FastQCFastQC Report
Thu 9 Feb 2017
SRR3211944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3808151
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49320.12951167114959464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC30150.020.61691737
TACTTAC10700.018.67289731
TCTTATA53000.015.95188637
AGTGTAC15350.014.58306127
TATCGCG4250.014.3647067
GTACTTA14500.014.28965530
GTGTACT15450.013.77022628
GGAGTGT19500.013.756416
TCTATAC3900.013.7564093
TCGATTA1356.576058E-613.70370313
GTATTAG3800.013.6315791
GTATCGC5900.013.483056
CTACAGT17550.013.38746523
ACTTACA10100.013.18811832
TTATACT5900.013.1694924
GACGTAT1857.397648E-813.028
TGTATCG6300.012.9206355
TAATACG2900.012.7586227
CAGTGTA18950.012.59366826
ACAGCTA18900.012.43121719
Produced by FastQC (version 0.11.5)