##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211928.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2057194
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.484219767314116	34.0	31.0	34.0	31.0	34.0
2	32.61802630184611	34.0	31.0	34.0	31.0	34.0
3	32.69798327236031	34.0	31.0	34.0	31.0	34.0
4	36.17585847518513	37.0	35.0	37.0	35.0	37.0
5	36.06905863034794	37.0	35.0	37.0	35.0	37.0
6	36.0884447456098	37.0	35.0	37.0	35.0	37.0
7	36.074122810002365	37.0	35.0	37.0	35.0	37.0
8	36.05036763669348	37.0	35.0	37.0	35.0	37.0
9	37.896474032103924	39.0	38.0	39.0	35.0	39.0
10	37.77425609835533	39.0	37.0	39.0	35.0	39.0
11	37.8735155750989	39.0	38.0	39.0	35.0	39.0
12	37.80745180085106	39.0	38.0	39.0	35.0	39.0
13	37.863832482498005	39.0	38.0	39.0	35.0	39.0
14	39.23315593959539	40.0	39.0	41.0	36.0	41.0
15	39.19805521501618	40.0	39.0	41.0	36.0	41.0
16	39.156749922467206	40.0	39.0	41.0	36.0	41.0
17	39.134127845988274	40.0	39.0	41.0	36.0	41.0
18	39.127075521316904	40.0	39.0	41.0	36.0	41.0
19	39.12831312943748	40.0	39.0	41.0	36.0	41.0
20	39.09451709464445	40.0	39.0	41.0	36.0	41.0
21	39.0798082242122	40.0	39.0	41.0	36.0	41.0
22	39.05243064096045	40.0	39.0	41.0	36.0	41.0
23	38.99045787611669	40.0	39.0	41.0	36.0	41.0
24	38.95792375439555	40.0	38.0	41.0	35.0	41.0
25	38.90787110987102	40.0	38.0	41.0	35.0	41.0
26	38.81141059132002	40.0	38.0	41.0	35.0	41.0
27	38.72555043423226	40.0	38.0	41.0	35.0	41.0
28	38.669891123540125	40.0	38.0	41.0	35.0	41.0
29	38.60860667491739	40.0	38.0	41.0	35.0	41.0
30	38.54924863673528	40.0	38.0	41.0	34.0	41.0
31	38.50551090465945	40.0	38.0	41.0	34.0	41.0
32	38.45705752593095	40.0	38.0	41.0	34.0	41.0
33	38.420099416972825	40.0	38.0	41.0	34.0	41.0
34	38.36930012434413	40.0	38.0	41.0	34.0	41.0
35	38.316498103727696	40.0	38.0	41.0	34.0	41.0
36	38.2534204357975	40.0	38.0	41.0	34.0	41.0
37	38.232681506945866	40.0	38.0	41.0	34.0	41.0
38	38.1783191084555	40.0	38.0	41.0	34.0	41.0
39	38.140267762787566	40.0	38.0	41.0	34.0	41.0
40	38.09326247305796	40.0	38.0	41.0	33.0	41.0
41	38.01718457277243	40.0	38.0	41.0	33.0	41.0
42	37.932545010339325	40.0	37.0	41.0	33.0	41.0
43	36.97569650698962	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	4.0
16	4.0
17	12.0
18	36.0
19	64.0
20	144.0
21	311.0
22	666.0
23	1179.0
24	1921.0
25	3079.0
26	4740.0
27	6881.0
28	9911.0
29	13549.0
30	18648.0
31	24713.0
32	33276.0
33	44525.0
34	61918.0
35	88033.0
36	133551.0
37	228531.0
38	534125.0
39	847372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.303256766255394	16.59289303779809	12.438642150424316	27.6652080455222
2	18.721229013889793	18.95747314059831	35.322628784645495	26.998669060866405
3	20.447512485453487	20.571710786634608	28.798013216060326	30.18276351185158
4	15.594397028184995	14.336372748510836	32.67285438320353	37.396375840100646
5	16.859664183348773	34.046521621198586	32.96295828200938	16.130855913443263
6	37.683660364554825	32.437290795131624	14.35746944624571	15.521579394067842
7	31.454593003868375	28.856393709100843	19.64457411406022	20.04443917297056
8	28.013352168050265	34.02362635706695	18.686861812741046	19.276159662141733
9	27.899750825639195	14.203473274761642	17.26351525427354	40.63326064532563
10	17.665810808314628	25.34442546497802	31.981670177921966	25.008093548785382
11	38.46132158658833	20.002537436916498	19.805618721423453	21.730522255071712
12	22.927929986185067	23.03088575992347	28.73642446944722	25.304759784444247
