##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933034.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1333929
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44777645586834	33.0	31.0	34.0	31.0	34.0
2	32.81752552047373	34.0	31.0	34.0	31.0	34.0
3	32.842014080209665	34.0	31.0	34.0	31.0	34.0
4	36.312829243535454	37.0	37.0	37.0	35.0	37.0
5	36.15843122085209	37.0	35.0	37.0	35.0	37.0
6	36.2512180183503	37.0	37.0	37.0	35.0	37.0
7	36.50670238071142	37.0	37.0	37.0	35.0	37.0
8	36.46200285022666	37.0	37.0	37.0	35.0	37.0
9	38.293065822843644	39.0	39.0	39.0	37.0	39.0
10	38.00344920906585	39.0	38.0	39.0	35.0	39.0
11	37.6708153132588	39.0	37.0	39.0	35.0	39.0
12	37.206691660500674	39.0	35.0	39.0	35.0	39.0
13	37.07390423328378	39.0	35.0	39.0	34.0	39.0
14	37.65653944100473	40.0	36.0	41.0	31.0	41.0
15	38.03512855631747	40.0	36.0	41.0	33.0	41.0
16	38.35242505410708	40.0	36.0	41.0	33.0	41.0
17	38.4404259896891	40.0	36.0	41.0	35.0	41.0
18	38.395970100357665	40.0	36.0	41.0	35.0	41.0
19	38.335172261792046	40.0	36.0	41.0	35.0	41.0
20	38.14479931090785	40.0	35.0	41.0	34.0	41.0
21	38.090612768745565	40.0	35.0	41.0	34.0	41.0
22	38.06528383444696	40.0	35.0	41.0	34.0	41.0
23	38.04724614278571	40.0	35.0	41.0	34.0	41.0
24	37.98709301619501	40.0	35.0	41.0	34.0	41.0
25	37.81058287210189	40.0	35.0	41.0	34.0	41.0
26	37.721019634478296	40.0	35.0	41.0	34.0	41.0
27	37.62251139303516	39.0	35.0	41.0	34.0	41.0
28	37.58831092209555	39.0	35.0	41.0	34.0	41.0
29	37.52467934950061	39.0	35.0	41.0	34.0	41.0
30	37.49003432716434	39.0	35.0	41.0	34.0	41.0
31	37.35589525379537	40.0	35.0	41.0	34.0	41.0
32	37.255856945909414	39.0	35.0	41.0	33.0	41.0
33	37.16030238490954	39.0	35.0	41.0	33.0	41.0
34	36.98147202737177	39.0	35.0	41.0	33.0	41.0
35	36.89181058362177	39.0	35.0	41.0	33.0	41.0
36	36.83080058983649	39.0	35.0	41.0	33.0	41.0
37	36.682304680384036	39.0	35.0	41.0	32.0	41.0
38	36.67350136326596	39.0	35.0	41.0	32.0	41.0
39	36.64696996616762	39.0	35.0	41.0	33.0	41.0
40	36.55929888322392	39.0	35.0	41.0	32.0	41.0
41	36.392795268713705	38.0	35.0	41.0	32.0	41.0
42	36.448286228127586	38.0	35.0	41.0	32.0	41.0
43	36.4258727413528	38.0	35.0	41.0	32.0	41.0
44	36.41502508754214	38.0	35.0	41.0	32.0	41.0
45	36.369820282788666	38.0	35.0	41.0	32.0	41.0
46	36.34059159070685	38.0	35.0	41.0	32.0	41.0
47	36.24741496736333	38.0	35.0	41.0	32.0	41.0
48	36.11900408492506	37.0	35.0	41.0	31.0	41.0
49	36.097654372908906	37.0	35.0	41.0	31.0	41.0
50	35.978384156877915	37.0	35.0	40.0	31.0	41.0
51	35.913438421385244	37.0	35.0	40.0	31.0	41.0
52	35.70031463443706	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	3.0
14	9.0
15	41.0
16	66.0
17	132.0
18	296.0
19	487.0
20	857.0
21	1355.0
22	2175.0
23	3324.0
24	5278.0
25	8317.0
26	10477.0
27	11968.0
28	13253.0
29	14955.0
30	17481.0
31	21859.0
32	28445.0
33	41023.0
34	112849.0
35	156405.0
36	104973.0
37	105586.0
38	149978.0
39	518402.0
40	3931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.9060654652534	22.822953845369582	26.045913987925896	13.225066701451127
2	38.177894025843955	23.01329381098994	26.21376400093258	12.595048162233521
3	32.44543000414565	22.770027490218745	31.153757059033875	13.630785446601731
4	28.942469951549143	26.736280566656845	30.58116286548984	13.740086616304167
