FastQCFastQC Report
Thu 9 Feb 2017
SRR3211931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6975354
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC47200.020.812537
GGTATCA45800.016.5207421
TCTTATA83200.016.20973637
TACTTAC17100.015.4707631
TATACCG3550.014.0704235
TCGTTAC17100.013.84795323
CTCGTTA16500.013.7909122
GTACTTA20000.013.597530
CGTTACG11750.012.91063924
GTATTAG7250.012.7586211
TACGGAT25000.012.72827
CTCTTAT129050.012.70127837
GGACCGT7500.012.5799996
GTCTTAG10400.012.4519231
TAGACAG13550.012.1512915
GTATCAA63300.012.0410742
GGGTAAG50350.011.9781521
ACGGATG27250.011.94862428
TTACGGA27550.011.68421126
ACTTACA17400.011.58908132
Produced by FastQC (version 0.11.5)