##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211940.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4798377
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.610501008986994	34.0	31.0	34.0	31.0	34.0
2	32.735935921666844	34.0	31.0	34.0	31.0	34.0
3	32.81618618128588	34.0	31.0	34.0	31.0	34.0
4	36.262386010936616	37.0	37.0	37.0	35.0	37.0
5	36.17100177830962	37.0	35.0	37.0	35.0	37.0
6	36.18951241221772	37.0	37.0	37.0	35.0	37.0
7	36.17511483570382	37.0	36.0	37.0	35.0	37.0
8	36.15882745353272	37.0	36.0	37.0	35.0	37.0
9	38.00959991263713	39.0	38.0	39.0	35.0	39.0
10	37.898060948524886	39.0	38.0	39.0	35.0	39.0
11	37.98904900552833	39.0	38.0	39.0	35.0	39.0
12	37.92987045411397	39.0	38.0	39.0	35.0	39.0
13	37.978219927279575	39.0	38.0	39.0	35.0	39.0
14	39.401081657402074	40.0	39.0	41.0	37.0	41.0
15	39.373418762218975	40.0	39.0	41.0	36.0	41.0
16	39.344772826311896	40.0	39.0	41.0	36.0	41.0
17	39.32529790802182	40.0	39.0	41.0	36.0	41.0
18	39.31890428784566	40.0	39.0	41.0	36.0	41.0
19	39.3288013843014	40.0	39.0	41.0	36.0	41.0
20	39.29211689702581	40.0	39.0	41.0	36.0	41.0
21	39.28189156458527	40.0	39.0	41.0	36.0	41.0
22	39.25715799321312	40.0	39.0	41.0	36.0	41.0
23	39.204655865931336	40.0	39.0	41.0	36.0	41.0
24	39.18038078291889	40.0	39.0	41.0	36.0	41.0
25	39.13710073218507	40.0	39.0	41.0	36.0	41.0
26	39.05644929525129	40.0	39.0	41.0	36.0	41.0
27	38.98512371995781	40.0	39.0	41.0	36.0	41.0
28	38.933678616748956	40.0	39.0	41.0	35.0	41.0
29	38.884057046788946	40.0	38.0	41.0	35.0	41.0
30	38.83308335297539	40.0	38.0	41.0	35.0	41.0
31	38.79776787026113	40.0	38.0	41.0	35.0	41.0
32	38.747722406972194	40.0	38.0	41.0	35.0	41.0
33	38.71548213072879	40.0	38.0	41.0	35.0	41.0
34	38.67210413020903	40.0	38.0	41.0	35.0	41.0
35	38.62553213305249	40.0	38.0	41.0	35.0	41.0
36	38.5761975351249	40.0	38.0	41.0	35.0	41.0
37	38.55029815289628	40.0	38.0	41.0	35.0	41.0
38	38.50361591012961	40.0	38.0	41.0	35.0	41.0
39	38.47008436394223	40.0	38.0	41.0	34.0	41.0
40	38.424769666910294	40.0	38.0	41.0	34.0	41.0
41	38.35905161265986	40.0	38.0	41.0	34.0	41.0
42	38.27905623088807	40.0	38.0	41.0	34.0	41.0
43	37.3986670909768	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	0.0
13	1.0
14	1.0
15	7.0
16	9.0
17	21.0
18	57.0
19	112.0
20	280.0
21	589.0
22	1142.0
23	2035.0
24	3737.0
25	5959.0
26	9219.0
27	13583.0
28	19159.0
29	26856.0
30	36892.0
31	50188.0
32	66901.0
33	89655.0
34	124973.0
35	178960.0
36	269940.0
37	465002.0
38	1141842.0
39	2291252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85786631604812	17.310269701609524	12.561914163893334	27.269949818449028
2	17.721367037229463	19.25834506125717	36.74044369585799	26.279844205655372
3	19.64870621879023	21.388356938189727	29.30086568854427	29.662071154475772
4	14.75252986582755	14.672898773897924	34.02700538119451	36.54756597908001
5	15.825517669828779	35.00504441397581	33.8781217065687	15.291316209626713
6	36.5486913595993	33.86311663297819	14.835620460834987	14.752571546587523
7	30.771175336994155	29.612825336566928	20.428386514857003	19.187612811581918
8	27.26882443792974	35.2783659975029	18.904954737820727	18.547854826746626
9	27.510822930336655	14.618130255292572	17.965720492574885	39.90532632179589
10	16.896025468611576	26.173912554182383	32.7562423711184	24.17381960608764
11	37.55794928160084	20.796073338964405	20.63139265630858	21.014584723126173
12	22.277907717546995	23.70695341362298	29.77044113040722	24.244697738422804
13	31.624713939734207	19.00761444963578	23.50769854056903	25.85997307006098
14	22.98022852310271	18.992025845405642	24.84521328774292	33.182532343748726
