FastQCFastQC Report
Thu 9 Feb 2017
SRR3211945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893222
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7500.020.96666537
TTAGACA701.2188108E-418.54
TCTTATA14450.018.30795937
TACTTAC1950.018.0256431
CCCTATA1054.794292E-717.619052
TTAGACT852.7220156E-517.4117644
CGTATTA752.0666164E-417.26666614
TCTAGTA650.001579561317.0769232
GCTCTAG650.001579561317.0769231
CTTAGAC1005.874839E-616.6500023
CTAGATC904.444171E-516.4444454
CGCGGTA904.444171E-516.44444522
CGATAGT904.444171E-516.44444528
TCGTTAC2300.016.08695623
ACTTACA1851.8189894E-1116.032
TCTAGCA1059.338351E-615.8571442
TAGATCA700.002591886315.8571435
TGAATAG700.002591886315.8571435
GGACCGT1302.5868758E-715.6538466
TTAAACA957.0568596E-515.5789484
Produced by FastQC (version 0.11.5)