##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933028.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1863454
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.570283999497704	33.0	31.0	34.0	31.0	34.0
2	32.94550657005754	34.0	31.0	34.0	31.0	34.0
3	33.03234048170763	34.0	33.0	34.0	31.0	34.0
4	36.446714005282665	37.0	37.0	37.0	35.0	37.0
5	36.42752383477134	37.0	37.0	37.0	35.0	37.0
6	36.533164757487974	37.0	37.0	37.0	35.0	37.0
7	36.56617281671563	37.0	37.0	37.0	35.0	37.0
8	36.43708564847858	37.0	37.0	37.0	35.0	37.0
9	38.12349808473942	39.0	39.0	39.0	37.0	39.0
10	38.026178268956464	39.0	39.0	39.0	35.0	39.0
11	37.68242790001792	39.0	37.0	39.0	35.0	39.0
12	37.570986458479794	39.0	37.0	39.0	35.0	39.0
13	37.49130324655183	39.0	37.0	39.0	35.0	39.0
14	38.981041657051904	41.0	38.0	41.0	35.0	41.0
15	39.034333554785896	41.0	38.0	41.0	35.0	41.0
16	39.028003374378976	41.0	38.0	41.0	35.0	41.0
17	39.025948587944754	41.0	38.0	41.0	35.0	41.0
18	38.94426479000823	41.0	38.0	41.0	35.0	41.0
19	38.81870816236945	41.0	37.0	41.0	35.0	41.0
20	38.70486365641438	41.0	37.0	41.0	35.0	41.0
21	38.6767271958417	40.0	37.0	41.0	35.0	41.0
22	38.631826704603384	40.0	36.0	41.0	35.0	41.0
23	38.587178969805535	40.0	36.0	41.0	35.0	41.0
24	38.5089017491175	40.0	36.0	41.0	35.0	41.0
25	38.440511544690665	40.0	36.0	41.0	35.0	41.0
26	38.34071836492878	40.0	35.0	41.0	35.0	41.0
27	38.27509023565916	40.0	36.0	41.0	35.0	41.0
28	38.26629205765208	40.0	36.0	41.0	35.0	41.0
29	38.239373228424206	40.0	36.0	41.0	35.0	41.0
30	38.157415208532115	40.0	35.0	41.0	35.0	41.0
31	38.068092370404635	40.0	35.0	41.0	34.0	41.0
32	37.95371229984749	40.0	35.0	41.0	34.0	41.0
33	37.80118586238244	40.0	35.0	41.0	34.0	41.0
34	37.64886281067308	40.0	35.0	41.0	34.0	41.0
35	37.52421202777208	40.0	35.0	41.0	34.0	41.0
36	37.43618517011957	40.0	35.0	41.0	33.0	41.0
37	37.32341608647168	40.0	35.0	41.0	33.0	41.0
38	37.30647603858212	40.0	35.0	41.0	33.0	41.0
39	37.20681218854879	40.0	35.0	41.0	33.0	41.0
40	37.1344578401184	40.0	35.0	41.0	33.0	41.0
41	37.08196714273602	40.0	35.0	41.0	33.0	41.0
42	37.03818071173208	40.0	35.0	41.0	33.0	41.0
43	36.94980611273474	39.0	35.0	41.0	33.0	41.0
44	36.846716366489325	39.0	35.0	41.0	33.0	41.0
45	36.78811765678144	39.0	35.0	41.0	33.0	41.0
46	36.71137200059674	39.0	35.0	41.0	33.0	41.0
47	36.61069390497431	39.0	35.0	41.0	32.0	41.0
48	36.52761914165845	39.0	35.0	41.0	32.0	41.0
49	36.43733250190238	38.0	35.0	41.0	32.0	41.0
50	36.32570484702064	38.0	35.0	41.0	32.0	41.0
51	36.21194781303966	38.0	35.0	41.0	31.0	41.0
52	35.96708585240097	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	7.0
14	15.0
15	17.0
16	49.0
17	138.0
18	247.0
19	511.0
20	791.0
21	1442.0
22	2337.0
23	3840.0
24	6515.0
25	10517.0
26	14338.0
27	16356.0
28	16824.0
29	17399.0
30	19776.0
31	23584.0
32	30000.0
33	40736.0
34	96742.0
35	199206.0
36	117030.0
37	144348.0
38	239684.0
39	851865.0
40	9132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.23521374823312	21.35899249458264	23.706300236013337	19.6994935211709
2	28.71753206679639	23.807563803560488	26.265740930551544	21.20916319909158