15	26.021298451538925	26.27407142039902	21.5923217371207	26.11230839094135
16	25.574939192981294	25.1441685386538	23.494235655097548	25.78665661326736
17	25.01945970481269	25.129476070763097	24.59698352171995	25.254080702704268
18	25.38854283437921	24.7062913147508	24.759726049037	25.145439801832996
19	26.296058021285113	24.536588100518156	24.64391605745026	24.523437820746473
20	25.761731518803128	24.337020621764403	24.507453249296585	25.393794610135885
21	25.37545507574749	24.66734064455544	24.738510542210417	25.218693737486653
22	25.92964246035691	24.312303931100036	24.154167127760072	25.603886480782982
23	25.46800720326894	24.274624524083873	24.425884002028187	25.831484270619004
24	25.33358675235397	24.27549982004332	24.52495916848551	25.865954259117196
25	25.682767319033083	24.02220584168355	24.38710005487272	25.907926784410645
26	25.401317987311128	24.313866959599046	24.728507159816747	25.55630789327308
27	25.842696395051913	24.464792991463572	24.394727633947895	25.29778297953662
28	25.530507502849403	24.733092043413844	24.212145064883398	25.524255388853355
29	25.517503105737628	24.755870578739437	24.413442295176058	25.31318402034688
30	25.665469803644026	24.590877290383812	24.375012634480367	25.3686402714918
31	25.51650276749826	24.151478718741775	24.51351779987275	25.818500713887214
32	24.95045720667634	24.503139290639314	24.66642366783602	25.879979834848328
33	25.181243574650345	24.171131197069343	24.802553029909905	25.845072198370406
34	25.601156391004707	23.96599933685911	25.077896130295724	25.35494814184046
35	25.328230774697364	24.08270546478528	25.00301664500309	25.586047115514265
36	25.07376973505833	23.834517379522286	25.307848883070257	25.783864002349127
37	24.91825881959671	23.376737592731875	25.522213031614648	26.182790556056766
38	24.80357420852926	23.210598083476974	25.936582306892518	26.049245401101246
39	24.32551673201168	23.057358769433915	26.562231354476733	26.054893144077674
40	24.742657777827795	22.88530059226276	26.834010749884808	25.538030880024642
41	24.190741994636937	22.846516645107293	27.11062511344982	25.852116246805952
42	23.549754427382425	23.154224855612636	27.403828419484338	25.8921922975206
43	23.518598059302136	22.709574508213922	27.29777172573143	26.474055706752512
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	78.0
1	102.5
2	127.0
3	373.5
4	620.0
5	620.0
6	785.0
7	950.0
8	923.5
9	897.0
10	1232.5
11	1568.0
12	1568.0
13	2698.5
14	3829.0
15	5939.5
16	8050.0
17	8262.5
18	8475.0
19	8475.0
20	10484.0
21	12493.0
22	14203.5
23	15914.0
24	21328.5
25	26743.0
26	26743.0
27	33752.0
28	40761.0
29	49334.5
30	57908.0
31	70874.0
32	83840.0
33	83840.0
34	99280.5
35	114721.0
36	133121.5
37	151522.0
38	174843.0
39	198164.0
40	198164.0
41	223444.0
42	248724.0
43	276032.5
44	303341.0
45	335857.5
46	368374.0
47	368374.0
48	398397.5
49	428421.0
50	444968.5
51	461516.0
52	463555.5
53	465595.0
54	465595.0
55	450373.5
56	435152.0
57	403557.0
58	371962.0
59	342861.5
60	313761.0
61	313761.0
62	275599.5
63	237438.0
64	201037.0
65	164636.0
66	135203.5
67	105771.0
68	105771.0
69	85866.0
70	65961.0
71	53516.5
72	41072.0
73	33343.5
74	25615.0
75	25615.0
76	20314.5
77	15014.0
78	12169.5
79	9325.0
80	7256.5
81	5188.0
82	5188.0
83	3959.5
84	2731.0
85	1997.5
86	1264.0
87	933.0
88	602.0
89	602.0
90	386.0
91	170.0
92	114.0
93	58.0
94	35.5
95	13.0
96	13.0
97	11.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4798377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.773313585565866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.46639227254137	47.292765061332275
2	10.468568488597342	12.305449171446098
3	3.4298494901682623	6.047508589108575
