##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211922.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3127129
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43204933343012	34.0	31.0	34.0	31.0	34.0
2	32.569056153423794	34.0	31.0	34.0	31.0	34.0
3	32.645295732923074	34.0	31.0	34.0	31.0	34.0
4	36.14402699728729	37.0	35.0	37.0	35.0	37.0
5	36.02951653097777	37.0	35.0	37.0	35.0	37.0
6	36.0427686225928	37.0	35.0	37.0	35.0	37.0
7	36.02771232015053	37.0	35.0	37.0	35.0	37.0
8	36.00490417888101	37.0	35.0	37.0	35.0	37.0
9	37.850463796025046	39.0	38.0	39.0	35.0	39.0
10	37.71570888185297	39.0	37.0	39.0	35.0	39.0
11	37.81596441976011	39.0	38.0	39.0	35.0	39.0
12	37.75088267864869	39.0	37.0	39.0	35.0	39.0
13	37.80484111784324	39.0	38.0	39.0	35.0	39.0
14	39.16613865305845	40.0	39.0	41.0	36.0	41.0
15	39.12692920567076	40.0	39.0	41.0	36.0	41.0
16	39.07959889086763	40.0	38.0	41.0	36.0	41.0
17	39.06570243824287	40.0	38.0	41.0	36.0	41.0
18	39.04190744929294	40.0	38.0	41.0	36.0	41.0
19	39.05459288695798	40.0	39.0	41.0	36.0	41.0
20	39.00104312933684	40.0	39.0	41.0	36.0	41.0
21	38.9878831349778	40.0	38.0	41.0	36.0	41.0
22	38.96075633592346	40.0	38.0	41.0	35.0	41.0
23	38.89501264578468	40.0	38.0	41.0	35.0	41.0
24	38.86253301350855	40.0	38.0	41.0	35.0	41.0
25	38.804974786777265	40.0	38.0	41.0	35.0	41.0
26	38.698671209278544	40.0	38.0	41.0	35.0	41.0
27	38.61627134665695	40.0	38.0	41.0	35.0	41.0
28	38.54705642140123	40.0	38.0	41.0	34.0	41.0
29	38.478015777411166	40.0	38.0	41.0	34.0	41.0
30	38.427292893897246	40.0	38.0	41.0	34.0	41.0
31	38.36243052333306	40.0	38.0	41.0	34.0	41.0
32	38.297355177864425	40.0	38.0	41.0	34.0	41.0
33	38.25510748037577	40.0	38.0	41.0	34.0	41.0
34	38.18931102618408	40.0	38.0	41.0	34.0	41.0
35	38.1372063640483	40.0	38.0	41.0	33.0	41.0
36	38.049919590781194	40.0	38.0	41.0	33.0	41.0
37	38.02608398949963	40.0	38.0	41.0	33.0	41.0
38	37.96909177715406	40.0	37.0	41.0	33.0	41.0
39	37.91375187911979	40.0	37.0	41.0	33.0	41.0
40	37.86419779932328	40.0	37.0	41.0	33.0	41.0
41	37.77417848767991	40.0	37.0	41.0	33.0	41.0
42	37.69211375673981	40.0	37.0	41.0	33.0	41.0
43	36.72275528128197	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	0.0
13	1.0
14	3.0
15	2.0
16	7.0
17	25.0
18	52.0
19	151.0
20	273.0
21	630.0
22	1217.0
23	2129.0
24	3579.0
25	5411.0
26	8088.0
27	11675.0
28	16089.0
29	22238.0
30	30114.0
31	40517.0
32	53724.0
33	71834.0
34	100400.0
35	144220.0
36	220248.0
37	368245.0
38	825945.0
39	1200309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.529128475352316	16.09533856774057	12.078523143752625	28.297009813154496
2	19.322420021687627	18.607706941414953	34.911223681530245	27.158649355367174
3	20.478208606040877	19.95798702260124	28.79593390614842	30.767870465209462
4	16.291237105984436	14.140478374892753	31.698436489188648	37.86984802993417
5	17.20024981380685	33.887920837291965	32.38951766940219	16.52231167949899
6	38.115440712551354	31.67458074163234	14.267847600786537	15.94213094502977
7	31.875659750525166	29.03190114638699	18.95329549884255	20.139143604245298
8	28.402282093255504	34.11506209049898	18.116329706897286	19.366326109348222
9	29.080540009702187	13.567876477113671	16.627775828883298	40.72380768430084
10	17.896543442883235	24.97095578724127	30.950466066478228	26.18203470339727
11	39.41900062325539	19.94014317925484	19.28458979466469	21.35626640282508
12	23.289861083441075	22.209796909561454	27.93236224025296	26.567979766744514
13	32.989205114339704	17.98211714323266	21.974501211814417	27.054176530613223
14	23.884304101301865	18.110701541253974	23.155072911926563	34.849921445517595
15	28.008374454651534	24.72363628107443	20.13102113791916	27.136968126354876
16	26.608592098375215	24.148476126184754	22.27432894517623	26.968602830263798
