##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211933.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8455762
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61345009474013	34.0	31.0	34.0	31.0	34.0
2	32.740318968296414	34.0	31.0	34.0	31.0	34.0
3	32.81959496967866	34.0	31.0	34.0	31.0	34.0
4	36.262644573014235	37.0	37.0	37.0	35.0	37.0
5	36.174335914374126	37.0	35.0	37.0	35.0	37.0
6	36.1886656696345	37.0	37.0	37.0	35.0	37.0
7	36.17655487465234	37.0	36.0	37.0	35.0	37.0
8	36.157810969608654	37.0	36.0	37.0	35.0	37.0
9	38.00893154277521	39.0	38.0	39.0	35.0	39.0
10	37.89598004295769	39.0	38.0	39.0	35.0	39.0
11	37.9849028390345	39.0	38.0	39.0	35.0	39.0
12	37.931118212646005	39.0	38.0	39.0	35.0	39.0
13	37.97778603513202	39.0	38.0	39.0	35.0	39.0
14	39.39979803121233	40.0	39.0	41.0	37.0	41.0
15	39.374263135599136	40.0	39.0	41.0	36.0	41.0
16	39.33657794531114	40.0	39.0	41.0	36.0	41.0
17	39.32927759792672	40.0	39.0	41.0	36.0	41.0
18	39.31321943545715	40.0	39.0	41.0	36.0	41.0
19	39.3323756037599	40.0	39.0	41.0	36.0	41.0
20	39.288033414374716	40.0	39.0	41.0	36.0	41.0
21	39.28129445932844	40.0	39.0	41.0	36.0	41.0
22	39.26141227721405	40.0	39.0	41.0	36.0	41.0
23	39.20536174031388	40.0	39.0	41.0	36.0	41.0
24	39.185729328711005	40.0	39.0	41.0	36.0	41.0
25	39.13499398398394	40.0	39.0	41.0	36.0	41.0
26	39.05697345786223	40.0	39.0	41.0	36.0	41.0
27	38.985603899447504	40.0	39.0	41.0	35.0	41.0
28	38.927411509453556	40.0	39.0	41.0	35.0	41.0
29	38.87368778827975	40.0	38.0	41.0	35.0	41.0
30	38.83961374504155	40.0	38.0	41.0	35.0	41.0
31	38.79035005952154	40.0	38.0	41.0	35.0	41.0
32	38.737756573564866	40.0	38.0	41.0	35.0	41.0
33	38.70789208589362	40.0	38.0	41.0	35.0	41.0
34	38.66746261306787	40.0	38.0	41.0	35.0	41.0
35	38.63208531649779	40.0	38.0	41.0	35.0	41.0
36	38.56646958606451	40.0	38.0	41.0	35.0	41.0
37	38.555957227746006	40.0	38.0	41.0	35.0	41.0
38	38.517654588669835	40.0	38.0	41.0	35.0	41.0
39	38.46915830885496	40.0	38.0	41.0	34.0	41.0
40	38.435838662441064	40.0	38.0	41.0	34.0	41.0
41	38.36312765188992	40.0	38.0	41.0	34.0	41.0
42	38.29710013124778	40.0	38.0	41.0	34.0	41.0
43	37.40566704692019	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	1.0
13	6.0
14	2.0
15	7.0
16	17.0
17	43.0
18	85.0
19	191.0
20	445.0
21	952.0
22	1876.0
23	3812.0
24	6490.0
25	10502.0
26	15766.0
27	23040.0
28	34027.0
29	47053.0
30	65154.0
31	88322.0
32	117355.0
33	157882.0
34	222050.0
35	317737.0
36	478720.0
37	815289.0
38	2007109.0
39	4041823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94012769044351	16.838612534269533	12.494332267156999	27.72692750812996
2	17.687099045597545	19.15852172755099	37.22719489976185	25.927184327089627
3	19.55726757683104	21.372562283564744	29.717771148241873	29.35239899136234
4	15.023814530257592	14.639520364929856	33.86074489797608	36.47592020683648
5	15.748125361144272	35.4900007828981	33.6887438411819	15.073130014775723
6	36.47105961591634	33.52897113234739	15.04298489006668	14.956984361669592
7	30.355596574264982	30.411984159440625	20.33790686161697	18.894512404677428
8	27.120252438514708	36.01476720844319	18.919796938466337	17.945183414575766
9	28.522763530950847	14.488227081131186	17.5843525397238	39.404656848194165
10	16.62959529844856	26.476691278680736	32.636242600016416	24.257470822854284
11	37.76181259595528	21.01718331239692	20.425929679666954	20.79507441198085
12	22.08722289014284	23.315781593663587	29.836010048532586	24.760985467660987
13	31.47311856696061	19.182209716877082	23.543271440232118	25.801400275930185
14	22.85708845636857	19.145843981890692	24.670999491234497	33.326068070506246
