##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933029.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2708534
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53529843081165	33.0	31.0	34.0	31.0	34.0
2	32.89264709248619	34.0	31.0	34.0	31.0	34.0
3	32.9360864585787	34.0	31.0	34.0	31.0	34.0
4	36.385604168158864	37.0	37.0	37.0	35.0	37.0
5	36.367575596245054	37.0	37.0	37.0	35.0	37.0
6	36.49885731543337	37.0	37.0	37.0	35.0	37.0
7	36.54646720329152	37.0	37.0	37.0	35.0	37.0
8	36.397263981179485	37.0	37.0	37.0	35.0	37.0
9	38.066036830255776	39.0	39.0	39.0	35.0	39.0
10	37.97353217644674	39.0	38.0	39.0	35.0	39.0
11	37.69285967981203	39.0	37.0	39.0	35.0	39.0
12	37.589685416538984	39.0	37.0	39.0	35.0	39.0
13	37.54508675172621	39.0	37.0	39.0	35.0	39.0
14	39.01521930313594	41.0	38.0	41.0	35.0	41.0
15	39.071135529404465	41.0	38.0	41.0	35.0	41.0
16	39.0533018968933	41.0	38.0	41.0	35.0	41.0
17	39.04671678479945	41.0	38.0	41.0	35.0	41.0
18	38.92423945942713	40.0	38.0	41.0	35.0	41.0
19	38.78229477643626	40.0	37.0	41.0	35.0	41.0
20	38.63393407651519	40.0	37.0	41.0	35.0	41.0
21	38.570872287370214	40.0	36.0	41.0	35.0	41.0
22	38.52340380441966	40.0	36.0	41.0	35.0	41.0
23	38.47197524564949	40.0	36.0	41.0	35.0	41.0
24	38.39641665934413	40.0	36.0	41.0	35.0	41.0
25	38.32121730796069	40.0	35.0	41.0	35.0	41.0
26	38.23259667406796	40.0	35.0	41.0	35.0	41.0
27	38.1491212589541	40.0	35.0	41.0	35.0	41.0
28	38.110846310218	40.0	35.0	41.0	35.0	41.0
29	38.03862679958974	40.0	35.0	41.0	34.0	41.0
30	37.95893055062259	40.0	35.0	41.0	34.0	41.0
31	37.80242522338653	40.0	35.0	41.0	34.0	41.0
32	37.666941600142366	40.0	35.0	41.0	34.0	41.0
33	37.418797401103326	40.0	35.0	41.0	33.0	41.0
34	37.21688854561176	40.0	35.0	41.0	33.0	41.0
35	37.064468084949276	40.0	35.0	41.0	33.0	41.0
36	36.99158363897222	40.0	35.0	41.0	33.0	41.0
37	36.893724058845116	40.0	35.0	41.0	33.0	41.0
38	36.87244280485311	40.0	35.0	41.0	33.0	41.0
39	36.79934015965832	40.0	35.0	41.0	32.0	41.0
40	36.7599254799829	39.0	35.0	41.0	32.0	41.0
41	36.6896402260411	39.0	35.0	41.0	32.0	41.0
42	36.60220325829397	39.0	35.0	41.0	32.0	41.0
43	36.4939162661425	39.0	35.0	41.0	32.0	41.0
44	36.400689081252075	39.0	35.0	41.0	31.0	41.0
45	36.318076125313546	39.0	35.0	41.0	31.0	41.0
46	36.22197432264095	38.0	35.0	41.0	31.0	41.0
47	36.11060115915104	38.0	35.0	41.0	31.0	41.0
48	36.0097709683541	38.0	35.0	41.0	31.0	41.0
49	35.915730428342414	38.0	35.0	41.0	31.0	41.0
50	35.816013016635566	38.0	35.0	41.0	31.0	41.0
51	35.71032817014665	37.0	35.0	40.0	30.0	41.0
52	35.46482192950135	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	8.0
13	14.0
14	21.0
15	40.0
16	91.0
17	190.0
18	404.0
19	806.0
20	1441.0
21	2377.0
22	3868.0
23	6564.0
24	11555.0
25	19202.0
26	26777.0
27	29767.0
28	30124.0
29	30117.0
30	32999.0
31	39069.0
32	48425.0
33	63880.0
34	139117.0
35	264332.0
36	202838.0
37	232197.0
38	361707.0
39	1148711.0
40	11888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.884259160121303	21.599876538378325	24.154838004617996	22.361026296882372
2	31.41599847002105	22.394033082102716	24.875486148595513	21.31448229928072
