##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933036.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476294
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3829924374441	33.0	31.0	34.0	31.0	34.0
2	32.7486405455454	34.0	31.0	34.0	31.0	34.0
3	32.765823210034135	34.0	31.0	34.0	31.0	34.0
4	36.244745892243024	37.0	37.0	37.0	35.0	37.0
5	36.06345870407773	37.0	35.0	37.0	35.0	37.0
6	36.179401798049106	37.0	36.0	37.0	35.0	37.0
7	36.46026193905445	37.0	37.0	37.0	35.0	37.0
8	36.4037254300915	37.0	37.0	37.0	35.0	37.0
9	38.210764779736884	39.0	39.0	39.0	37.0	39.0
10	37.93353894863257	39.0	38.0	39.0	35.0	39.0
11	37.59994877113716	39.0	37.0	39.0	35.0	39.0
12	37.142147497134125	39.0	35.0	39.0	35.0	39.0
13	37.03232037355079	39.0	35.0	39.0	33.0	39.0
14	37.59780303761962	40.0	36.0	41.0	31.0	41.0
15	37.9619226780098	40.0	36.0	41.0	33.0	41.0
16	38.22252852229925	40.0	36.0	41.0	33.0	41.0
17	38.309334150755625	40.0	36.0	41.0	34.0	41.0
18	38.27369649838125	40.0	36.0	41.0	34.0	41.0
19	38.19954901804348	40.0	36.0	41.0	34.0	41.0
20	38.0155366223383	40.0	35.0	41.0	33.0	41.0
21	37.961527963820664	40.0	35.0	41.0	34.0	41.0
22	37.919862521887744	40.0	35.0	41.0	34.0	41.0
23	37.9290774185692	40.0	35.0	41.0	34.0	41.0
24	37.89212755147031	40.0	35.0	41.0	34.0	41.0
25	37.69803314759371	40.0	35.0	41.0	34.0	41.0
26	37.57739757376746	39.0	35.0	41.0	34.0	41.0
27	37.455548463763975	39.0	35.0	41.0	34.0	41.0
28	37.429535119065115	39.0	35.0	41.0	34.0	41.0
29	37.37206011413119	39.0	35.0	41.0	34.0	41.0
30	37.295783696624355	39.0	35.0	41.0	33.0	41.0
31	37.135246297454934	39.0	35.0	41.0	33.0	41.0
32	37.01036754609548	39.0	35.0	41.0	33.0	41.0
33	36.86393488055697	39.0	35.0	41.0	33.0	41.0
34	36.67812737510865	39.0	35.0	41.0	32.0	41.0
35	36.589640012261334	39.0	35.0	41.0	32.0	41.0
36	36.47538705085515	39.0	35.0	41.0	31.0	41.0
37	36.346972668141944	39.0	35.0	41.0	31.0	41.0
38	36.30705194690674	39.0	35.0	41.0	31.0	41.0
39	36.23572835265613	39.0	35.0	41.0	31.0	41.0
40	36.13108080303342	39.0	35.0	41.0	31.0	41.0
41	35.944284412568706	38.0	35.0	41.0	30.0	41.0
42	36.00083561833657	38.0	35.0	41.0	30.0	41.0
43	35.92725291521623	38.0	35.0	41.0	30.0	41.0
44	35.900815462718406	38.0	35.0	41.0	30.0	41.0
45	35.82721386370603	38.0	35.0	41.0	30.0	41.0
46	35.75288792216572	38.0	35.0	41.0	30.0	41.0
47	35.647318672920505	37.0	35.0	41.0	30.0	41.0
48	35.51578646802185	37.0	35.0	40.0	29.0	41.0
49	35.47631714865188	37.0	35.0	40.0	29.0	41.0
50	35.30975825855459	37.0	35.0	40.0	29.0	41.0
51	35.1787635368071	36.0	35.0	40.0	29.0	41.0
52	34.84301082944568	36.0	35.0	39.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	6.0
15	4.0
16	29.0
17	78.0
18	126.0
19	246.0
20	434.0
21	713.0
22	1088.0
23	1686.0
24	2569.0
25	4124.0
26	5072.0
27	5722.0
28	6169.0
29	6739.0
30	7556.0
31	9180.0
32	11176.0
33	15330.0
34	38800.0
35	55030.0
36	33550.0
37	35034.0
38	53455.0
39	181505.0
40	867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.772900771372306	21.735944605642732	25.003044338160883	14.48811028482408
2	40.68033609493296	21.428361474215503	24.55458183390931	13.336720596942225
3	34.84927376788286	21.799980684199255	29.636527019026065	13.71421852889182
4	31.417989729032907	24.38871789273012	30.109134274208788	14.084158104028186
5	24.93123994843521	28.89580805132964	31.74047961973067	14.43247238050448
