##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211944.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3808151
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61513238314342	34.0	31.0	34.0	31.0	34.0
2	32.738086016021946	34.0	31.0	34.0	31.0	34.0
3	32.81728902031458	34.0	31.0	34.0	31.0	34.0
4	36.25947185392596	37.0	37.0	37.0	35.0	37.0
5	36.17037533438143	37.0	35.0	37.0	35.0	37.0
6	36.19069832052353	37.0	37.0	37.0	35.0	37.0
7	36.173925088579736	37.0	36.0	37.0	35.0	37.0
8	36.15406636974217	37.0	36.0	37.0	35.0	37.0
9	38.00798130116164	39.0	38.0	39.0	35.0	39.0
10	37.898759266636226	39.0	38.0	39.0	35.0	39.0
11	37.988460279017296	39.0	38.0	39.0	35.0	39.0
12	37.93311032046786	39.0	38.0	39.0	35.0	39.0
13	37.97940470322737	39.0	38.0	39.0	35.0	39.0
14	39.390277591408534	40.0	39.0	41.0	37.0	41.0
15	39.36299348423947	40.0	39.0	41.0	36.0	41.0
16	39.32181628302029	40.0	39.0	41.0	36.0	41.0
17	39.31575428600389	40.0	39.0	41.0	36.0	41.0
18	39.303167337639714	40.0	39.0	41.0	36.0	41.0
19	39.31778466767731	40.0	39.0	41.0	36.0	41.0
20	39.279892525270135	40.0	39.0	41.0	36.0	41.0
21	39.26828190373754	40.0	39.0	41.0	36.0	41.0
22	39.24176299731812	40.0	39.0	41.0	36.0	41.0
23	39.18660315728027	40.0	39.0	41.0	36.0	41.0
24	39.16027647012947	40.0	39.0	41.0	36.0	41.0
25	39.11155229926544	40.0	39.0	41.0	36.0	41.0
26	39.02555334596764	40.0	39.0	41.0	36.0	41.0
27	38.948947665152986	40.0	39.0	41.0	35.0	41.0
28	38.888359731533754	40.0	38.0	41.0	35.0	41.0
29	38.831674216699916	40.0	38.0	41.0	35.0	41.0
30	38.78047692961755	40.0	38.0	41.0	35.0	41.0
31	38.73085127139129	40.0	38.0	41.0	35.0	41.0
32	38.66780492685295	40.0	38.0	41.0	35.0	41.0
33	38.63640832519509	40.0	38.0	41.0	35.0	41.0
34	38.586629836894595	40.0	38.0	41.0	35.0	41.0
35	38.5407537673795	40.0	38.0	41.0	35.0	41.0
36	38.47266350520239	40.0	38.0	41.0	34.0	41.0
37	38.44997900555939	40.0	38.0	41.0	34.0	41.0
38	38.40183858255621	40.0	38.0	41.0	34.0	41.0
39	38.353721267880395	40.0	38.0	41.0	34.0	41.0
40	38.306306656432476	40.0	38.0	41.0	34.0	41.0
41	38.22595795177239	40.0	38.0	41.0	34.0	41.0
42	38.14416024994807	40.0	38.0	41.0	34.0	41.0
43	37.24022051646587	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	1.0
13	0.0
14	3.0
15	3.0
16	8.0
17	20.0
18	42.0
19	102.0
20	216.0
21	489.0
22	926.0
23	1792.0
24	3094.0
25	5000.0
26	7579.0
27	11174.0
28	16027.0
29	22223.0
30	30834.0
31	40902.0
32	54111.0
33	72888.0
34	102609.0
35	146665.0
36	220403.0
37	381825.0
38	908187.0
39	1781020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94013026269179	17.13642657552182	12.755297780996605	27.16814538078978
2	17.90089206021505	19.391799327285078	36.87461447825992	25.832694134239947
3	19.902572140653035	21.410731874865256	29.478032777586815	29.208663206894897
4	15.12321859085945	14.718822861803538	33.71223987704269	36.445718670294326
5	16.06945207792443	35.02983468880305	33.45550110801804	15.445212125254487
6	36.4141285363947	33.45673530277555	14.880239780407866	15.24889638042189
7	30.46060936134098	29.929931875075333	20.312902508330158	19.296556255253535
8	27.086084559146943	35.42803318460849	19.07689584787998	18.408986408364584
9	28.060862082412175	14.611684253066645	17.837764311341644	39.489689353179536
10	17.081150406063205	26.135728336402625	32.34677406436877	24.436347193165396
11	37.51558170881354	20.917684198972154	20.468437307239128	21.098296784975176
12	22.263849306395674	23.444212164906276	29.57563920128167	24.716299327416376
