FastQCFastQC Report
Thu 9 Feb 2017
SRR3211935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538597
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC26800.023.12537
GTAACGT1457.2759576E-1219.13793226
ATTACGC609.241009E-418.53
TACTTAC7200.017.47222131
GTATTAG1950.017.0769231
GGTATCA22800.015.903511
GTACTTA8400.015.41666830
TCTTATA46250.015.437
TAACGTC1954.1836756E-1115.17948727
GTATTAA3600.014.9027781
TATACAC7200.014.90277837
CGTTACG6650.014.46616624
TACGGAT13450.014.44237927
CTCGTTA9100.014.2307722
TAGACCG1051.6568751E-414.0952395
TAAGGCG1458.921397E-714.0344845
ACGGATG14400.013.87528
AGTGTAC10600.013.61320827
TCGTTAC8150.013.39263723
GTTACGG12400.013.27822525
Produced by FastQC (version 0.11.5)