
GGGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT12850.2697913473610837No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT12340.25908367520900955No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT11620.24396696158255196No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGAATCACGT10600.2225516172784037No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA10210.21436339739740579No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG8010.1681734390943409No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC7610.15977526485742No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC7470.1568359038744977No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG7440.15620604080672862No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCG6510.13668028570588753No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC6400.1343707877907343No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCGAAT6350.1333210160111192No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG5560.11673462189320041No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.11547489575766227No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG5170.10854640201220256No Hit
AGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG5050.10602694974112628No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT206.3077116E-446.00000431
ACTGATC206.3077116E-446.00000416
ATGTGCG206.3077116E-446.0000041
GTAGGGT405.5988494E-946.0000044
TACGGGA405.5988494E-946.0000044
AGTGCGA206.3077116E-446.00000429
CTCATGC206.3077116E-446.00000423
TCGAGTC206.3077116E-446.00000416
CGGTATG206.3077116E-446.00000431
CACGTAA206.3077116E-446.00000433
TAGTGCG206.3077116E-446.0000041
TCGGTAT206.3077116E-446.00000430
GTTGCGC206.3077116E-446.00000441
TACCGGG206.3077116E-446.0000043
TTTAGCG301.8590908E-646.01
GCGACTC301.8590908E-646.043
CTCGTTT253.4141216E-546.022
CTCGTAA600.046.033
GGTCGCT253.4141216E-546.010
CGAAAGG501.6370905E-1146.02
Produced by FastQC (version 0.11.5)