##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211945.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893222
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54073679331678	34.0	31.0	34.0	31.0	34.0
2	32.66304345392299	34.0	31.0	34.0	31.0	34.0
3	32.74248730998565	34.0	31.0	34.0	31.0	34.0
4	36.20510018785923	37.0	35.0	37.0	35.0	37.0
5	36.10758019842771	37.0	35.0	37.0	35.0	37.0
6	36.13342707635952	37.0	36.0	37.0	35.0	37.0
7	36.10985175018081	37.0	35.0	37.0	35.0	37.0
8	36.09351426633021	37.0	35.0	37.0	35.0	37.0
9	37.95005273045223	39.0	38.0	39.0	35.0	39.0
10	37.82354330726292	39.0	38.0	39.0	35.0	39.0
11	37.928810530864666	39.0	38.0	39.0	35.0	39.0
12	37.86006838165652	39.0	38.0	39.0	35.0	39.0
13	37.91564135231779	39.0	38.0	39.0	35.0	39.0
14	39.2940646334282	40.0	39.0	41.0	36.0	41.0
15	39.26760648528585	40.0	39.0	41.0	36.0	41.0
16	39.21996883193652	40.0	39.0	41.0	36.0	41.0
17	39.201748277583846	40.0	39.0	41.0	36.0	41.0
18	39.18852312191146	40.0	39.0	41.0	36.0	41.0
19	39.20271668185513	40.0	39.0	41.0	36.0	41.0
20	39.15813090138845	40.0	39.0	41.0	36.0	41.0
21	39.14470982577679	40.0	39.0	41.0	36.0	41.0
22	39.127838320148854	40.0	39.0	41.0	36.0	41.0
23	39.069554937070514	40.0	39.0	41.0	36.0	41.0
24	39.040172543891664	40.0	39.0	41.0	36.0	41.0
25	38.98191043212102	40.0	38.0	41.0	35.0	41.0
26	38.891300259062135	40.0	38.0	41.0	35.0	41.0
27	38.80809585970789	40.0	38.0	41.0	35.0	41.0
28	38.7329510468842	40.0	38.0	41.0	35.0	41.0
29	38.67486918145769	40.0	38.0	41.0	35.0	41.0
30	38.63188658586555	40.0	38.0	41.0	35.0	41.0
31	38.5909650680346	40.0	38.0	41.0	35.0	41.0
32	38.534675590166835	40.0	38.0	41.0	35.0	41.0
33	38.50039407896357	40.0	38.0	41.0	34.0	41.0
34	38.455539608294465	40.0	38.0	41.0	34.0	41.0
35	38.41058102017192	40.0	38.0	41.0	34.0	41.0
36	38.336694573129634	40.0	38.0	41.0	34.0	41.0
37	38.32932462478533	40.0	38.0	41.0	34.0	41.0
38	38.28322522284493	40.0	38.0	41.0	34.0	41.0
39	38.23881409100985	40.0	38.0	41.0	34.0	41.0
40	38.19362711621523	40.0	38.0	41.0	34.0	41.0
41	38.12611310514072	40.0	38.0	41.0	33.0	41.0
42	38.046360255345256	40.0	38.0	41.0	33.0	41.0
43	37.07063081742277	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	2.0
16	3.0
17	2.0
18	12.0
19	22.0
20	64.0
21	118.0
22	235.0
23	477.0
24	806.0
25	1253.0
26	1868.0
27	2746.0
28	3928.0
29	5573.0
30	7773.0
31	10362.0
32	13807.0
33	18733.0
34	25884.0
35	37012.0
36	55429.0
37	94349.0
38	225910.0
39	386853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.572034723730496	15.986507273667689	12.197751510822616	28.243706491779196
2	18.941427774954043	18.624597244581974	34.871510106110236	27.562464874353743
3	20.51785558349436	20.3351462458381	28.767540432277755	30.379457738389785
4	16.043268078932226	14.021374305603759	31.958236586201416	37.9771210292626
5	17.05611818786371	33.983488987060326	32.94343399513223	16.016958829943732
6	38.646607450331494	31.60882736878402	14.059438750948813	15.685126429935673
7	31.758958019394957	28.650436285716207	19.407829184681972	20.182776510206867
8	28.513964053729087	34.0050961575062	18.417817742957517	19.0631220458072
9	28.685142103530815	13.58352122988462	16.684765937247402	41.04657072933716
10	17.553642879373772	25.14044660789815	31.77015344449644	25.53575706823164
11	39.37520571593624	19.675063981854453	19.315131064841662	21.63459923736764
