##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211942.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1702780
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.418938441842165	34.0	31.0	34.0	31.0	34.0
2	32.559109221390905	34.0	31.0	34.0	31.0	34.0
3	32.63561293884119	34.0	31.0	34.0	31.0	34.0
4	36.13438024876966	37.0	35.0	37.0	35.0	37.0
5	36.01476350438694	37.0	35.0	37.0	35.0	37.0
6	36.033544556548705	37.0	35.0	37.0	35.0	37.0
7	36.018774004862635	37.0	35.0	37.0	35.0	37.0
8	35.994553612328076	37.0	35.0	37.0	35.0	37.0
9	37.83675401402412	39.0	38.0	39.0	35.0	39.0
10	37.69872796250837	39.0	37.0	39.0	35.0	39.0
11	37.80428123421699	39.0	38.0	39.0	35.0	39.0
12	37.72765418903205	39.0	37.0	39.0	35.0	39.0
13	37.7863493815995	39.0	38.0	39.0	35.0	39.0
14	39.149392170450675	40.0	39.0	41.0	36.0	41.0
15	39.11275267503729	40.0	38.0	41.0	36.0	41.0
16	39.0676417388036	40.0	38.0	41.0	36.0	41.0
17	39.04995654165541	40.0	38.0	41.0	36.0	41.0
18	39.035423836314735	40.0	38.0	41.0	36.0	41.0
19	39.04328392393615	40.0	39.0	41.0	36.0	41.0
20	39.005478100517976	40.0	39.0	41.0	36.0	41.0
21	38.99317645262453	40.0	38.0	41.0	36.0	41.0
22	38.96112298711519	40.0	38.0	41.0	36.0	41.0
23	38.89932169745945	40.0	38.0	41.0	35.0	41.0
24	38.86896604376373	40.0	38.0	41.0	35.0	41.0
25	38.813866148298665	40.0	38.0	41.0	35.0	41.0
26	38.71755599666428	40.0	38.0	41.0	35.0	41.0
27	38.637790554270076	40.0	38.0	41.0	35.0	41.0
28	38.577076310504	40.0	38.0	41.0	35.0	41.0
29	38.52064917370418	40.0	38.0	41.0	34.0	41.0
30	38.46641903240583	40.0	38.0	41.0	34.0	41.0
31	38.431881981230696	40.0	38.0	41.0	34.0	41.0
32	38.38317927154418	40.0	38.0	41.0	34.0	41.0
33	38.348582905601425	40.0	38.0	41.0	34.0	41.0
34	38.29287518058704	40.0	38.0	41.0	34.0	41.0
35	38.2512232936727	40.0	38.0	41.0	34.0	41.0
36	38.19761390197207	40.0	38.0	41.0	34.0	41.0
37	38.17711213427454	40.0	38.0	41.0	34.0	41.0
38	38.128475199379835	40.0	38.0	41.0	33.0	41.0
39	38.09359635419725	40.0	38.0	41.0	33.0	41.0
40	38.05659685925369	40.0	38.0	41.0	33.0	41.0
41	37.98289033228016	40.0	37.0	41.0	33.0	41.0
42	37.90790706961557	40.0	37.0	41.0	33.0	41.0
43	36.96433244459061	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	2.0
15	1.0
16	10.0
17	17.0
18	20.0
19	62.0
20	147.0
21	309.0
22	553.0
23	982.0
24	1753.0
25	2807.0
26	4203.0
27	5950.0
28	8422.0
29	11760.0
30	15947.0
31	21733.0
32	28768.0
33	37931.0
34	52457.0
35	75572.0
36	114397.0
37	196148.0
38	463009.0
39	659818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92615605069357	16.735632318913776	12.475011451861075	27.863200178531578
2	18.808301718366437	18.806422438600407	35.3268184968111	27.05845734622206
3	20.365754824463526	20.445976579475918	28.78510435875451	30.40316423730605
4	15.7591702979833	14.370969825814257	32.5190570713774	37.35080280482505
5	16.80915913975969	34.03657548244635	33.18590775085449	15.968357626939476
6	38.09341194987021	32.2866136553166	14.290630615816488	15.329343778996698
7	31.63321157166516	28.622546658992942	19.67817334006742	20.06606842927448
8	27.896968486827422	34.30642831134968	18.477548479545213	19.319054722277688
9	28.025405513337017	13.95829173469268	17.197347866430192	40.818954885540116
10	17.4134650395236	25.440514922655893	32.09216692702522	25.053853110795288
11	38.83161653296374	19.916430777904367	19.762271109597247	21.489681579534643
12	22.96432892094105	22.835245892011887	28.875485970001996	25.324939217045067
