##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211939.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3895510
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62164851328838	34.0	31.0	34.0	31.0	34.0
2	32.7472893151346	34.0	31.0	34.0	31.0	34.0
3	32.823278338394715	34.0	31.0	34.0	31.0	34.0
4	36.264134606251815	37.0	37.0	37.0	35.0	37.0
5	36.17644262240374	37.0	35.0	37.0	35.0	37.0
6	36.19430934588796	37.0	37.0	37.0	35.0	37.0
7	36.17984551445125	37.0	36.0	37.0	35.0	37.0
8	36.162202381716384	37.0	36.0	37.0	35.0	37.0
9	38.014141665661235	39.0	38.0	39.0	35.0	39.0
10	37.90629750661659	39.0	38.0	39.0	35.0	39.0
11	37.99843435134296	39.0	38.0	39.0	35.0	39.0
12	37.93984998113213	39.0	38.0	39.0	35.0	39.0
13	37.987170357668184	39.0	38.0	39.0	35.0	39.0
14	39.400459503376965	40.0	39.0	41.0	37.0	41.0
15	39.37510005108445	40.0	39.0	41.0	36.0	41.0
16	39.33739818406319	40.0	39.0	41.0	36.0	41.0
17	39.32384745514708	40.0	39.0	41.0	36.0	41.0
18	39.31421688045981	40.0	39.0	41.0	36.0	41.0
19	39.32713791005542	40.0	39.0	41.0	36.0	41.0
20	39.29369581903268	40.0	39.0	41.0	36.0	41.0
21	39.28151795271992	40.0	39.0	41.0	36.0	41.0
22	39.25413514533399	40.0	39.0	41.0	36.0	41.0
23	39.200365292349396	40.0	39.0	41.0	36.0	41.0
24	39.17515601294824	40.0	39.0	41.0	36.0	41.0
25	39.12662988928279	40.0	39.0	41.0	36.0	41.0
26	39.042461449206904	40.0	39.0	41.0	36.0	41.0
27	38.964125878254706	40.0	39.0	41.0	35.0	41.0
28	38.908363218166556	40.0	38.0	41.0	35.0	41.0
29	38.8542160076601	40.0	38.0	41.0	35.0	41.0
30	38.8046997697349	40.0	38.0	41.0	35.0	41.0
31	38.76477483051	40.0	38.0	41.0	35.0	41.0
32	38.70503194703646	40.0	38.0	41.0	35.0	41.0
33	38.67567122148319	40.0	38.0	41.0	35.0	41.0
34	38.62683217345097	40.0	38.0	41.0	35.0	41.0
35	38.581142392138645	40.0	38.0	41.0	35.0	41.0
36	38.52046304591697	40.0	38.0	41.0	35.0	41.0
37	38.49932922775195	40.0	38.0	41.0	35.0	41.0
38	38.454455000757285	40.0	38.0	41.0	34.0	41.0
39	38.407647008992406	40.0	38.0	41.0	34.0	41.0
40	38.36469191453751	40.0	38.0	41.0	34.0	41.0
41	38.29043385846783	40.0	38.0	41.0	34.0	41.0
42	38.20878318885075	40.0	38.0	41.0	34.0	41.0
43	37.31776455457694	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.0
17	19.0
18	48.0
19	89.0
20	249.0
21	470.0
22	943.0
23	1837.0
24	3111.0
25	4937.0
26	7563.0
27	11182.0
28	15898.0
29	22524.0
30	30848.0
31	40965.0
32	54833.0
33	73653.0
34	103051.0
35	147883.0
36	222223.0
37	383032.0
38	921132.0
39	1849015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72678031887994	17.314729008525198	12.751655110627363	27.206835561967495
2	17.934724849891285	19.321577919194148	36.71775967716679	26.025937553747774
3	19.76092989107973	21.279883763615036	29.63183254567438	29.327353799630856
4	15.13224199142089	14.739482121724755	33.72046278921117	36.407813097643185
5	16.05094069839379	34.9645104235389	33.5125567640694	15.47199211399791
6	36.39007472705756	33.55599138495345	14.814799602619427	15.239134285369566
7	30.487227603060962	29.962058883175757	20.22518232529245	19.32553118847083
8	27.188455426888908	35.2729424388591	18.97274041139671	18.56586172285529
9	27.88063693842398	14.573521823843347	17.923147418438152	39.62269381929452
10	17.182961922829104	26.070398997820565	32.309479374972724	24.437159704377603
11	37.53567568816407	20.867614253332682	20.43835081927655	21.158359239226698
12	22.253466170026517	23.540024284368414	29.466257306488753	24.740252239116316
13	31.91148270701397	18.957851475159863	23.258418024854254	25.87224779297191
14	23.009849801438065	19.224106728002237	24.691914537506	33.07412893305369
15	26.66454456540992	25.967870702424072	21.447435637439	25.92014909472701
16	25.332857571922546	25.120741571706912	23.73273332631671	25.81366753005383
