FastQCFastQC Report
Thu 9 Feb 2017
SRR3211943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697608
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43160.11672410920789873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC30850.021.3484637
TACTTAC9850.016.34010131
TCTTATA52200.016.16092137
ACGTATA1851.8189894E-1116.029
TAATACG3100.015.516129527
CGCGGTA3200.014.45312622
AGTGTAC12350.014.38056727
CTCGTTA10300.014.36893322
TCGTTAC10100.014.1039623
TAGATCG1203.30425E-513.8750015
ACGGATG17100.013.63157928
GTACTTA12150.013.5514430
TACGGAT16650.013.44444527
GGGCGTC10100.013.37128611
ATACCGT1805.1688403E-813.3611116
ACTTACA9650.013.22797932
CGTTACG8150.012.9386524
GTTACGG13300.012.936089525
GGAGTGT16400.012.85975556
AGGGCGT10800.012.84722210
Produced by FastQC (version 0.11.5)