##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211932.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7313177
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59518031629755	34.0	31.0	34.0	31.0	34.0
2	32.72089941211596	34.0	31.0	34.0	31.0	34.0
3	32.80024153661261	34.0	31.0	34.0	31.0	34.0
4	36.25094716017403	37.0	37.0	37.0	35.0	37.0
5	36.16108389007951	37.0	35.0	37.0	35.0	37.0
6	36.17728765487284	37.0	36.0	37.0	35.0	37.0
7	36.16273064360401	37.0	36.0	37.0	35.0	37.0
8	36.14571409935791	37.0	36.0	37.0	35.0	37.0
9	37.994553940100175	39.0	38.0	39.0	35.0	39.0
10	37.880969378971685	39.0	38.0	39.0	35.0	39.0
11	37.97357550624031	39.0	38.0	39.0	35.0	39.0
12	37.91751437166091	39.0	38.0	39.0	35.0	39.0
13	37.96519720499039	39.0	38.0	39.0	35.0	39.0
14	39.3818914816365	40.0	39.0	41.0	36.0	41.0
15	39.35261063693659	40.0	39.0	41.0	36.0	41.0
16	39.318493590405375	40.0	39.0	41.0	36.0	41.0
17	39.30665947234697	40.0	39.0	41.0	36.0	41.0
18	39.295167886679074	40.0	39.0	41.0	36.0	41.0
19	39.305535337104516	40.0	39.0	41.0	36.0	41.0
20	39.26527909826331	40.0	39.0	41.0	36.0	41.0
21	39.25825670566978	40.0	39.0	41.0	36.0	41.0
22	39.235393728334486	40.0	39.0	41.0	36.0	41.0
23	39.18142593841227	40.0	39.0	41.0	36.0	41.0
24	39.15899150259867	40.0	39.0	41.0	36.0	41.0
25	39.11150516389799	40.0	39.0	41.0	36.0	41.0
26	39.03000159848449	40.0	39.0	41.0	36.0	41.0
27	38.95755565604388	40.0	39.0	41.0	35.0	41.0
28	38.900360677719135	40.0	38.0	41.0	35.0	41.0
29	38.8482927460938	40.0	38.0	41.0	35.0	41.0
30	38.80566982038039	40.0	38.0	41.0	35.0	41.0
31	38.7607317585777	40.0	38.0	41.0	35.0	41.0
32	38.70677586499001	40.0	38.0	41.0	35.0	41.0
33	38.676633151364996	40.0	38.0	41.0	35.0	41.0
34	38.63278722776708	40.0	38.0	41.0	35.0	41.0
35	38.59163862709736	40.0	38.0	41.0	35.0	41.0
36	38.53014811483436	40.0	38.0	41.0	35.0	41.0
37	38.51201618667236	40.0	38.0	41.0	35.0	41.0
38	38.46804829146074	40.0	38.0	41.0	34.0	41.0
39	38.426200405104375	40.0	38.0	41.0	34.0	41.0
40	38.3853388205974	40.0	38.0	41.0	34.0	41.0
41	38.31037755547281	40.0	38.0	41.0	34.0	41.0
42	38.23650952246883	40.0	38.0	41.0	34.0	41.0
43	37.34139950393652	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	1.0
13	1.0
14	2.0
15	10.0
16	19.0
17	39.0
18	82.0
19	182.0
20	418.0
21	854.0
22	1732.0
23	3306.0
24	5769.0
25	9063.0
26	13779.0
27	20518.0
28	29645.0
29	41541.0
30	58183.0
31	78136.0
32	104570.0
33	140861.0
34	195949.0
35	280198.0
36	421909.0
37	720539.0
38	1739246.0
39	3446622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.961383267490994	17.00851763877724	12.520604929977766	27.509494163754
2	17.76743541144977	19.1843435486383	36.83910563083595	26.209115409075974
3	19.59811720678988	21.18488312261552	29.653199970409577	29.563799700185022
4	14.936559035833538	14.663668061090277	33.90738389074953	36.492389012326655
5	15.79056544098413	35.21950856652314	33.7542356762321	15.235690316260635
6	36.43397390764643	33.679589595602565	14.988451667449043	14.897984829301958
7	30.57627348551799	30.093966001369854	20.249708163770684	19.08005234934147
8	27.262173471256062	35.5087399087975	18.91790667722113	18.311179942725303
9	28.011396414991736	14.516208208826342	17.776241433784524	39.6961539423974
10	16.843760242641466	26.23103474727878	32.57385948678666	24.35134552329309
11	37.7411075925005	20.84757418014086	20.532881947202974	20.878436280155668
12	22.218729288242304	23.397751756862988	29.75652031941795	24.626998635476756
13	31.591044494068722	19.010055410938364	23.56603703151175	25.832863063481167
14	22.95915988359095	19.01619501346679	24.678631462085495	33.34601364085677
15	26.157756061421733	26.20799414536254	21.584449549081064	26.049800244134662