3	26.519475110890244	23.74804968296503	28.946544514486433	20.78593069165829
4	23.742253189363694	27.17174678257685	26.514933908896843	22.571066119162616
5	21.83361921984365	36.448093322808575	23.944281297558014	17.77400615978976
6	84.48714322951088	2.819311110733703	10.179381170773562	2.5141644889818626
7	85.72434387015264	2.370618201580634	8.625699363567154	3.2793385646995756
8	78.7263885186599	4.736141396046718	12.26419900950108	4.273271075792292
9	38.27217971050022	29.744466933034623	16.97490229031646	15.008451066148698
10	29.9972974310088	22.979626617203255	28.58110697521242	18.44196897657552
11	27.843512394527814	20.446743515126634	31.97995668505546	19.729787405290093
12	21.755458857079145	24.976426362009853	33.618112233407444	19.650002547503558
13	18.493066729086657	28.950605752041508	33.060282795047065	19.49604472382477
14	17.13495197032786	26.91315671134274	36.76686355054063	19.185027767788775
15	19.872927568935815	22.37874806075907	37.37054805293196	20.37777631737316
16	19.209912077898966	24.601241852603657	31.140535802762674	25.048310266734696
17	20.54336404859603	26.655821931716567	31.585352076067718	21.215461943619683
18	23.45600978241366	29.691449322770175	27.58145181120119	19.271089083614974
19	23.513790116719967	26.433007671308538	27.39083208850249	22.662370123469007
20	22.880384739493763	28.25945696085041	28.701799571280993	20.158358728374832
21	20.267938301679063	25.12285243604105	29.17471222439888	25.43449703788101
22	18.693027298162033	29.788180617263805	27.698193930738917	23.82059815383525
23	18.15723930362329	29.057637821788468	32.023522687919	20.761600186669245
24	22.785277940022166	26.855265616012204	29.308326939960878	21.051129504004752
25	19.18709530690772	29.28635933682206	27.51396142710411	24.01258392916611
26	18.487011793095455	27.99676873171982	30.27276009826718	23.24345937691755
27	21.026946680381343	26.885724897675274	31.618063498556786	20.4692649233866
28	20.9124197813282	27.649200637688136	28.662331726313937	22.77604785466972
29	22.66163171664081	26.678564862024995	29.38224146346326	21.277561957870937
30	26.568874527696533	26.579913709778058	26.092860565900228	20.758351196625185
31	25.405403808850103	27.760220104307347	26.75986345380933	20.07451263303322
32	24.785326674872827	27.574067742919233	25.430066596911832	22.210538985296104
33	21.57048794661614	26.740775637300473	29.349086996877276	22.339649419206108
34	20.52977736295723	27.65477560924101	29.60970768688892	22.205739340912835
35	24.507390344739996	28.379780353504884	27.394450281960648	19.718379019794472
36	20.56581161617318	30.313224792452303	28.125805324947002	20.99515826642752
37	20.760197213695676	29.906288789433695	29.105043540158626	20.228470456712007
38	19.42187175793252	28.290580808658856	27.141915146717743	25.145632286690883
39	22.170554255549312	27.089303660208806	29.560382110765453	21.17975997347643
40	19.031549908548314	26.44393609236583	31.4090205254946	23.115493473591247
41	21.038945791339522	29.58596790736243	26.276243901682605	23.09884239961544
42	20.758609639015056	27.14280123491158	28.12776210304172	23.970827023031646
