##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211927.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1994112
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2540268550613	33.0	31.0	34.0	30.0	34.0
2	32.398069917838114	34.0	31.0	34.0	30.0	34.0
3	32.47200107115348	34.0	31.0	34.0	30.0	34.0
4	36.024508151999484	37.0	35.0	37.0	35.0	37.0
5	35.865401241254254	37.0	35.0	37.0	35.0	37.0
6	35.8801712240837	37.0	35.0	37.0	35.0	37.0
7	35.863798522851276	37.0	35.0	37.0	35.0	37.0
8	35.83570030168817	37.0	35.0	37.0	35.0	37.0
9	37.656206873034215	39.0	37.0	39.0	35.0	39.0
10	37.49294121894859	39.0	37.0	39.0	35.0	39.0
11	37.61205890140574	39.0	37.0	39.0	35.0	39.0
12	37.53244251075165	39.0	37.0	39.0	35.0	39.0
13	37.60080075742987	39.0	37.0	39.0	35.0	39.0
14	38.90580819933886	40.0	38.0	41.0	36.0	41.0
15	38.86032780505809	40.0	38.0	41.0	36.0	41.0
16	38.81553794370627	40.0	38.0	41.0	35.0	41.0
17	38.78833335339239	40.0	38.0	41.0	35.0	41.0
18	38.770500353039345	40.0	38.0	41.0	35.0	41.0
19	38.78955394681944	40.0	38.0	41.0	35.0	41.0
20	38.745521314750626	40.0	38.0	41.0	35.0	41.0
21	38.730264398388854	40.0	38.0	41.0	35.0	41.0
22	38.703863173181844	40.0	38.0	41.0	35.0	41.0
23	38.64348742698505	40.0	38.0	41.0	35.0	41.0
24	38.61322734129276	40.0	38.0	41.0	34.0	41.0
25	38.55527422732524	40.0	38.0	41.0	34.0	41.0
26	38.44978667196226	40.0	38.0	41.0	34.0	41.0
27	38.359901048687334	40.0	38.0	41.0	34.0	41.0
28	38.292113983567624	40.0	38.0	41.0	34.0	41.0
29	38.22797315296232	40.0	38.0	41.0	34.0	41.0
30	38.176203242345466	40.0	38.0	41.0	34.0	41.0
31	38.13197954778869	40.0	38.0	41.0	34.0	41.0
32	38.0759260262212	40.0	38.0	41.0	33.0	41.0
33	38.04492275258361	40.0	38.0	41.0	33.0	41.0
34	37.99331431735028	40.0	38.0	41.0	33.0	41.0
35	37.93813687495988	40.0	38.0	41.0	33.0	41.0
36	37.876331419699596	40.0	37.0	41.0	33.0	41.0
37	37.86251775226266	40.0	37.0	41.0	33.0	41.0
38	37.81584885904102	40.0	37.0	41.0	33.0	41.0
39	37.77517712144554	40.0	37.0	41.0	33.0	41.0
40	37.72997304063162	40.0	37.0	41.0	33.0	41.0
41	37.65102963123435	40.0	37.0	41.0	33.0	41.0
42	37.56667980534694	40.0	37.0	41.0	33.0	41.0
43	36.58234241607292	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	2.0
16	7.0
17	16.0
18	45.0
19	84.0
20	200.0
21	381.0
22	794.0
23	1391.0
24	2349.0
25	3624.0
26	5303.0
27	8090.0
28	11227.0
29	15754.0
30	22010.0
31	29126.0
32	38445.0
33	51214.0
34	71001.0
35	101877.0
36	154723.0
37	269982.0
38	602941.0
39	603521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96965265742346	16.7833602124655	12.39835074459208	27.848636385518965
2	18.788312792862186	19.052741270299762	35.12280152769754	27.036144409140512
3	20.519509435778932	20.709368380512228	28.71939991334489	30.05172227036395
4	15.835068441491751	14.387406524809037	32.55489160087297	37.22263343282624
5	16.699613662622763	34.301834601065536	33.10405834777585	15.89449338853585
6	37.936635454778866	32.41798855831568	14.232249743244111	15.41312624366134
7	31.614623451441044	28.888848770781177	19.56234153347455	19.934186244303227
8	28.085433516271905	34.339796360485266	18.538577572373065	19.03619255086976
9	28.154737547339366	13.962054287823353	16.975425653122795	40.90778251171449
10	17.345465049104565	25.573488349701524	32.02618508890173	25.054861512292188