13	32.80939959964884	18.18462429892368	22.597820137527137	26.40815596390034
14	23.571379267098777	18.56008718672133	23.997445063518562	33.871088482661335
15	27.372576431780377	25.162527209392987	20.833523722118574	26.63137263670806
16	26.287554795512726	24.110657526708714	23.002497576796355	26.59929010098221
17	25.903731004465307	24.123101661778133	23.772770093632396	26.20039724012417
18	26.224750801334245	23.749048461156313	24.073179291792606	25.953021445716836
19	27.15174164420079	23.82842843212648	23.84699741492538	25.17283250874735
20	26.789792309330085	23.22756142590344	23.607107545520744	26.37553871924573
21	26.208272044347787	23.996424255563646	23.836935165084093	25.958368535004478
22	26.8725749734833	23.479992650182723	23.02850387469534	26.61892850163864
23	26.340102100239456	23.33406572253273	23.544789650368415	26.7810425268594
24	26.266215048264772	23.28603913874919	23.632190255270043	26.815555557715996
25	26.713328932516816	22.97478993230585	23.385932488622853	26.92594864655448
26	26.33723411598517	23.39429339187262	23.862844243177843	26.405628248964362
27	26.984183309887154	23.496811676487486	23.55767127456137	25.961333739063985
28	26.37101799830254	24.110414477195636	23.16927815266815	26.349289371833674
29	26.494049661820906	23.91398186072874	23.496617236877025	26.095351240573322
30	26.503334153220358	23.792311274483595	23.61449625071821	26.089858321577843
31	26.409760090686635	23.25701902688808	23.50638782730263	26.826833055122655
32	25.584850043311423	23.48636054742528	23.871350976135457	27.057438433127846
33	25.963569794584274	23.252012206918746	24.00347269144281	26.78094530705417
34	26.340490979460373	23.106960257515823	24.39128249450465	26.161266268519157
35	26.078143335047642	23.15202163723985	24.38802563102945	26.381809396683053
36	25.738457335574573	22.69508855265959	24.876992641432942	26.689461470332894
37	25.3599806338148	22.252592609155965	25.10920214622442	27.278224610804813
38	25.341168601502822	21.905663734193276	25.675556121590866	27.077611542713036
39	24.707003811988564	21.63962173718181	26.55627033716801	27.09710411366162
40	25.224699274837477	21.591352103885196	26.851915764871958	26.332032856405373
41	24.485731535285442	21.495833645246876	27.31020992672543	26.70822489274225
42	23.694508150422372	21.777090541776808	27.71702620170971	26.811375106091113
43	23.456514067219718	21.4190299991153	27.63011169583423	27.494344237830752
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	30.5
2	37.0
3	103.0
4	169.0
5	169.0
6	227.0
7	285.0
8	275.0
9	265.0
10	344.5
11	424.0
12	424.0
13	786.0
14	1148.0
15	1806.0
16	2464.0
17	2333.0
18	2202.0
19	2202.0
20	2603.0
21	3004.0
22	3282.5
23	3561.0
24	4827.5
25	6094.0
26	6094.0
27	8041.0
28	9988.0
29	12496.5
30	15005.0
31	19178.0
32	23351.0
33	23351.0
34	29027.0
35	34703.0
36	41470.5
37	48238.0
38	57750.5
39	67263.0
40	67263.0
41	78349.5
42	89436.0
43	103074.0
44	116712.0
45	133062.0
46	149412.0
47	149412.0
48	167482.5
49	185553.0
50	196655.5
51	207758.0
52	213714.0
53	219670.0
54	219670.0
55	212283.0
56	204896.0
57	190490.5
58	176085.0
59	162670.0
60	149255.0
61	149255.0
62	131259.5
63	113264.0
64	96337.0
65	79410.0
66	66414.0
67	53418.0
68	53418.0
69	43965.0
70	34512.0
71	28923.5
72	23335.0
73	19072.0
74	14809.0
75	14809.0
76	12083.0
77	9357.0
78	7570.0
79	5783.0
80	4532.0
81	3281.0
82	3281.0