43	21.828893416143195	27.52429912269885	30.22096085926926	20.425846601888697
44	21.528066474336303	28.771283653814205	27.392899627621436	22.307750244228057
45	22.47477786876591	27.558487358844303	27.78739347558495	22.17934129680484
46	23.775370735608266	27.18333976977952	27.422546661773488	21.618742832838723
47	21.178615442892724	28.799416953968453	28.23542181859264	21.78654578454618
48	20.638803131140314	27.895717757281247	27.819144969197357	23.646334142381082
49	20.223744653011554	26.82440021059363	29.948193376933794	23.00366175946102
50	19.577601757998977	27.0805904596361	30.716948725768255	22.62485905659667
51	20.03356797440977	24.988130110236757	29.880629152153897	25.097672763199576
52	20.07466031439886	24.84930962653598	30.065489301592667	25.010540757472494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4573.0
1	4542.0
2	4511.0
3	32702.0
4	60893.0
5	36104.0
6	11315.0
7	12870.0
8	14425.0
9	17397.0
10	20369.0
11	23382.5
12	26396.0
13	26562.0
14	25151.5
15	23575.0
16	20652.5
17	17730.0
18	16663.5
19	15597.0
20	15063.5
21	14530.0
22	14685.5
23	14841.0
24	15348.5
25	15856.0
26	16947.0
27	18038.0
28	19229.5
29	20421.0
30	22425.0
31	24429.0
32	28739.0
33	33049.0
34	38873.5
35	44698.0
36	51161.0
37	57624.0
38	63231.0
39	74984.0
40	81130.0
41	97007.0
42	112884.0
43	143304.5
44	173725.0
45	204533.0
46	235341.0
47	242019.5
48	248698.0
49	251966.0
50	255234.0
51	225698.5
52	196163.0
53	174611.5
54	153060.0
55	145807.0
56	138554.0
57	132112.5
58	125671.0
59	119463.5
60	113256.0
61	106716.0
62	100176.0
63	89248.0
64	66248.5
65	54177.0
66	44137.5
67	34098.0
68	28883.5
69	23669.0
70	19854.0
71	16039.0
72	14510.5
73	12982.0
74	10109.0
75	7236.0
76	6044.5
77	4853.0
78	3805.5
79	2758.0
80	1955.5
81	1153.0
82	816.0
83	479.0
84	362.0
85	245.0
86	161.0
87	77.0
88	67.5
89	52.0
90	46.0
91	28.5
92	11.0
93	7.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2708534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.513577694728788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.25699955148124	16.492771510849852
2	10.593646224253588	4.770017546804069
3	4.167127220592778	2.8145082733390416
4	2.245603665836206	2.0222629040948483
5	1.3499730251615816	1.5196361293881662
6	0.9344184102435926	1.262226088704245
7	0.6809679060140472	1.0731716701764817
8	0.5172841940920568	0.931673431515735
9	0.4081056206600611	0.826912583854722
>10	3.9233547214172453	20.238236766037087
>50	1.2197423151373183	19.725105052670322
>100	0.6923499423284077	21.86104569660727
>500	0.004799823502745508	0.7306427575364508
>1k	0.004799823502745508	2.450194539055963
>5k	4.965334658012595E-4	0.8869961342512075
>10k+	3.3102231053417296E-4	2.394598915114491