13	31.865306811625903	18.969284568810426	23.468397130260854	25.697011489302813
14	23.04774679365393	19.17799478014396	24.776459756979175	32.99779866922294
15	26.69925641078833	25.93610389924139	21.63251929873579	25.732120391234485
16	25.281770602058586	25.131986625530345	23.879961692695485	25.706281079715588
17	25.092623690604704	24.910330498974435	24.373403260532474	25.623642549888388
18	25.2127344740269	24.872306796658012	24.962481792344892	24.9524769369702
19	26.599286635430158	24.64235267981758	24.5615785718581	24.19678211289416
20	25.700215143779747	23.990934183019526	24.59989112826671	25.70895954493401
21	25.184610589233465	24.771811832041323	25.140836064536305	24.902741514188907
22	26.067164878703604	24.078063081007027	23.858744046651513	25.996027993637856
23	25.30724228109652	24.2641376352986	24.773439918742717	25.655180164862163
24	25.098400772448358	23.868013637064287	24.8218361089148	26.211749481572554
25	25.739893192260492	23.765286618098916	24.470852127449778	26.02396806219081
26	25.239729201914525	24.06923990146399	25.07718312640439	25.613847770217095
27	26.04815302754539	24.41331764417955	24.493251449325406	25.04527787894965
28	25.369372170378746	25.126209543686688	23.89198852671546	25.612429759219108
29	25.397443536246328	24.93244096675788	24.594639235681566	25.07547626131422
30	26.076644544819782	24.441914199305646	24.244784411122353	25.236656844752215
31	25.62955092904667	24.023916068454216	24.64828731843879	25.69824568406032
32	24.84985495585653	24.521349074655916	24.674021592105984	25.95477437738157
33	25.15971661837989	23.816361273489417	24.79972564113135	26.224196466999338
34	25.517291725039264	24.025491636229763	25.344294383284698	25.11292225544628
35	25.39232294097582	23.9231847686712	25.05966806463294	25.62482422572004
36	25.029128309250343	23.50697228129872	25.69078799658942	25.773111412861518
37	24.548685175561577	23.330219836345776	25.9800359807161	26.141059007376544
38	25.054547469362426	22.832550494977745	25.956875134415625	26.1560269012442
39	24.03607419978882	22.92364457186703	27.10572663741538	25.93455459092877
40	24.97723961050914	22.541595645760896	27.084403953519697	25.39676079021026
41	24.04655172549618	22.806133475274486	27.367979893654425	25.779334905574906
42	23.563167531959735	22.97401022175854	27.73093819021357	25.731884056068154
43	23.142622233204513	22.65934307751977	27.362071514496144	26.83596317477957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	114.0
2	148.0
3	471.5
4	795.0
5	795.0
6	1061.0
7	1327.0
8	1227.0
9	1127.0
10	1568.0
11	2009.0
12	2009.0
13	3413.0
14	4817.0
15	7646.5
16	10476.0
17	9926.0
18	9376.0
19	9376.0
20	10949.5
21	12523.0
22	12808.0
23	13093.0
24	17021.0
25	20949.0
26	20949.0
27	26176.5
28	31404.0
29	37668.0
30	43932.0
31	52814.0
32	61696.0
33	61696.0
34	73753.0
35	85810.0
36	99596.0
37	113382.0
38	130964.5
39	148547.0
40	148547.0
41	169009.5
42	189472.0
43	211816.5
44	234161.0
45	263783.0
46	293405.0
47	293405.0
48	322514.0
49	351623.0
50	367449.5
51	383276.0
52	385657.5
53	388039.0
54	388039.0
55	365817.5
56	343596.0
57	317462.0
58	291328.0
59	266709.0
60	242090.0
61	242090.0
62	212102.5
63	182115.0
64	153002.0
65	123889.0
66	103200.0
67	82511.0
68	82511.0
69	67644.5
70	52778.0
71	43754.5
72	34731.0
73	28388.0
74	22045.0
75	22045.0
76	17901.0
77	13757.0
78	11018.0
79	8279.0
80	6603.0
81	4927.0
82	4927.0
83	3744.0
84	2561.0
85	1941.5
86	1322.0
87	922.5
88	523.0
89	523.0
90	339.0
91	155.0
92	102.0
93	49.0
94	33.0
95	17.0
