FastQCFastQC Report
Thu 9 Feb 2017
SRR3211939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895510
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40610.10424822423764796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC33600.021.52827337
TATACCG1800.017.4722235
TCTTATA59550.016.62048737
TACTTAC10300.015.26699131
GTAACGT4300.014.62790726
CGCGGTA3550.014.59154922
CTCGTTA11000.013.9590922
TAGTACT2950.013.796614
TCGTTAC10200.013.78431323
TCTATAC4600.013.6739133
CGGTAAT3800.013.63157924
CGTTACG9150.013.54644924
TAGACAG6150.013.2357725
TTAGCCG4900.013.2142854
TACGGAT17600.013.03409127
TTTATAC5050.012.8217813
GTACTTA12700.012.81889730
GGAGTGT19350.012.811376
AGTGTAC14550.012.58762927
GTGTACT14150.012.55123628
Produced by FastQC (version 0.11.5)