6	26.381814593507368	34.64477822521384	29.942220561249982	9.031186620028805
7	83.56057393122735	1.8996670123915063	11.657925566981737	2.881833489399405
8	81.12930248963876	2.280524214035869	12.359173115764632	4.231000180560747
9	74.79099043867863	4.475387050855144	14.954838818040958	5.778783692425267
10	50.177201476399034	18.663682515421147	19.39663317194842	11.762482836231403
11	32.147161207153566	24.360584009036433	27.47588674222224	16.016368041587757
12	31.524016678774036	18.141316077884667	33.61768151603841	16.716985727302884
13	29.823806304509397	19.966029385211655	36.46277299315129	13.747391317127654
14	17.42789117645824	22.50962640721907	42.539062007919476	17.52342040840321
15	14.240364144834913	28.78369242526675	40.996737309308955	15.979206120589385
16	29.30710863458284	24.805897197949168	30.663413773845566	15.223580393622427
17	25.651173434895252	23.149567284072443	27.259003892553757	23.940255388478544
18	22.631609888010345	23.89469529324325	35.92822920297127	17.54546561577513
19	23.1063166867523	24.71939600330888	29.749062553800805	22.425224756138014
20	16.860804461110153	38.504159195790834	27.512838708864695	17.12219763423432
21	21.831053928875864	27.03267309686874	37.09137633478482	14.044896639470581
22	17.868165460828816	23.364560544537618	39.34901552402508	19.41825847060849
23	19.324198919154973	24.17897349116302	36.85748718228657	19.63934040739543
24	21.68156642745867	29.89917991828576	29.240343149399322	19.178910504856244
25	24.150209744401568	25.64361507808203	30.672021902438413	19.534153275077998
26	16.67562471918605	30.161622863189542	27.465808933137936	25.69694348448647
27	22.101265184948794	26.736217546305436	33.10329334402701	18.05922392471877
28	16.2055369162744	30.492930836836074	27.952063221455653	25.34946902543387
29	22.55812586343729	30.34764242253734	26.62221233103923	20.47201938298614
30	25.346739618806875	26.858830890164477	26.567204289787398	21.22722520124125
31	21.812158036842792	28.59515341364787	30.64850701457503	18.944181534934305
32	26.43115386714928	26.195795034159573	25.38201195060194	21.991039148089207
33	24.66585764254851	26.606045845633158	28.58885478297018	20.13924172884815
34	17.861027012727433	26.270748739224093	34.059635435256375	21.8085888127921
35	21.127076973465968	31.170663497755584	27.115395113102416	20.586864415676033
36	25.729066500942693	28.690682645592847	23.543651610139957	22.0365992433245
37	18.349800753316227	34.48983191054265	26.24975330363179	20.910614032509333
38	21.63369683430822	26.922657014365075	30.2004644190353	21.2431817322914
39	19.673982876122732	29.023040391018995	25.671329053063864	25.631647679794412
40	26.316518788815312	26.088718312638832	27.804045400529926	19.79071749801593
41	18.73464708772313	26.361029112270995	24.269253864209922	30.635069935795954
42	19.606797482227364	28.13703300902384	30.545839334528672	21.710330174220125
43	18.88350472607255	27.766463570819706	32.09488257252873	21.255149130579014
44	24.456323195337333	25.311257332655877	27.145418585999405	23.087000886007385
45	19.05923652198012	25.071279503835864	26.118741785535825	29.75074218864819
46	22.625941120400427	24.805057380525476	27.917840661440202	24.6511608376339
47	17.214997459552293	25.97240359945748	32.268724779233	24.543874161757234
