##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211943.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3697608
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.588007977048946	34.0	31.0	34.0	31.0	34.0
2	32.714240124967276	34.0	31.0	34.0	31.0	34.0
3	32.79521328383106	34.0	31.0	34.0	31.0	34.0
4	36.24607962769444	37.0	37.0	37.0	35.0	37.0
5	36.15108334901915	37.0	35.0	37.0	35.0	37.0
6	36.17026412751162	37.0	36.0	37.0	35.0	37.0
7	36.15627373155835	37.0	36.0	37.0	35.0	37.0
8	36.13685712493049	37.0	36.0	37.0	35.0	37.0
9	37.985901426002975	39.0	38.0	39.0	35.0	39.0
10	37.87763467625557	39.0	38.0	39.0	35.0	39.0
11	37.96865027336592	39.0	38.0	39.0	35.0	39.0
12	37.91307867140054	39.0	38.0	39.0	35.0	39.0
13	37.957551746967226	39.0	38.0	39.0	35.0	39.0
14	39.36464736121298	40.0	39.0	41.0	36.0	41.0
15	39.33596287113183	40.0	39.0	41.0	36.0	41.0
16	39.2968867981679	40.0	39.0	41.0	36.0	41.0
17	39.285678200609695	40.0	39.0	41.0	36.0	41.0
18	39.27363906612059	40.0	39.0	41.0	36.0	41.0
19	39.29086506736247	40.0	39.0	41.0	36.0	41.0
20	39.249705755720996	40.0	39.0	41.0	36.0	41.0
21	39.24018230163933	40.0	39.0	41.0	36.0	41.0
22	39.216930512915376	40.0	39.0	41.0	36.0	41.0
23	39.15750966570821	40.0	39.0	41.0	36.0	41.0
24	39.134996462577966	40.0	39.0	41.0	36.0	41.0
25	39.08241598352232	40.0	39.0	41.0	36.0	41.0
26	38.99588707077657	40.0	39.0	41.0	36.0	41.0
27	38.91738118264565	40.0	38.0	41.0	35.0	41.0
28	38.861434743758664	40.0	38.0	41.0	35.0	41.0
29	38.80223512065097	40.0	38.0	41.0	35.0	41.0
30	38.751599141931756	40.0	38.0	41.0	35.0	41.0
31	38.70640478925835	40.0	38.0	41.0	35.0	41.0
32	38.64858578843403	40.0	38.0	41.0	35.0	41.0
33	38.616855815976166	40.0	38.0	41.0	35.0	41.0
34	38.568459122762604	40.0	38.0	41.0	35.0	41.0
35	38.521679691303135	40.0	38.0	41.0	35.0	41.0
36	38.45578168372634	40.0	38.0	41.0	34.0	41.0
37	38.43471806638237	40.0	38.0	41.0	34.0	41.0
38	38.38343356029087	40.0	38.0	41.0	34.0	41.0
39	38.342474648475445	40.0	38.0	41.0	34.0	41.0
40	38.29398059502251	40.0	38.0	41.0	34.0	41.0
41	38.21498114456698	40.0	38.0	41.0	34.0	41.0
42	38.132238733797635	40.0	38.0	41.0	34.0	41.0
43	37.21467067358141	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	1.0
14	3.0
15	5.0
16	8.0
17	20.0
18	43.0
19	91.0
20	206.0
21	476.0
22	978.0
23	1806.0
24	3046.0
25	5018.0
26	7343.0
27	10929.0
28	15630.0
29	21912.0
30	30079.0
31	40633.0
32	53420.0
33	71632.0
34	100939.0
35	143855.0
36	217839.0
37	376485.0
38	897357.0
39	1697850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12028208506688	16.993120958197842	12.60417545613272	27.28242150060255
2	17.88526528501669	19.34131470940132	36.76541699390525	26.00800301167674
3	19.926936549250218	21.35691506509073	29.4300531586907	29.286095226968357
4	15.098166165802324	14.670484269830657	33.73786512794217	36.49348443642484
5	16.058760149804954	34.976882352050296	33.475371104779086	15.488986393365655
6	36.45243086882114	33.45349209543034	14.898739942146383	15.195337093602134
7	30.56027031529573	29.745500334270154	20.370196083522103	19.324033266912014
8	27.200557765993583	35.291058435615675	19.034197243190736	18.474186555200014
9	27.947770558696323	14.5512179765946	17.843724916216104	39.65728654849297
10	17.037257600048463	25.985961735262364	32.485460870919795	24.491319793769378
11	37.59535894556697	20.85875517361494	20.490246667575363	21.055639213242724
12	22.32251228361687	23.384171604994364	29.575498538514626	24.71781757287414
13	31.80786064937116	18.906952819228	23.50424923355856	25.780937297842282
14	23.10353071499196	19.07370927367098	24.77352926540618	33.049230745930885
15	26.589162507220887	25.998050631651598	21.566212535239	25.846574325888522