96	17.0
97	13.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3808151.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.409405364514754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.66708175013963	46.31049195766216
2	10.246645965693041	11.76507703742283
3	3.359657252395337	5.786277752657874
4	1.5811912378794961	3.6310099493697146
5	0.9540599815298001	2.7386008110852877
6	0.6248239291836558	2.1522462139172016
7	0.4490993956348539	1.8047770477071938
8	0.32802740275394277	1.5065486508296035
9	0.2529403176054958	1.3069037903798713
>10	1.4246230781570879	14.675223235428048
>50	0.07285784392098751	2.8239618502282795
>100	0.035926243715027006	3.776448637947756
>500	0.002196251771906328	0.8122008089050712
>1k	8.693496197028288E-4	0.9102322564591114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4932	0.12951167114959464	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.2518925851417134E-5
5	0.0	0.0	0.0	0.0	5.2518925851417134E-5
6	0.0	0.0	0.0	0.0	7.87783887771257E-5
7	0.0	0.0	0.0	0.0	7.87783887771257E-5
8	0.0	0.0	0.0	0.0	1.0503785170283427E-4
9	0.0	0.0	0.0	5.2518925851417134E-5	1.0503785170283427E-4
10	0.0	0.0	0.0	5.2518925851417134E-5	1.0503785170283427E-4
11	0.0	0.0	0.0	1.0503785170283427E-4	1.0503785170283427E-4
12	0.0	2.6259462925708567E-5	0.0	1.3129731462854284E-4	1.0503785170283427E-4
13	0.0	2.6259462925708567E-5	0.0	1.575567775542514E-4	1.0503785170283427E-4
14	0.0	2.6259462925708567E-5	0.0	1.575567775542514E-4	1.8381624047995995E-4
15	0.0	2.6259462925708567E-5	0.0	2.1007570340566853E-4	2.363351663313771E-4
16	0.0	2.6259462925708567E-5	0.0	3.151135551085028E-4	2.625946292570857E-4
17	0.0	2.6259462925708567E-5	0.0	3.938919438856285E-4	2.625946292570857E-4
18	0.0	2.6259462925708567E-5	0.0	3.938919438856285E-4	2.8885409218279423E-4
19	0.0	2.6259462925708567E-5	0.0	4.464108697370456E-4	3.151135551085028E-4
20	0.0	2.6259462925708567E-5	0.0	6.564865731427141E-4	3.4137301803421134E-4
21	0.0	2.6259462925708567E-5	0.0	0.001024119054102634	3.938919438856285E-4
22	0.0	2.6259462925708567E-5	0.0	0.0013654920721368454	4.2015140681133707E-4
23	0.0	2.6259462925708567E-5	0.0	0.001811902941873891	4.464108697370456E-4
24	0.0	2.6259462925708567E-5	0.0	0.002494648977942314	4.464108697370456E-4
25	0.0	2.6259462925708567E-5	0.0	0.003203654476936445	4.464108697370456E-4
26	0.0	2.6259462925708567E-5	0.0	0.005593265603175924	4.726703326627542E-4
27	0.0	2.6259462925708567E-5	0.0	0.01741002391974478	4.726703326627542E-4
28	0.0	2.6259462925708567E-5	0.0	0.061079510765198124	4.989297955884628E-4
29	0.0	5.2518925851417134E-5	0.0	0.13363440682893088	4.989297955884628E-4
30	2.6259462925708567E-5	5.2518925851417134E-5	0.0	0.2327113604476293	4.989297955884628E-4
31	2.6259462925708567E-5	5.2518925851417134E-5	0.0	0.6886281557637814	4.989297955884628E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3015	0.0	20.616917	37
TACTTAC	1070	0.0	18.672897	31
TCTTATA	5300	0.0	15.951886	37
AGTGTAC	1535	0.0	14.583061	27
TATCGCG	425	0.0	14.364706	7
GTACTTA	1450	0.0	14.289655	30
GTGTACT	1545	0.0	13.770226	28
GGAGTGT	1950	0.0	13.75641	6
TCTATAC	390	0.0	13.756409	3
TCGATTA	135	6.576058E-6	13.703703	13
GTATTAG	380	0.0	13.631579	1
GTATCGC	590	0.0	13.48305	6
CTACAGT	1755	0.0	13.387465	23
ACTTACA	1010	0.0	13.188118	32
TTATACT	590	0.0	13.169492	4
GACGTAT	185	7.397648E-8	13.0	28
TGTATCG	630	0.0	12.920635	5
TAATACG	290	0.0	12.75862	27
CAGTGTA	1895	0.0	12.593668	26
ACAGCTA	1890	0.0	12.431217	19
>>END_MODULE