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49532	1.8287383507092767	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14731	0.5438735493074852	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9246	0.3413654766748359	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9161	0.3382272476550045	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	5397	0.19925908258858852	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	4825	0.17814064730219373	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	4756	0.17559314374491883	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4713	0.17400556906429826	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	4522	0.16695378385503007	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4102	0.15144724046292202	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4048	0.1494535420267938	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	3654	0.1349069275113401	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT	3482	0.12855662878885774	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13453772409724227	0.0
2	0.0	0.0	0.0	0.4229963515318619	0.0
3	0.0	0.0	0.0	0.9031823119074747	0.0
4	0.0	0.0	0.0	1.252079538229906	0.0
5	0.0	0.0	0.0	2.9721613241701967	0.0
6	0.0	0.0	0.0	3.781344446848369	0.0
7	0.0	0.0	0.0	5.315606154473231	0.0
8	0.0	0.0	0.0	7.0104344268892325	0.0
9	0.0	0.0	0.0	8.514864498654992	0.0
10	0.0	0.0	0.0	9.43248266405369	0.0
11	0.0	0.0	0.0	10.262784222018258	0.0
12	0.0	0.0	0.0	10.847565509607781	0.0
13	0.0	0.0	0.0	11.143002081568849	0.0
14	0.0	0.0	0.0	11.33151734480719	0.0
15	0.0	0.0	0.0	11.763965303739957	0.0
16	0.0	0.0	0.0	12.31655205362015	0.0
17	0.0	0.0	0.0	12.968639123599704	0.0
18	0.0	0.0	0.0	13.32196679089131	0.0
19	0.0	0.0	0.0	13.69951420214773	0.0
20	3.692034140978109E-5	0.0	0.0	14.122325951972543	0.0
21	7.384068281956217E-5	0.0	0.0	14.563376350453789	0.0
22	1.1076102422934326E-4	0.0	0.0	15.049321884089327	0.0
23	1.1076102422934326E-4	0.0	0.0	15.417491528627664	0.0
24	1.4768136563912435E-4	0.0	0.0	15.710565198738506	0.0
25	1.4768136563912435E-4	0.0	0.0	15.99311657154756	0.0
26	1.4768136563912435E-4	0.0	0.0	16.291986735259737	0.0
27	1.8460170704890543E-4	0.0	0.0	16.62412212658213	0.0
28	1.8460170704890543E-4	0.0	0.0	16.973720839391348	0.0
29	1.8460170704890543E-4	0.0	0.0	17.32387335732171	0.0
30	0.0027321052643238	0.0	0.0	17.64434192075861	0.0
31	0.0027321052643238	0.0	0.0	17.944873499834227	0.0
32	0.0027321052643238	0.0	0.0	18.26508362088126	0.0
33	0.0027321052643238	0.0	0.0	18.59880658688427	0.0
34	0.0027321052643238	0.0	0.0	18.994186523041616	0.0
35	0.0027690256057335814	0.0	0.0	19.304797355322105	0.0
36	0.0027690256057335814	0.0	0.0	19.648488813505757	0.0
37	0.0027690256057335814	0.0	0.0	20.013077184927344	0.0
38	0.0027690256057335814	0.0	0.0	20.551375762681953	0.0
39	0.0027690256057335814	0.0	0.0	20.926966395843657	0.0
40	0.0027690256057335814	0.0	0.0	21.273500720315862	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAA	30	1.862345E-6	46.0	28
ATATACG	20	6.3129776E-4	46.0	29
CCGCGAA	20	6.3129776E-4	46.0	46
CGTTTTT	14810	0.0	44.151924	1
CGACGGT	695	0.0	43.683456	27
CGGTCTA	705	0.0	43.063828	30
TAATCGG	55	4.7293724E-11	41.81818	1
TCACGAC	760	0.0	41.460526	24
AATGCGG	350	0.0	41.4	1
CCGCACT	5940	0.0	41.314816	33
CGCACTT	5955	0.0	41.210747	34
CCCGCAC	6060	0.0	41.179867	32
CCAATCG	190	0.0	41.157894	23
TACGGGA	1080	0.0	41.101852	3
ACCCGCA	6045	0.0	41.09181	31
TAACGGG	845	0.0	40.8284	2
CAACGCA	6410	0.0	40.76131	16
TATCCGG	170	0.0	40.588234	1
TATGACC	6265	0.0	40.529926	27
TCAACGC	6540	0.0	40.408257	15
>>END_MODULE