16	25.376540725788132	25.043460529077176	23.775667945331143	25.804330799803548
17	25.1003080910686	24.947587737802383	24.440313846140533	25.511790324988482
18	25.299815448257363	24.806469479728516	24.87207946326382	25.0216356087503
19	26.564281557158033	24.588003920372305	24.53102113582619	24.316693386643472
20	25.73874786077919	24.008007338798485	24.580377368287824	25.672867432134506
21	25.254921560100474	24.711083489650605	25.00684226126728	25.027152688981637
22	26.03572363538807	24.054145274458516	23.841548373975822	26.068582716177595
23	25.328239229253075	24.14590730007075	24.72495732376174	25.800896146914436
24	25.14019874470198	23.91973405509724	24.77488149095307	26.16518570924771
25	25.79575768983624	23.729557054182056	24.433255228785743	26.04143002719596
26	25.366777657339558	23.99199698832326	24.975200183469962	25.66602517086722
27	26.019929641000346	24.47812207243169	24.473578594594127	25.02836969197384
28	25.512223037163483	24.922896099316098	23.93788092193656	25.626999941583854
29	25.391063628161774	24.899691908931395	24.585461736344143	25.123782726562688
30	26.10647207600157	24.3763265332615	24.260873516067686	25.256327874669243
31	25.66099489183277	23.959083818511857	24.595143671259905	25.78477761839546
32	24.93038742884589	24.445398214196853	24.640145737460543	25.984068619496714
33	25.216815844189	23.84606480730245	24.770824814312387	26.16629453419616
34	25.529423346119977	23.911242078662745	25.361476933195732	25.197857642021543
35	25.34232941945171	23.868728107468396	25.065555894513423	25.72338657856647
36	25.066340185330628	23.453270330440652	25.614261976932113	25.86612750729661
37	24.6026350007897	23.22588008247494	25.91196795333632	26.259516963399044
38	24.958703031797853	22.827920104024006	25.969978429298074	26.24339843488006
39	24.08421877062144	22.85637092953066	27.02563386924736	26.03377643060054
40	24.903559273995512	22.500762655208447	27.119397188668998	25.47628088212704
41	24.048195482052183	22.689533341554867	27.390275010222826	25.87199616617013
42	23.540110255062192	22.931203091295778	27.732171717499533	25.7965149361425
43	23.17920125659616	22.612131951250646	27.40133621519642	26.80733057695678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77.0
1	111.5
2	146.0
3	439.0
4	732.0
5	732.0
6	1002.0
7	1272.0
8	1138.5
9	1005.0
10	1327.5
11	1650.0
12	1650.0
13	2970.5
14	4291.0
15	6707.0
16	9123.0
17	8621.0
18	8119.0
19	8119.0
20	9507.5
21	10896.0
22	11408.5
23	11921.0
24	15481.5
25	19042.0
26	19042.0
27	24495.0
28	29948.0
29	35939.5
30	41931.0
31	50574.5
32	59218.0
33	59218.0
34	71004.0
35	82790.0
36	96435.5
37	110081.0
38	127928.0
39	145775.0
40	145775.0
41	165454.0
42	185133.0
43	207575.5
44	230018.0
45	257816.5
46	285615.0
47	285615.0
48	311903.5
49	338192.0
50	354208.5
51	370225.0
52	374070.0
53	377915.0
54	377915.0
55	355086.0
56	332257.0
57	307970.0
58	283683.0
59	260969.0
60	238255.0
61	238255.0
62	208334.0
63	178413.0
64	149958.5
65	121504.0
66	101209.5
67	80915.0
68	80915.0
69	66423.5
70	51932.0
71	42687.5
72	33443.0
73	27392.5
74	21342.0
75	21342.0
76	17356.5
77	13371.0
78	10740.5
79	8110.0
80	6418.5
81	4727.0
82	4727.0
83	3626.0
84	2525.0
85	1871.0
86	1217.0
87	892.5
88	568.0
89	568.0
90	358.0
91	148.0
92	99.0
93	50.0
94	34.0
95	18.0
96	18.0
97	14.0
98	10.0
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3697608.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.71517055818841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82389563729102	46.647649218834715
2	10.06271682595549	11.61542835777547
3	3.3248117259082326	5.756762275039752
4	1.6162826144423337	3.7313610705109586
5	0.9524264827751735	2.7484728448752316
6	0.6455414920645186	2.2354522390134672
