##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211935.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2538597
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55703800169936	34.0	31.0	34.0	31.0	34.0
2	32.68618453421319	34.0	31.0	34.0	31.0	34.0
3	32.761519453461894	34.0	31.0	34.0	31.0	34.0
4	36.22255245712494	37.0	37.0	37.0	35.0	37.0
5	36.12833387891028	37.0	35.0	37.0	35.0	37.0
6	36.14544135993228	37.0	36.0	37.0	35.0	37.0
7	36.12675426623446	37.0	36.0	37.0	35.0	37.0
8	36.10969169190699	37.0	35.0	37.0	35.0	37.0
9	37.961549627609266	39.0	38.0	39.0	35.0	39.0
10	37.841878407640124	39.0	38.0	39.0	35.0	39.0
11	37.94014922415807	39.0	38.0	39.0	35.0	39.0
12	37.87848721163698	39.0	38.0	39.0	35.0	39.0
13	37.931297090479504	39.0	38.0	39.0	35.0	39.0
14	39.3271834796937	40.0	39.0	41.0	36.0	41.0
15	39.296452725659094	40.0	39.0	41.0	36.0	41.0
16	39.25239413739164	40.0	39.0	41.0	36.0	41.0
17	39.24304015170584	40.0	39.0	41.0	36.0	41.0
18	39.224169098127824	40.0	39.0	41.0	36.0	41.0
19	39.2353938021671	40.0	39.0	41.0	36.0	41.0
20	39.19204150954248	40.0	39.0	41.0	36.0	41.0
21	39.1828683323899	40.0	39.0	41.0	36.0	41.0
22	39.1601522415728	40.0	39.0	41.0	36.0	41.0
23	39.10200161742884	40.0	39.0	41.0	36.0	41.0
24	39.07551257643494	40.0	39.0	41.0	36.0	41.0
25	39.023752490056516	40.0	39.0	41.0	36.0	41.0
26	38.937092811501785	40.0	38.0	41.0	35.0	41.0
27	38.85943456168899	40.0	38.0	41.0	35.0	41.0
28	38.79605861032689	40.0	38.0	41.0	35.0	41.0
29	38.739734979596996	40.0	38.0	41.0	35.0	41.0
30	38.697407268660605	40.0	38.0	41.0	35.0	41.0
31	38.64881389208291	40.0	38.0	41.0	35.0	41.0
32	38.5965980421469	40.0	38.0	41.0	35.0	41.0
33	38.56605203582924	40.0	38.0	41.0	35.0	41.0
34	38.5162182102949	40.0	38.0	41.0	34.0	41.0
35	38.47705208822038	40.0	38.0	41.0	34.0	41.0
36	38.40705358117102	40.0	38.0	41.0	34.0	41.0
37	38.393816742082336	40.0	38.0	41.0	34.0	41.0
38	38.35051211358085	40.0	38.0	41.0	34.0	41.0
39	38.30652876372264	40.0	38.0	41.0	34.0	41.0
40	38.27053565414282	40.0	38.0	41.0	34.0	41.0
41	38.19640927646255	40.0	38.0	41.0	34.0	41.0
42	38.12270281576792	40.0	38.0	41.0	34.0	41.0
43	37.19896620062184	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	1.0
16	3.0
17	16.0
18	36.0
19	65.0
20	167.0
21	357.0
22	695.0
23	1234.0
24	2252.0
25	3559.0
26	5210.0
27	7578.0
28	10913.0
29	15286.0
30	21189.0
31	28570.0
32	38066.0
33	51045.0
34	72058.0
35	102388.0
36	155417.0
37	260972.0
38	624449.0
39	1137067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83515658452287	16.76532352319017	12.611217928643262	27.788301963643697
2	18.654437864694554	19.090938813840875	35.77507576035109	26.47954756111348
3	20.126707783866443	20.6406136933117	29.40801552983794	29.824662992983917
4	15.830988534217916	14.511558943778788	32.65425745007971	37.003195071923585
5	16.57754263477031	34.4650608190272	33.12408389358374	15.83331265261875
6	37.35354607288987	32.46702804738208	14.530230674660057	15.649195205067995
7	30.961787160388198	29.564952609650135	19.718687133089656	19.754573096872015
8	27.53678508246878	34.645790568569964	18.766507641819477	19.05091670714178
9	28.512717851632218	14.066470574100576	17.4531837861622	39.967627788105005
10	17.441287451296915	25.739059803505636	32.039035735093044	24.7806170101044
11	38.2718091922428	20.351123080977406	19.924824617692373	21.45224310908742
