##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211946.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5289107
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46822195126701	34.0	31.0	34.0	31.0	34.0
2	32.60165411665901	34.0	31.0	34.0	31.0	34.0
3	32.67926665125134	34.0	31.0	34.0	31.0	34.0
4	36.16701855341554	37.0	35.0	37.0	35.0	37.0
5	36.05673471911232	37.0	35.0	37.0	35.0	37.0
6	36.075589697845025	37.0	35.0	37.0	35.0	37.0
7	36.06239314122403	37.0	35.0	37.0	35.0	37.0
8	36.038138952378915	37.0	35.0	37.0	35.0	37.0
9	37.87921590544491	39.0	38.0	39.0	35.0	39.0
10	37.752030541261504	39.0	37.0	39.0	35.0	39.0
11	37.851938143811424	39.0	38.0	39.0	35.0	39.0
12	37.7893137726274	39.0	38.0	39.0	35.0	39.0
13	37.8400493693926	39.0	38.0	39.0	35.0	39.0
14	39.20887968422647	40.0	39.0	41.0	36.0	41.0
15	39.17342020117952	40.0	39.0	41.0	36.0	41.0
16	39.130531486695205	40.0	39.0	41.0	36.0	41.0
17	39.12023126021084	40.0	39.0	41.0	36.0	41.0
18	39.10880570198334	40.0	38.0	41.0	36.0	41.0
19	39.12192644240323	40.0	39.0	41.0	36.0	41.0
20	39.07859512012141	40.0	39.0	41.0	36.0	41.0
21	39.066056141424255	40.0	39.0	41.0	36.0	41.0
22	39.038019650576175	40.0	39.0	41.0	36.0	41.0
23	38.98023919727848	40.0	39.0	41.0	36.0	41.0
24	38.94701128186667	40.0	38.0	41.0	35.0	41.0
25	38.89767006037125	40.0	38.0	41.0	35.0	41.0
26	38.799622129028585	40.0	38.0	41.0	35.0	41.0
27	38.71654080736124	40.0	38.0	41.0	35.0	41.0
28	38.65304937109421	40.0	38.0	41.0	35.0	41.0
29	38.591419496712774	40.0	38.0	41.0	35.0	41.0
30	38.53503587656669	40.0	38.0	41.0	34.0	41.0
31	38.48395655448075	40.0	38.0	41.0	34.0	41.0
32	38.419331656553744	40.0	38.0	41.0	34.0	41.0
33	38.38133374877839	40.0	38.0	41.0	34.0	41.0
34	38.32639195992821	40.0	38.0	41.0	34.0	41.0
35	38.27334935746242	40.0	38.0	41.0	34.0	41.0
36	38.21182157971091	40.0	38.0	41.0	34.0	41.0
37	38.18205417284997	40.0	38.0	41.0	34.0	41.0
38	38.125410584433254	40.0	38.0	41.0	34.0	41.0
39	38.080389184790555	40.0	38.0	41.0	33.0	41.0
40	38.02987290671185	40.0	38.0	41.0	33.0	41.0
41	37.946984245166526	40.0	38.0	41.0	33.0	41.0
42	37.85942428466658	40.0	37.0	41.0	33.0	41.0
43	36.92999404247258	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	2.0
13	2.0
14	1.0
15	3.0
16	19.0
17	43.0
18	78.0
19	193.0
20	451.0
21	949.0
22	1711.0
23	3138.0
24	5271.0
25	8303.0
26	12325.0
27	18212.0
28	26197.0
29	35954.0
30	49206.0
31	65426.0
32	86074.0
33	115858.0
34	161132.0
35	227698.0
36	343495.0
37	594242.0
38	1393435.0
39	2139680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.670133162365595	17.351908365627693	12.907604251530552	27.070354220476162
2	18.175034084203627	19.49924628108299	36.55823185274944	25.76748778196395
3	19.923798100511107	21.46971123858905	29.4442332136597	29.162257447240147
4	15.249795475871448	14.974871939629885	33.68988375542412	36.08544882907455
5	16.120225966311516	34.822059754132404	33.463872067628806	15.593842211927269
6	36.166540022729734	33.636869134997646	14.94494250163591	15.25164834063671
7	30.36627922256063	29.69287632108785	20.499301677958112	19.4415427783934
8	26.98480102595769	35.0846749744333	19.152079925779532	18.778444073829476
9	27.722902939948085	14.635476272270537	18.14364882389409	39.49797196388729
10	17.124875711533157	26.01446709246003	32.5666317584424	24.29402543756441
11	37.38956311528581	20.910013731996724	20.562582681726802	21.13784047099066
12	22.264382248269886	23.73998105918447	29.52419000031574	24.471446692229897
13	31.786462251567233	19.14865779799879	23.359954714472593	25.704925235961383
14	23.031903117104648	19.487221566892103	24.831261685573764	32.649613630429485