17	26.537312659631247	23.38851387326842	22.712622344649038	27.361551122451296
18	26.6399627261939	23.97927939653273	23.075734963284216	26.305022913989156
19	28.496330020283782	23.139051826771457	22.79295161792174	25.57166653502302
20	27.265488567948427	22.629478988554677	22.858123217814168	27.246909225682725
21	26.76525336818532	23.509871194952304	23.621986812824158	26.10288862403822
22	28.01377877279767	22.234068373898232	22.191601305862342	27.560551547441758
23	26.78009125942678	23.02968633529349	23.36254756359587	26.82767484168386
24	26.389701224349878	22.315548862870706	23.46877919011336	27.82597072266606
25	27.20079664126424	22.191569327648462	22.94510395957442	27.66253007151288
26	26.611182333699695	22.595837907550344	23.690676016243653	27.102303742506308
27	27.533114239930622	23.045291703668124	22.790233469741732	26.631360586659518
28	26.770849555614753	23.92347741330786	21.97968168246337	27.325991348614014
29	26.690136543775456	23.79335806102019	23.1615644893447	26.354940905859657
30	28.070508124225128	22.853358463945682	22.319546139606008	26.756587272223182
31	27.052513663491336	22.818054515819462	23.209467853740602	26.919963966948597
32	26.324433689815802	23.191336206469256	23.24410025937529	27.24012984433965
33	26.371281773153587	22.280244914744483	23.414192379016026	27.93428093308591
34	26.898410650791828	22.444005348036487	24.463333620071317	26.194250381100364
35	26.669862356173983	21.993048575866233	23.949795483333112	27.387293584626665
36	26.00465155099134	21.655390615481483	24.96695851050596	27.37299932302121
37	25.435023627103327	21.36563601949264	25.51624829036474	27.683092063039293
38	25.95639642624273	20.85606318127586	25.42808435469084	27.759456037790574
39	24.799520582617475	21.125703480732646	26.912832825252814	27.16194311139707
40	25.925633384487817	20.412781180437392	26.826491647770208	26.835093787304586
41	24.490003450449276	21.18169733324081	27.280582284900945	27.04771693140897
42	23.959452903925612	21.23139147761413	27.6389941060954	27.170161512364853
43	23.21506404117003	20.856990549478454	27.301272189282887	28.626673220068632
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	41.0
2	60.0
3	104.5
4	149.0
5	149.0
6	178.0
7	207.0
8	212.0
9	217.0
10	295.5
11	374.0
12	374.0
13	604.0
14	834.0
15	1378.5
16	1923.0
17	2055.0
18	2187.0
19	2187.0
20	2820.5
21	3454.0
22	4134.0
23	4814.0
24	6596.0
25	8378.0
26	8378.0
27	11201.0
28	14024.0
29	17695.5
30	21367.0
31	27259.5
32	33152.0
33	33152.0
34	41170.0
35	49188.0
36	59949.5
37	70711.0
38	84177.0
39	97643.0
40	97643.0
41	113021.0
42	128399.0
43	147671.0
44	166943.0
45	193160.0
46	219377.0
47	219377.0
48	243724.0
49	268071.0
50	286893.5
51	305716.0
52	308677.0
53	311638.0
54	311638.0
55	297077.5
56	282517.0
57	268060.0
58	253603.0
59	239634.5
60	225666.0
61	225666.0
62	203087.5
63	180509.0
64	158780.5
65	137052.0
66	120814.0
67	104576.0
68	104576.0
69	91535.5
70	78495.0
71	67803.5
72	57112.0
73	48721.5
74	40331.0
75	40331.0
76	33593.5
77	26856.0
78	21368.0
79	15880.0
80	12311.0
81	8742.0
82	8742.0
83	6332.5
84	3923.0
85	2928.0
86	1933.0
87	1341.0
88	749.0
89	749.0
90	492.0
91	235.0
92	151.5
93	68.0
94	43.0
95	18.0
96	18.0
97	14.0
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3127129.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.35116852509763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0423478679314	53.43872122369036
2	9.297163667478497	11.965666919426472
3	2.9027036769158387	5.60377120474895
4	1.4563054496073238	3.748598296467959
5	0.8322008991081702	2.6776550152623817
6	0.5371719222447514	2.0740584537193585
7	0.37080881266297533	1.670338627598654
8	0.26127742043750596	1.3450805851501386
