##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211926.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	966769
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56308487342891	34.0	31.0	34.0	31.0	34.0
2	32.692475658611315	34.0	31.0	34.0	31.0	34.0
3	32.76721636709493	34.0	31.0	34.0	31.0	34.0
4	36.2212162367639	37.0	37.0	37.0	35.0	37.0
5	36.129151844959864	37.0	35.0	37.0	35.0	37.0
6	36.14838705006056	37.0	36.0	37.0	35.0	37.0
7	36.12591632540969	37.0	36.0	37.0	35.0	37.0
8	36.106735942091646	37.0	35.0	37.0	35.0	37.0
9	37.96603635408252	39.0	38.0	39.0	35.0	39.0
10	37.839015318033574	39.0	38.0	39.0	35.0	39.0
11	37.93821688531593	39.0	38.0	39.0	35.0	39.0
12	37.87019649988777	39.0	38.0	39.0	35.0	39.0
13	37.9258974998164	39.0	38.0	39.0	35.0	39.0
14	39.318289063881856	40.0	39.0	41.0	36.0	41.0
15	39.2901851424694	40.0	39.0	41.0	36.0	41.0
16	39.24494062180314	40.0	39.0	41.0	36.0	41.0
17	39.22570955419547	40.0	39.0	41.0	36.0	41.0
18	39.20924646942548	40.0	39.0	41.0	36.0	41.0
19	39.2175752428967	40.0	39.0	41.0	36.0	41.0
20	39.1719842071891	40.0	39.0	41.0	36.0	41.0
21	39.15692683567636	40.0	39.0	41.0	36.0	41.0
22	39.141629489567826	40.0	39.0	41.0	36.0	41.0
23	39.079239197781476	40.0	39.0	41.0	36.0	41.0
24	39.04409740072344	40.0	39.0	41.0	36.0	41.0
25	38.98878429076646	40.0	39.0	41.0	35.0	41.0
26	38.896782995731144	40.0	38.0	41.0	35.0	41.0
27	38.814502740571946	40.0	38.0	41.0	35.0	41.0
28	38.743533356986	40.0	38.0	41.0	35.0	41.0
29	38.68465165928986	40.0	38.0	41.0	35.0	41.0
30	38.638441034000884	40.0	38.0	41.0	35.0	41.0
31	38.58983686899352	40.0	38.0	41.0	35.0	41.0
32	38.53661733051018	40.0	38.0	41.0	34.0	41.0
33	38.495359284379205	40.0	38.0	41.0	34.0	41.0
34	38.446884416029064	40.0	38.0	41.0	34.0	41.0
35	38.401556111128926	40.0	38.0	41.0	34.0	41.0
36	38.33674952341252	40.0	38.0	41.0	34.0	41.0
37	38.31337889402742	40.0	38.0	41.0	34.0	41.0
38	38.26845916656409	40.0	38.0	41.0	34.0	41.0
39	38.211788958892974	40.0	38.0	41.0	34.0	41.0
40	38.17358334824555	40.0	38.0	41.0	34.0	41.0
41	38.09660425603221	40.0	38.0	41.0	33.0	41.0
42	38.0197834229273	40.0	37.0	41.0	33.0	41.0
43	37.04711156439646	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	5.0
18	17.0
19	29.0
20	73.0
21	150.0
22	306.0
23	547.0
24	877.0
25	1429.0
26	2070.0
27	3029.0
28	4216.0
29	5996.0
30	8222.0
31	11168.0
32	14795.0
33	19889.0
34	27943.0
35	39921.0
36	60107.0
37	102269.0
38	241304.0
39	422405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62820901373544	15.865113589699298	12.00203978406424	28.50463761250102
2	19.143145880763658	18.514040065413763	34.36518961613374	27.977624437688835
3	20.5575478733803	20.052566848957714	28.326828849497655	31.063056428164327
4	16.078194480791172	13.881599430680957	31.576622750626054	38.46358333790182
5	17.13325520367327	33.9295115999789	32.584205740978454	16.35302745536938
6	38.917052574089574	31.515801603071676	13.854809163305815	15.712336659532939
7	32.20376325678627	28.448367707280642	19.026675451943536	20.321193583989558
8	28.766644358683408	33.75159939964976	18.106910751172204	19.37484549049463
9	28.759093433902	13.380342149986191	16.34371809604983	41.51684632006198
10	17.651372768468992	25.19878068080379	31.372644344202183	25.777202206525036
11	39.655491642781264	19.47300751265297	19.022227646935306	21.84927319763046
12	23.38262811488577	22.300880561954305	28.244182426205228	26.0723088969547
13	33.54689693194548	17.626237498306214	21.962640506677396	26.864225063070908
