FastQCFastQC Report
Thu 9 Feb 2017
SRR3211930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5871088
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC43200.021.3263937
TACTTAC20300.017.04187231
TCTTATA77350.016.90950237
GGTATCA46300.014.8239741
GTACTTA25250.014.5069330
TCGTTAC19650.014.02798923
AGTGTAC31100.013.741158527
CGTTACG15450.013.530745524
CTCGTTA20000.013.227522
TTCGTTA4800.013.10416722
GTGTACT30800.012.9139628
TATACTA3750.012.8266665
TACGGAT36950.012.81732127
CTCTTAT119350.012.78801737
TATACTG8150.012.7116585
ACGGATG37700.012.56233428
CAGTGTA35050.012.56205526
ACTTACA19050.012.5275632
CTACAGT36200.012.46961323
TAGTACT4750.012.4631584
Produced by FastQC (version 0.11.5)