13	32.77528512197701	18.15401872232467	22.634985141944348	26.435711013753977
14	23.782931441525037	18.306416565851137	23.6983638520537	34.212288140570124
15	27.18472145550218	25.20495894948261	20.722641797531097	26.887677797484116
16	26.497785973525644	24.139524777129164	22.80787888041908	26.554810368926105
17	26.079058950657156	24.006918098638696	23.557828962050294	26.356193988653846
18	26.32019403563584	23.812060277898496	23.85845499712235	26.009290689343313
19	27.393262782038786	23.63599525481859	23.56575717356323	25.404984789579395
20	26.81650007634574	23.23418174984437	23.487767063273	26.461551110536885
21	26.424728972621242	23.77981888441255	23.67099684046089	26.124455302505318
22	27.126111417799127	23.207930560612645	22.969262030326878	26.69669599126135
23	26.50277781040416	23.275173539740894	23.484771961145892	26.73727668870905
24	26.301577420453608	23.115082394672243	23.622487931500253	26.960852253373897
25	26.892023631943058	22.857797249204243	23.365320241017628	26.88485887783507
26	26.36259528535689	23.177685901878107	23.84024947438894	26.619469338376067
27	26.924969755341266	23.493052537614957	23.35304619504575	26.228931511998027
28	26.51939769083499	23.909254278297844	23.07485406218067	26.4964939686865
29	26.50213180798459	23.8424811191111	23.492523990180764	26.162863082723547
30	26.893080726811448	23.51243261020214	23.313698774944502	26.280787888041907
31	26.42102914058187	23.14380013859688	23.56064788169934	26.874522839121905
32	25.898883003089068	23.43696778209751	23.6547293249862	27.009419889827225
33	26.097088290912506	22.9818297137622	23.9049084438389	27.016173551486393
34	26.498079610989087	22.91288363734599	24.460999072105615	26.128037679559306
35	26.216246373577327	22.773993117137856	24.317527807467787	26.69223270181703
36	25.875274551028323	22.35350426948872	24.926003359212583	26.845217820270385
37	25.40328169229143	21.84445436286543	25.324763034567006	27.427500910276137
38	25.336684715582752	21.532611376689882	25.83522240101481	27.295481506712555
39	24.80508345176711	21.37968498572922	26.743971622875534	27.07125993962814
40	25.131549583622075	21.203208870200495	27.059044621148942	26.60619692502848
41	24.34507100153866	21.23175043164707	27.577490926602376	26.845687640211892
42	23.543616908819693	21.531025734387296	27.908537802887047	27.016819553905965
43	23.21632859206709	21.144129012555936	27.900609591374103	27.738932804002864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	17.5
2	19.0
3	40.5
4	62.0
5	62.0
6	91.5
7	121.0
8	109.5
9	98.0
10	129.5
11	161.0
12	161.0
13	294.0
14	427.0
15	658.5
16	890.0
17	914.0
18	938.0
19	938.0
20	1266.0
21	1594.0
22	1941.0
23	2288.0
24	3233.5
25	4179.0
26	4179.0
27	5609.5
28	7040.0
29	9115.5
30	11191.0
31	14325.5
32	17460.0
33	17460.0
34	22251.0
35	27042.0
36	33078.0
37	39114.0
38	47313.5
39	55513.0
40	55513.0
41	65251.5
42	74990.0
43	86605.0
44	98220.0
45	112160.0
46	126100.0
47	126100.0
48	140198.0
49	154296.0
50	164809.5
51	175323.0
52	177031.5
53	178740.0
54	178740.0
55	173546.0
56	168352.0
57	158305.5
58	148259.0
59	137824.0
60	127389.0
61	127389.0
62	112094.5
63	96800.0
64	81732.0
65	66664.0
66	55691.5
67	44719.0
68	44719.0
69	36771.5
70	28824.0
71	23530.5
72	18237.0
73	15022.0
74	11807.0
75	11807.0
76	9470.0
77	7133.0
78	5719.0
79	4305.0
80	3330.5
81	2356.0
82	2356.0
83	1782.0
84	1208.0