48	17.030237626340032	31.96911991333084	28.670527027424235	22.330115432904886
49	25.261288195946204	25.6114920616258	28.603971496596643	20.523248245831358
50	20.325051333840022	25.398598344719858	27.840367504104606	26.435982817335514
51	20.596732270404413	26.084939134232215	25.67048923564017	27.647839359723196
52	19.443033084607407	27.38203714512465	29.00183500107077	24.173094769197174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	529.0
1	1149.0
2	1769.0
3	6093.5
4	10418.0
5	6082.0
6	1746.0
7	1954.5
8	2163.0
9	2894.0
10	3625.0
11	4477.5
12	5330.0
13	5528.5
14	5424.5
15	5122.0
16	4639.5
17	4157.0
18	3968.5
19	3780.0
20	3599.0
21	3418.0
22	3181.0
23	2944.0
24	2888.5
25	2833.0
26	3181.0
27	3529.0
28	3884.0
29	4239.0
30	4762.5
31	5286.0
32	5659.0
33	6032.0
34	6398.0
35	6764.0
36	7977.5
37	9191.0
38	9456.5
39	10985.0
40	12248.0
41	14421.0
42	16594.0
43	20113.5
44	23633.0
45	26118.5
46	28604.0
47	35671.5
48	42739.0
49	48255.5
50	53772.0
51	49276.0
52	44780.0
53	39736.5
54	34693.0
55	31962.5
56	29232.0
57	27091.0
58	24950.0
59	27402.5
60	29855.0
61	20935.5
62	12016.0
63	9987.5
64	7035.0
65	6111.0
66	4962.0
67	3813.0
68	3269.0
69	2725.0
70	2174.5
71	1624.0
72	1393.5
73	1163.0
74	899.0
75	635.0
76	501.5
77	368.0
78	305.5
79	243.0
80	172.5
81	102.0
82	86.5
83	71.0
84	40.0
85	9.0
86	15.0
87	21.0
88	11.5
89	4.5
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	476294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.3552344014299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2474463927469	22.3398038214789
2	11.443112394729845	7.1760294283732415
3	4.448878578680631	4.1848689197409445
4	2.3363151029718483	2.9302283075713254
5	1.421747084214246	2.228960654254359
6	0.9422772416749581	1.772719427031067
7	0.6871025850982806	1.5080983829509516
8	0.5988895792262152	1.5022658509769362
9	0.5046756799592609	1.4241801817641278
>10	6.181313471474938	40.66108588492291
>50	0.12795089267463505	2.592000997853278
>100	0.045553197392016666	2.756259083778826
>500	0.007368899578120343	1.4928161338527752
>1k	0.006029099654825735	3.282851182775436
>5k	6.698999616473038E-4	1.8076791399939471
>10k+	6.698999616473038E-4	2.3401526026809862
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	11141	2.339101479338392	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8606	1.8068671870735302	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	4735	0.9941338752955107	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	1872	0.3930345542878978	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGAATCACGTCG	1682	0.35314322666252357	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	1578	0.33130797364652925	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	1285	0.2697913473610837	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1234	0.25908367520900955	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1162	0.24396696158255196	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGAATCACGT	1060	0.2225516172784037	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1021	0.21436339739740579	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	801	0.1681734390943409	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	761	0.15977526485742	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	747	