FastQCFastQC Report
Sat 14 Jan 2017
SRR2933034.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933034.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333929
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286972.1513139005149453No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC255761.917343426824066No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG154131.1554587987816443No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA34330.2573600244090952No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT32940.24693967969809488TruSeq Adapter, Index 21 (95% over 24bp)
GGGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT31600.23689416753065567No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT29350.2200267030704033TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT24030.18014452043549545TruSeq Adapter, Index 15 (95% over 22bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG23260.17437209926465352No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC21470.16095309420516385TruSeq Adapter, Index 21 (95% over 22bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTATAGGACTCG17920.1343399836123212No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG17850.13381521805133556No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC17460.13089152421155847No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTATAGGACT16430.1231699738141985No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT16310.12227037570965171No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA15520.11634802152138532No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT15110.11327439466418376No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC13520.10135471977893876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG405.6134013E-946.0000041
AATCCCG206.31182E-446.00000439
ATTAGCG750.046.0000041
GTCGAAC206.31182E-446.00000440
CTATCCG206.31182E-446.00000429
CGGCAAT206.31182E-446.00000435
CCGGATC206.31182E-446.00000437
ATCGTAG405.6134013E-946.0000041
CGTTACC206.31182E-446.00000426
CCCGTAG206.31182E-446.00000441
ACGGCAC405.6134013E-946.00000433
CAGATCG206.31182E-446.00000419
ACTACCG351.01974365E-746.0000041
ACTCGAC351.01974365E-746.00000426
TATAGTC206.31182E-446.00000421
TTACGGC206.31182E-446.00000418
TTACGAT351.01974365E-746.00000417
ACGTATG206.31182E-446.0000041
CCGCGTA206.31182E-446.00000428
ATAACCG206.31182E-446.00000425
Produced by FastQC (version 0.11.5)