3	28.519029715785848	24.277121946664636	26.73943118531501	20.464417152234507
4	23.82312630201765	27.617692736177013	24.868013913946896	23.69116704785844
5	22.211173444581945	38.571169451996134	22.044654711090267	17.17300239233166
6	87.88089214973914	2.8727298876172958	7.109807915838008	2.1365700468055557
7	89.33099502322031	2.2419120622242352	6.105221808534045	2.321871106021399
8	83.17210942690294	4.652167426724781	8.674053665934336	3.50166948043794
9	40.058514994198944	31.627450959347534	14.673343157384084	13.640690889069438
10	31.962795969205573	23.442113408756	26.510877113145803	18.084213508892628
11	29.890568803952235	21.498572006607088	30.446096335085276	18.164762854355406
12	22.996542978791	27.475161715824488	31.443223175887358	18.08507212949716
13	18.62342724853954	32.164571811270896	30.993091323960776	18.21890961622879
14	17.743770439195174	27.51820007362672	36.57391059827611	18.164118888902006
15	21.047313215137052	23.129682836281443	36.60380132807142	19.219202620510085
16	21.292342070155744	23.756475877590756	29.04600811181816	25.905173940435343
17	22.646279435929195	26.165765293911196	30.41282478665961	20.775130483499996
18	25.32345848086403	29.502847937217663	26.373014842330427	18.800678739587884
19	25.020526398827126	26.195817015069867	26.798139369149975	21.985517216953035
20	24.340713535187884	26.939006812081224	28.887538946493983	19.832740706236912
21	20.979374859803354	24.864633095316545	28.433596965634784	25.722395079245313
22	18.664480046193788	29.70306752943727	27.739992508535227	23.892459915833715
23	19.364953468129613	28.862370630023605	31.388486112348357	20.384189789498425
24	23.21125179371211	26.747373425906947	28.94517385457328	21.09620092580767
25	19.51097263468806	29.19567641594587	26.865970396908107	24.42738055245796
26	18.72651538487132	28.548544799066676	30.529704516451705	22.195235299610296
27	19.67657908378742	26.77179044934836	32.97677323937162	20.5748572274926
28	19.60348900482652	29.105682243833225	29.675967316606688	21.614861434733566
29	21.637561216965914	28.130289237083396	30.045603486858276	20.186546059092418
30	25.88403040804871	27.248002902137642	26.694675586303713	20.173291103509932
31	24.82637081462703	29.2380708082947	26.231342442582427	19.704215934495835
32	23.511447022572064	29.355809158691333	25.00292467643419	22.129819142302413
33	20.902903962212108	27.761458023648554	28.316717235842688	23.018920778296646
34	20.08072106958369	29.21274150046097	28.026235152571516	22.680302277383827
35	25.885050020016592	28.8234107200929	25.722395079245313	19.56914418064519
36	22.083775612384315	31.404960895197842	25.357052012016396	21.154211480401447
37	22.241117838165042	31.462220156762655	25.411037782526424	20.88562422254587
38	21.00202097824792	29.385002259245464	24.695216517284567	24.917760245222045
39	24.174194801696206	26.833879451813676	27.641090147650544	21.350835598839573
40	20.32467664884671	26.40628639075609	29.42600139311193	23.843035567285266
41	21.516120065212235	29.691315159912723	25.998173284663856	22.79439149021119
42	21.68247780734056	26.33931398360249	27.772190781205225	24.20601742785172