17	25.117122019966576	24.879130075394492	24.412387594949056	25.591360309689875
18	25.32944338481996	24.89440407032712	24.690810702578098	25.085341842274822
19	26.531339927249576	24.625119689077938	24.574009564858006	24.26953081881448
20	25.88372254210617	24.045375316710775	24.354269402465917	25.716632738717138
21	25.287215281182696	24.861982128142397	24.844397781035088	25.006404809639815
22	26.172490893361843	24.15265266935523	23.694869221231624	25.9799872160513
23	25.38147764990977	24.271481782873103	24.479413478594587	25.86762708862254
24	25.24968489363395	23.967259742626766	24.602657931824073	26.180397431915203
25	25.785686598160446	23.724570081966164	24.23274487807758	26.256998441795808
26	25.32430926887622	24.17005732240451	24.840880911613628	25.664752497105642
27	26.172182846405224	24.32100033115048	24.343385076665186	25.163431745779114
28	25.434333373550576	25.13154888576849	23.76528362140002	25.668834119280916
29	25.518584216187357	24.901668844387512	24.361611188265464	25.21813575115967
30	26.03202148114111	24.41213088915187	24.17860562545084	25.377242004256185
31	25.588459534181663	24.147364529933178	24.45818390916722	25.805992026717938
32	24.917789968450858	24.441344008871752	24.54225505774597	26.098610964931424
33	25.163046687083334	23.90408444593904	24.697022982870024	26.2358458841076
34	25.63612980071929	23.940819045516506	25.231484452613394	25.191566701150812
35	25.362122032801864	23.831282681856806	25.000705940942265	25.805889344399063
36	25.038801081244817	23.45228224288989	25.67268470623872	25.83623196962657
37	24.591722264863908	23.197168021645435	25.880436707902177	26.330673005588483
38	24.83348778465464	22.729219023953217	26.109161573195806	26.32813161819633
39	24.026225064240624	22.748112570626184	27.17490136079743	26.050761004335758
40	24.794288809424184	22.433237239796586	27.16409404673586	25.60837990404337
41	23.922772627974258	22.616935908263617	27.55993438599824	25.900357077763886
42	23.227587658612094	22.89466591024025	27.915343562203667	25.96240286894399
43	22.983640139545273	22.511198790402283	27.630400127326077	26.874760942726372
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	104.0
2	116.0
3	363.0
4	610.0
5	610.0
6	808.5
7	1007.0
8	973.0
9	939.0
10	1277.0
11	1615.0
12	1615.0
13	2849.0
14	4083.0
15	6495.0
16	8907.0
17	8539.0
18	8171.0
19	8171.0
20	9646.0
21	11121.0
22	11727.5
23	12334.0
24	16061.0
25	19788.0
26	19788.0
27	25066.5
28	30345.0
29	36577.0
30	42809.0
31	52438.0
32	62067.0
33	62067.0
34	74029.0
35	85991.0
36	100515.5
37	115040.0
38	133752.5
39	152465.0
40	152465.0
41	173665.0
42	194865.0
43	219085.5
44	243306.0
45	272363.5
46	301421.0
47	301421.0
48	330999.5
49	360578.0
50	377987.5
51	395397.0
52	397098.5
53	398800.0
54	398800.0
55	376786.5
56	354773.0
57	328447.5
58	302122.0
59	276032.5
60	249943.0
61	249943.0
62	218256.5
63	186570.0
64	156658.0
65	126746.0
66	105124.0
67	83502.0
68	83502.0
69	68388.5
70	53275.0
71	43490.5
72	33706.0
73	27730.0
74	21754.0
75	21754.0
76	17703.5
77	13653.0
78	10993.0
79	8333.0
80	6563.5
81	4794.0
82	4794.0
83	3646.0
84	2498.0
85	1844.5
86	1191.0
87	862.5
88	534.0
89	534.0
90	362.5
91	191.0
92	117.0
93	43.0
94	25.5
95	8.0
96	8.0
97	6.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3895510.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.36461424679903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.46715768589424	46.96434617873567
2	10.49190585511238	12.247120758947323
3	3.399693387732096	5.952653793971314
4	1.602031627942556	3.7400783190415496
5	0.9496160808830354	2.7711988121647733
6	0.622467623682487	2.179804964241001
7	0.4450835966657025	1.8183992698880134