9	0.2042209948491558	1.182767369031101
>10	1.0362272721088368	11.498142889304816
>50	0.03967763962760884	1.7399309361412958
>100	0.018004641794316316	2.065934211993049
>500	0.0015913559866306143	0.6950781270369176
>1k	2.983792470149578E-4	0.2942561404287109
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.197821388244617E-5
2	0.0	0.0	0.0	3.197821388244617E-5	3.197821388244617E-5
3	0.0	0.0	0.0	3.197821388244617E-5	3.197821388244617E-5
4	0.0	0.0	0.0	3.197821388244617E-5	3.197821388244617E-5
5	0.0	0.0	0.0	3.197821388244617E-5	3.197821388244617E-5
6	0.0	0.0	0.0	3.197821388244617E-5	6.395642776489234E-5
7	0.0	0.0	3.197821388244617E-5	3.197821388244617E-5	6.395642776489234E-5
8	3.197821388244617E-5	0.0	3.197821388244617E-5	6.395642776489234E-5	1.2791285552978467E-4
9	3.197821388244617E-5	0.0	3.197821388244617E-5	9.59346416473385E-5	1.91869283294677E-4
10	6.395642776489234E-5	0.0	3.197821388244617E-5	1.2791285552978467E-4	1.91869283294677E-4
11	6.395642776489234E-5	0.0	3.197821388244617E-5	1.91869283294677E-4	2.2384749717712317E-4
12	6.395642776489234E-5	0.0	3.197821388244617E-5	2.2384749717712317E-4	2.2384749717712317E-4
13	6.395642776489234E-5	0.0	3.197821388244617E-5	2.878039249420155E-4	2.2384749717712317E-4
14	6.395642776489234E-5	0.0	3.197821388244617E-5	3.1978213882446165E-4	2.878039249420155E-4
15	6.395642776489234E-5	3.197821388244617E-5	3.197821388244617E-5	3.5176035270690785E-4	4.1571678047180015E-4
16	9.59346416473385E-5	3.197821388244617E-5	3.197821388244617E-5	3.83738566589354E-4	4.796732082366925E-4
17	9.59346416473385E-5	3.197821388244617E-5	3.197821388244617E-5	3.83738566589354E-4	4.796732082366925E-4
18	9.59346416473385E-5	3.197821388244617E-5	3.197821388244617E-5	3.83738566589354E-4	5.75607849884031E-4
19	9.59346416473385E-5	3.197821388244617E-5	3.197821388244617E-5	3.83738566589354E-4	5.75607849884031E-4
20	1.2791285552978467E-4	3.197821388244617E-5	6.395642776489234E-5	5.75607849884031E-4	6.395642776489233E-4
21	1.2791285552978467E-4	3.197821388244617E-5	9.59346416473385E-5	7.994553470611541E-4	6.715424915313695E-4
22	1.2791285552978467E-4	3.197821388244617E-5	9.59346416473385E-5	0.0012471503414154005	6.715424915313695E-4
23	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.0015989106941223083	8.953899887084927E-4
24	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.002686169966125478	8.953899887084927E-4
25	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.003453647099304186	8.953899887084927E-4
26	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.005947947782134987	9.273682025909388E-4
27	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.02810885000267018	9.273682025909388E-4
28	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.11237144358291583	9.273682025909388E-4
29	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.2345282206138602	9.273682025909388E-4
30	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	0.38760793046913	9.273682025909388E-4
31	1.91869283294677E-4	3.197821388244617E-5	9.59346416473385E-5	1.000054682745739	9.273682025909388E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1370	0.0	25.927008	1
CTTATAC	3700	0.0	20.7	37
TACTTAC	1000	0.0	20.164999	31
GTACTTA	1130	0.0	18.827435	30
ACATATA	355	0.0	16.676058	36
ACTTACA	935	0.0	16.62032	32
GTATCAA	2195	0.0	16.266516	2
CTCGTTA	1515	0.0	15.9967	22
AGTGTAC	1380	0.0	15.550725	27
TCTTATA	6270	0.0	15.549442	37
TTACATA	520	0.0	15.298077	34
GTCTAAT	135	3.9782026E-7	15.074075	1
TCGTTAC	1505	0.0	14.996676	23
CAGTGTA	1525	0.0	14.8	26
TATACTG	305	0.0	14.557377	5
ATTATAC	180	3.3378456E-9	14.388889	3
GTATTAT	245	0.0	14.34694	1
CTTACAT	685	0.0	14.3138685	33
TACGGAT	1995	0.0	14.280702	27
TACATAT	550	0.0	14.127272	35
>>END_MODULE