FastQCFastQC Report
Sat 14 Jan 2017
SRR2933035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375454
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139453.714170044799096No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC68781.831915494308224No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG25690.6842382821863664No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG22810.6075311489556643No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT21400.5699766149781331Illumina Single End Adapter 2 (95% over 22bp)
GGGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT19570.5212356240711246No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC16550.4407996718639301No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT16120.4293468707218461Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT14660.3904606156812819Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10900.2903151917411987No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTCCCATGGTCG10700.28498830748906656No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC10650.2836565864260336No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT9110.24263957768461653No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC7400.19709471732888717No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA7260.1933658983523947No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGT7050.187772669887656No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT6710.17871696665903147No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTCCCATGGT5940.15820846228832294No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG5770.15368061067401068No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC5610.14941910327230498No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG5320.14169512110671348No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC4670.12438274728728418No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT4450.1185231746099389Illumina Single End Adapter 2 (95% over 21bp)
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG4410.11745779775951248No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG4230.11266360193259362No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG4130.11000015980652757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAG351.0171061E-746.0000041
TCACGAG206.3059997E-446.01
ACAACGC206.3059997E-446.013
ACAACGA253.4127304E-546.013
CTAGATA253.4127304E-546.019
GTTTGCG206.3059997E-446.01
ACACGCG253.4127304E-546.036
ACCGCTG206.3059997E-446.041
TTATGCG206.3059997E-446.01
CACGCGG253.4127304E-546.037
ACCGACT206.3059997E-446.039
TCGGGCA206.3059997E-446.05
ACTTCAG206.3059997E-446.01
ATTAACG206.3059997E-446.01
CGACAGG253.4127304E-546.02
GTAATAG206.3059997E-446.01
GCCGAGT253.4127304E-546.031
TACGAAG206.3059997E-446.01
AACGAGC253.4127304E-546.015
CGAATAG206.3059997E-446.026
Produced by FastQC (version 0.11.5)