85	887.0
86	566.0
87	396.5
88	227.0
89	227.0
90	150.0
91	73.0
92	51.0
93	29.0
94	17.5
95	6.0
96	6.0
97	4.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1702780.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.88010196050188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.37747223854143	58.96306363198691
2	8.854302615341158	12.374791390983573
3	2.741935920804334	5.748202851449084
4	1.3207263161387421	3.6916995853477332
5	0.7494793301804582	2.6186846005149533
6	0.47148161653065507	1.9768310061398655
7	0.3155274974700484	1.5434365586184284
8	0.23155133288704668	1.2944664600989548
9	0.16107112587820177	1.0130100029335414
>10	0.7428927097366581	8.575245831543231
>50	0.02488997702533011	1.1658563235562507
>100	0.00858517697647287	0.9926705738096984
>500	8.414248937519305E-5	0.0420411830177549
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7618247806528147E-4
2	0.0	0.0	0.0	5.872749268842716E-5	1.7618247806528147E-4
3	0.0	0.0	0.0	5.872749268842716E-5	1.7618247806528147E-4
4	0.0	0.0	0.0	5.872749268842716E-5	2.3490997075370864E-4
5	0.0	0.0	0.0	5.872749268842716E-5	2.3490997075370864E-4
6	0.0	0.0	0.0	5.872749268842716E-5	2.3490997075370864E-4
7	0.0	0.0	0.0	5.872749268842716E-5	2.3490997075370864E-4
8	0.0	0.0	0.0	5.872749268842716E-5	2.3490997075370864E-4
9	0.0	0.0	0.0	1.1745498537685432E-4	2.3490997075370864E-4
10	0.0	0.0	0.0	1.1745498537685432E-4	2.3490997075370864E-4
11	0.0	0.0	0.0	1.1745498537685432E-4	2.3490997075370864E-4
12	0.0	0.0	0.0	1.1745498537685432E-4	2.936374634421358E-4
13	0.0	0.0	0.0	1.1745498537685432E-4	3.5236495613056294E-4
14	0.0	0.0	0.0	1.1745498537685432E-4	7.047299122611259E-4
15	0.0	0.0	0.0	1.1745498537685432E-4	8.221848976379802E-4
16	0.0	0.0	0.0	1.1745498537685432E-4	9.983673757032617E-4
17	0.0	0.0	0.0	1.1745498537685432E-4	9.983673757032617E-4
18	0.0	0.0	0.0	1.1745498537685432E-4	9.983673757032617E-4
19	0.0	0.0	0.0	1.1745498537685432E-4	9.983673757032617E-4
20	0.0	0.0	0.0	1.1745498537685432E-4	9.983673757032617E-4
21	0.0	0.0	0.0	2.936374634421358E-4	9.983673757032617E-4
22	0.0	0.0	0.0	5.872749268842716E-4	9.983673757032617E-4
23	0.0	0.0	0.0	8.221848976379802E-4	0.0012332773464569704
24	0.0	0.0	0.0	0.0019380072587180963	0.0012920048391453975
25	0.0	0.0	0.0	0.0028189196490445035	0.0012920048391453975
26	0.0	0.0	0.0	0.005226746849270017	0.0013507323318338246
27	0.0	0.0	0.0	0.020965714889768495	0.0014094598245222517
28	0.0	0.0	0.0	0.08004557253432622	0.0014094598245222517
29	0.0	0.0	0.0	0.1673733541620174	0.0014094598245222517
30	0.0	0.0	0.0	0.29457710332515064	0.0014094598245222517
31	0.0	0.0	0.0	0.8353985834928763	0.0014094598245222517
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1810	0.0	20.74862	37
TATTCCG	45	0.003826554	20.555555	5
CGACTTA	50	0.007036238	18.5	14
TTAGCCG	105	4.800622E-7	17.61905	4
GTATATG	105	4.800622E-7	17.61905	1
GTCTAGA	245	0.0	16.612246	1
CTCGTTA	285	0.0	16.22807	22
TTGTATA	115	1.2429773E-6	16.086956	2
CTCGTAT	105	9.3482995E-6	15.857144	29
GGCTAAC	135	3.9751103E-7	15.074075	1
TCTTATA	2965	0.0	14.974705	37
TCGTAAG	75	0.004105486	14.799999	2
TACCGTC	90	8.2769006E-4	14.388888	7
GCTATAT	105	1.6563693E-4	14.095239	1
GTACCGT	120	3.302192E-5	13.875001	6
ATCTATA	120	3.302192E-5	13.875001	2
GAGTATA	80	0.006300265	13.875	1
CGATCGT	80	0.006300265	13.875	25
ATTATAC	135	6.570939E-6	13.703704	3
CGGTCGT	95	0.0012455046	13.631579	17
>>END_MODULE