14	24.099035033187867	17.88193456761646	23.088142048410738	34.93088835078493
15	27.911010799891184	24.566468308354946	20.201620035396253	27.32090085635762
16	26.902807185584148	23.730280966807996	22.31463772628208	27.052274121325777
17	26.773096779065114	23.312911357314935	22.768520711772926	27.14547115184703
18	26.841882600703993	23.565815618829316	23.183718137424762	26.40858364304193
19	28.13226323971911	23.155996934117663	22.84496089551899	25.86677893064424
20	27.368275151561544	22.60715848356743	22.7363517034576	27.28821466141343
21	26.841055102097812	23.365457518807492	23.27339829886974	26.520089080224956
22	27.826605942060617	22.559680751037735	22.079007498171745	27.534705808729903
23	26.992487347029126	22.692908026633045	22.938571675343333	27.3760329509945
24	26.642248561962578	22.50496240570395	23.041181502509904	27.81160752982357
25	27.488986510738343	22.103832456357207	22.71121643329482	27.695964599609628
26	26.967145202214798	22.590711948769563	23.222403697263772	27.219739151751867
27	27.86911868295322	22.877853965114728	22.719387982030867	26.533639369901184
28	26.94883679555302	23.615672409851783	22.22350944227628	27.211981352318908
29	27.016484806608403	23.47396327354311	22.862028054271498	26.64752386557699
30	27.70475677230031	22.871337413591046	22.57519634990365	26.848709464204994
31	27.086718750808103	22.602400366581882	22.9333998090547	27.377481073555316
32	26.39968803302547	22.98015348030398	22.962982884225706	27.657175602444845
33	26.55060309132792	22.486757436367945	23.223024321218407	27.73961515108573
34	26.94997460613652	22.551095452998595	23.90250411421963	26.59642582664525
35	26.727480918399326	22.4565537372423	23.512855708033666	27.303109636324706
36	26.29221665154758	21.955089581895987	24.411932943650445	27.34076082290599
37	25.792097181436308	21.57940521468934	24.79920229134364	27.829295312530704
38	26.152369387102816	21.050323293361703	24.948358915107953	27.84894840442753
39	25.225570948178934	21.182516195699282	26.11440788854421	27.47750496757757
40	26.067343905317607	20.71828947763116	26.248566100071475	26.965800516979755
41	25.025833472111746	20.80041871429473	26.862156316555453	27.311591497038073
42	24.284498158298415	20.957540012143543	27.363827346553315	27.394134483004727
43	23.691491969643213	20.554031004304026	27.246012232498146	28.508464793554612
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	6.0
2	7.0
3	17.5
4	28.0
5	28.0
6	39.5
7	51.0
8	43.0
9	35.0
10	51.0
11	67.0
12	67.0
13	118.0
14	169.0
15	267.5
16	366.0
17	390.5
18	415.0
19	415.0
20	507.5
21	600.0
22	756.0
23	912.0
24	1265.5
25	1619.0
26	1619.0
27	2179.0
28	2739.0
29	3527.0
30	4315.0
31	5671.0
32	7027.0
33	7027.0
34	9007.5
35	10988.0
36	13688.0
37	16388.0
38	20246.0
39	24104.0
40	24104.0
41	29203.5
42	34303.0
43	40857.5
44	47412.0
45	56235.5
46	65059.0
47	65059.0
48	74973.0
49	84887.0
50	93217.5
51	101548.0
52	105182.5
53	108817.0
54	108817.0
55	105252.5
56	101688.0
57	96614.5
58	91541.0
59	85720.0
60	79899.0
61	79899.0
62	70759.5
63	61620.0
64	52162.5
65	42705.0
66	35583.5
67	28462.0
68	28462.0
69	23469.5
70	18477.0
71	15272.5
72	12068.0
73	9930.5
74	7793.0
75	7793.0
76	6267.5
77	4742.0
78	3843.0
79	2944.0
80	2232.5
81	1521.0
82	1521.0
83	1189.0
84	857.0
85	612.5
86	368.0
87	264.5
88	161.0
89	161.0
90	102.5
91	44.0
92	26.0
93	8.0
94	6.5