15	26.348163536296315	26.343634080524026	21.54032954096863	25.767872842211027
16	25.093847248775454	25.59913583187417	23.798777685559266	25.508239233791112
17	25.032504462637426	25.027525609164496	24.153506212686686	25.78646371551139
18	25.028436230821065	25.553072567558075	24.77544897786858	24.643042223752275
19	26.790276263688597	24.750188096590232	24.347054706601252	24.112480933119926
20	25.50827471255695	24.159052726413066	24.47505026749807	25.85762229353191
21	25.062436714751435	24.929379516594718	25.383389456798806	24.62479431185504
22	26.081481479729447	23.771518167138574	23.866033599337353	26.280966753794633
23	25.120349886858218	24.40626876678885	25.095928669704755	25.377452676648183
24	24.796062140821846	23.865040193893822	25.106726040775506	26.232171624508826
25	25.56818652180608	23.86673134839888	24.535565215766482	26.029516914028566
26	25.065345973550347	24.12681435451944	25.175909634164256	25.631930037765965
27	25.915239809256697	24.69537340336684	24.363883467864873	25.025503319511593
28	25.148413590637958	25.462057706922213	23.593734071512422	25.79579463092741
29	25.22364040047485	25.276113495152774	24.588085615465523	24.912160488906853
30	26.609251774115684	24.344228231589298	23.818775883237965	25.227744111057053
31	25.562498093016334	24.310440620253974	24.69270066967353	25.434360617056157
32	24.997711619603297	24.75870300039192	24.551601617926334	25.69198376207845
33	24.9778316844774	23.88648119471669	24.733205594007966	26.40248152679794
34	25.489258094066507	24.175798703889726	25.55749558703284	24.777447615010924
35	25.371776074113722	23.85405360273858	24.90504108322822	25.869129239919474
36	24.980196935533428	23.62169133899464	25.716771593145598	25.681340132326337
37	24.39611001350322	23.510110620426637	26.193334202168888	25.900445163901253
38	25.299754179457747	22.829131188886347	25.785304742493935	26.085809889161972
39	23.8854050054862	23.337707470952942	27.140309767469805	25.636577756091057
40	25.248629277881758	22.515238721241207	26.90311056531629	25.33302143556074
41	23.986933407066093	23.124905833442334	27.159160818386326	25.728999941105247
42	23.70611897543947	23.235564104098483	27.470546119912083	25.58777080054997
43	23.086032932336554	22.879759387740574	26.941403979913343	27.092803700009533
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	185.0
1	243.0
2	301.0
3	716.0
4	1131.0
5	1131.0
6	1417.5
7	1704.0
8	1605.0
9	1506.0
10	2084.5
11	2663.0
12	2663.0
13	4367.0
14	6071.0
15	9299.0
16	12527.0
17	12988.5
18	13450.0
19	13450.0
20	17135.5
21	20821.0
22	24357.5
23	27894.0
24	36890.0
25	45886.0
26	45886.0
27	58118.0
28	70350.0
29	84644.5
30	98939.0
31	120316.5
32	141694.0
33	141694.0
34	169084.5
35	196475.0
36	229398.0
37	262321.0
38	302258.5
39	342196.0
40	342196.0
41	387674.5
42	433153.0
43	484605.5
44	536058.0
45	603433.5
46	670809.0
47	670809.0
48	732007.5
49	793206.0
50	835182.0
51	877158.0
52	868920.5
53	860683.0
54	860683.0
55	806010.0
56	751337.0
57	695727.0
58	640117.0
59	589322.0
60	538527.0
61	538527.0
62	468855.5
63	399184.0
64	333376.0
65	267568.0
66	220107.0
67	172646.0
68	172646.0
69	140046.0
70	107446.0
71	87085.5
72	66725.0
73	53894.5
74	41064.0
75	41064.0
76	32693.0
77	24322.0
78	19459.0
79	14596.0
80	11267.0
81	7938.0
82	7938.0
83	5982.5
84	4027.0
85	3005.5
86	1984.0
87	1377.0
88	770.0
89	770.0
90	496.0
91	222.0
92	142.5
93	63.0
94	46.5
95	30.0
96	30.0
97	20.5
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8455762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.861381864497865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14140042441483	38.46376824123885