0.1568359038744977	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	744	0.15620604080672862	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCG	651	0.13668028570588753	No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	640	0.1343707877907343	No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCGAAT	635	0.1333210160111192	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	556	0.11673462189320041	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	550	0.11547489575766227	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	517	0.10854640201220256	No Hit
AGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	505	0.10602694974112628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41319017245650796	0.0
2	0.0	0.0	0.0	1.1133879494597874	0.0
3	0.0	0.0	0.0	1.7522790545335445	0.0
4	0.0	0.0	0.0	2.4468080639269023	0.0
5	0.0	0.0	0.0	4.446833258449613	0.0
6	0.0	0.0	0.0	5.833581779321175	0.0
7	0.0	0.0	0.0	6.743314003535631	0.0
8	0.0	0.0	0.0	8.559209227913852	0.0
9	0.0	0.0	0.0	9.400076423385556	0.0
10	0.0	0.0	0.0	10.72089927649729	0.0
11	0.0	0.0	0.0	13.205499124490336	0.0
12	0.0	0.0	0.0	14.776797524218235	0.0
13	0.0	0.0	0.0	15.445922056544907	0.0
14	0.0	0.0	0.0	15.80599377695289	0.0
15	0.0	0.0	0.0	16.303795554846378	0.0
16	0.0	0.0	0.0	17.1347948955897	0.0
17	0.0	0.0	0.0	18.035289128143543	0.0
18	0.0	0.0	0.0	19.12033323955372	0.0
19	0.0	0.0	0.0	19.713874203748105	0.0
20	0.0	0.0	0.0	20.55201199259281	0.0
21	0.0	0.0	0.0	21.252839632663857	0.0
22	0.0	0.0	0.0	21.938130650396605	0.0
23	2.0995435592302233E-4	0.0	0.0	22.533141295082448	0.0
24	2.0995435592302233E-4	0.0	0.0	22.997560330384175	0.0
25	2.0995435592302233E-4	0.0	0.0	23.550160195173568	0.0
26	2.0995435592302233E-4	0.0	0.0	24.031375578949138	0.0
27	2.0995435592302233E-4	0.0	0.0	24.837180396981697	0.0
28	4.1990871184604465E-4	0.0	0.0	25.375713319924248	0.0
29	4.1990871184604465E-4	0.0	0.0	26.085149088588143	0.0
30	4.1990871184604465E-4	0.0	0.0	27.545171679676837	0.0
31	4.1990871184604465E-4	0.0	0.0	28.108479216618306	0.0
32	4.1990871184604465E-4	0.0	0.0	28.53237706122689	0.0
33	4.1990871184604465E-4	0.0	0.0	29.0303887934763	0.0
34	4.1990871184604465E-4	0.0	0.0	29.623299894602912	0.0
35	4.1990871184604465E-4	0.0	0.0	30.142307062444626	0.0
36	4.1990871184604465E-4	0.0	0.0	30.50678782432699	0.0
37	4.1990871184604465E-4	0.0	0.0	31.019706315846935	0.0
38	4.1990871184604465E-4	0.0	0.0	31.570836500144868	0.0
39	4.1990871184604465E-4	0.0	0.0	33.2500514388172	0.0
40	4.1990871184604465E-4	0.0	0.0	33.70439266503462	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	20	6.3077116E-4	46.000004	31
ACTGATC	20	6.3077116E-4	46.000004	16
ATGTGCG	20	6.3077116E-4	46.000004	1
GTAGGGT	40	5.5988494E-9	46.000004	4
TACGGGA	40	5.5988494E-9	46.000004	4
AGTGCGA	20	6.3077116E-4	46.000004	29
CTCATGC	20	6.3077116E-4	46.000004	23
TCGAGTC	20	6.3077116E-4	46.000004	16
CGGTATG	20	6.3077116E-4	46.000004	31
CACGTAA	20	6.3077116E-4	46.000004	33
TAGTGCG	20	6.3077116E-4	46.000004	1
TCGGTAT	20	6.3077116E-4	46.000004	30
GTTGCGC	20	6.3077116E-4	46.000004	41
TACCGGG	20	6.3077116E-4	46.000004	3
TTTAGCG	30	1.8590908E-6	46.0	1
GCGACTC	30	1.8590908E-6	46.0	43
CTCGTTT	25	3.4141216E-5	46.0	22
CTCGTAA	60	0.0	46.0	33
GGTCGCT	25	3.4141216E-5	46.0	10
CGAAAGG	50	1.6370905E-11	46.0	2
>>END_MODULE