12	23.187740561696867	22.443916517954104	28.647861337942864	25.720481582406162
13	33.43659247085271	17.66817207816198	22.25191497746361	26.643320473521698
14	23.930668971431516	18.1081522846504	23.578460897738747	34.38271784617934
15	28.12425130594634	24.638107883594447	20.483037811428737	26.754602999030475
16	26.576035968661767	23.861033427300267	22.78213031027001	26.780800293767953
17	26.4632980378898	23.496734294497895	23.166581208255057	26.873386459357246
18	26.53394117028018	23.645185631343608	23.620443741869323	26.20042945650689
19	28.055847258576254	23.338095120809832	23.24830781149591	25.357749809118
20	27.225146716045955	22.508849983542724	23.13836873699931	27.127634563412006
21	26.44695271724163	23.5886487345811	23.68784020098027	26.276558347197
22	27.539066435891634	22.81246991229504	22.338567567749116	27.309896084064206
23	26.68451963789517	22.94278466047634	23.26789980542351	27.104795896204976
24	26.390751683232168	22.536502683543397	23.542075766158916	27.530669867065523
25	27.210032892158946	22.329835136169958	22.898898594078513	27.561233377592583
26	26.638618394979076	22.712494766138764	23.78378499410001	26.865101844782146
27	27.738009139945053	22.92341657505077	23.165237757242878	26.173336527761297
28	26.662688558947274	23.988661273457215	22.43551994912799	26.913130218467522
29	26.870475648830862	23.5999561139336	23.177552724854518	26.352015512381023
30	27.603328175974166	23.00503122404061	22.85691574994794	26.534724850037282
31	26.878536354903932	22.701859112292354	23.39048971028479	27.029114822518924
32	26.09284142128161	22.924871980313966	23.38086164469751	27.601424953706914
33	26.311711982015666	22.57109654710699	23.58976827709125	27.527423193786092
34	26.742847802673914	22.70085152403322	24.36158088358773	26.194719789705132
35	26.602904988905333	22.389842614713924	24.048332889248137	26.958919507132606
36	26.07951886541084	22.155858230092853	24.788126579954366	26.976496324541937
37	25.462315079565883	21.653183643036108	25.214672276321004	27.669829001077
38	25.956257235043473	21.04851873330482	25.35371945608147	27.64150457557024
39	24.72117793784748	21.171780363672188	26.66503959821858	27.442002100261746
40	25.720033765402107	20.67683061993547	26.871259328587964	26.731876286074456
41	24.78174518764652	20.772887367306225	27.381098987709663	27.064268457337594
42	23.741802149969438	21.00474462115801	28.13466305129072	27.11879017758183
43	23.33093004874488	20.616151415885412	27.8211911484491	28.231727386920607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	20.5
4	38.0
5	38.0
6	46.0
7	54.0
8	54.5
9	55.0
10	72.0
11	89.0
12	89.0
13	144.5
14	200.0
15	316.0
16	432.0
17	430.0
18	428.0
19	428.0
20	553.0
21	678.0
22	806.5
23	935.0
24	1295.5
25	1656.0
26	1656.0
27	2248.5
28	2841.0
29	3680.5
30	4520.0
31	5759.0
32	6998.0
33	6998.0
34	8998.0
35	10998.0
36	13694.5
37	16391.0
38	20223.5
39	24056.0
40	24056.0
41	28873.0
42	33690.0
43	39775.0
44	45860.0
45	54279.0
46	62698.0
47	62698.0
48	72144.0
49	81590.0
50	88761.5
51	95933.0
52	98855.0
53	101777.0
54	101777.0
55	98283.0
56	94789.0
57	88897.0
58	83005.0
59	77204.5
60	71404.0
61	71404.0
62	62508.0
63	53612.0
64	45048.5
65	36485.0
66	30252.0
67	24019.0
68	24019.0
69	19583.5
70	15148.0
71	12300.0
72	9452.0
73	7567.0
74	5682.0
75	5682.0
76	4567.0
77	3452.0
78	2752.5
79	2053.0
80	1605.5
81	1158.0
82	1158.0
83	873.5
84	589.0
