FastQCFastQC Report
Thu 9 Feb 2017
SRR3211934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4488764
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC42600.019.88967137
TCTTATA74800.016.42245937
TACTTAC9350.015.828876531
GGTATCA38800.015.1623721
CTCGTTA8300.014.48795222
GGACCGT6650.014.4661666
TATACTG5850.013.598295
TCGTTAC7650.013.54248323
CTCTTAT119400.013.54187737
GTATTAG4000.013.41251
GTACTAA2152.906745E-912.9069771
TACACCG2900.012.758625
TACGGAT12950.012.71428527
ACTTACA9050.012.67403332
CGTTACG6000.012.64166624
GTATAAA5050.012.4554441
GTCTTAG6700.012.4253731
TTATACA22000.012.36136437
CTAATAC4100.012.1829273
TAGACAG6450.011.759695
Produced by FastQC (version 0.11.5)