11	38.85122801527697	20.031071474420695	19.611135181975737	21.506565328326595
12	22.98958132742795	22.83592897490211	28.871798575004814	25.302691122665127
13	32.79549995185827	18.10078872199756	22.68042115989473	26.423290166249437
14	23.651981433339753	18.329863117016494	23.81375770267668	34.20439774696707
15	27.250475399576352	25.230227790615572	20.75991719622569	26.759379613582386
16	26.339192582964248	24.2930687945311	22.87063113807048	26.497107484434174
17	26.023463075293662	24.109177418319533	23.590299842737018	26.277059663649787
18	26.195018133384686	23.95522417998588	23.956227132678606	25.893530553950832
19	27.351823769176452	23.83948343924514	23.5960668207202	25.212625970858205
20	26.697397137171837	23.161738157134604	23.57921721548238	26.561647490211183
21	26.19050484626741	23.936619407535787	23.873383240259326	25.999492505937482
22	26.984642788368955	23.407712305025996	22.876799297130752	26.73084560947429
23	26.31537245651197	23.364535191604084	23.616476908017205	26.703615443866745
24	26.184888311188136	23.222466942679247	23.658701216381026	26.933943529751588
25	26.735910520572563	22.950716910584763	23.369349364529175	26.9440232043135
26	26.23804480390269	23.32697461326144	23.895749085307145	26.539231497528725
27	27.029224035560688	23.519992858976828	23.41097190127736	26.03981120418512
28	26.34932240516079	24.146537406123628	23.056428124398227	26.44771206431735
29	26.508591292765903	23.968613598433787	23.474458806727007	26.048336302073306
30	26.914436099878042	23.596167115989473	23.311779879966622	26.177616904165863
31	26.47970625521535	23.238664628666793	23.56031155722447	26.721317558893382
32	25.836061364657553	23.515780457667372	23.660105350150847	26.988052827524236
33	26.176413360934593	23.133555186468964	23.783117497913857	26.906913954682587
34	26.471732781308173	23.168658530714424	24.350387540920472	26.009221147056937
35	26.27640774439951	23.13626315873933	24.13971732781308	26.44761176904808
36	25.911734145323834	22.77845978560883	24.650671577123052	26.659134491944286
37	25.470384812889147	22.413184414917517	25.005365796906094	27.111064975287245
38	25.727943064381538	21.97088227742474	25.224711550805573	27.07646310738815
39	24.949200446113355	21.87384660440336	26.218687816933052	26.95826513255023
40	25.583417581359523	21.573261682392967	26.51039660761281	26.332924128634698
41	24.83175468579498	21.557816210924962	26.844981625906666	26.76544747737339
42	24.04839848514025	21.712621959047436	27.367319388279093	26.871660167533218
43	23.80748924834713	21.42347069773413	27.2269561589319	27.542083894986842
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	14.0
2	14.0
3	51.5
4	89.0
5	89.0
6	123.0
7	157.0
8	148.0
9	139.0
10	189.5
11	240.0
12	240.0
13	392.5
14	545.0
15	928.0
16	1311.0
17	1318.0
18	1325.0
19	1325.0
20	1660.5
21	1996.0
22	2289.5
23	2583.0
24	3608.5
25	4634.0
26	4634.0
27	6352.0
28	8070.0
29	10389.5
30	12709.0
31	16440.5
32	20172.0
33	20172.0
34	25502.5
35	30833.0
36	37643.0
37	44453.0
38	53836.0
39	63219.0
40	63219.0
41	74463.0
42	85707.0
43	100014.0
44	114321.0
45	130969.0
46	147617.0
47	147617.0
48	166016.5
49	184416.0
50	196034.5
51	207653.0
52	211980.0
53	216307.0
54	216307.0
55	209308.0
56	202309.0
57	188455.0
58	174601.0
59	161816.5
60	149032.0
61	149032.0
62	130959.0