16	25.290349187500865	25.351252950666996	23.615782853334466	25.742615008497676
17	25.07661171061496	24.97690128380593	24.307438477148853	25.63904852843026
18	25.239276992748845	25.045571849279728	24.760620452643224	24.954530705328203
19	26.54125559931067	24.588820973429197	24.525196094665837	24.344727332594303
20	25.787451883087197	24.1058161179471	24.463034328309025	25.643697670656678
21	25.295189764995435	24.67087833372555	25.042399493407586	24.991532407871436
22	26.054804909001927	23.910962362869107	24.07957581226326	25.954656915865705
23	25.334461342860976	24.271708451744022	24.802818255321863	25.591011950073135
24	25.110946993351863	23.918182207267787	24.84518014537321	26.125690654007144
25	25.678949107891142	23.869530301263048	24.532593700384936	25.918926890460874
26	25.305185420782237	24.150147056470807	24.8978932138522	25.646774308894756
27	25.87109542131963	24.539088825554202	24.413507289649903	25.17630846347627
28	25.384740448644962	25.084651991877127	23.851617429743598	25.678990129734313
29	25.427307447912174	24.954790510334977	24.47248302618684	25.145419015566013
30	26.15517168530175	24.443576300696673	24.111559175991502	25.289692838010076
31	25.63135009586121	24.150215426209428	24.589272213704113	25.629162264225247
32	24.98788146382892	24.571509755609636	24.56794085525347	25.872667925307972
33	25.076051078758248	23.95838361357861	24.81381210929258	26.15175319837056
34	25.47487911204665	24.056822363249243	25.338727614551104	25.129570910153003
35	25.361604129094644	23.920971692603636	24.953327397928422	25.76409678037329
36	25.07246850445436	23.58721797653742	25.598806100276256	25.741507418731967
37	24.737155958347515	23.373808674396916	25.847972775717036	26.041062591538534
38	25.026264235092356	22.989051133317297	25.85390726902959	26.13077736256076
39	24.1863009742551	23.085985748738203	26.891459074489788	25.836254202516905
40	25.031214204168723	22.657840771527887	26.809196604977565	25.501748419325825
41	24.034369741085168	22.963508198967425	27.17494735871975	25.827174701227662
42	23.678997513666086	23.125831632408186	27.387782355055812	25.807388498869916
43	23.26723392583005	22.773098476899985	27.14409893265266	26.815568664617306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	175.0
1	221.0
2	267.0
3	681.5
4	1096.0
5	1096.0
6	1434.5
7	1773.0
8	1657.0
9	1541.0
10	2158.5
11	2776.0
12	2776.0
13	4555.5
14	6335.0
15	10099.0
16	13863.0
17	14003.0
18	14143.0
19	14143.0
20	17126.0
21	20109.0
22	22296.5
23	24484.0
24	31907.0
25	39330.0
26	39330.0
27	49524.0
28	59718.0
29	72306.0
30	84894.0
31	102679.0
32	120464.0
33	120464.0
34	143346.5
35	166229.0
36	193914.0
37	221599.0
38	256295.5
39	290992.0
40	290992.0
41	331560.5
42	372129.0
43	417307.5
44	462486.0
45	519042.0
46	575598.0
47	575598.0
48	626943.0
49	678288.0
50	702971.5
51	727655.0
52	723850.0
53	720045.0
54	720045.0
55	686382.0
56	652719.0
57	610266.0
58	567813.0
59	522729.5
60	477646.0
61	477646.0
62	418058.0
63	358470.0
64	300623.0
65	242776.0
66	200538.5
67	158301.0
68	158301.0
69	129068.0
70	99835.0
71	80592.5
72	61350.0
73	49891.5
74	38433.0
75	38433.0
76	30481.5
77	22530.0
78	18081.5
79	13633.0
80	10468.5
81	7304.0
82	7304.0
83	5489.5
84	3675.0
85	2699.0
86	1723.0
87	1210.0
88	697.0
89	697.0
90	449.5
91	202.0
92	125.0
93	48.0
94	34.5
95	21.0
96	21.0
97	15.5
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7313177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.700861660495455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5280839005746	40.082687405468924
2	11.516467638347057	11.90822600375508
3	3.9410487493843043	6.112668485675597
4	1.8955343511944622	3.920030370552875
5	1.1265127108355997	2.912083891085053