15	26.457434118840855	26.156135619869293	21.66136930109374	25.725060960196117
16	25.219512481029405	25.176953311778338	23.90352473489381	25.70000947229844
17	24.989549275520424	24.93222390849722	24.595569724719127	25.482657091263235
18	25.207355419355288	24.74464971118943	25.008607313105973	25.0393875563493
19	26.382336375497793	24.55658393751535	24.806909748658896	24.254169938327962
20	25.69288161498718	24.12467737937614	24.811787698755197	25.370653306881486
21	25.30260779371641	24.703395109987376	25.08587933653072	24.908117759765496
22	25.923430930779052	24.171982151240275	24.265948864335698	25.638638053644975
23	25.367155551967468	24.279788629725207	24.738580633744036	25.61447518456329
24	25.156155850127444	24.05629154411132	24.840941958633092	25.946610647128143
25	25.650492606785985	23.914623016702063	24.61097875312411	25.823905623387844
26	25.259708302365596	24.188468866294443	25.03745906445077	25.514363766889193
27	25.901234367162544	24.502075681206676	24.558644776897122	25.038045174733654
28	25.395269938762823	24.934700697112007	24.18173805143288	25.488291312692297
29	25.314878296090438	24.86531280233128	24.708348687216954	25.111460214361326
30	25.822204012889134	24.510640454050183	24.486345237485267	25.18081029557542
31	25.416294281813546	24.05818222244322	24.761476748343338	25.764046747399895
32	24.72296363072254	24.472070616079424	24.843759069347623	25.961206683850413
33	25.067085993911636	23.937367877034816	25.023543671927982	25.97200245712556
34	25.427298029705202	23.94319116629707	25.54291300970088	25.086597794296843
35	25.246454647258982	23.848487088652206	25.318413864571088	25.586644399517727
36	24.909460141381146	23.39566962816218	25.895751400000037	25.799118830456635
37	24.480975711022673	23.039522550782202	26.20771332476352	26.271788413431608
38	24.636748698787905	22.597708838183834	26.552213823619	26.213328639409262
39	23.904980557209374	22.600922991348067	27.484753097261976	26.00934335418058
40	24.534179399282337	22.394366383588004	27.60293939978904	25.46851481734062
41	23.64669499028853	22.497408352676548	28.041501145656532	25.814395511378386
42	23.031165752555204	22.816876270417673	28.228300164848243	25.92365781217888
43	22.72423303215458	22.52382112897319	28.03401405946221	26.71793177941002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	163.0
1	207.5
2	252.0
3	789.0
4	1326.0
5	1326.0
6	1669.5
7	2013.0
8	1930.5
9	1848.0
10	2455.5
11	3063.0
12	3063.0
13	5222.0
14	7381.0
15	11644.0
16	15907.0
17	15335.0
18	14763.0
19	14763.0
20	17517.5
21	20272.0
22	20945.0
23	21618.0
24	27313.5
25	33009.0
26	33009.0
27	41645.5
28	50282.0
29	59733.5
30	69185.0
31	82367.0
32	95549.0
33	95549.0
34	112243.5
35	128938.0
36	147728.5
37	166519.0
38	190127.0
39	213735.0
40	213735.0
41	240646.5
42	267558.0
43	296602.0
44	325646.0
45	362822.0
46	399998.0
47	399998.0
48	435225.5
49	470453.0
50	491020.0
51	511587.0
52	512188.5
53	512790.0
54	512790.0
55	487053.0
56	461316.0
57	429201.0
58	397086.0
59	364671.0
60	332256.0
61	332256.0
62	291706.0
63	251156.0
64	212491.0
65	173826.0
66	146177.0
67	118528.0
68	118528.0
69	98337.5
70	78147.0
71	65331.0
72	52515.0
73	43566.0
74	34617.0
75	34617.0
76	28816.0
77	23015.0
78	18689.5
79	14364.0
80	11662.0
81	8960.0
82	8960.0
83	6963.0
84	4966.0
85	3847.5
86	2729.0
87	1963.5
88	1198.0
89	1198.0
90	805.5
91	413.0
92	262.5
93	112.0
94	71.5
95	31.0
96	31.0
97	22.0
98	13.0
99	8.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5289107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.575489558379765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56636715372362	45.58081663662081
