FastQCFastQC Report
Thu 9 Feb 2017
SRR3211933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8455762
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC61250.020.96163237
TACTTAC30900.017.78155331
TCTTATA109250.016.91670437
GTACTTA36050.016.01109530
GGTATCA61250.015.6457141
TACGGAT46300.015.14362827
CTCGTTA33900.014.51622522
CGTTACG24650.014.40973624
TCGTTAC31500.014.21269823
TATACCG3650.014.1917815
ACTTACA28050.014.18003532
AGTGTAC44000.014.08522727
ACGGATG50500.013.99405928
GTGTACT43000.013.76744328
CGGATGG53050.013.70499629
ATGGTTG106750.013.50023432
GATGGTT111950.013.35238931
GTTACGG38750.013.27225825
CTCTTAT174450.013.11808637
GATCTCG40800.012.92279419
Produced by FastQC (version 0.11.5)