6	0.7615195827312113	2.3622731159126746
7	0.5341888941347389	1.933261828136323
8	0.39381465454732273	1.6288445579701554
9	0.30207991196027406	1.4056012564805498
>10	1.8226284345972663	17.509964433610744
>50	0.12648050168141323	4.426431702469731
>100	0.04891191161153022	4.453826135742419
>500	0.00211948243270962	0.7426818814630446
>1k	5.827857088127755E-4	0.5178752107414313
>5k	2.649025860838205E-5	0.08354372093542525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3673947724771327E-5
2	0.0	0.0	0.0	0.0	1.3673947724771327E-5
3	0.0	0.0	0.0	0.0	2.7347895449542654E-5
4	0.0	0.0	0.0	1.3673947724771327E-5	2.7347895449542654E-5
5	0.0	0.0	0.0	1.3673947724771327E-5	2.7347895449542654E-5
6	1.3673947724771327E-5	0.0	0.0	1.3673947724771327E-5	2.7347895449542654E-5
7	1.3673947724771327E-5	0.0	0.0	4.102184317431398E-5	4.102184317431398E-5
8	1.3673947724771327E-5	0.0	0.0	4.102184317431398E-5	4.102184317431398E-5
9	1.3673947724771327E-5	0.0	0.0	1.0939158179817062E-4	4.102184317431398E-5
10	1.3673947724771327E-5	0.0	0.0	1.2306552952294193E-4	5.469579089908531E-5
11	1.3673947724771327E-5	0.0	0.0	1.2306552952294193E-4	5.469579089908531E-5
12	1.3673947724771327E-5	0.0	0.0	1.3673947724771328E-4	1.0939158179817062E-4
13	1.3673947724771327E-5	1.3673947724771327E-5	0.0	1.3673947724771328E-4	1.0939158179817062E-4
14	1.3673947724771327E-5	1.3673947724771327E-5	0.0	1.504134249724846E-4	1.2306552952294193E-4
15	1.3673947724771327E-5	1.3673947724771327E-5	0.0	1.504134249724846E-4	1.9143526814679857E-4
16	1.3673947724771327E-5	1.3673947724771327E-5	0.0	1.9143526814679857E-4	2.4613105904588387E-4
17	1.3673947724771327E-5	1.3673947724771327E-5	0.0	2.4613105904588387E-4	2.4613105904588387E-4
18	1.3673947724771327E-5	1.3673947724771327E-5	0.0	2.598050067706552E-4	3.008268499449692E-4
19	1.3673947724771327E-5	1.3673947724771327E-5	0.0	3.555226408440545E-4	3.008268499449692E-4
20	1.3673947724771327E-5	1.3673947724771327E-5	0.0	4.238923794679111E-4	3.4184869311928317E-4
21	1.3673947724771327E-5	1.3673947724771327E-5	0.0	6.70023438513795E-4	3.555226408440545E-4
22	1.3673947724771327E-5	1.3673947724771327E-5	0.0	8.204368634862797E-4	3.8287053629359715E-4
23	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.0010665679225321635	4.238923794679111E-4
24	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.0016135258315230167	4.3756632719268247E-4
25	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.0021604837405138698	4.512402749174538E-4
26	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.0035005306175414598	4.512402749174538E-4
27	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.014617450117780548	4.512402749174538E-4
28	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.0545590514218376	4.512402749174538E-4
29	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.11844373519196924	4.512402749174538E-4
30	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.20633987116679933	4.512402749174538E-4
31	1.3673947724771327E-5	2.7347895449542654E-5	0.0	0.6143157754830767	4.512402749174538E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5305	0.0	20.819038	37
TACTTAC	1895	0.0	17.474934	31
TATACCG	315	0.0	17.031746	5
TCTTATA	9135	0.0	15.674877	37
GGTATCA	5525	0.0	15.268779	1
CTCGTTA	1625	0.0	15.255384	22
TCGTTAC	1610	0.0	14.248447	23
GTACTTA	2420	0.0	13.760331	30
TTATACT	895	0.0	13.642459	4
AGTGTAC	2900	0.0	12.95	27
CGTTACA	800	0.0	12.718751	24
GTATTAG	670	0.0	12.701492	1
TACGGAT	2535	0.0	12.698224	27
CGTTACG	1150	0.0	12.547827	24
TCTCGTT	2000	0.0	12.4875	21
GTACTAG	550	0.0	12.445455	1
TTACGGG	790	0.0	12.411392	26
ACGGATG	2650	0.0	12.356604	28
ATGGTTG	6530	0.0	12.352221	32
GATCTCG	2355	0.0	12.333333	19
>>END_MODULE