12	22.58483721520194	23.02653788687216	28.876343901769363	25.512280996156537
13	32.269871901684276	18.660425423964497	22.707582180235775	26.362120494115448
14	23.508024314217657	18.920135807298283	23.91998414872467	33.65185572975939
15	27.162286885236213	25.63211096523001	20.908202444105935	26.297399705427843
16	25.766791656966426	24.80819129621598	23.26663113522942	26.158385911588173
17	25.73508122793811	24.229840340944232	23.66984598185533	26.365232449262326
18	25.78672392664137	24.588266668557473	24.110246722894576	25.514762681906582
19	27.2824713808454	24.035520407532193	23.91805394869686	24.763954262925544
20	26.416087311219545	23.432116243736207	23.81078997572281	26.34100646932144
21	25.920577389794442	24.238821679849146	24.48088452007152	25.359716410284893
22	26.96784877631227	23.334030568853585	23.141680227306658	26.556440427527487
23	25.96505077410869	23.762850109725964	24.042295803548182	26.229803312617168
24	25.643377030698456	23.27900017214233	24.23125844708711	26.846364350072104
25	26.314889681190046	23.113554455472844	23.755680795336954	26.81587506800016
26	25.75497410577575	23.496482505888096	24.392725588189066	26.35581780014709
27	26.704711303133188	23.877756099136647	23.710577141625865	25.706955456104296
28	25.861568417515656	24.668822975840595	23.05974520571796	26.409863400925786
29	25.90679024673865	24.42329365393562	24.023978599202632	25.6459375001231
30	26.947483196427	23.734842513404057	23.312522625686547	26.00515166448239
31	26.10607355165077	23.518778285801172	24.04804701179431	26.327101150753744
32	25.52681658412107	23.815753347222895	24.030320685008295	26.627109383647742
33	25.63707433673009	23.12781430057626	24.205299226304923	27.02981213638872
34	26.082477841106723	23.350338789496718	25.081019161371422	25.48616420802514
35	25.94968795756081	22.987067265895295	24.545565916921827	26.51767885962207
36	25.410610664079407	22.525276757200928	25.58417109923316	26.479941479486502
37	24.85790379489143	22.234958916283283	25.975765353854907	26.93137193497038
38	25.282311449985954	21.607368164383715	26.08436864929723	27.0259517363331
39	24.15093849082781	21.87117529879693	27.479627526543204	26.498258683832056
40	25.20002190186154	21.29814224156099	27.365706333065077	26.1361295235124
41	23.941689051078214	21.7606418033268	27.825448466219726	26.472220679375262
42	23.34320886694501	21.962603753175475	28.194865116440305	26.49932226343921
43	22.77990559352272	21.687530553293808	27.734886632261833	27.797677220921635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	50.0
2	59.0
3	145.5
4	232.0
5	232.0
6	281.5
7	331.0
8	313.5
9	296.0
10	418.5
11	541.0
12	541.0
13	911.0
14	1281.0
15	1982.5
16	2684.0
17	2677.5
18	2671.0
19	2671.0
20	3313.0
21	3955.0
22	4639.0
23	5323.0
24	7225.5
25	9128.0
26	9128.0
27	11972.0
28	14816.0
29	18253.0
30	21690.0
31	27148.5
32	32607.0
33	32607.0
34	40010.5
35	47414.0
36	56427.5
37	65441.0
38	77331.5
39	89222.0
40	89222.0
41	103004.0
42	116786.0
43	132919.0
44	149052.0
45	170863.0
46	192674.0
47	192674.0
48	214277.0
49	235880.0
50	250412.5
51	264945.0
52	265408.0
53	265871.0
54	265871.0
55	251823.0
56	237775.0
57	222421.5
58	207068.0
59	191280.5
60	175493.0
61	175493.0
62	154282.0
63	133071.0
64	112430.0
65	91789.0
66	76606.0
67	61423.0
68	61423.0
69	50707.0