43	22.0542068653157	27.692607383922546	29.752760196924637	20.500425553837122
44	21.795064434109992	30.07377697544452	25.648661034831015	22.482497555614465
45	23.084873573482362	28.339578009438387	26.722849074889965	21.85269934218929
46	23.286112777669853	28.373761842256368	27.292865828724512	21.047259551349267
47	21.700508840035763	29.243866497375304	28.045393124810165	21.010231537778772
48	21.147020532838482	28.058648080392643	28.13587027101286	22.65846111575601
49	20.115012229977236	28.467888126028335	29.443012813839246	21.974086830155183
50	19.692302573607932	28.780533353654018	29.098705951421394	22.428458121316652
51	20.458084825276075	26.774151656010826	28.485489848421263	24.282273670291836
52	21.48966381783505	25.722985380910934	27.911501974290754	24.875848826963264
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3148.0
1	3115.5
2	3083.0
3	14128.5
4	25174.0
5	15500.5
6	5827.0
7	6596.5
8	7366.0
9	8764.5
10	10163.0
11	11298.0
12	12433.0
13	12719.0
14	12063.0
15	11121.0
16	10037.0
17	8953.0
18	8653.0
19	8353.0
20	8024.5
21	7696.0
22	8066.5
23	8437.0
24	9181.5
25	9926.0
26	10943.0
27	11960.0
28	14413.0
29	16866.0
30	19635.5
31	22405.0
32	24456.0
33	26507.0
34	32521.5
35	38536.0
36	44216.0
37	49896.0
38	53303.0
39	62401.0
40	68092.0
41	82694.5
42	97297.0
43	113321.0
44	129345.0
45	144485.5
46	159626.0
47	159694.5
48	159763.0
49	167742.0
50	175721.0
51	156350.5
52	136980.0
53	123201.5
54	109423.0
55	102665.5
56	95908.0
57	91962.5
58	88017.0
59	83047.5
60	78078.0
61	71988.5
62	65899.0
63	56245.0
64	39281.5
65	31972.0
66	26738.0
67	21504.0
68	17956.5
69	14409.0
70	12074.5
71	9740.0
72	8853.5
73	7967.0
74	6380.5
75	4794.0
76	3595.0
77	2396.0
78	1706.5
79	1017.0
80	837.5
81	658.0
82	509.5
83	361.0
84	296.5
85	232.0
86	151.0
87	70.0
88	44.0
89	11.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1863454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.12848334139925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8007890437487	15.592987418929644
2	10.268642420345358	4.339216806341051
3	4.0071661034476795	2.5399602678874214
4	1.9837939351021814	1.676582284423012
5	1.1930057155046099	1.260320069311662
6	0.8419147240570293	1.0673028733270613
7	0.6112605763787453	0.9040506233690697
8	0.4720564992538056	0.7979070304546624
9	0.40282050468266617	0.7659887690485574
>10	4.197060419243832	20.30932873933353
>50	1.3260279660600143	20.219951065147907
>100	0.8870504073161984	26.307293120407465
>500	0.0040783926590308335	0.5535361521482203
>1k	0.0033136940354625516	1.2091840521533217
>5k	5.097990823788542E-4	0.8526546371677977
>10k+	5.097990823788542E-4	1.603736090549689
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19489	1.0458535601093453	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10289	0.5521467125026965	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7976	0.42802237135985116	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7856	0.4215827168258513	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3290	0.17655386180716023	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3082	0.1653917939482273	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2650	0.14220903762582818	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT	2380	0.1277198149243287	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.3663787783331384E-5	0.0	0.0	0.13254955582482852	0.0
