FastQCFastQC Report
Thu 9 Feb 2017
SRR3211946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5289107
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73440.13885141669472748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56580.10697458001889544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53140.10047064655715984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC47550.020.81493237
TACTTAC12550.016.3625531
TCTTATA81550.015.69834437
TATACCG2304.0017767E-1113.6739135
CTCGTTA11350.013.52863422
TAGATCG1755.315469E-712.6857155
CTCTTAT126800.012.67862737
GGAGTGT19900.012.6432166
TACGGAT16450.012.48328227
AGTGTAC17200.012.36918527
GGTATCA69450.012.2267811
TAAGACT7900.012.1772154
GTACTTA16950.012.11504430
TCGTTAC11900.011.97058923
ACGGATG17950.011.74930428
GTCTTAG8900.011.6404491
TTACGGA18100.011.54972426
TTAGCCG5450.011.5412854
ACTTACA12400.011.48790332
GAGTGTC19700.011.3629447
Produced by FastQC (version 0.11.5)