83	2495.0
84	1709.0
85	1263.0
86	817.0
87	583.5
88	350.0
89	350.0
90	227.5
91	105.0
92	64.0
93	23.0
94	17.5
95	12.0
96	12.0
97	8.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2057194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.74269123693705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.8408227561402	55.119213235076906
2	9.076061620519871	11.933694335305047
3	2.8040688641441913	5.53041100627621
4	1.2997917378122805	3.418072275652583
5	0.725585401132799	2.3850968496351355
6	0.5084057251329587	2.0054376366304307
7	0.3298656282300647	1.5180377902485187
8	0.24326687634130625	1.2794415311584515
9	0.18536226506908218	1.0967592743474355
>10	0.9203213663431553	10.370998925748019
>50	0.041865545870398715	1.878162164915686
>100	0.023250484013563257	2.7025881127250355
>500	8.878195000948091E-4	0.37822232838224046
>1k	4.4390975004738323E-4	0.3838645338982159
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.86099026149211E-5
6	0.0	0.0	0.0	0.0	1.944396104596844E-4
7	0.0	0.0	0.0	0.0	1.944396104596844E-4
8	0.0	0.0	0.0	0.0	2.9165941568952663E-4
9	0.0	0.0	0.0	0.0	3.888792209193688E-4
10	0.0	0.0	0.0	0.0	3.888792209193688E-4
11	0.0	0.0	0.0	0.0	3.888792209193688E-4
12	4.86099026149211E-5	0.0	0.0	0.0	5.347089287641321E-4
13	4.86099026149211E-5	0.0	0.0	0.0	5.833188313790533E-4
14	9.72198052298422E-5	0.0	0.0	0.0	6.805386366088954E-4
15	9.72198052298422E-5	0.0	0.0	9.72198052298422E-5	7.291485392238165E-4
16	9.72198052298422E-5	0.0	0.0	1.944396104596844E-4	0.0010208079549133432
17	9.72198052298422E-5	0.0	0.0	2.4304951307460552E-4	0.0010208079549133432
18	9.72198052298422E-5	0.0	0.0	3.402693183044477E-4	0.0011180277601431853
19	9.72198052298422E-5	0.0	0.0	4.8609902614921103E-4	0.0011666376627581065
20	9.72198052298422E-5	0.0	0.0	6.319287339939743E-4	0.0013610772732177909
21	9.72198052298422E-5	0.0	0.0	0.0011180277601431853	0.0013610772732177909
22	9.72198052298422E-5	0.0	0.0	0.0015555168836774752	0.0013610772732177909
23	9.72198052298422E-5	0.0	0.0	0.0018471762993670017	0.001458297078447633
24	9.72198052298422E-5	0.0	0.0	0.0025763248385908184	0.001458297078447633
25	9.72198052298422E-5	0.0	0.0	0.003256863475199714	0.001458297078447633
26	9.72198052298422E-5	0.0	0.0	0.0055415288981010054	0.001458297078447633
27	9.72198052298422E-5	0.0	0.0	0.019152301630278912	0.001458297078447633
28	9.72198052298422E-5	0.0	0.0	0.07208848557792799	0.001458297078447633
29	9.72198052298422E-5	0.0	0.0	0.15812801320633835	0.001458297078447633
30	9.72198052298422E-5	0.0	0.0	0.2754723181187579	0.001458297078447633
31	9.72198052298422E-5	0.0	0.0	0.7520438033554444	0.001458297078447633
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1675	0.0	21.316418	37
TAAGACG	45	0.0038268203	20.555555	4
TTATACG	50	0.007036724	18.5	4
TAGTCCG	65	0.0015805081	17.076921	20
TCTTATA	3000	0.0	16.403332	37
GTCTTAC	160	6.311893E-10	16.1875	1
TATACCG	115	1.2432847E-6	16.086956	5
TAGAGTA	130	2.591678E-7	15.653845	5
GGACCGT	220	1.8189894E-12	15.136364	6
TATAAGT	125	2.961311E-6	14.800001	4
TTAGGCA	225	1.8189894E-12	14.8	4
GTTATAC	115	2.211411E-5	14.478261	3
TAGGACT	220	1.8189894E-11	14.295454	4
TCCTACG	210	1.364242E-10	14.095238	9
ATTAGGT	80	0.0063008503	13.875001	3
TCTATAC	160	1.7853017E-7	13.875001	3
ATCTATA	175	3.5699486E-8	13.742857	2
TAGATAG	135	6.572536E-6	13.703703	5
GTATTAG	275	0.0	13.454546	1
CTCTTAT	4580	0.0	13.370087	37
>>END_MODULE