FastQCFastQC Report
Thu 9 Feb 2017
SRR3211925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009253
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15450.021.67313837
TATACCG650.001580489817.0769235
TCTTATA28150.016.88987537
CGTTACA904.448621E-516.44444524
GGTATCA13150.016.1787071
TACTTAC2750.016.14545431
TCTATAC1601.0977601E-815.031253
ATAGCGC750.004105828214.7999993
CTATACA1203.302774E-513.8750014
GTAGGAC2009.840733E-1013.8750013
CTCGTTA4200.013.65476122
TAGAGTA1409.566527E-613.2142864
ATTAGAT1409.566527E-613.2142863
TCGTTAT850.00940793313.05882423
GTATTGA2602.3646862E-1112.8076931
CGTATAC1307.002895E-512.8076933
GTCCTAT2052.0676453E-812.6341461
GTATAAG2052.0676453E-812.6341461
TCGTTAC4400.012.61363623
CTAAGGT2204.089088E-912.6136364
Produced by FastQC (version 0.11.5)