7	0.4322127376932849	1.7461662311372639
8	0.3362336159704863	1.552462439450643
9	0.25514994514030015	1.3253420341516353
>10	1.4434817063092096	15.02058270798568
>50	0.07138646072063601	2.780887622484868
>100	0.03351532810165004	3.4566886384352116
>500	0.0017356164578088932	0.6753718178385643
>1k	6.098111699158468E-4	0.7073725024665822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4316	0.11672410920789873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.7044510937882004E-5	0.0	2.7044510937882004E-5	0.0
6	0.0	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.0
7	0.0	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.0
8	0.0	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5
9	0.0	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5
10	0.0	2.7044510937882004E-5	2.7044510937882004E-5	5.408902187576401E-5	2.7044510937882004E-5
11	0.0	2.7044510937882004E-5	2.7044510937882004E-5	8.113353281364601E-5	2.7044510937882004E-5
12	0.0	2.7044510937882004E-5	2.7044510937882004E-5	8.113353281364601E-5	8.113353281364601E-5
13	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	8.113353281364601E-5	1.0817804375152801E-4
14	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	1.3522255468941002E-4	1.8931157656517403E-4
15	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	1.3522255468941002E-4	2.1635608750305603E-4
16	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	1.3522255468941002E-4	2.7044510937882004E-4
17	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	1.6226706562729202E-4	2.7044510937882004E-4
18	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	1.8931157656517403E-4	2.9748962031670204E-4
19	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	3.5157864219246605E-4	2.9748962031670204E-4
20	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	4.3271217500611206E-4	3.5157864219246605E-4
21	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	7.302017953228141E-4	3.7862315313034805E-4
22	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.0012170029922046902	4.0566766406823006E-4
23	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.0015685816343971562	4.3271217500611206E-4
24	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.002406961473471498	4.5975668594399406E-4
25	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.0034076083781731325	4.8680119688187607E-4
26	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.0052195906110112265	4.8680119688187607E-4
27	2.7044510937882004E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.015334237701779095	4.8680119688187607E-4
28	5.408902187576401E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.05655007237111127	4.8680119688187607E-4
29	5.408902187576401E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.12567584232833767	4.8680119688187607E-4
30	5.408902187576401E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.2261732449735072	4.8680119688187607E-4
31	5.408902187576401E-5	2.7044510937882004E-5	2.7044510937882004E-5	0.6841179486846631	4.8680119688187607E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3085	0.0	21.34846	37
TACTTAC	985	0.0	16.340101	31
TCTTATA	5220	0.0	16.160921	37
ACGTATA	185	1.8189894E-11	16.0	29
TAATACG	310	0.0	15.5161295	27
CGCGGTA	320	0.0	14.453126	22
AGTGTAC	1235	0.0	14.380567	27
CTCGTTA	1030	0.0	14.368933	22
TCGTTAC	1010	0.0	14.10396	23
TAGATCG	120	3.30425E-5	13.875001	5
ACGGATG	1710	0.0	13.631579	28
GTACTTA	1215	0.0	13.55144	30
TACGGAT	1665	0.0	13.444445	27
GGGCGTC	1010	0.0	13.371286	11
ATACCGT	180	5.1688403E-8	13.361111	6
ACTTACA	965	0.0	13.227979	32
CGTTACG	815	0.0	12.93865	24
GTTACGG	1330	0.0	12.9360895	25
GGAGTGT	1640	0.0	12.8597555	6
AGGGCGT	1080	0.0	12.847222	10
>>END_MODULE