2	11.557202663455188	11.525161905758619
3	3.989209546572914	5.967225016175174
4	2.0240497173776433	4.036876634835827
5	1.1416062119666737	2.84610316368766
6	0.7846742867792146	2.3474966551410557
7	0.555170373703961	1.9377093402176433
8	0.4022234987344041	1.60443355722164
9	0.3188484949064131	1.430840390530416
>10	1.8780568080564493	17.484708564925878
>50	0.14510579114030753	4.925367906010304
>100	0.05819004416655993	5.179900155344699
>500	0.0028576502098773457	0.9602115445996219
>1k	0.0013806836306925066	1.2307310063178858
>5k	2.380488519505279E-5	0.05946591799464806
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3652510560254654E-5	7.095753168076396E-5
2	0.0	0.0	0.0	2.3652510560254654E-5	7.095753168076396E-5
3	0.0	0.0	0.0	2.3652510560254654E-5	7.095753168076396E-5
4	0.0	0.0	0.0	2.3652510560254654E-5	8.278378696089128E-5
5	0.0	0.0	0.0	2.3652510560254654E-5	8.278378696089128E-5
6	0.0	0.0	0.0	2.3652510560254654E-5	1.0643629752114594E-4
7	0.0	0.0	0.0	2.3652510560254654E-5	1.0643629752114594E-4
8	0.0	0.0	0.0	2.3652510560254654E-5	1.300888080814006E-4
9	0.0	0.0	0.0	2.3652510560254654E-5	1.419150633615279E-4
10	0.0	0.0	0.0	8.278378696089128E-5	1.419150633615279E-4
11	0.0	0.0	0.0	1.1826255280127326E-4	1.419150633615279E-4
12	0.0	0.0	0.0	1.5374131864165523E-4	2.4835136088267385E-4
13	0.0	0.0	0.0	1.5374131864165523E-4	2.601776161628012E-4
14	0.0	0.0	0.0	1.7739382920190988E-4	3.193088925634378E-4
15	0.0	1.1826255280127327E-5	0.0	1.7739382920190988E-4	4.020926795243291E-4
16	0.0	1.1826255280127327E-5	0.0	2.246988503224192E-4	4.1391893480445643E-4
17	0.0	1.1826255280127327E-5	0.0	2.9565638200318314E-4	4.2574519008458376E-4
18	0.0	1.1826255280127327E-5	0.0	2.9565638200318314E-4	4.967027217653477E-4
19	0.0	1.1826255280127327E-5	0.0	3.5478765840381976E-4	5.085289770454751E-4
20	0.0	1.1826255280127327E-5	0.0	4.848764664852204E-4	5.79486508726239E-4
21	0.0	1.1826255280127327E-5	0.0	6.031390192864937E-4	6.149652745666209E-4
22	0.0	1.1826255280127327E-5	0.0	8.160116143287855E-4	6.267915298467483E-4
23	0.0	1.1826255280127327E-5	0.0	0.001052536719931332	6.386177851268756E-4
24	0.0	1.1826255280127327E-5	0.0	0.0016438494839376983	6.386177851268756E-4
25	1.1826255280127327E-5	1.1826255280127327E-5	0.0	0.00221150973738381	6.386177851268756E-4
26	1.1826255280127327E-5	1.1826255280127327E-5	0.0	0.003512397818197816	6.622702956871302E-4
27	1.1826255280127327E-5	1.1826255280127327E-5	0.0	0.0138485449330291	6.622702956871302E-4
28	1.1826255280127327E-5	1.1826255280127327E-5	0.0	0.054164249182983154	6.622702956871302E-4
29	1.1826255280127327E-5	1.1826255280127327E-5	0.0	0.1176712400372669	6.622702956871302E-4
30	1.1826255280127327E-5	1.1826255280127327E-5	0.0	0.20259557920386123	6.622702956871302E-4
31	1.1826255280127327E-5	1.1826255280127327E-5	0.0	0.643572986089249	6.622702956871302E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6125	0.0	20.961632	37
TACTTAC	3090	0.0	17.781553	31
TCTTATA	10925	0.0	16.916704	37
GTACTTA	3605	0.0	16.011095	30
GGTATCA	6125	0.0	15.645714	1
TACGGAT	4630	0.0	15.143628	27
CTCGTTA	3390	0.0	14.516225	22
CGTTACG	2465	0.0	14.409736	24
TCGTTAC	3150	0.0	14.212698	23
TATACCG	365	0.0	14.191781	5
ACTTACA	2805	0.0	14.180035	32
AGTGTAC	4400	0.0	14.085227	27
ACGGATG	5050	0.0	13.994059	28
GTGTACT	4300	0.0	13.767443	28
CGGATGG	5305	0.0	13.704996	29
ATGGTTG	10675	0.0	13.500234	32
GATGGTT	11195	0.0	13.352389	31
GTTACGG	3875	0.0	13.272258	25
CTCTTAT	17445	0.0	13.118086	37
GATCTCG	4080	0.0	12.922794	19
>>END_MODULE