5	24.297395138721775	30.70358317421692	30.423883130211575	14.575138556849726
6	25.269335924175873	37.96446437554023	27.315396846458846	9.450802853825053
7	85.15528187782108	1.6339700238918262	10.14701682023556	3.0637312780515304
8	82.86423040506654	1.9090971108657206	10.601688695575252	4.624983788492491
9	77.34587073225036	3.367495571353498	13.754255286450778	5.532378409945357
10	51.66279464649168	17.595539192865587	18.50173435017906	12.239931810463675
11	33.42839086638044	23.67232438907918	27.368698034153244	15.530586710387134
12	31.506624415542355	17.777782775545028	32.405322921984606	18.310269886928015
13	29.1867108369336	19.810649592294645	35.561937704330596	15.440701866441167
14	17.091239488758397	22.83644781693778	40.60351038173696	19.46880231256686
15	13.065088171859221	30.223122819880217	39.88375693159082	16.828032076669746
16	26.79093115150806	26.08789523280474	31.543657870846197	15.577515744840992
17	24.344024307140785	25.34617659560591	27.24537812732162	23.064420969931685
18	21.46403594194294	26.084821605947546	34.45160874379371	17.99953370831581
19	21.732565976150156	28.26739654059549	27.59652125412972	22.40351622912464
20	16.185044331444928	40.19164438287195	24.593362915117673	19.02994837056545
21	20.968357386337654	29.82669992180993	33.27665865274689	15.928284039105531
22	17.935887142419123	24.36306580035369	36.937123340147785	20.763923717079393
23	18.477070368812733	26.220510986716683	33.969349193247915	21.333069451222666
24	21.008014669446425	31.049928444467433	29.226817919094643	18.715238966991496
25	23.472313743834942	26.06345615096456	30.45724322658852	20.00698687861198
26	16.652310580248276	31.399122442049016	26.491439949202693	25.457127028500015
27	22.128464108659458	27.456333882837843	32.03671259864655	18.378489409856147
28	16.916267657424044	31.1342657667687	26.188050488444286	25.76141608736297
29	20.02475394117678	31.540734177006424	27.071080994565676	21.36343088725112
30	25.001855421090628	26.699472010879138	26.557410476869457	21.741262091160774
31	21.791114819454407	27.3629256129824	31.351143876473188	19.494815691090007
32	26.00925536516561	27.419300427533997	25.740425464923543	20.831018742376845
33	25.50690479028494	28.375123413614965	28.19715292193213	17.920818874167963
34	19.4678277479536	26.713715647534464	34.67785766708723	19.140598937424706
35	21.224592913116066	34.526650219014655	26.401705038274155	17.847051829595127
36	27.166138527612787	29.29653677219702	23.586637669621098	19.950687030569092
37	20.25842454883281	33.13557168335046	26.213913933950007	20.39208983386672
38	23.301540036988474	29.17906425304495	28.25480216713183	19.26459354283474
39	20.505064362496057	30.291342342808353	24.277079214860763	24.92651407983483
40	27.728312376445825	25.544238111623628	27.965356476993904	18.76209303493664
41	19.29045698834046	27.553490478128893	25.264313168092155	27.89173936543849
42	17.879512327867523	28.78009249367845	31.261259032527217	22.079136145926807
43	18.71906225893582	28.75497871325985	31.434356701143763	21.091602326660563
44	23.687692523365186	27.319070205385742	27.78798571738076	21.205251553868308
45	19.10184125242048	27.34920674188806	25.083269049552115	28.465682956139343
46	22.216249890361482	25.433887410799226	28.289961459717873	24.059901239121423