2	5.3663787783331384E-5	0.0	0.0	0.3277784157805881	0.0
3	5.3663787783331384E-5	0.0	0.0	0.5969559753017784	0.0
4	5.3663787783331384E-5	0.0	0.0	0.7840816033022548	0.0
5	5.3663787783331384E-5	0.0	0.0	1.7956976668058346	0.0
6	5.3663787783331384E-5	0.0	0.0	2.1471954767866555	0.0
7	5.3663787783331384E-5	0.0	0.0	2.9595042324629426	0.0
8	5.3663787783331384E-5	0.0	0.0	3.934253273759374	0.0
9	5.3663787783331384E-5	0.0	0.0	4.882707059041973	0.0
10	5.3663787783331384E-5	0.0	0.0	5.540571433477832	0.0
11	5.3663787783331384E-5	0.0	0.0	6.184912533392292	0.0
12	1.0732757556666277E-4	0.0	0.0	6.650123909685992	0.0
13	1.0732757556666277E-4	0.0	0.0	6.865852336574984	0.0
14	1.0732757556666277E-4	0.0	0.0	6.988796074386596	0.0
15	1.0732757556666277E-4	0.0	0.0	7.342869746181017	0.0
16	1.0732757556666277E-4	0.0	0.0	7.786025305695767	0.0
17	1.0732757556666277E-4	0.0	0.0	8.322716847316864	0.0
18	1.6099136334999414E-4	0.0	0.0	8.609603456806553	0.0
19	1.6099136334999414E-4	0.0	0.0	8.91076463384661	0.0
20	1.6099136334999414E-4	0.0	0.0	9.252388306875297	0.0
21	1.6099136334999414E-4	0.0	0.0	9.614296891686084	0.0
22	1.6099136334999414E-4	0.0	0.0	9.983986725725455	0.0
23	1.6099136334999414E-4	0.0	0.0	10.26937074915721	0.0
24	1.6099136334999414E-4	0.0	0.0	10.496475899056268	0.0
25	1.6099136334999414E-4	0.0	0.0	10.73232824636401	0.0
26	1.6099136334999414E-4	0.0	0.0	10.983582100765567	0.0
27	1.6099136334999414E-4	0.0	0.0	11.254208582556908	0.0
28	1.6099136334999414E-4	0.0	0.0	11.540236571442064	0.0
29	2.1465515113332554E-4	0.0	0.0	11.825888913812737	0.0
30	2.1465515113332554E-4	0.0	0.0	12.111648583758976	0.0
31	2.1465515113332554E-4	0.0	0.0	12.351686706513817	0.0
32	2.1465515113332554E-4	0.0	0.0	12.631597023591674	0.0
33	2.1465515113332554E-4	0.0	0.0	12.936836648503263	0.0
34	2.1465515113332554E-4	0.0	0.0	13.291768940902218	0.0
35	2.1465515113332554E-4	0.0	0.0	13.56566891374834	0.0
36	2.1465515113332554E-4	0.0	0.0	13.855829014292814	0.0
37	2.1465515113332554E-4	0.0	0.0	14.194769497932334	0.0
38	2.683189389166569E-4	0.0	0.0	14.78571512900238	0.0
39	3.219827266999883E-4	0.0	0.0	15.130665956873633	0.0
40	3.219827266999883E-4	0.0	0.0	15.450985106152338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	6.312469E-4	46.0	13
TAACGCG	20	6.312469E-4	46.0	45
CTGCGTA	20	6.312469E-4	46.0	36
CCCGTAG	25	3.417979E-5	46.0	44
CATACCG	20	6.312469E-4	46.0	14
TTAACGC	20	6.312469E-4	46.0	36
CTATTCG	25	3.417979E-5	46.0	20
CCGAATA	25	3.417979E-5	46.0	28
TATTAGG	255	0.0	44.19608	1
CTAACGG	115	0.0	44.0	1
ATTCGGC	100	0.0	43.7	16
GACCGAT	500	0.0	42.780003	8
TACGGGA	965	0.0	42.663208	3
CGTTTTT	6520	0.0	42.57822	1
CGCACTT	4460	0.0	42.441704	34
GCGTAGG	495	0.0	42.282825	1
CGTAGGG	1130	0.0	42.132744	2
ACCCGCA	4540	0.0	41.896477	31
CCGCACT	4525	0.0	41.832047	33
CCCGCAC	4555	0.0	41.809002	32
>>END_MODULE