4	1.6403927905294293	3.8564527952515046
5	0.9469684580842044	2.7828237071311364
6	0.6143181947154305	2.166330953959722
7	0.4218563753947499	1.735572792740208
8	0.30533254304384067	1.435632424015515
9	0.24827456560969097	1.3132727009908662
>10	1.3466456580748734	14.178459379324886
>50	0.08074729093187594	3.226479237302448
>100	0.029374991135591662	2.8870682153848626
>500	9.591614982037963E-4	0.35322952832041693
>1k	3.197196749725812E-4	0.41895544369152954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.0840379986816376E-5	0.0	0.0	0.0	4.168075997363275E-5
7	2.0840379986816376E-5	2.0840379986816376E-5	0.0	2.0840379986816376E-5	4.168075997363275E-5
8	2.0840379986816376E-5	2.0840379986816376E-5	0.0	2.0840379986816376E-5	6.252113996044912E-5
9	2.0840379986816376E-5	2.0840379986816376E-5	0.0	4.168075997363275E-5	8.33615199472655E-5
10	2.0840379986816376E-5	2.0840379986816376E-5	0.0	6.252113996044912E-5	8.33615199472655E-5
11	2.0840379986816376E-5	2.0840379986816376E-5	0.0	6.252113996044912E-5	8.33615199472655E-5
12	2.0840379986816376E-5	2.0840379986816376E-5	0.0	6.252113996044912E-5	1.2504227992089824E-4
13	2.0840379986816376E-5	2.0840379986816376E-5	0.0	6.252113996044912E-5	1.2504227992089824E-4
14	2.0840379986816376E-5	2.0840379986816376E-5	0.0	8.33615199472655E-5	1.4588265990771464E-4
15	2.0840379986816376E-5	2.0840379986816376E-5	0.0	8.33615199472655E-5	2.0840379986816375E-4
16	2.0840379986816376E-5	2.0840379986816376E-5	0.0	1.66723039894531E-4	2.500845598417965E-4
17	2.0840379986816376E-5	2.0840379986816376E-5	0.0	1.66723039894531E-4	2.500845598417965E-4
18	2.0840379986816376E-5	2.0840379986816376E-5	0.0	1.8756341988134738E-4	2.500845598417965E-4
19	2.0840379986816376E-5	2.0840379986816376E-5	0.0	2.0840379986816375E-4	2.500845598417965E-4
20	2.0840379986816376E-5	2.0840379986816376E-5	0.0	3.1260569980224564E-4	2.709249398286129E-4
21	2.0840379986816376E-5	2.0840379986816376E-5	0.0	5.00169119683593E-4	2.709249398286129E-4
22	2.0840379986816376E-5	2.0840379986816376E-5	0.0	6.877325395649404E-4	2.709249398286129E-4
23	2.0840379986816376E-5	2.0840379986816376E-5	0.0	8.961363394331042E-4	4.168075997363275E-4
24	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.0015213477390375954	4.168075997363275E-4
25	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.002000676478734372	4.168075997363275E-4
26	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.00310521661803564	4.376479797231439E-4
27	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.012045739632379865	4.376479797231439E-4
28	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.04880816992912395	4.376479797231439E-4
29	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.10534812083335678	4.376479797231439E-4
30	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.19096040181919846	4.376479797231439E-4
31	2.0840379986816376E-5	2.0840379986816376E-5	0.0	0.5754654125759606	4.584883597099603E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3315	0.0	20.313726	37
TCTTATA	5855	0.0	16.55679	37
TACTTAC	575	0.0	13.834783	31
TAGACAG	900	0.0	12.744445	5
GTAACGT	205	2.0703737E-8	12.634147	26
TACGGAT	905	0.0	12.469613	27
CTCTTAT	9010	0.0	12.381244	37
ATTACGC	135	9.9727964E-5	12.333334	3
TAACCCG	120	5.126703E-4	12.333333	5
TATACCG	165	3.8100625E-6	12.333333	5
CTCGTTA	510	0.0	11.970588	22
CGTTACG	310	3.6379788E-12	11.935483	24
GGTATCA	5310	0.0	11.636535	1
GTCTTAG	830	0.0	11.36747	1
ATTAGAC	345	1.8189894E-12	11.26087	3
GTATTAA	840	0.0	11.232142	1
TCGTTAC	430	0.0	11.186047	23
ACCGTTA	450	0.0	11.1	8
CGATCGT	350	3.6379788E-12	11.1	25
CTTAGAC	735	0.0	11.07483	3
>>END_MODULE