47	18.30104900635641	26.298850988320964	31.599957718889087	23.800142286433537
48	18.585621873428046	31.682870677524814	27.605817101210032	22.125690347837104
49	25.00432931587813	25.92994079894807	28.237634836636733	20.828095048537065
50	20.59547397200301	27.28600997504365	27.9757768217049	24.142739231248438
51	18.932716808765683	27.64937264277184	26.68762730250261	26.730283245959868
52	19.15146908118798	28.94569351142377	28.41073250525328	23.492104902134972
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1624.0
1	2785.5
2	3947.0
3	17974.5
4	32002.0
5	17748.0
6	3494.0
7	4673.5
8	5853.0
9	8872.5
10	11892.0
11	14934.5
12	17977.0
13	18886.0
14	18077.5
15	16360.0
16	14371.5
17	12383.0
18	10896.0
19	9409.0
20	8194.5
21	6980.0
22	6403.5
23	5827.0
24	6172.5
25	6518.0
26	7168.5
27	7819.0
28	8923.5
29	10028.0
30	11651.0
31	13274.0
32	14041.0
33	14808.0
34	17155.0
35	19502.0
36	20787.0
37	22072.0
38	24596.0
39	32027.5
40	36935.0
41	46707.5
42	56480.0
43	69964.5
44	83449.0
45	104231.0
46	125013.0
47	128791.0
48	132569.0
49	135340.0
50	138111.0
51	114289.5
52	90468.0
53	82330.5
54	74193.0
55	70567.5
56	66942.0
57	65458.0
58	63974.0
59	71111.0
60	78248.0
61	58663.0
62	39078.0
63	31801.5
64	21480.0
65	18435.0
66	15549.5
67	12664.0
68	10697.0
69	8730.0
70	7453.0
71	6176.0
72	5158.0
73	4140.0
74	3277.0
75	2414.0
76	2005.5
77	1597.0
78	1178.0
79	759.0
80	506.0
81	253.0
82	142.5
83	32.0
84	31.5
85	31.0
86	22.0
87	13.0
88	11.0
89	6.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1333929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.428624502757568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.53713636289088	14.472279351119225
2	12.283424468033585	5.264337810669526
3	5.479047814701447	3.522253747616752
4	3.130837034298401	2.683581247492302
5	1.969237040191511	2.10990205455928
6	1.3229348303746638	1.7009204233030766
7	0.9302210855394452	1.3953350842600607
8	0.7414613027948448	1.2710796672732927
9	0.5391114141743513	1.039717445354351
>10	4.333444601779927	19.526614985555593
>50	0.9923762047858788	15.384629131051536
>100	0.7242516694197483	22.169324714021503
>500	0.00843378945466433	1.3235058088947418
>1k	0.007028157878886941	2.8890318538948376
>5k	0.0	0.0
>10k+	0.0010542236818330412	5.247486674933943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28697	2.1513139005149453	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	25576	1.917343426824066	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	15413	1.1554587987816443	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	3433	0.2573600244090952	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT	3294	0.24693967969809488	TruSeq Adapter, Index 21 (95% over 24bp)
GGGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	3160	0.23689416753065567	No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2935	0.2200267030704033	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2403	0.18014452043549545	TruSeq Adapter, Index 15 (95% over 22bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2326	0.17437209926465352	No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	2147	0.16095309420516385	TruSeq Adapter, Index 21 (95% over 22bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTATAGGACTCG	1792	0.1343399836123212	