70	39991.0
71	33206.0
72	26421.0
73	21886.5
74	17352.0
75	17352.0
76	14165.5
77	10979.0
78	8867.5
79	6756.0
80	5275.5
81	3795.0
82	3795.0
83	2918.0
84	2041.0
85	1552.5
86	1064.0
87	757.5
88	451.0
89	451.0
90	290.5
91	130.0
92	85.0
93	40.0
94	26.5
95	13.0
96	13.0
97	8.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2538597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.75175307914448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83806811374562	52.81072063940863
2	9.256207182459939	11.801988694911794
3	3.052650141980868	5.838353942657389
4	1.4379435985941418	3.666857009572407
5	0.8285356167495879	2.6410299028148203
6	0.5496640688107193	2.1025228794779314
7	0.3763465497510976	1.6794926618343957
8	0.27601992758312743	1.4077403414562306
9	0.21792096232265373	1.2503559042687031
>10	1.0954487258756136	11.953540181055168
>50	0.04959691112702327	2.14324520394125
>100	0.02073184681111263	2.2752003754340575
>500	6.807068624792732E-4	0.2903209514082583
>1k	1.8564732612741052E-4	0.1386313117590146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.93918373022579E-5	0.0
2	0.0	0.0	0.0	3.93918373022579E-5	0.0
3	0.0	0.0	0.0	3.93918373022579E-5	0.0
4	0.0	0.0	0.0	7.87836746045158E-5	0.0
5	0.0	0.0	0.0	7.87836746045158E-5	0.0
6	0.0	0.0	0.0	7.87836746045158E-5	0.0
7	0.0	0.0	0.0	7.87836746045158E-5	0.0
8	0.0	0.0	0.0	7.87836746045158E-5	0.0
9	0.0	0.0	0.0	7.87836746045158E-5	0.0
10	0.0	0.0	0.0	1.181755119067737E-4	0.0
11	0.0	0.0	0.0	1.181755119067737E-4	0.0
12	0.0	0.0	0.0	1.181755119067737E-4	3.93918373022579E-5
13	0.0	0.0	0.0	1.181755119067737E-4	1.181755119067737E-4
14	0.0	0.0	0.0	1.181755119067737E-4	1.575673492090316E-4
15	0.0	0.0	0.0	1.181755119067737E-4	2.363510238135474E-4
16	0.0	0.0	0.0	1.575673492090316E-4	3.545265357203211E-4
17	0.0	0.0	0.0	1.575673492090316E-4	3.545265357203211E-4
18	0.0	0.0	0.0	1.969591865112895E-4	4.3331021032483693E-4
19	0.0	0.0	0.0	1.969591865112895E-4	4.3331021032483693E-4
20	0.0	0.0	0.0	3.151346984180632E-4	4.3331021032483693E-4
21	0.0	0.0	0.0	6.696612341383844E-4	4.3331021032483693E-4
22	0.0	0.0	0.0	0.0011029714444632211	4.3331021032483693E-4
23	0.0	0.0	0.0	0.001575673492090316	6.696612341383844E-4
24	0.0	0.0	0.0	0.002363510238135474	6.696612341383844E-4
25	0.0	0.0	0.0	0.003269522496087406	6.696612341383844E-4
26	0.0	0.0	0.0	0.0055936408969206215	6.696612341383844E-4
27	0.0	0.0	0.0	0.021980645214659908	6.696612341383844E-4
28	0.0	0.0	0.0	0.08098961749344225	6.696612341383844E-4
29	0.0	0.0	0.0	0.17269381473309864	6.696612341383844E-4
30	0.0	0.0	0.0	0.3014657308741797	6.696612341383844E-4
31	0.0	0.0	0.0	0.8822195882213679	7.090530714406422E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2680	0.0	23.125	37
GTAACGT	145	7.2759576E-12	19.137932	26
ATTACGC	60	9.241009E-4	18.5	3
TACTTAC	720	0.0	17.472221	31
GTATTAG	195	0.0	17.076923	1
GGTATCA	2280	0.0	15.90351	1
GTACTTA	840	0.0	15.416668	30
TCTTATA	4625	0.0	15.4	37
TAACGTC	195	4.1836756E-11	15.179487	27
GTATTAA	360	0.0	14.902778	1
TATACAC	720	0.0	14.902778	37
CGTTACG	665	0.0	14.466166	24
TACGGAT	1345	0.0	14.442379	27
CTCGTTA	910	0.0	14.23077	22
TAGACCG	105	1.6568751E-4	14.095239	5
TAAGGCG	145	8.921397E-7	14.034484	5
ACGGATG	1440	0.0	13.875	28
AGTGTAC	1060	0.0	13.613208	27
TCGTTAC	815	0.0	13.392637	23
GTTACGG	1240	0.0	13.278225	25
>>END_MODULE