85	431.5
86	274.0
87	201.0
88	128.0
89	128.0
90	79.0
91	30.0
92	20.5
93	11.0
94	9.0
95	7.0
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	893222.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.14944631425969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.66037047091821	62.524977468653695
2	7.660606553600586	11.05417042547343
3	2.345601637491317	5.077015782564582
4	1.0720885304922916	3.0940237549954865
5	0.6166132180655789	2.2244151136742696
6	0.4118240399214118	1.7827725875538867
7	0.26797351049373536	1.3533898286308026
8	0.19301752150892726	1.114088584465585
9	0.12115995418231962	0.7867461248743889
>10	0.6042546568168945	7.4226718024333005
>50	0.03218780024821011	1.6072704815248722
>100	0.0136802756056284	1.687923678304252
>500	6.218306549326631E-4	0.2705343668513895
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1195425101486528E-4
2	0.0	0.0	0.0	0.0	1.1195425101486528E-4
3	0.0	0.0	0.0	1.1195425101486528E-4	2.2390850202973057E-4
4	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
5	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
6	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
7	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
8	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
9	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
10	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
11	0.0	0.0	0.0	1.1195425101486528E-4	3.3586275304459584E-4
12	0.0	0.0	0.0	1.1195425101486528E-4	4.4781700405946114E-4
13	0.0	0.0	0.0	1.1195425101486528E-4	4.4781700405946114E-4
14	0.0	0.0	0.0	1.1195425101486528E-4	6.717255060891917E-4
15	0.0	0.0	0.0	1.1195425101486528E-4	7.83679757104057E-4
16	0.0	0.0	0.0	1.1195425101486528E-4	7.83679757104057E-4
17	0.0	0.0	0.0	1.1195425101486528E-4	7.83679757104057E-4
18	0.0	0.0	0.0	1.1195425101486528E-4	8.956340081189223E-4
19	0.0	0.0	0.0	1.1195425101486528E-4	8.956340081189223E-4
20	0.0	0.0	0.0	3.3586275304459584E-4	8.956340081189223E-4
21	0.0	0.0	0.0	6.717255060891917E-4	0.0010075882591337876
22	0.0	0.0	0.0	0.0014554052631932488	0.0010075882591337876
23	0.0	0.0	0.0	0.0019032222672527097	0.0011195425101486528
24	0.0	0.0	0.0	0.003134719028416228	0.0011195425101486528
25	0.0	0.0	0.0	0.004366215789579746	0.0011195425101486528
26	0.0	0.0	0.0	0.006829209311906783	0.0011195425101486528
27	0.0	0.0	0.0	0.018248542915423042	0.0011195425101486528
28	0.0	0.0	0.0	0.06605300809877052	0.0011195425101486528
29	0.0	0.0	0.0	0.14598834332338434	0.0011195425101486528
30	0.0	0.0	0.0	0.25727086883216044	0.0011195425101486528
31	0.0	0.0	0.0	0.779537449816507	0.0011195425101486528
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	750	0.0	20.966665	37
TTAGACA	70	1.2188108E-4	18.5	4
TCTTATA	1445	0.0	18.307959	37
TACTTAC	195	0.0	18.02564	31
CCCTATA	105	4.794292E-7	17.61905	2
TTAGACT	85	2.7220156E-5	17.411764	4
CGTATTA	75	2.0666164E-4	17.266666	14
TCTAGTA	65	0.0015795613	17.076923	2
GCTCTAG	65	0.0015795613	17.076923	1
CTTAGAC	100	5.874839E-6	16.650002	3
CTAGATC	90	4.444171E-5	16.444445	4
CGCGGTA	90	4.444171E-5	16.444445	22
CGATAGT	90	4.444171E-5	16.444445	28
TCGTTAC	230	0.0	16.086956	23
ACTTACA	185	1.8189894E-11	16.0	32
TCTAGCA	105	9.338351E-6	15.857144	2
TAGATCA	70	0.0025918863	15.857143	5
TGAATAG	70	0.0025918863	15.857143	5
GGACCGT	130	2.5868758E-7	15.653846	6
TTAAACA	95	7.0568596E-5	15.578948	4
>>END_MODULE