8	0.32269950705743394	1.506738579763147
9	0.24299063642025287	1.276384928422703
>10	1.353679415920645	14.132824551132565
>50	0.06750711837664351	2.671041726414039
>100	0.03274775755048908	3.4669943161372494
>500	0.0018037814510076286	0.6611139257411495
>1k	6.159253111012892E-4	0.611299875399456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4061	0.10424822423764796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.701173915610537E-5
2	0.0	0.0	0.0	0.0	1.0268231887480715E-4
3	0.0	0.0	0.0	0.0	1.0268231887480715E-4
4	0.0	0.0	0.0	0.0	1.0268231887480715E-4
5	0.0	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4
6	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.5402347831221073E-4
7	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.5402347831221073E-4
8	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	5.1341159437403576E-5	1.5402347831221073E-4
9	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4	2.5670579718701787E-4
10	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4	2.5670579718701787E-4
11	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4	2.5670579718701787E-4
12	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4	2.5670579718701787E-4
13	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4	2.5670579718701787E-4
14	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4	2.5670579718701787E-4
15	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.0268231887480715E-4	3.0804695662442147E-4
16	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	1.5402347831221073E-4	4.620704349366322E-4
17	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	2.053646377496143E-4	4.620704349366322E-4
18	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701787E-4	5.647527538114393E-4
19	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	3.337175363431232E-4	5.904233335301411E-4
20	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	5.390821740927375E-4	5.904233335301411E-4
21	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	8.727997104358608E-4	6.160939132488429E-4
22	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.0012321878264976859	6.160939132488429E-4
23	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.0016172465222782126	9.241408698732644E-4
24	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.002515716812432775	9.498114495919661E-4
25	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.003414187102587338	9.498114495919661E-4
26	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.005493504059802183	9.498114495919661E-4
27	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.019021899571558025	9.498114495919661E-4
28	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.06766764813849792	9.754820293106679E-4
29	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.1482732684552215	9.754820293106679E-4
30	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.2628153951600689	9.754820293106679E-4
31	2.5670579718701788E-5	2.5670579718701788E-5	2.5670579718701788E-5	0.7489134927134059	0.0010268231887480715
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3360	0.0	21.528273	37
TATACCG	180	0.0	17.472223	5
TCTTATA	5955	0.0	16.620487	37
TACTTAC	1030	0.0	15.266991	31
GTAACGT	430	0.0	14.627907	26
CGCGGTA	355	0.0	14.591549	22
CTCGTTA	1100	0.0	13.95909	22
TAGTACT	295	0.0	13.79661	4
TCGTTAC	1020	0.0	13.784313	23
TCTATAC	460	0.0	13.673913	3
CGGTAAT	380	0.0	13.631579	24
CGTTACG	915	0.0	13.546449	24
TAGACAG	615	0.0	13.235772	5
TTAGCCG	490	0.0	13.214285	4
TACGGAT	1760	0.0	13.034091	27
TTTATAC	505	0.0	12.821781	3
GTACTTA	1270	0.0	12.818897	30
GGAGTGT	1935	0.0	12.81137	6
AGTGTAC	1455	0.0	12.587629	27
GTGTACT	1415	0.0	12.551236	28
>>END_MODULE