63	112886.0
64	94704.5
65	76523.0
66	63488.0
67	50453.0
68	50453.0
69	41051.5
70	31650.0
71	25648.0
72	19646.0
73	15935.5
74	12225.0
75	12225.0
76	9849.5
77	7474.0
78	5904.5
79	4335.0
80	3347.0
81	2359.0
82	2359.0
83	1766.0
84	1173.0
85	887.0
86	601.0
87	419.0
88	237.0
89	237.0
90	147.0
91	57.0
92	37.5
93	18.0
94	12.0
95	6.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1994112.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.33689658609939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.78374795953253	55.57953823987325
2	8.939235772088104	11.860023179435379
3	2.8394522286851234	5.650813465663634
4	1.3475356916836747	3.5756534330119134
5	0.7905668625286913	2.622187610198142
6	0.5192377668433479	2.0666773225610613
7	0.3427238036689955	1.5914663465109353
8	0.2528203926562379	1.3417056193995116
9	0.19453604507303338	1.1614425753850728
>10	0.9350542872014932	10.499754946881644
>50	0.03762641202321288	1.7039603408116495
>100	0.016556254754388718	1.8774660231886644
>500	7.554360509684123E-4	0.3380140951199409
>1k	1.510872101936803E-4	0.13129680195911567
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.014763463636947E-5
7	0.0	0.0	0.0	0.0	5.014763463636947E-5
8	0.0	0.0	0.0	0.0	5.014763463636947E-5
9	0.0	0.0	0.0	0.0	1.0029526927273894E-4
10	0.0	0.0	0.0	5.014763463636947E-5	1.0029526927273894E-4
11	0.0	0.0	0.0	5.014763463636947E-5	1.0029526927273894E-4
12	0.0	0.0	0.0	5.014763463636947E-5	1.504429039091084E-4
13	0.0	0.0	0.0	5.014763463636947E-5	1.504429039091084E-4
14	0.0	0.0	0.0	5.014763463636947E-5	2.5073817318184736E-4
15	0.0	0.0	0.0	5.014763463636947E-5	3.008858078182168E-4
16	0.0	0.0	0.0	5.014763463636947E-5	4.5132871172732527E-4
17	0.0	0.0	0.0	5.014763463636947E-5	4.5132871172732527E-4
18	0.0	0.0	0.0	5.014763463636947E-5	5.014763463636947E-4
19	0.0	0.0	0.0	2.0059053854547788E-4	5.014763463636947E-4
20	0.0	0.0	0.0	3.510334424545863E-4	5.516239810000642E-4
21	0.0	0.0	0.0	6.017716156364336E-4	5.516239810000642E-4
22	0.0	0.0	0.0	9.026574234546505E-4	5.516239810000642E-4
23	0.0	0.0	0.0	0.0011032479620001283	6.017716156364336E-4
24	0.0	0.0	0.0	0.0015545766737274536	6.017716156364336E-4
25	0.0	0.0	0.0	0.002005905385454779	6.017716156364336E-4
26	0.0	0.0	0.0	0.0033598915206367545	6.017716156364336E-4
27	0.0	0.0	0.0	0.014542814044547147	6.017716156364336E-4
28	0.0	0.0	0.0	0.05556357917709737	6.519192502728032E-4
29	0.0	0.0	0.0	0.12476731497528724	6.519192502728032E-4
30	0.0	0.0	0.0	0.21708911034084344	6.519192502728032E-4
31	0.0	0.0	0.0	0.6307569484562552	6.519192502728032E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1560	0.0	23.125	37
TAATACG	100	5.8821497E-6	16.65	27
TATTAAG	145	2.9831426E-9	16.586208	2
TCTTATA	2765	0.0	16.459312	37
TACTTAC	275	0.0	16.145454	31
ACTATCG	195	1.8189894E-12	16.128206	11
GTAACGT	150	4.6766218E-9	16.033333	26
TCTATAC	170	1.4879333E-9	15.235294	3
TTATACT	260	0.0	14.942307	4
TATACTG	300	0.0	14.8	5
GTATAGA	180	3.3342076E-9	14.388888	1
TTAGCCG	185	4.9039954E-9	14.0	4
CGGGTAA	175	3.5695848E-8	13.742858	23
GTCTTAG	310	0.0	13.725806	1
TAGTACT	135	6.5722925E-6	13.703704	4
TTATACG	95	0.001245635	13.631579	4
ATACCGT	110	2.457779E-4	13.454545	6
TTAGATG	290	0.0	13.396553	3
CGTATTA	125	4.847873E-5	13.32	2
GTATTAG	155	1.8893006E-6	13.129032	1
>>END_MODULE