95	5.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	966769.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.27170100216325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.78334327943614	63.5876318073894
2	7.65061959745332	11.211478232517806
3	2.3335530075055013	5.129501947159257
4	1.0584218384586386	3.1020947392680522
5	0.6099771017846723	2.234702991006631
6	0.38736953023477333	1.7029934638026447
7	0.2473235195879569	1.268527048463601
8	0.18021907412070232	1.056396649108704
9	0.12623468108294006	0.8324486827571356
>10	0.5873892735911249	7.267941553191353
>50	0.02483197291472063	1.2728878974708406
>100	0.010717123829527737	1.333394987864618
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.068746515455088E-4
2	0.0	0.0	0.0	0.0	3.1031197731826323E-4
3	0.0	0.0	0.0	0.0	3.1031197731826323E-4
4	0.0	0.0	0.0	0.0	3.1031197731826323E-4
5	0.0	1.034373257727544E-4	0.0	0.0	3.1031197731826323E-4
6	0.0	1.034373257727544E-4	0.0	0.0	4.137493030910176E-4
7	0.0	1.034373257727544E-4	0.0	0.0	4.137493030910176E-4
8	0.0	1.034373257727544E-4	0.0	0.0	4.137493030910176E-4
9	0.0	1.034373257727544E-4	0.0	1.034373257727544E-4	5.17186628863772E-4
10	0.0	1.034373257727544E-4	0.0	2.068746515455088E-4	5.17186628863772E-4
11	0.0	1.034373257727544E-4	0.0	2.068746515455088E-4	5.17186628863772E-4
12	0.0	1.034373257727544E-4	0.0	2.068746515455088E-4	5.17186628863772E-4
13	0.0	1.034373257727544E-4	0.0	2.068746515455088E-4	5.17186628863772E-4
14	0.0	1.034373257727544E-4	0.0	2.068746515455088E-4	6.206239546365265E-4
15	0.0	2.068746515455088E-4	0.0	2.068746515455088E-4	0.001034373257727544
16	0.0	2.068746515455088E-4	0.0	3.1031197731826323E-4	0.0011378105835002984
17	0.0	2.068746515455088E-4	0.0	4.137493030910176E-4	0.0011378105835002984
18	0.0	2.068746515455088E-4	0.0	4.137493030910176E-4	0.001241247909273053
19	0.0	2.068746515455088E-4	0.0	4.137493030910176E-4	0.001241247909273053
20	0.0	2.068746515455088E-4	0.0	5.17186628863772E-4	0.0013446852350458072
21	0.0	2.068746515455088E-4	0.0	7.240612804092808E-4	0.0013446852350458072
22	0.0	2.068746515455088E-4	0.0	8.274986061820352E-4	0.0013446852350458072
23	0.0	2.068746515455088E-4	0.0	0.0017584345381368247	0.0016549972123640704
24	0.0	2.068746515455088E-4	0.0	0.00258593314431886	0.0016549972123640704
25	0.0	2.068746515455088E-4	0.0	0.003103119773182632	0.0016549972123640704
26	0.0	2.068746515455088E-4	0.0	0.005378740940183229	0.0016549972123640704
27	0.0	2.068746515455088E-4	0.0	0.019446217245277828	0.0016549972123640704
28	0.0	2.068746515455088E-4	0.0	0.06744113640383587	0.0016549972123640704
29	0.0	2.068746515455088E-4	0.0	0.1574316098261322	0.0016549972123640704
30	0.0	2.068746515455088E-4	0.0	0.269971420266889	0.0016549972123640704
31	0.0	2.068746515455088E-4	0.0	0.7469209294050595	0.0017584345381368247
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTAAT	55	1.9022536E-5	23.545454	2
CTTATAC	735	0.0	22.904762	37
ATAGAAT	45	0.003825375	20.555555	3
TAGGCCG	45	0.003825375	20.555555	5
TAATACG	80	1.616237E-5	18.5	27
CGCGGTA	90	2.151497E-6	18.5	22
GCATTAA	80	1.616237E-5	18.5	1
CAATAGG	50	0.0070340927	18.5	2
TAGTACT	70	1.218943E-4	18.5	4
TCTATAC	50	0.0070340927	18.5	3
TCTTATA	1475	0.0	18.061018	37
ATACCGT	75	2.0668386E-4	17.266666	6
GGTAATA	100	5.875845E-6	16.650002	25
GTAATAC	100	5.875845E-6	16.650002	26
ACTCCGT	135	2.2178938E-8	16.444443	6
GTATTAG	80	3.381869E-4	16.1875	1
ATTAATG	70	0.002592094	15.857142	3
TACTAGT	70	0.002592094	15.857142	4
GTCTTAT	70	0.002592094	15.857142	1
CGGTAAT	105	9.339947E-6	15.857142	24
>>END_MODULE