2	10.243669390368195	11.590812212685403
3	3.320766004012975	5.636218871575758
4	1.6244624673343506	3.676190374346175
5	0.961679650326611	2.7203748507779735
6	0.6298232359452041	2.1379534745305713
7	0.4476385193870922	1.772775786565911
8	0.3365132087858968	1.5230719623938702
9	0.2544149897643199	1.295428733721592
>10	1.4870603584026338	15.3949120396897
>50	0.08664381335077816	3.3287219676203192
>100	0.03858421770237584	3.958988921342739
>500	0.001807854228213904	0.6602752897723083
>1k	4.687007851871253E-4	0.376541064564948
>5k	1.0043588253239644E-4	0.3469178137919799
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7344	0.13885141669472748	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5658	0.10697458001889544	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5314	0.10047064655715984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.672034995699652E-5
2	0.0	0.0	0.0	0.0	5.672034995699652E-5
3	0.0	0.0	0.0	0.0	5.672034995699652E-5
4	0.0	0.0	0.0	0.0	5.672034995699652E-5
5	0.0	0.0	0.0	0.0	7.562713327599536E-5
6	0.0	0.0	0.0	0.0	9.45339165949942E-5
7	0.0	0.0	0.0	0.0	9.45339165949942E-5
8	0.0	0.0	0.0	0.0	9.45339165949942E-5
9	0.0	0.0	0.0	0.0	9.45339165949942E-5
10	1.890678331899884E-5	0.0	0.0	1.890678331899884E-5	9.45339165949942E-5
11	1.890678331899884E-5	0.0	0.0	3.781356663799768E-5	9.45339165949942E-5
12	1.890678331899884E-5	0.0	0.0	9.45339165949942E-5	1.512542665519907E-4
13	1.890678331899884E-5	0.0	0.0	9.45339165949942E-5	1.512542665519907E-4
14	1.890678331899884E-5	0.0	0.0	1.323474832329919E-4	1.890678331899884E-4
15	1.890678331899884E-5	0.0	0.0	1.512542665519907E-4	2.457881831469849E-4
16	1.890678331899884E-5	0.0	0.0	2.2688139982798608E-4	2.836017497849826E-4
17	1.890678331899884E-5	0.0	0.0	3.59228883060978E-4	3.025085331039814E-4
18	1.890678331899884E-5	0.0	0.0	3.781356663799768E-4	3.9704244969897566E-4
19	1.890678331899884E-5	0.0	0.0	5.482967162509664E-4	3.9704244969897566E-4
20	1.890678331899884E-5	0.0	0.0	6.239238495269618E-4	4.3485601633697334E-4
21	1.890678331899884E-5	0.0	0.0	8.129916827169501E-4	4.3485601633697334E-4
22	1.890678331899884E-5	0.0	0.0	0.0012478476990539235	4.3485601633697334E-4
23	1.890678331899884E-5	0.0	0.0	0.0015881697987959026	4.5376279965597216E-4
24	1.890678331899884E-5	0.0	0.0	0.0026658564479788366	4.5376279965597216E-4
25	3.781356663799768E-5	0.0	0.0	0.003686822747204774	4.5376279965597216E-4
26	3.781356663799768E-5	0.0	0.0	0.006087984228717627	4.5376279965597216E-4
27	3.781356663799768E-5	0.0	0.0	0.020721834517622727	4.5376279965597216E-4
28	3.781356663799768E-5	0.0	0.0	0.07543806544280537	4.5376279965597216E-4
29	3.781356663799768E-5	0.0	0.0	0.16233364157692404	4.5376279965597216E-4
30	3.781356663799768E-5	0.0	0.0	0.2819190460695917	4.5376279965597216E-4
31	3.781356663799768E-5	0.0	0.0	0.7819467445071541	4.7266958297497103E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4755	0.0	20.814932	37
TACTTAC	1255	0.0	16.36255	31
TCTTATA	8155	0.0	15.698344	37
TATACCG	230	4.0017767E-11	13.673913	5
CTCGTTA	1135	0.0	13.528634	22
TAGATCG	175	5.315469E-7	12.685715	5
CTCTTAT	12680	0.0	12.678627	37
GGAGTGT	1990	0.0	12.643216	6
TACGGAT	1645	0.0	12.483282	27
AGTGTAC	1720	0.0	12.369185	27
GGTATCA	6945	0.0	12.226781	1
TAAGACT	790	0.0	12.177215	4
GTACTTA	1695	0.0	12.115044	30
TCGTTAC	1190	0.0	11.970589	23
ACGGATG	1795	0.0	11.749304	28
GTCTTAG	890	0.0	11.640449	1
TTACGGA	1810	0.0	11.549724	26
TTAGCCG	545	0.0	11.541285	4
ACTTACA	1240	0.0	11.487903	32
GAGTGTC	1970	0.0	11.362944	7
>>END_MODULE