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG	1785	0.13381521805133556	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	1746	0.13089152421155847	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTATAGGACT	1643	0.1231699738141985	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1631	0.12227037570965171	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1552	0.11634802152138532	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1511	0.11327439466418376	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1352	0.10135471977893876	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25286203388636125	0.0
2	0.0	0.0	0.0	0.778302293450401	0.0
3	0.0	0.0	0.0	1.2405457861700286	0.0
4	0.0	0.0	0.0	1.8117905825572425	0.0
5	0.0	0.0	0.0	3.512180933168107	0.0
6	0.0	0.0	0.0	5.397438694263338	0.0
7	0.0	0.0	0.0	7.062070020218467	0.0
8	0.0	0.0	0.0	9.931038308635618	0.0
9	0.0	0.0	0.0	11.615535759399489	0.0
10	0.0	0.0	0.0	13.172964977896125	0.0
11	0.0	0.0	0.0	15.271502456277657	0.0
12	0.0	0.0	0.0	16.429959915407792	0.0
13	0.0	0.0	0.0	16.942730834999463	0.0
14	0.0	0.0	0.0	17.386532566575884	0.0
15	0.0	0.0	0.0	17.722682391641534	0.0
16	0.0	0.0	0.0	18.238826804125257	0.0
17	0.0	0.0	0.0	18.83788417524471	0.0
18	0.0	0.0	0.0	19.513857184302914	0.0
19	7.496650871223281E-5	0.0	0.0	19.91995076199708	0.0
20	7.496650871223281E-5	0.0	0.0	20.4673562086138	0.0
21	7.496650871223281E-5	0.0	0.0	20.973754974964933	0.0
22	7.496650871223281E-5	0.0	0.0	21.52220995270363	0.0
23	1.4993301742446561E-4	0.0	0.0	22.00874259424602	0.0
24	2.2489952613669842E-4	0.0	0.0	22.356437261653355	0.0
25	2.2489952613669842E-4	0.0	0.0	22.735243030176267	0.0
26	2.2489952613669842E-4	0.0	0.0	23.054675323799092	0.0
27	2.2489952613669842E-4	0.0	0.0	23.527489094247144	0.0
28	2.2489952613669842E-4	0.0	0.0	23.881331015368882	0.0
29	2.2489952613669842E-4	0.0	0.0	24.365239829106347	0.0
30	2.2489952613669842E-4	0.0	0.0	25.893956874766197	0.0
31	2.2489952613669842E-4	0.0	0.0	26.448858972254147	0.0
32	2.2489952613669842E-4	0.0	0.0	26.798727668414134	0.0
33	2.2489952613669842E-4	0.0	0.0	27.194925666958287	0.0
34	2.2489952613669842E-4	0.0	0.0	27.65536996346882	0.0
35	2.2489952613669842E-4	0.0	0.0	28.109442106738815	0.0
36	2.2489952613669842E-4	0.0	0.0	28.423101979190797	0.0
37	2.2489952613669842E-4	0.0	0.0	28.827246427658444	0.0
38	2.2489952613669842E-4	0.0	0.0	29.32270008373759	0.0
39	2.2489952613669842E-4	0.0	0.0	31.413890844265325	0.0
40	2.2489952613669842E-4	0.0	0.0	31.872610911075476	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	40	5.6134013E-9	46.000004	1
AATCCCG	20	6.31182E-4	46.000004	39
ATTAGCG	75	0.0	46.000004	1
GTCGAAC	20	6.31182E-4	46.000004	40
CTATCCG	20	6.31182E-4	46.000004	29
CGGCAAT	20	6.31182E-4	46.000004	35
CCGGATC	20	6.31182E-4	46.000004	37
ATCGTAG	40	5.6134013E-9	46.000004	1
CGTTACC	20	6.31182E-4	46.000004	26
CCCGTAG	20	6.31182E-4	46.000004	41
ACGGCAC	40	5.6134013E-9	46.000004	33
CAGATCG	20	6.31182E-4	46.000004	19
ACTACCG	35	1.01974365E-7	46.000004	1
ACTCGAC	35	1.01974365E-7	46.000004	26
TATAGTC	20	6.31182E-4	46.000004	21
TTACGGC	20	6.31182E-4	46.000004	18
TTACGAT	35	1.01974365E-7	46.000004	17
ACGTATG	20	6.31182E-4	46.000004	1
CCGCGTA	20	6.31182E-4	46.000004	28
ATAACCG	20	6.31182E-4	46.000004	25
>>END_MODULE